• Biomolecules & Therapeutics
• /
• 제16권2호
• /
• pp.87-94
• /
• 2008

In view of obtaining potential anticancer compounds, we studied the inhibitory activity and the cytotoxic effects of a candidate compound in cancer cells. The cytotoxic effects of cannabidiol (CBD) in vitro were evaluated in NIH3T3 fibroblasts, B16 melanoma cells, A549 lung cancer cells, MDA-MB-231 breast cancer cells, Lenca kidney cells and SNU-C4 colon cancer cells. The cells were cultured in various concentrations of CBD for 48 h and 25 ${\mu}$M of CBD for 6-36 h. The cells were observed to exhibit inhibitory effects of the cell viability in their growth, and then cytotoxicity was estimated. The inhibitory activity of CBD was increased in all cancer cells and showed especially strong increment in breast cancer cells. The cytotoxicity of CBD increased in a dose- and time-dependent manner with growth inhibition in all cancer cell lines. Also, to assess the membrane toxicity induced by CBD, we investigated lactate dehydrogenase (LDH) release. After treatment with various concentrations of CBD, LDH release rate of cancer cells was accelerated. On the other hand, in the induction of cell death, caspase-3, -8 and -9 activations were detected in cancer cells after treatment with various concentrations of CBD, and CBD effectively induced activity of caspase-3, -8 and -9 in A549 lung cancer cells, MDAMB-231 breast cancer cells and Renca kidney cells. Therefore these results suggest that CBD has a possibility of anticancer agents and anticancer effects against cancer cells by modulation of apoptotic pathway in the range of 5-80 ${\mu}$M concentration.

• 천문학회보
• /
• 제40권1호
• /
• pp.55.1-55.1
• /
• 2015

The first Korean infrared space telescope MIRIS (Milti-purpose InfraRed Imaging System) was successfully launched in November 2013, as the main payload of Korean STSAT-3 (Science and Technology Satellite-3). After initial on-orbit operation for verification, the observations have been made with MIRIS for the fluctuation of Cosmic Infrared Background and the Galactic Plane survey. For the study of near-infrared background, MIRIS completed the survey of large areas (> $10^{\circ}{\times}10^{\circ}$ around the pole regions: the north ecliptic pole (NEP), the north and south Galactic poles (NGP, SGP). We are also continuously and frequently monitoring the NEP region for the instrumental calibration and the zodiacal light study. One the other hand, the Paschen-${\alpha}$ Galactic plane survey has been carried out using two narrow-band filters (at $1.88{\mu}m$ and $1.84+1.92{\mu}m$) of MIRIS. This survey is planning to cover the whole Galactic plane with the latitude of ${\pm}3^{\circ}$, and the longitude regions of $+280^{\circ}<l<360^{\circ}$ and $0^{\circ}<l<+210^{\circ}$ have been completed (~ 80%) by February 2015. The data are still under the stage of reduction and analysis, and we present some preliminary results.

• 복식
• /
• 제57권5호
• /
• pp.123-139
• /
• 2007

Since the late 1980's, the number of research papers published in the Journal of the Korean Society of Costume(JKSC) has increased. The JKSC is usually known for its focus on issues relevant to the aesthetics of dress, fashion design, and the history of Western or Oriental dress. The main goal of this paper is to link the academic importance of the fashion marketing field to the expansion of the journal and society. First, we defined the scope of the fashion marketing field, based on a literature review and general practices of other competitive societies and journals. First, we reviewed the curricula of the fashion marketing field from 49 universities in Korea. Next, we examined the research topics and methodology of 271 papers in the fashion marketing field published in JKSC since its first issue in 1977. By comparing the findings from the curricula and research, we drew conclusions for the fashion marketing field of the journal and society. We found that the approximately 80% of the fashion marketing courses provided at the undergraduate level are related to merchandise planning and selling processes from the company perspective. However, in more than 85% of the research papers, consumer characteristics and decision-making processes were the main focus and were used as key variables. These findings imply that more various methodological approaches are required for the research to enrich the theoretical background which, in turn, can support curricular development in fashion marketing field. The fashion marketing field in JKSC and society should make the most of accumulated knowledge in product design, symbolic aspects of fashion, and the qualitative approach in the research topics of the JKSC and society.

