Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation and Characterization of a Naphthalene-Degrading Strain,Alcaligenes sp,A111

Naphthalene 분해균주 Alcaligenes sp. A111의 분리 및 특성

  • 오희목 (한국과학기술연구원 유전공학연구소) ;
  • 강정현 (한국과학기술연구원 유전공학연구소) ;
  • 이창호 (한국과학기술연구원 유전공학연구소) ;
  • 박찬선 (한국과학기술연구원 유전공학연구소) ;
  • 안성구 (한국과학기술연구원 유전공학연구소) ;
  • 윤병대 (한국과학기술연구원 유전공학연구소) ;
  • 고영희 (한국과학기술연구원 유전공학연구소)
  • Published : 1994.08.01
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Abstract

A bacterial strain which formed a distinct colony on agar plate containing naphthalene as a vapor phase and grew well ina liquid minimal medium was isolated and identified as Alcaligenes sp. A111. Optimum temperature and pH for the cultivation of Alcaligenes sp. A111 were 30$\cir$C and 7.0, respectively. Cell growth increased dramatically from 12 hours after inoculation and revealed a stationary phase at about 48 hours. Relative growth rate ($\mu$')increased hyperbolically depending on the conceration of naphthalene up to 500 ppm and reached to the maximum value pf 2.8/day, but $\mu$' didn't change within a range of 500~4000 ppm naphthalene. NH$_{4}$Cl or NH$_{4}$NO$_{3}$ was preferrd as a nitrogen source and a P : N ratio by weight og 6 : 1 was favorable to cell growth. Alcaligenes sp. A111 utilized the intermediates of degradation of naphthalene and showed tolerance to benzene, toluene, and octane. therefore, it is suggested that Alcaligenes sp. A111 could be effectively used for the biological treatment of wastewater containing naphthalene in the presence of some aromatic compounds.

Keywords

References

  1. Appl. Environ. Microbiol. v.53 Naphthalene biodegradation in environmental microcosms: estimates of degradation rates and characterization of metabolites Heitkamp,M.A.;J.P.Freeman;C.E.Cerniglia
  2. Environ. Sci. Technol. v.13 Priority polutants. I-a perspective view Keith,L.H.;W.A.Telliard
  3. Appl. Environ. Microbiol. v.54 Microbial degradation of acenaphthene and naphthalene under denitrification conditions in soil-water systems Mihelcic,J.R.;R.G.Luthy
  4. Appl. Environ. Microbiol. v.55 Seasonal biotransformation of naphthalene, phenanthrene, and benzo[α]pyrene in surficial estuarine sediments Shiaris,M.P.
  5. Environ. Sci. Technol. v.16 Microbial removal of hazardous organic compounds Kobayashi,H.;B.E.Rittmann
  6. Kor. Jour. Microbiol. v.28 Development of versatile strains of Pseudomonas degrading various persistent aromatic hydrocarbons Lee,G.H.;I.S.Choi;K.Y.Park;Y.K.Park;Y.N.Lee
  7. Appl. Microbiol. Biotechnol. v.38 Effect of 2-hydroxybenzoate on the rate of naphthalene mineralization in soil Ogunseitan,O.A.;B.H.Olson
  8. J. Bacteriol. v.149 Oxidation of naphthalene by a multicomponent enzyme system from Pseudomonas sp. strain NCIB 9816 Ensley,B.D.;D.T.Gibson;A.L.Laborde
  9. 산업미생물학회지 v.16 Naphthalene을 분해하는 Pseudomonas putida N3의 분리 및 특성 고영희;하일호;배경숙
  10. Appl. Environ. Microbiol. v.52 Rates of dissolution and biodegradation of water-insoluble organic compounds Thomas,J.M.;J.R.Yordy;J.A.Amador;M.Alexander
  11. 산업미생물학회지 v.17 Pseudomonas 속 세균에 의한 방향족화합물 생분해 정윤창;김경남;최용진;양한철;송준상;서윤수
  12. Appl. Microbiol. Biotechnol. v.39 Degradation of polycyclic aromatic hydrocarbons by an immobilized mixed bacterial culture Wiesel,I.;S.M.Wubker;H.J.Rehm
  13. Environ. Prog. v.10 Bioreactor design effects on biodegradation capabilities of VOCs in wastewater Hill,G.A.;M.E.Tomusiak;B.Quail;K.M.Van Cleave
  14. Bergey's Manual of Determinative Bacteriology(9th ed.) Holt,J.G.;N.R.Krieg;P.H.A.Sneath;J.T.Staley;S.T.Williams
  15. Manual for the Identification of Medical Bacteria(2nd ed.) Cowan,S.T.
  16. Appl. Environ. Microbiol. v.56 Degradation of pentachlorophenol by a Flavobacterium species grown in continuous culture under various nutrient limitations Topp,E.;R.S.Hanson
  17. Appl. Environ. Microbiol. v.43 Rapid screen for bacteria degrading water-insoluble, solid hydrocarbons on ager plates Kiyohara,H.;K.Nagao,K.Yana
  18. Recherches sur la Croissance des Cultures Bacteriennes(2nd ed.) Monod,J.