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Application of extracellular polymeric substances (EPSs)-bioflocculant for recovery of microalgae (미세조류 분리/회수를 위한 세포외 고분자물질 생물 응집제 활용)

  • Choi, Ohkyung;Dong, Dandan;Kim, Jongrack;Maeng, Sung Kyu;Kim, Keugtae;Lee, Jae Woo
    • Journal of Korean Society of Water and Wastewater
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    • v.35 no.1
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    • pp.63-69
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    • 2021
  • Microalgae are primary producers of aquatic ecosystems, securing biodiversity and health of the ecosystem and contributing to reducing the impact of climate change through carbon dioxide fixation. Also, they are useful biomass that can be used as biological resources for producing valuable industrial products. However, harvesting process, which is the separation of microalgal biomass from mixed liquor, is an important bottleneck in use of valorization of microalgae as a bioresource accounting for 20 to 30% of the total production cost. This study investigates the applicability of sewage sludge-derived extracellular polymeric substance (EPS) as bioflucculant for harvesting microalgae. We compared the flocculation characteristics of microalgae using EPSs extracted from sewage sludge by three methods. The flocculation efficiency of microalgae is closely related to the carbohydrate and protein concentrations of EPS. Heat-extracted EPS contains the highest carbohydrate and protein concentrations and can be a best-suited bioflocculant for microalgae recovery with 87.2% flocculation efficiency. Injection of bioflocculant improved the flocculation efficiency of all three different algal strains, Chlorella Vulgaris, Chlamydomonas Asymmetrica, Scenedesmus sp., however the improvement was more significant when it was used for flocculation of Chlamydomonas Asymmetrica with flagella.

Bacteriocin with a Broad Antimicrobial Spectirum, Produced by Bacillus sp. Isolated from Kimchi

  • Mah, Jae-Hyung;Kim, Kwang-Soo;Park, Jong-Hyun;Byun, Myung-Woo;Kim, Young-Bae;Hwang, Han-Joon
    • Journal of Microbiology and Biotechnology
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    • v.11 no.4
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    • pp.577-584
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    • 2001
  • An antimicrobially active bacterium which was identified as Bacillus brevis, was isolated from kimchi. The antimicrobial activity was found against various Gram-positive and Gram-negative bacteria including some pathogens food-spoilage microorganisms, and some yeast strains. The antimicrobial activity was especially strong against Bacillus anthracis and Shigella dysenteriae. The strong activity was observed during an early stationary phase in the culture when incubated at $37^{\circ}C$ with initial medium pH of 6.8. The antimicrobial activity was found to be stable at $90^{\circ}C$ for 30 min and in the pH range of 3-11, and it was insensitive to organic solvents including acetone, acetonitrile, ethanol, and methanol. Analysis of the bacterocin on tricine-sodium dodecyl sulfate-polyacrylamide gel suggested a molecular mass of approximately 4.5-6.0 kDa. The antimicrobial substance was characterized as a bacteriocin, because of its proteinaceous nature and low molecular weight. The bacteriocin could potentially be used as a food preservative, because of its thermostable property and broad antimicrobial spectrum.

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Surface modification and induced ultra high surface hardness by nitrogen ion implantation of low alloy steel

  • Olofinjana, A.O.;Bell, J.M.;Chen, Z.
    • Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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    • 2002.10b
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    • pp.157-158
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    • 2002
  • A surface hardenable low alloy carbon steel was implanted with medium energy (20 - 50KeV) $N_2^+$ ions to produced a modified hardened surface. The implantation conditions were varied and are given in several doses. The surface hardness of treated and untreated steels were measured using depth sensing ultra micro indentation system (UMIS). It is shown that the hardness of nitrogen ion implanted steels varied from 20 to 50GPa depending on the implantation conditions and the doses of implantation. The structure of the modified surfaces was examined by X-ray photoelectron spectroscopy (XPS). It was found that the high hardness on the implanted surfaces was as a result of formation of non-equilibrium nitrides. High-resolution XPS studies indicated that the nitride formers were essentially C and Si from the alloy steel. The result suggests that the ion implantation provided the conditions for a preferential formation of C and Si nitrides. The combination of evidences from nano-indentation and XPS, provided a strong evidence for the existence of $sp^3$ type of bonding in a suspected $(C,Si)_xN_y$ stoichiometry. The formation of ultra hard surface from relatively cheap low alloy steel has significant implication for wear resistance implanted low alloy steels.

