• Toxicological Research
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• 제13권3호
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• pp.247-250
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• 1997

We have studied cytotoxicity of S-nitroso-N-acetyl- N-DL-penicillamine(SNAP), a Nitric oxide (NO)-releasing compound, in RAW264.7 macrophages. SNAP is cytotoxic to RAW264.7 cells in a concentration-dependent manner. PMA(200 nM) stimulated cells to produce superoxide anton radical($O_2^{-\cdot}$) and caused a little loss of RAW264.7 cell viability for 12 hr and diminished the cytotoxicity of SNAP. The mechanism by which PMA can protect cells against NO-mediated cytotoxicity was studied by peroxynitrite-enhanced chemiluminescence method. Observed results suggested that $O_2^{-\cdot}$ produced by PMAstimulated RAW264.7 cells may quench NO released by SNAP and reduce NO, thus attenuating NO-related damages.

• 대한한의학회지
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• 제24권2호
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• pp.159-165
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• 2003

Objectives : Coptidis rhizoma (CR) is an oriental medicine that has been used in many traditional prescriptions against diabetes mellitus in Korea for centuries. Our purpose was to determine the protective effect and its action mechanism of CR on the cytotoxicity of pancreatic -cell line (RINm5F cell). Methods : In this experiment, we used methods such as MTT assay for detection of cytotoxicity, DNA fragmentation assay for detection of apoptotic cell death, LDH activity assay for detection of necrotic cell death, and measurement of $DiOC_{6}$ (3) retention for detection of mitochondrial membrane potential (MMP). Background : Nitric oxide (NO) is believed to playa key role in the process of pancreatic -cell destruction leading to insulin-dependent diabetes mellitus (IDDM). Results : Exposure of RINm5F cells to chemical NO donor such as S-nitroso-N-acetylpenicillamine (SNAP) induced cytotoxic events such as DNA fragmentation and lactate dehydrogenase (LDH) release into medium. However, pretreatment of RINm5F cells with CR extract ($10~50{\mu\textrm{g}}/ml$) for 3 hours prevented SNAP-induced DNA fragmentation and LDH release into medium through the inhibition of MMP disruption. Conclusions : These results suggest that CR may be a candidate for a therapeutic or preventing agent against IDDM.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2003년도 하계학술대회 논문집 Vol.4 No.1
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• pp.392-395
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• 2003

For the first time, the new dual trench gate Emitter Switched Thyristor is proposed for eliminating snap-back effect which leads to a lot of serious problems of device applications. Also, the parasitic thyristor that is inherent in the conventional EST is completely eliminated in the proposed EST structure, allowing higher maximum controllable current densities for ESTs. Moreover, the new dual trench gate allows homogenous current distribution throughout device and preserves the unique feature of the gate controlled current saturation of the thyristor current. The conventional EST exhibits snap-back with the anode voltage and current density 2.73V and $354/{\S}^2$, respectively. But the proposed EST exhibits snap-back with the anode voltage and current density 0.93V and $58A/{\S}^2$, respectively. Saturation current density of the proposed EST at anode voltage 6.11V is $3797A/{\S}^2$. The characteristics of 700V forward blocking of the proposed EST obtained from two dimensional numerical simulations (MEDICI) is described and compared with that of the conventional EST.

• 한국전산구조공학회논문집
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• 제23권3호
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• pp.247-254
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• 2010

유리섬유강화 폴리에스터(GFRP) 복합소재는 가볍고 내구성이 뛰어나 강재, 콘크리트, 나무 등과 같은 기존의 구조 재료들을 대체할 수 있는 재료로 최근 각광 받고 있다. 이러한 복합소재를 활용하기 위해 쉽게 조립할 수 있는 수직결구식 복합소재 데크를 활용한 아치가교 유형을 선행 연구에서 제안하였고 유한요소해석을 통해 검증하였다. 이 논문에서는 선행연구에서 제안된 볼트결합에 의한 복합소재 데크 조립식 아치가교의 안전성 및 사용성을 구조성능시험을 통해 검증하여 문제점을 파악하고 그 문제점을 해결하기 위해 개선된 아치가교 유형을 제안한다.

