• Title/Summary/Keyword: SP Test

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Isolation of a New Microsporidian sp. (NIK-5hm) forming Spores within the Haemocytes of Silkworm, B. mori L.

  • Selvakumar T.;Nataraju B.;Chandrasekharan K.;Sharma S. D.;Balavenkatasubbaiah M.;Sudhakara Rao P.;Thiagarajan V.;Dandin S. B.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.11 no.1
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    • pp.63-69
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    • 2005
  • While observing silkworm larval samples received from field, microsporidian spores formed within the haemocytes of silkworm haemolymph were observed. The spores of microsporidian sp. were purified and characterized for morphological characters viz., size, shape as well as serological affinity with different Nosema spp. (M$_{11}$ and M$_{12}$). The infectivity of the isolated spores to silkworm was also studied. The microsporidian sp. was found to be highly pathogenic to silkworm, B. mori. The isolated microsporidian sp. was designated as NIK-5hm, which formed ovocylindrical spore in the haemocytes of silkworm and differed in spore size (length, 4.55 $\mu$m & width, 2.10 $\mu$m) and shape from Nosema bombycis (NIK-ls), NIK-2r (Nosema sp. Mysore [3.6 & 2.8 $\mu$m]), NIK-3h (Nosema sp. M$_{11}$ [3.8 & 1.8 $\mu$m]), NIK-4m (Nosema sp. M$_{12}$ [5.0 & 2.1 $\mu$m]) and Lb$_{ms}$ (Nosema sp. in Lamerine breed of silkworm [4.36 & 2.14]). In immonological test (Latex agglutination test), the isolated microsporidian spores did not react with antibody sensitized latex particles of N. bombycis, M$_{11}$, M$_{12}$ and Lb$_{ms}$ and thus are different type of microsporidian sp., parasitic to silkworm, Bombyx mori L.

Degradation Degree Evaluation of Heat Resisting Steel by Electrochemical Technique Part 2 : Effect of Testing Conditions on Evaluation Value of Degradation Degree and Changes of Mechaical Properties (전기화학적 방법에 의한 내열강의 열화도측정 제2보 : 열화도측정치에 미치는 측정조건들의 영향과 기계적성질 변화에 대해서)

  • 정희돈;권영각;장래웅
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.17 no.2
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    • pp.300-312
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    • 1993
  • The material deterioration of service-exposed boiler tube steels in fossil power plant was evaluated by using the electrochemical technique namely, modified electrochemical potentiokinetic reactivation(EPR). It was focused that the passivation of Mo$_{6}$C carbide which governs the mechanical properties of Mo alloyed steels did not occur even in the passivity region of steel in sodium molybdate solution and the reactivation peak current (Ip) observed as the result of non-passivation indicating the precipitation of Mo$_{6}$C carbides. To obtain the optimal test conditions for the field test by using the specially designed electrochemical cell, the effects of scan rate, the surface roughness and the pH of electrolyte on Ip value were also investigated. Furthermore, the change of mechanical properties occurred during the long time exposure at high temperature was evlauated quantitatively by small punch(SP) tests and micro hardness test taking account of the metallurgical changes. It is known that reactivation peak current (Ip) has a good relationship with Larson-Miller Parameter(LMP) which represents the information about material deterioration occurred at high temperature environment. In addition it was possible to estimate the ductile-brittle transition temperature (DBTT) by means of the SP test. The Sp test could be, therefore, suggested as a reliable test method for evaluating the material degradation of boiler tube steels. From the good correaltion between the SP DBTT and Ip values shown in this study, it was knows that the change of mechanical properties could be evaluated non-destructively by measurring only Ip values.ues.

3:1 Bandwidth Switch Module by Using GaAs PH Diode (GaAs PIN Diode를 이용한 3:1 대역폭 스위치 모듈)

  • 정명득;이경학;박동철
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.13 no.5
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    • pp.451-458
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    • 2002
  • Absorptive type SP3T(Single Pole Three Throw) and SP8T switch modules over the 6-18 GHz are designed and fabricated. The epitaxial structure of GaAs PIN diode for switch modules are designed for low loss and high power capability. The maximum input power of SP3T and SP8T switch modules are 2 W and 1 W, respectively. The switching time with driver circuit is less than 130 nsec. The maximum insertion loss of SP3T switch module and SP8T module shows 2.8 dB and 4.2 dB, respectively. The isolation between input port and output port is more than 55 dB. Two switch modules for electronic warfare system have passed the environment tests of the related test items.

