• 제목/요약/키워드: SNU

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Comparative Study of Autophagy in Oxaliplatin-Sensitive and Resistant SNU-C5 Colon Cancer Cells

  • Boo, Sun-Jin;Piao, Mei Jing;Kang, Kyoung Ah;Zhen, Ao Xuan;Fernando, Pincha Devage Sameera Madushan;Herath, Herath Mudiyanselage Udari Lakmini;Lee, Seung Joo;Song, Seung Eun;Hyun, Jin Won
    • Biomolecules & Therapeutics
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    • 제30권5호
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    • pp.447-454
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    • 2022

  • Few studies have evaluated the role of autophagy in the development of oxaliplatin (OXT) resistance in colon cancer cells. In this study, we compared the role of autophagy between SNU-C5 colon cancer cells and OXT-resistant SNU-C5 (SNU-C5/OXTR) cells. At the same concentration of OXT, the cytotoxicity of OXT or apoptosis was significantly reduced in SNU-C5/OXTR cells compared with that in SNU-C5 cells. Compared with SNU-C5 cells, SNU-C5/OXTR cells exhibited low levels of autophagy. The expression level of important autophagy proteins, such as autophagy-related protein 5 (Atg5), beclin-1, Atg7, microtubule-associated proteins 1A/1B light chain 3B I (LC3-I), and LC3-II, was significantly lower in SNU-C5/OXTR cells than that in SNU-C5 cells. The expression level of the autophagy-essential protein p62 was also lower in SNU-C5/OXTR cells than in SNU-C5 cells. In SNU-C5/OXTR cells, the production of intracellular reactive oxygen species (ROS) was significantly higher than that in SNU-C5 cells, and treatment with the ROS scavenger N-acetylcysteine restored the reduced autophagy levels. Furthermore, the expression of antioxidant-related nuclear factor erythroid 2-related factor 2 transcription factor, heme oxygenase-1, and Cu/Zn superoxide dismutase were also significantly increased in SNU-C5/OXTR cells. These findings suggest that autophagy is significantly reduced in SNU-C5/OXTR cells compared with SNU-C5 cells, which may be related to the production of ROS in OXT-resistant cells.

  • https://doi.org/10.4062/biomolther.2022.028 인용 PDF KSCI

사람 대장암 세포주의 [$^{18}F$fluorodeoxyglucose 섭취의 특징 (Characteristics of [$^{18}F$]fluorodeoxyglucose Uptake in Human Colon Cancer Cells)

  • 김채균;정재민;이명철;고창순;정준기
    • 대한핵의학회지
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    • 제31권3호
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    • pp.381-387
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    • 1997

  • 종양세포는 포도당섭취 및 포도당 대사가 정상세포에 비해 증가된 특징을 가진다. 포도당 유사체인 $^{18}F$ fluorodeoxyglucose(FDG)의 섭취를 이용한 PET 검사가 종양의 진단에 많이 쓰이고 있다. 이 연구에서는 유사한 성질을 가진 사람의 대장암 세포주간의 FDG 섭취량 및 섭취 속도의 차이점을 비교하고, 그 포도당 수송체의 발현의 관련성을 규명하고자 한다. 사람대장암 세포 SNU-C2A, SNU-C4, SNU-C5를 이용하여 FDG 섭취를 측정하였다. 또한 세포의 포도당 섭취에 중요 역할을 하는 포도당 수송체 1(GLUT1)의 발현을 Western blotting으로 비교하였다. $1{\times}10^6/ml$의 대장암 세포에 HEPES-buffered saline에 희석한 $1{\mu}Ci/ml$ FDG를 가하여 $37^{\circ}C$에서 1시간 배양하였을 때 SNU-C2A($16.8{\pm}1.36cpm/{\mu}g$ of protein), SNU-C4($12.3{\pm}5.55$), SNU-C5($61.7{\pm}2.17$) 섭취를 보였다. 시간당 FDG의 섭취는 SNU-C2A($0.29{\pm}0.03cpm/ min/{\mu}g$ of protein), SNU-C4($0.21{\pm}0.09$), SNU C5($1.07{\pm}0.07$)이었으며, 시간이 경과함에 따라 비례하여 증가하였다. Western blotting으로 측정한 GLUT1 은 SNU-C5의 경우 다량 발현되었으나 SNU-C2A와 SNU-C4는 소량 발현되었다. 따라서 SNU-C2A, SNU-C4, SNU-C5 세포는 이들 세포가 비록 유사한 특징을 가졌지만 FDG 섭취량과 섭취 속도 및 GLUT1의 발현이 다르고, 이들 세포의 배가시간(doubling time)은 FDG 섭취와 상관관계가 없었다. 이들 세포의 FDG 섭취와 GLUT1의 발현은 밀접한 상관관계가 있었다.

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칠피(漆皮) 추출물의 담도암 세포주 SNU-1079와 SNU-1196에 대한 항암효과 (Study on Anti-Cancer Effects of Rhus Verniciflua Stokes Extracted with Sterile Distilled Water on Two Cholangiocarcinoma Cell Lines, SNU-1079 and SNU-1196)

  • 정보배;김영철
    • 대한한방내과학회지
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    • 제36권1호
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    • pp.1-12
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    • 2015

  • Objectives : This study was performed to investigate the anti-cancer effects of Rhus verniciflua Stokes (RVS) extracted with sterile distilled water on cholangiocarcinoma cell lines. Materials and Methods : Two cholangiocarcinoma cell lines, SNU-1079 and SNU-1196, were used in this study. Cells were treated with different concentrations of RVS for 24, 48, and 72 hours. Cell count, viability, apoptosis, and mRNA expression of Bax, Bcl-2, Mcl-1, survivin, caspase-3, and cyclin D1 and P21 were determined with an automatic cell counter (ADAM-MC), MTT assay, apoptosis assay (Annexin-V/PI staining), and RT-PCR. Results : All cells treated with RVS showed decreased cell counts in a dose-dependent manner. RVS inhibited proliferation of SNU-1196 in a dose-dependent manner, but SNU-1079 proliferation was inhibited in the long-time culture group in a dose-dependent manner. The proportion of early and late-stage apoptotic cells was increased by RVS in a dose-dependent manner in SNU-1196. In contrast, it was increased significantly in SNU-1079 treated with high-dose RVS. After treatment with RVS, the mRNA expression of Bcl-2 was decreased while Bax was increased in SNU-1079. Cyclin D1 mRNA levels were decreased in SNU-1196 in a dose-dependent manner. P21 expression was increased in all cells after the treatment with RVS. Conclusions : RVS appears to have potential as a therapeutic agent for cholangiocarcinoma.

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