• Structural Engineering and Mechanics
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• 제89권1호
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• pp.13-22
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• 2024

The nonlinear snap-through buckling of functionally graded (FG) carbon nanotube reinforced composite (CNTRC) curved tubes is analytically investigated in this research. It is assumed that the FG-CNTRC curved tube is supported on a three-parameter nonlinear elastic foundation and is subjected to the uniformly distributed pressure and thermal loads. Properties of the curved nanocomposite tube are distributed across the radius of the pipe and are given by means of a refined rule of mixtures approach. It is also assumed that all thermomechanical properties of the nanocomposite tube are temperature-dependent. The governing equations of the curved tube are obtained using a higher-order shear deformation theory, where the traction free boundary conditions are satisfied on the top and bottom surfaces of the tube. The von Kármán type of geometrical non-linearity is included into the formulation to consider the large deflection in the curved tube. Equations of motion are solved using the two-step perturbation technique for nanocomposite curved tubes which are simply-supported and clamped. Closed-form expressions are provided to estimate the snap-buckling resistance of FG-CNTRC curved pipes rested on nonlinear elastic foundation in thermal environment. Numerical results are given to explore the effects of the distribution pattern and volume fraction of CNTs, thermal field, foundation stiffnesses, and geometrical parameters on the instability of the curved nanocomposite tube.

• 한국산학기술학회논문지
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• 제2권2호
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• pp.1-5
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• 2001

스냅핏은 일종의 결속장치로서 흔히 플라스틱 부품들을 서로 체결하는데 이용되는데, 다른 결속장치에 비해 간편하고. 생산품의 조립단가를 줄일 수 있고, 또한 분리력은 큰 반면에 결합력은 작게 만들 수 있는 장점을 가지고 있어 널리 사용된다. 스냅핏의 설계는 재료에 따른 물성(property) 및 구조적 강성(stiffness)이 설계 초기단계에서 고려되어야 하고, 또한 사출성형공정에서 스냅핏의 성형성도 예측되어야 한다. 그러나 스냅핏의 형상, 치수, 위치 등을 적절히 설계합성(synthesis)하고 적절한 재료를 선택하는 작업은 공정에 관한 종합적인 지식을 지원해 주는 합리적인 설계도구가 제공되지 못했던 이유로 사출 전문가의 오랜 기간 축적된 경험과 지식에만 전적으로 의존하여 왔다. 본 연구에서는 기존의 전문가에 의존해온 스냅핏 설계방법을 개선하기 위해 사출 성형에 의해 제한되어지는 지식을 규칙베이스화하고 재료의 물성과 스냅핏의 형상 및 치수에 따라 스냅핏이 갖는 결합력 분리력 변형하중 및 허용언더컷 등을 출력하는 설계 프로그램을 제안하였다.

• The Korean Journal of Physiology and Pharmacology
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• 제8권3호
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• pp.141-146
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• 2004

Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNARE) proteins, composed of two presynaptic membrane proteins [synaptosomal-associated protein of 25 kDa (SNAP-25) and syntaxin] and a presynaptic vesicular protein [vesicle-associated membrane protein (VAMP)], serve as a core of exocytotic fusion machinery, which can be affected by ischemia. Synaptic protein in core region, striatum and cortex has been shown to alter after focal ischemia, however, little is known in hippocampus. Hippocampus is remote from ischemic core, but it is one of the most vulnerable regions. Using immunohistochemistry, the present study was undertaken to investigate the alteration of expression of SNAP-25, syntaxin, and VAMP in the hippocampus of rats which were subjected to middle cerebral artery occlusion (MCAO) for 2h and allowed to reperfuse. At 2 weeks of reperfusion, the SNAP-25 and syntaxin immunoreactivity was increased in the stratum oriens of the CA1 and the stratum lucidum of the CA3 in the ipsilateral hippocampus. However, VAMP immunoreactivity didn't show significant change. These results demonstrate that the level of the presynatpic plasma membrane proteins (SNAP-25 and syntaxin) in the rat hippocampus is more sensitively affected by focal ischemia than that of the synaptic vesicle protein (VAMP).