토양길항세균 Bacillus sp. KL-3의 대사산물을 이용한 벼도열병균 Pyricularia oryzae의 생물학적방제

  • 김규영;김상달
    • Microbiology and Biotechnology Letters
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    • v.25 no.4
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    • pp.396-402
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    • 1997
  • Biocontrol of plant pathogens provides an alternative means of reducing the incidence of plant diseases without the negative aspects of chemical pesticides. Nowdays, as the resistant fungi about the chemical fungicides have revealed and the concern of environment has increased, the biological control of phytopathogenic fungi by the antagonistic microorganisms is very much indispensable. For the selection of strong antagonistic bacterium for biological control agent of rice leafblast and cucumber gray mold rot, the antifungal strain KL-3 strain was selected among 120 strains isolated from the rhizosphere soils. And the strain was identified to be a species of Bacillus subtilis or closely related strain. In several biochemical and in vitro antibiosis tests, antifungal substances of Bacillus sp. KL-3 were presumed heat stable, micromolecular antibiotic substances. In vivo test and vinyl house field test, the antifungal substances of Bacillus sp. KL-3 represented excellent biocontrol ability aganist Alternaria mali, Phyricularia oryzae, and Alternaria kikuchiana as well as broad spectrum of other fungi. In particular, Bacillus sp. KL-3 strain showed more predominant activity than some chemical fungicides against fungi shown to resist chemcal fungicides.

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Bioelectrochemical Denitrification by Pseudomonas sp. or Anaerobic Bacterial Consortium

  • Park, Doo-Hyun;Park, Yong-Keun
    • Journal of Microbiology and Biotechnology
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    • v.11 no.3
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    • pp.406-411
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    • 2001
  • In a bacterial denitrification test with Pseudomonas sp. and anaerobic consortium, more nitrates and less substrate were consumed but less metabolic nitrite was produced under an anaerobic $H_2$ condition rather than under $N_2$ condition. In a bioelectrochemical denitrification test with the same organisms, the electrochemically reduced neutral red was confirmed to be a substitute electron donor and a reducing power like $H_2$. The biocatalytic activity of membrane-free bacterial extract, membrane fraction, and intact cell for bioelectrochemical denitrification was measured using cyclic voltammetry. When neutral red was used as an electron mediator, the electron transfer from electrode to electron acceptor (nitrate) via neutral red was not observed in the cyclic voltammogram with the membrane-free bacterial extract, but it was confirmed to gradually increase in proportion to the concentration of nitrate in that of the membrane fraction and the intact cell of Pseudomonas sp.

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Screening and effect of antagonists for biological control of plant pathogen (모잘록병의 생물학적 방제를 위한 유효 미생물 선발방법 및 효과)

  • Lee, Baek-Seok;Choe, Seong-Won;Choe, Gi-Hyeon;Lee, Jae-Ho;Kim, Eun-Gi
    • 한국생물공학회:학술대회논문집
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    • 2002.04a
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    • pp.386-389
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    • 2002
  • Antagonistic Bacillus sp. #16 to phytopathogenic fungi were selected based on the growth rate, inhibition rate and surface tension reduction. Based on the 16S rRNA sequences, Bacillus sp. #16 is closely related to the B. subtilis DSM10. In the pot test, Bacillus sp. #16 show the most effective growth inhibition against damping-off disease of cucumber seeding.

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Prevalence and Transmission of Seed-Borne Fungi of Maize Grown in a Farm of Korea

  • Basak, A.B.;Lee, Min-Woong
    • Mycobiology
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    • v.30 no.1
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    • pp.47-50
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    • 2002
  • Seed-borne fungi of some maize cultivars/lines grown during the months from May to September of 2001, collected from Dongguk University farm, Go Young City, IL Sang Gu, Korea were detected by blotter method. In all six fungi namely Alternaria alternata(Fr.) Keissler, Aspergillus niger Van Tiegh, Fusarium moniliforme Sheldon, Fusarium sp., Penicillium sp. and Ustilago zeae Unger. were found to associated with maize seeds. Prevalence of seed-borne fungi also varied. The highest percentages of seed-borne fungi were recorded with Fusarium moniliforme and the lowest in Penicillium sp. Transmission of all seed-borne pathogens from seeds to seedlings were also detected by test tube seedling symptom test. Among the seed-borne fungi, Alternaria alternata, Fusarium moniliforme and Fusarium sp. produced distinct seed rot and seedling infection symptoms. All the transmitted seed-borne fungi might be caused primary source of infection to the maize crop.