• Journal of Korean Neurosurgical Society
• /
• 제54권4호
• /
• pp.317-322
• /
• 2013

Objective : The surgical approach for recurrent pituitary adenoma after trans-sphenoidal approach (TSA) is challenging. We report the outcomes of the endoscopic TSA for recurrent pituitary adenoma after microscopic TSA. Methods : From February 2010 to February 2013, endoscopic TSA was performed for removal of 30 recurrent pituitary adenomas after microscopic TSA. Twenty-seven (90%) patients had a clinically non-functioning pituitary adenoma. Twenty-four (80%) patients suffered from a visual disturbance related to tumor growth. The clinical features and surgical outcomes were retrospectively analyzed for the ophthalmological, endocrinological, and oncological aspects. Results : The mean tumor volume was 11.7 $cm^3$, and gross total resection was achieved in 50% of patients. The volumetric analysis based on the postoperative MR showed that the mean extent of resection rates were 90%. Vision was improved in 19 (79%) of 24 patients with visual symptoms, and endocrinological cure was achieved in all of three functioning pituitary adenomas; however, the post-operative follow-up endocrinological examination revealed a new endocrinological deficit in one patient. Two patients required antibiotics management for post-operative meningitis. Conclusion : The endoscopic TSA can be an effective treatment option for recurrent pituitary adenoma after microscopic TSA with acceptable outcome.

• 임상간호연구
• /
• 제26권2호
• /
• pp.175-185
• /
• 2020

Purpose: This study was performed to develop a valid and reliable Pediatric Patient Classification System (PPCS). Methods: The study was conducted in a children's hospital which included various ward settings. Content validity was analyzed by Delphi method and to verify intraclass correlation reliability, 7 nurse managers and 29 staff nurses classified 216 patients. To verify construct validity, the staff nurses classified 216 patients according to PPCS comparing differences by age, days of stay, type of stay and medical department. Results: The developed PPCS has 12 categories, 55 nursing activities and 80 criterions. High agreement among nurses (r=.90) suggested substantial reliability. Construct validity was verified by comparing differences in age, days of stay, type of stay and medical department (p<.05). The entire patient group were classified to four groups using PPCS. Conclusion: The findings suggest that PPCS would be a useful tool for estimating nursing demands related to medications and the complexity of pediatric patients.

• Pediatric Gastroenterology, Hepatology & Nutrition
• /
• 제19권1호
• /
• pp.29-37
• /
• 2016

Purpose: The aim of our study was to establish a safe and convenient diagnostic method for acute gastrointestinal (GI) graft-versus-host disease (GVHD) in children by determining the sensitivity and negative predictive values of upper and lower endoscopic biopsies for children suspected of GI GVHD. Methods: Patients suspected of GI GVHD who received endoscopic evaluation within 100 days after stem cell transplantation and endoscopies between January 2012 and March 2014 in Seoul National University Children's Hospital were included in our study. Results: Fifteen patients with a total of 20 endoscopic procedures were included in our study. Sensitivity at the esophagus, stomach, and duodenum were 22.2%, 30.0%, and 80.0%, respectively. Negative predictive values at the esophagus, stomach, and duodenum were 22.2%, 30.0%, and 60.0%, respectively. Overall sensitivity and negative predictive values of upper endoscopic biopsy for GVHD were 77.8% and 50.0%, respectively. Overall sensitivity and negative predictive values of lower endoscopic biopsy for GVHD were 88.9% and 66.7%, respectively. Conclusion: We recommend flexible sigmoidoscopy as a safe and accurate diagnostic tool for GVHD, similar to other studies reported previously. However, if there is no evidence of GVHD on sigmoidoscopy with high index of suspicion of GI bleeding, full colonoscopy and upper endoscopy should be considered.

• 중환자간호학회지
• /
• 제14권2호
• /
• pp.1-11
• /
• 2021

Purpose : The aim of this study was to propose appropriate nurse staffing of adult intensive care units considering patients' nursing care needs according to the Workload Management System for Critical Care Nurses (WMSCN). Methods : In a cross-sectional survey conducted in September 2017, 1,786 patients' WMSCN scores, surveys from 2,145 nurses, and administrative data from 118 units in 41 hospitals were analyzed. The means (standard deviations) of the aforementioned scores and nursing hours per patient day were presented. Nurse-to-patient ratios and nurse-to bed ratios for staffing to meet patients' nursing care needs were calculated. Results : The mean WMSCN scores were 109.50±17.17 in tertiary hospitals and 96.38±19.26 in general hospitals. Nursing hours per patient day were 12.47±2.80 in tertiary hospitals and 11.01±2.45 in general hospitals. Nursing hours per patient day correlated with WMSCN scores. Nurse-to-bed ratios required for the provision of ICU nursing care ranged from 1: 0.36 to 1: 0.48. Conclusion : Our findings provide evidence that current ICU nurse staffing is insufficient for meeting patients' nursing care needs. We suggest adjusting the legal standards for adequate nurse staffing considering these needs.