• 한국추진공학회지
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• 제9권4호
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• pp.89-95
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• 2005

추력중단장치는 연소관 전방 도움부의 구멍에 설치되며 연소관에 내압 작용 시 쐐기가 강제로 이탈되면 스냅링과 마개가 순차적으로 이탈된다. 이 장치의 변형거동을 이해하기 위하여 수압 구조시험 및 구조해석을 수행하여 그 결과를 비교분석하였다. 추력중단 구멍에 설치되는 분할선의 개수를 대칭적으로 증가시키면 추력중단장치의 기밀성능은 크게 향상된다. 부품간의 마찰계수가 증가하면 추력중단 장치의 이탈압력이 증가하며 패기가 강제 이탈된 상태에서도 스냅링 및 마개의 이탈이 불가능한 임계 마찰계수가 존재한다. 스냅링은 마찰계수가 작으면 반경 방향으로 수축하여 이탈하며 마찰계수가 크면 상당한 소성 변형을 받은 후에도 이탈되지 않는 거동을 보인다.

• Journal of Veterinary Science
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• 제25권3호
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• pp.48.1-48.12
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• 2024

Importance: Early diagnosis of canine pancreatitis is challenging due to non-specific clinical signs. Currently, abdominal ultrasonography and measurement of canine pancreatic lipase (cPL) have been employed for the diagnosis of pancreatitis. Objective: Many qualitative and quantitative commercial cPL tests have been developed and used in veterinary clinics. This study aimed to compare three different methodologies SNAP cPL, Spec cPL, and Vcheck cPL tests to assess the concordance of these assays. Methods: Fifty serum samples were collected from 36 dogs with or without pancreatitis and subjected to SNAP cPL, Spec cPL, and Vcheck cPL tests. Agreement and correlation coefficients were calculated between the test results, and correlations were determined during the management of the patients. Results: The results of the three cPL assays were strongly correlated in 47/50 serum samples (94%). Cohen's kappa analysis between the Spec cPL and Vcheck cPL showed near perfect agreement (κ = 0.960, p < 0.001), SNAP cPL and Vcheck cPL (κ = 0.920, p < 0.001), and Spec cPL and SNAP cPL (κ = 0.880, p < 0.001). The correlation coefficients (r) between data from Spec cPL and Vcheck cPL tests was calculated by Spearman's correlation test (r = 0.958, p < 0.001). Furthermore, the patterns of change in serum cPL concentrations determined using Spec cPL and Vcheck cPL were significantly consistent during the monitoring period in 11 patients. Conclusions and Relevance: Our data illustrated that Spec cPL and Vcheck cPL tests are compatible for clinical use in the diagnosis and monitoring of canine pancreatitis.

• Restorative Dentistry and Endodontics
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• 제16권2호
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• pp.197-212
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• 1991

The purpose of this study was to roughness on the polished surface of visible light composite resins and was to observe the polished surfaces under Scanning Electrom Microscope(ISI DS 130 AKASHI Co. JAPAN). The surface roughness tester(Surfcom 700A Seimtsu profilometer Tokyo, Japan) was used to measure roughness of polished surfaces. In this study, 5 brands of visible of composite resins were examined, Pyrofile light bond Anterior Lite fil anterior Photo clearfil anterior & posterior Palfique light Anterior and posterior Silux Anterior. White point, Silicure point, Super snap Sof-Lex medium disk as cutting instrument, and celluloid matrix were used. The results obtained were as follows. 1. The Celluloid matrix produced the smoothest surfaces. 2. The surfaces made by Soflex medium disk was smoother than the surfaces made by any other polishing instruments. 3. The values of surface roughness made by White point, Silicon point and Super snap were similer. 4. Palfique light (AP) visible light composite resin showed the smoothest surface after polishing with Silicone point, Super snap disk and Soflex mediuem disk in all tested materials.