Tile Adhesion Strength Change Testing based on Different Concrete Additive Agents (콘크리트 혼화재료 사용에 따른 타일 부착안정성 실험적 연구)

  • Kim, Bum Soo;Seo, Hyun Jae;Choi, Eun Gyu;Lee, Jung Hun;Song, Je Young;Oh, Sang Keun
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2017.11a
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    • pp.165-166
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    • 2017
  • The purpose of this study is to investigate the effect of tile adhesion failure due to weak adhesion with concrete admixture (FA, SP) on walls. The test specimens were divided into four types : (1) OPC 100% (2) OPC 80%+FA 20% (3) OPC 80%+SP 20% (4) OPC 60%+SP 40%, each adhered on a 650 × 650mm wall with 200mm thickness capable of attaching two insulation tiles (300 × 600mm). The tests were carried out on the four types of walls by mortar bedding application method, and after 4 weeks of curing period, adhesion strength test was carried out. The adhesion strength difference was investigated between the concrete wall with added admixture (FA, SP) and general concrete wall.

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Potential of Curvularia sp. DBB2003 as mycoherbicide for monochoria.

  • Kim, Jae-Su;Lee, Han-Young;Jang, Seung-Sik;Chung, Bong-Jin
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.111-111
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    • 2003
  • Several fungal isolates were isolated from diseased monochoria(Monochoria vaginalis, weed of paddy field), which has an resistance to sulfonyl urea(S.U.) herbicide, and were evaluated in the laboratory and greenhouse as potential mycoherbicide. Eight fungi, Alternaria sp., Colletotrichum sp., Curvularia sp., Paenicillium sp and etc. were observed in the isolates. Pathogenicity testing were done on the monochorias in the greenhouse. Monochorias were inoculated with suspensions containing conidia of each isolate at the rates of 1.0 ${\times}$ 10$\^$5/ conidia/ml and 0.1% Tween 80 with hand-gun sprayer. Curvularia sp. and an unidentified fungal isolate caused 90-95% mortality on the monochorias 15∼20 days after inoculation. However the other isolates induced slight symptom of disease on the monochorias. In the early stage of disease development sun-burn appearance was shown at the infected site and the last infected leaves and stems were withered to death. Subsequently the pathogenicity on the rice was evaluated with above two effective isolates. From the test an unidentified isolate showed pathogenicity on the rice but Curvularia sp., named as DBB2003, didn't. Now the mass production and formulation using Curvularia sp. DBB2003 are in progress and the field test will be followed. Combination product with Curvularia sp. DBB2003 and chemical herbicide will be more effect to control the monochoria resisted on S.U. herbicide and need to be further tested.

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Effects on Labor Pain, Length of Delivery Time, and Hemoglobin of Primipara Women treated by San-Yin-Jiao(SP-6) Acupressure 15 minutes and 30 minutes (삼음교(SP6) 지압(15분/30분)이 분만 통증, 분만 소요시간, Hemoglobin에 미치는 영향)

  • Chang, Soon-bok;Park, Young-won;Cho, Jae-sung;Lee, Byung-chul
    • Journal of Acupuncture Research
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    • v.21 no.1
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    • pp.99-110
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    • 2004
  • Objective: The purpose of this study was to examine the different effects on labor pain, length of delivery time, and hemoglobin for primipara women treated by San-Yin-Jiao(SP-6) acupressure of 15 minutes or 30 minutes. Methods: The design of this study was a randomized clinical trial with two experimental groups and one control group, the participants included primiparas who underwent vaginal delivery. Data were collected using a structured questionnaire consisting of general characteristics with a subjective labor pain scale with VA.S, and length of delivery time and hemoglobin were collected in the medical record. Data was analyzed using descriptive statistics, x2- test, t-test, and ANCOVA. Results: There was statistically significant lower labor pain in the group of 30 minutes SP6 acupressure than the control group(t=7.733, p=.007), and there was statistically significant short length of delivery time in the group of 15 minutes SP6 acupressure than the control group(t=-2.230, p=.030). Conclusions: Even though we could not found a consistent effect with the SP6 acupressure, It was found that there was effect on shortening length of delivery time by 15 minutes of SP6 acupressure or lessening labor pain by 30 minutes of SP6 acupressure without risk of blood loss. It is necessary to replicate the study with a larger number of participants to find a consistent result.

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