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2,4-D Biodegradation Using Microorganism Extracted From Soil (1) (토양미생물에 의한 2, 4-D 분해에 관한 연구 (1))

  • Choung, Youn-kyoo;Lee, Byung chan;Kim, Jin-wook
    • Journal of Korean Society of Water and Wastewater
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    • v.13 no.4
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    • pp.45-53
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    • 1999
  • The microbial organisms named "Pseudomonas sp. LK-14" were isolated from farm land and shallow river sediment, activated, augmented and identified; which were using 2,4-D (2,4-Dichlorophenoxyacetic acid) as a sole carbon source and energy source. 2,4-D removal efficiency of LK-14 with 2,4-D sole carbon source (reactor S) were higher than that of Activated Sludge with 2,4-D sole carbon source (reactor A). Dynamic bioligical reaction kinetic parameters (sole carbon source was 2,4-D) obtained from batch reactor experiments were ${\mu}_{max}$ $0.105hr^{-1}$, $K_{s,24D}$ 15.64mg/L, $K_{i,24D}$ $1.94h^{r-1}$, $Y_{24D}$ 0.39 for LK-14 and ${\mu}_{max}$ $0.008hr^{-1}$, $K_{s,24D}$ 26.95mg/L, $K_{i,24D}$ $1.75hr^{-1}$, $Y_{24D}$ 0.10 for Activated Sludge. Using these parameters, we could predict the behaviors of 2,4-D substrate utilized by LK-14 and Activated Sludge in batch reactors. The kinetic parameters are enable to predict the 2,4-D substrate and microbial population behavior entering into wastewater treatment plants by using unsteady states dynamic simulation modeling technique.

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Evaluation of Fracture Strength and Material Degradation for Weldment of High Temperature Service Steel Using Advanced Small Punch Test

  • Lee, Dong-Hwan
    • Journal of Mechanical Science and Technology
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    • v.18 no.9
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    • pp.1604-1613
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    • 2004
  • This paper presents an effective and reliable evaluation method for fracture strength and material degradation of the micro-structure of high temperature service steel weldment using advanced small punch (ASP) test developed from conventional small punch (CSP) test. For the purpose of the ASP test, a lower die with a minimized ${\Phi}$1.5 mm diameter loading ball and an optimized deformation guide hole of ${\Phi}$3 mm diameter were designed. The behaviors of fracture energy (E$\_$sp/), ductile-brittle transition temperature (DBTT) and material degradation from the ASP test showed a definite dependency on the micro-structure of weldment. Results obtained from ASP test were compared and reviewed with results from CSP test, Charpy impact test, and hardness test. The utility and reliability of the proposed ASP test were verified by investigating fracture strength, behavior of DBTT, and fracture location of each micro-structure of steel weldment for test specimen in ASP test. It was observed that the fracture toughness in the micro-structure of FL+CGHAZ and ICHAZ decreased remarkably with increasing aging time. From studies of all micro-structures, it was observed that FGHAZ microstructure has the most excellent fracture toughness, and it showed absence of material degradation.

Optimal trade-off filters for Noise Robustness, Peak Sharpness and Light Efficiency in the Nonoverlapping Background Noise (배경이 물체에 겹치지 않는 영상에서 잡음에 대한 안정성, 출력의 최대값 크기, 광효율을 고려한 최적 Trade-off 필터)

  • Seong, Yeong-Kyeong;Choi, Tae-Sun
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.37 no.4
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    • pp.56-64
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    • 2000
  • The design of filters for pattern recognition that have optimal trade-off for the criteria of noise robustness, peak sharpness and Homer efficiency, when input scene noise is spatially disjoint (nonoverlapping) with the target, are presented Three different criteria, ie, signal-to-noise ratio, peak-to-output energy ratio and light efficiency are used to design the filter. Computer simulation is made for the various types of noise and parameters to illustrate filter performance for optical pattern recognition. When we compare the new trade-off filter with the original optimal trade-off filter, the performance of trade-off filter for nonoverlapping background is better than that of trade-off filter for overlapping background.

