• Title/Summary/Keyword: SP-색인

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The Fruit-body Formation and Properties of Pholiota sp. (비늘버섯속균(Pholiota sp.)의 특징과 자실체 형성)

  • Sung, Jae-Mo;Lee, Jae-Keun;Park, Dong-Soo
    • The Korean Journal of Mycology
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    • v.26 no.2 s.85
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    • pp.194-199
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    • 1998
  • Pholiota sp. were collected from the forest of Kangwon province (Mt. Samak, Mt. Odae and Kangwon National University Forest Experimentation) from the early of August to the end of October 1997. Among these fungi, Poliota adiposa was selected for culture experiment because it is suitable for edible mushroom. The Optimal temperature for the mycelial growth of Pholiota adiposa was in the range of $28^{\circ}C$ while that of fruit body formation was $15{\pm}1^{\circ}C$. Brown sugar as carbon source and soybean flour as nitrogen source were good for mycelial growth in commercial liquid culture. The fruit bodies of Pholiota adiposa were artifitially produced on plastic bottle including poplar sawdust and rice bran (4:1).

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Physiologic Races of Puccinia graminis f. tritici in Korea (한국에 분포한 맥류 줄기녹병균(Puccinia graminis f. sp. tritici)의 생리적 품종)

  • Chung Bong Koo;Lee Ji Young
    • Korean journal of applied entomology
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    • v.12 no.2
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    • pp.79-82
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    • 1973
  • 1. In order to investigate Physiologic races of Puccinia gramininis f. sp. tritici as basic materials for tile breeding of resistant varieties to stem rust of wheat, this experiment was carried out during 1971 and 1973. 2. Twenty seven isolates of the fungus were identified as follows: two isolates of race 11, eight . isolates of race 17, and 21, four isolates of race 34, one each of race 37 and 56, respectively The remaining three isolates identified were not identical with the key issued by Stakman, et at in 1962. Therefore, the three new races were tentatively numbered $K_3,\;K_4\; and\; K_4,$ in addition to $K_l\;and\;K_2$ described in 1961. 3. Therefore, attempts to produce resistant varieties to stem rust of wheat should be accompanied by intensive investigation of Physiologic races of p. graminis f. sp. tritici in the future.

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Molecular Cloning of a Gene Cluster for Phenanthrene Degradation from Pseudomonas sp. Strain DJ77 and Its Expression in Escherichia coli (Pseudomonas sp. strain DJ77로 부터 phenanthrene 분해 유전자군의 클로닝과 대장균에서의 발현)

  • 김영창;윤길상;신명수;김흥식;박미선;박희진
    • Korean Journal of Microbiology
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    • v.30 no.1
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    • pp.1-7
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    • 1992
  • We cloned a gene cluster encoding phenanthrene-degrading enzymes on a 6.8-kb Xhol fragment from the Pseudomonas sp. DJ77 chromosomal DNA into the vector pBLUESCRIPT SIC(+). The resultant clone, containing the recombinant plilsmid pHENX7, was able to convert 3-methylcatechol to a yellow mela-cleavage compound. Since the pHENX7R in which the DNA insert was cloned in the opposite orientation lacked extradiol dioxygenase activity. the direction of transcription was established. Four polypeptides, PhnC (24 kDa). PhnD (31 kDa), PhnE (34 kDa). and PhnF (15 kDa), were identified in E coli JM101 transformed with several pHENX7-derived plasmids. The locations and extents of ~ndividual genes were determined by subcloning. The gene order was phnC-phnD-phnE-phnF-phnG, and phnC, phnD, phnE, and phnG genes encoded glutathione S-transferase, mrta-cleavage compound hydrolase, extradiol dioxygenase, mera-cleavage compound dehydrogenase, respectively.