• Medical Lasers
• /
• 제10권3호
• /
• pp.170-175
• /
• 2021

Background and Objectives Cisplatin is an important chemotherapy drug for the treatment of head and neck cancer. Interstitial laser treatment (ILT) has cosmetic utility and is very important for maintaining the function of the head and neck after cancer treatment. This study examined the synergistic effects of locally injected cisplatin-epigel and high fever induced by an interstitial potassium titanyl phosphate (KTP) laser treatment on a xenografted human Heinz squamous cell carcinoma. Materials and Methods SNU-1041 (107 cells/0.1 ml) cells were xenografted into the back of nude mice by subcutaneous injection. The ILT group (n = 10) was treated with a KTP laser (1 J/mm³) through a cylindrical diffuser tip inserted into the tumor, monitoring the temperature at 43-45℃. In the combined treatment group (n = 10), local hyperthermia was induced by intratumoral injection of 100-200 ㎍ of cisplatin into a collagen-based gel carrier (cisplatin-epigel), which was released slowly four hours before ILT. After four weeks of follow-up, the treated tumors were evaluated for tumor remission and volume change. Results Eight (80%) of the combined group showed complete tumor remission at the four-week follow-up, whereas only three (30%) of the ILT group showed remission (30%) (p < 0.01). Conclusion The current study has shown the synergistic effects of a local cisplatin injection and high fever from ILT on a xenografted human Heinz squamous cell carcinoma.

• 한국수자원학회논문집
• /
• 제40권2호
• /
• pp.147-158
• /
• 2007

본 연구는 기후변화에 따른 수자원 영향평가를 위해 적합한 수문모형을 선택하고 지역기후모형인 SNURCM에서 생성한 모의기상자료로 유출량을 생성하여 모의정확성을 평가하였다. 4개의 월 물수지모형과 두개의 일 유출모형을 이용하여 대청댐 상류유역의 유입량 모의능력을 비교한 결과 abcd모형이 월 물수지모형 중에서는 가장 뛰어났고, 국내에서 널리 사용되고 있는 일 유출모형인 SSARR와 비슷한 모의정확성을 보였다. 다음으로 abcd모형을 금강유역의 12개 소유역에 적용하기 위하여 매개변수 지역화기법을 사용하였다. 9개의 다목적댐에서 구한 매개변수를 미계측유역으로 가정한 4개 다목적댐에 대하여 지역화기법으로 매개변수를 추정하여 유출량을 모의한 결과 모든 지역에서 효율성계수가 최소 87% 이상으로 모의능력이 우수하였다. 마지막으로 금강유역 12개 소유역의 SNURCM모의강수를 실측강수와 비교한 결과 모든 소유역에서의 효율성계수는 60% 이상이었으며, SNURCM 모의강수자료를 abcd모형에 입력하여 생성한 모의유출량을 대청댐 실측유입량과 비교한 결과 효율성계수가 80% 이상으로 기후변화 연구에 활용 가능함을 확인하였다.

• 산업식품공학
• /
• 제15권2호
• /
• pp.130-135
• /
• 2011

오가피는 다양한 생리활성을 가진 약재로 사용되어왔다. 본 연구에서는 오가피 부위별 열수 추출액의 기능적 특징을 규명하기위해 추출부위별 총 폴리페놀 함량, 항산화활성, 아질산염 소거능 및 항암활성을 알아보았다. 오가피의 생리활성 표준물질인 eleutheroside E는 줄기(542.50 $\mu$g/g) >뿌리(343.35 $\mu$g/g) >열매(30.78 $\mu$g/g) 순으로 함유되어 있었고 eleutheroside B는 열매(372.01 $\mu$g/g) >뿌리(289.33 $\mu$g/g) >줄기(125.05 $\mu$g/g) 순으로 많이 함유되어 있었다. 총 폴리페놀은 뿌리(227.21 mg/100 g)와 줄기(131.22 mg/100 g)에 많이 함유되어 있었다. 전자공여능은 줄기에서 79.87%, 뿌리에서 77.27%를 보여 총 폴리페놀 함량과 상관관계가 있었다. 산성 환경에서 nitrosoamine을 생성하는 아질산염에 대한 오가피의 제거 효과는 추출부위와 관계없이 pH 1.2에서 76-81.5%로 높게 나타났다. pH가 높아짐에따라 아질산염제거효과는 대개 소실되었으나 열매 추출액의 경우 pH 6.0에서도 43.1%의 아질산염제거효과를 유지하고 있었다. 오가피 부위별 열수 추출액은 정상세포인 DC2.4의 생육 억제 효과는 관찰되지 않았고 오히려 뿌리추출액의 경우는 DC2.4의 생육을 유의미하게 촉진하는 것으로 밝혀져 세포독성은 없는것으로 판단되었다. 위암 세포주인 SNU-719와 간암세포인 Hep3B에 대해서는 뿌리 추출액과 줄기 추출액에서 20-23%의 억제효과를 보였다. 따라서 오가피 부위별 추출액은 항산화활성, 식육에서의 nitrosoamine 생성 억제 등 다양한 기능성을 가진 식품소재로 활용이 가능 할 것으로 판단되었다.