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Carbon nanospheres synthesized via solution combustion method: their application as an anode material and catalyst for hydrogen production

  • Dhand, Vivek;Rao, M. Venkateswer;Prasad, J.S.;Mittal, Garima;Rhee, Kyong Yop;Kim, Hyeon Ju;Jung, Dong Ho
    • Carbon letters
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    • v.15 no.3
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    • pp.198-202
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    • 2014
  • Amorphous agglomerates of carbon nanospheres (CNS) with a diameter range of 10-50 nm were synthesized using the solution combustion method. High-resolution transmission electron microscopy (HRTEM) revealed a densely packed high surface area of $SP^2$-hybridized carbon; however, there were no crystalline structural components, as can be seen from the scanning electron microscopy, HRTEM, X-ray diffraction, Raman spectroscopy, and thermal gravimetric analyses. Electrochemical and thermo catalytic decomposition study results show that the material can be used as a potential electrode candidate for the fabrication of energy storage devices and also for the production of free hydrogen if such devices are used in a fluidized bed reactor loaded with the as-prepared CNS as the catalyst bed.

Simulation of Target Detection using UV and IR Band Signals (UV와 IR 대역 신호를 이용한 표적 신호 검출 시뮬레이션)

  • Du, Gyeong-Su;O, Jeong-Su;Jang, Seong-Gap;Hong, Hyeon-Gi;Seo, Dong-Seon;Choe, Jong-Su
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.38 no.1
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    • pp.79-88
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    • 2001
  • The target such as aircraft operates flares to protect itself from the missile. In general, Infra-red(IR) flares that are hot bodies radiating energy considerably greater than it does, so cause the missile to guide the flare instead of the target. For a precise target tracking in spite of a presence of flares, therefore, the seeker should discriminate the target signal from various clutters including the flare and the background. In this paper, we simulated 2-color rosette scan seeker using IR and UV(UltraViolet) bands. In each wave band, we analyzed the radiant distributions of the target, the flare, and background. These results showed that a simultaneous process in two bands can detect precisely the target.

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Robust Voice Activity Detection in Noisy Environment Using Entropy and Harmonics Detection (엔트로피와 하모닉 검출을 이용한 잡음환경에 강인한 음성검출)

  • Choi, Gab-Keun;Kim, Soon-Hyob
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.47 no.1
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    • pp.169-174
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    • 2010
  • This paper explains end-point detection method for better speech recognition rates. The proposed method determines speech and non-speech region with the entropy and the harmonic detection of speech. The end-point detection using entropy on the speech spectral energy has good performance at the high SNR(SNR 15dB) environments. At the low SNR environment(SNR 0dB), however, the threshold level of speech and noise varies, so the precise end-point detection is difficult. Therefore, this paper introduces the end-point detection methods which uses speech spectral entropy and harmonics. Experiment shows better performance than the conventional entropy methods.

Synthesis LiFePO4- poly(sodium 4-styrenesulfonate) composite cathode material for rechargeable lithium battery by hydrothermal method

  • Hiep, Nguyen Van;Wang, Wan Lin;Jin, En Mei;Gu, Hal-Bon
    • 한국신재생에너지학회:학술대회논문집
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    • 2011.11a
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    • pp.137.2-137.2
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    • 2011
  • Poly (sodium 4-styrenesulfonate) (PSS) is ionomer based on polystyrene that is electrical conductivity and isoviscosity. LiFePO4 has been a promising electrode material however its poor conductivity limits practical application. To enhance the electronic conductivity of LiFePO4, in this study we prepared LiFePO4- PSS composite by the hydrothermal method. LiFePO4 was heated at $170^{\circ}C$ for 12h and then different wt% PSS (0%, 2.91%, 4.75%, 7.36%, 10%) are added to LiFePO4 and milled at 300rpm for 10h. And then the obtained powders were subsequently heated at $500^{\circ}C$ for 1h under argon flow. The cathode electrode were made from mixtures of LiFePO4-PSS: SP-270- PVDF in a weighting ratio 75%: 25%:5%. The electrochemical properties of LiFePO4- PSS/Li batteries were analyzed by cyclic voltammetry and charge/discharge tests. LiFePO4-C/Li battery with 4.75 wt% PSS displays discharge capacity of 128 mAh g-1 at room temperature that is considerably higher than pure LiFePO4/Li battery ( 113.48 mAhg-1).

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