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Multi-responsive hydrogel cross-linked synthesized spiropyran-based hydrophilic cross-linker (스피로피란 기반 친수성 가교제를 활용한 다중 자극 감응형 하이드로젤)

  • Jeong, Hye-Won;Kim, Sang Jin;Heo, Eun-Jin;Shin, Sung Gyu;Han, Sa Ra;Jeong, Jae Hyun
    • Journal of the Korean Applied Science and Technology
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    • v.38 no.1
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    • pp.126-135
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    • 2021
  • Stimuli-responsive hydrogels are being extensively studied to alter their physiochemical properties in response to external changes such as temperature, pH, light and mechanical stress. This study reports multi-responsive hydrogel having optical change response to external stress. First, we synthesized a novel spiropyran cross-linker successfully by grafting poly(ethylene glycol) diacrylate (PEGDA) on both side of spiropyran and introduced to hydrogel. In the results, the yellow spriopyran structure was conversed to purple merocyaine structure by internal stress during swelling of the hydrogels cross-linked with the SP-PEGDAs. Also, the hydrogel could be visualized the swelling and deswelling process in response to pH, by converting MC and prontonated MC structure.

Sexual Dimorphism of Three Species of Gymnogobius (Gobiidae) from Korea (한국산 날망둑속 3종 (망둑어과)의 성적이형)

  • Kim, Young-Ja;Kim, Jong-Man
    • Korean Journal of Ichthyology
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    • v.13 no.2
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    • pp.117-122
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    • 2001
  • Three species, Gymnogobius urotaenia, G. sp. 1 and G. sp. 2, collected at Hosan-river, Hosan-ri, Samcheock-si, Gangwon-do, Korea, were examined for the study of sexual dimorphism. The three species have peculiar nuptial pigmentation during the breeding season: all females of the three species showed an intense black coloration on the ventral part of the head, the pelvic fin, the anal fin and the margin of the first dorsal fin. There was a difference between G. sp. 1 and the other two species in 1/3 from margin of first dorsal fin. Namely, G. sp. 1 showed an intense black coloration, whereas the other two species had an intense yellow coloration. All females of the three species showed the same, intensely yellow coloration in the ventral part of the body. Males of G. urotaenia and G. sp. 2 had almost the same nuptial pigmentation, however the former showed diffuse black coloration in the anal fin, whereas the latter had an intensely black anal fin. Males of G. sp. 1 differed from those of the other two species in their yellow color on the ventral part of the head, the pelvic f ins, the anal fin, in contrast to the diffuse black coloration in those parts in the other species. The genital papilla of males was triangle-shaped, but that of females was heart-shaped. Significant differences between sexes include: G. urotaenia, 7 characters (body depth, head length, snout length, upper jaw length, head depth, pectoral fin ray length, eye diameter); G. sp. 1, 11 characters (body depth, head length, caudal peduncle length, caudal peduncle depth, snout length, upper jaw length, head width, head depth, anal fin ray length, pectoral fin ray length and caudal fin ray length); G. sp. 2, 10 characters (body depth, head length, caudal peduncle depth, snout length, upper jaw length, head width, anal fin ray length, pectoral f in ray length, caudal fin ray length and eye diameter). Of the 5 characters which showed differences in all three species (body depth, head length, snout length, upper jaw length and pectoral fin ray length), females had higher values than males only in body depth, whereas males values in all other characters. The upper jaw length was the most distinctive sexually dimorphic feature, the upper jaw extended beyond the posterior margin of the eyes (p<0.001) in males but not in females.

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Effect of Supplementary Actinomycetes (Nocardia sp. CS682) Ferment on the Laying Performance, Blood Parameters, Immunoglobulin and Small Intestinal Microflora Contents in Laying Hens (방선균(Nocardia sp. CS682) 발효물의 급여가 산란계의 생산성, 혈액성상, 면역글로불린 및 소장내 미생물 함량에 미치는 영향)

  • Rhee, Ah-Reum;Shin, Dong-Hun;Kim, Chan-Ho;Jung, Byoung-Yun;Yoo, Jin-Chul;Hong, Young-Ho;Paik, In-Kee
    • Korean Journal of Poultry Science
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    • v.38 no.1
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    • pp.59-69
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    • 2011
  • This study was conducted to investigate the effects of dietary supplementation of CS682, a fermentation product of Actinomycetae(Nocardia sp. CS682), and its commercial product DSC682$^{(R)}$ on the performance, blood parameters, intestinal microflora, and immune response in laying hens. Hy-Line Brown$^{(R)}$ laying hens were housed in two bird cages. Feeding trial lasted 5 wk under 16.5 h:7.5 h(L:D) lighting regimen. In Exp.1, a total of 480 birds of 86 wk old were assigned to four dietary treatments: Control, Antibiotics (6 ppm avilamycin), CS682-0.1 (CS682 0.1%) and CS682-1.0 (CS682 1.0% supplementation). Each treatment was replicated five times with 24 birds (or 12 cages) per replication. In Exp. 2, a total of 1,000 birds of 26 wk old were assigned to five dietary treatments: Control, Antibiotics (6 ppm avilamycin), DCS682-0.05 (DCS682 0.05%), DCS682-0.1 (DCS682 0.1%), DCS682-0.2 (DCS682 0.2% supplementation). Each treatment was replicated five times with 40 birds (or 20 cages) per replication. In Exp. 1, there were no significant differences among treatments in egg production, egg weight, broken & soft egg production, feed intake, and feed conversion ratio. Also, there were no significant differences among treatments in eggshell thickness, eggshell color and Haugh unit. However, eggshell strength was significantly (p<0.05) greater in CS682 and Antibiotics treatments than Control, and egg yolk color was significantly (p<0.05) higher in CS682-1.0 than Control. In Exp. 2, feed intake was significantly (p<0.05) lower in DSC682-0.05 than Control. Lightness(L) of Hunter Lab color of eggshell of DCS and Antibiotics treatments was significantly (p<0.05) lower than Control. Egg yolk color of DCS 0.1 and 0.2 treatments was significantly (p<0.05) higher than Control. Haugh unit increased significantly (p<0.05) in Antibiotics and DCS682-0.1 treatments. The immunoglobulin levels of plasma (IgG and IgA) and eggyolk (IgY) were not significantly affected by treatments. Antibiotics and CS682 or DCS682 treatments significantly (p<0.05 or 0.01) influenced some of the erythrocytes and leukocytes parameters in blood. In Exp.1, mean corpuscular volume (MCV) decreased by CS682 treatments and mean corpuscular hemoglobin (MCH) was highest in Antibiotics treatments. In Exp.2, the level of monocyte (MO) decreased in DCS682-0.10 and 0.20 treatments. The cfu of C. perfringens and S. typhimurium in small intestinal content were highest in Control and lowest in Antibiotics in both experiments. In Exp. 2, DSC682-0.05 and -0.1 treatments were highest and Antibiotic treatment was lowest in Lactobacilli spp. The results of the present layer experiments indicated that supplementation of 0.1~0.2% CS682 or DCS682 may increase eggshell strength, color of eggshell and eggyolk, Haugh unit, and control harmful intestinal microbes.

Identification and Antioxidant Activity of Marine Actinomycetes Streptomyces sp. ACT-1 (해양방선균 Streptomyces sp. ACT-1의 동정 및 항산화 활성)

  • Kim, Man-Chul;Kim, Ju-Sang;Kim, Yun-Beom;Harikrishnan, Ramasamy;Han, Yong-Jae;Heo, Moon-Soo
    • Korean Journal of Microbiology
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    • v.45 no.4
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    • pp.397-403
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    • 2009
  • For the research of the natural antioxidant from marine sources, an antioxidant-producing marine actinomycetes was isolated from sea water in Jeju coastal area. The strain was identified based on 16S rDNA sequencing, the morphology by a method of scanning electron microscopy, physiological and biochemical characteristics and cellular fatty acid analysis. The isolated strain ACT-1 cell size was $0.5\sim1.0{\mu}m$ and gram positive, aerobic, nonmotile, substrate mycelium are red and gray aerial mycelium. 16S rRNA sequence analysis showed that were Gram-positive bacteria grouped on Streptomyces genus. Results of cellular fatty acid analysis showed that major cellular fatty acids were $C_{15:0}$ anteiso (39.33%), $C_{16:1}$ cis 9 (11.96%), $C_{16:0}$ (13.08%) and $C_{17:0}$ anteiso (10.99%). Finally, strain was identified Streptomyces sp. ACT-1. The antioxidant activity of methanol extract from Streptomyces sp. ACT-1 was evaluated by measuring DPPH, hydroxyl, and alkyl radical scavenging activity using an electron spin resonance (ESR) spectrometer. DPPH radical scavenging activity of SBME-1 (Streptomyces broth methanol extract) was 67% at $1,000{\mu}g$/ml. Hydroxyl radical scavenging activity of SBME-1 was 84% at $500{\mu}g$/ml. Alkyl radical scavenging activity of SBME-1 was 71% at $1,000{\mu}g$/ml.

Hue-based Noise-tolerant Corner Detector Robust to Shadows (그림자에 강건한 색상 기반 내잡음성 코너 검출자)

  • 박기현;박은진;최흥문
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.41 no.6
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    • pp.239-245
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    • 2004
  • A hue-based noise-tolerant corner detector is proposed for the exact detection of the real corners in spite of the shadows and random noise. Based on the fact that the hue gradient at the border of the opaque objects' shadow is smaller than the intensity gradient in HSI (hue-saturation-intensity) color space, the effects of shadow are eliminated by introducing the hue-weighted combination of vector gradient to the proposed corner detector. Furthermore, the proposed corner detector is robust to random noise by offsetting the contribution to the corner candidate when the polarities of the color gradients of the pixel pairs are out of phase each other. Results of the experiment show that the proposed corner detector can effectively detect the real corners.

Real-Time Color Gamut Mapping Method Based on the Three-Dimensional Reduced Resolution Look-Up Table (해상도 절감 3차원 룩업 테이블을 이용한 실시간 색역폭 매핑 방법)

  • 한동일
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.41 no.5
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    • pp.225-233
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    • 2004
  • A novel real-time color gamut mapping method is described. The color gamut mapping method that is used for enhancing the color reproduction quality between PC monitor and printer devices is adopted for digital TV display quality enhancement. The high definition digital TV display devices operate at the clock speed of around 70MHz ~ 150MHz and permit several nano seconds for real-time gamut mapping. Thus, the concept of three-dimensional reduced resolution look-up table is introduced for real-time processing. The required hardware can be greatly reduced by look-up table resolution adjustment. The proposed hardware architecture is successfully implemented in FPGA and ASIC and also successfully adopted in digital TV display quality enhancement purposes.

Estimation of spectral Distribution of Illumination using Maximum achromatic Region (최대 무채색 영역을 이용한 광원의 분광분포 추정)

  • Kim, Hui Su;Kim, Yun Tae;Lee, Cheol Hui;Ha, Yeong Ho
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.38 no.4
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    • pp.68-68
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    • 2001
  • 본 논문에서는 단일 영상에 포함된 광원의 분광분포를 추정하는 광원추정 알고리즘을 제안한다. 제안된 광원 추정 방법은 두 단계로 이루어져 있다. 첫째, 변형된 회색계 가정(modified gray world assumption)을 이용하여 부분적으로 광원의 영향을 배제한 후 밝으면서도 무채색에 가까운 최대 무채색 영역을 찾아 그 영역의 표면 분광 반사율을 구한다. 이때 최대 무채색 영역의 표면 분광 반사율은 1269개의 Munsell 색 표본에 대하여 주성분 분석 방법을 이용하여 추정하였다. 둘째, 주어진 Munsell 색 표본과 대표 광원의 조합으로 반사광의 모집단을 만들었다. 다음 최대 무채색 영역의 각 화소와 반사광 모집단과의 색차를 비교하여 최대 무채색 영역과 색차가 가장 적은 반사광 표본을 선택하고 이를 최대 무채색 영역에 대한 반사광의 분광분포로 정의한다. 최종적으로 최저 무채색 영역의 반사광 분광분포를 해당하는 표면 분광반사율로 나누어줌으로써 영상에 포함된 광원의 분광분포를 추정한다. 제안한 알고리듬의 성능을 평가하기 위하여 유색 광원에 조명된 영상에 대한 광원 추정 실험을 하였으며 기존의 방법과 추정된 광원의 분광 분포 비교 및 색차 비교를 통해 그 타당성을 검증하였다.