Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Superoxide Formation and Cytotoxicity of RAW264.7 Macrophages Induced by Nitric Oxide

  • Lee, Hong (Department of chemistry, Wonkwang university. Iksan) ;
  • Pae, Hyun-Ock (Department of chemistry, Wonkwang university. Iksan) ;
  • Jun, Chang-Duk (Department of microbiology and immunology, Wonkwang university school of medicine, Iksan. Korea) ;
  • Yoo, Ji-Chang (Department of microbiology and immunology, Wonkwang university school of medicine, Iksan. Korea) ;
  • Park, Rae-Kil (Department of microbiology and immunology, Wonkwang university school of medicine, Iksan. Korea) ;
  • Chung, Hun-Taeg (Department of microbiology and immunology, Wonkwang university school of medicine, Iksan. Korea)
  • Published : 1997.09.01
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Abstract

We have studied cytotoxicity of S-nitroso-N-acetyl- N-DL-penicillamine(SNAP), a Nitric oxide (NO)-releasing compound, in RAW264.7 macrophages. SNAP is cytotoxic to RAW264.7 cells in a concentration-dependent manner. PMA(200 nM) stimulated cells to produce superoxide anton radical($O_2^{-\cdot}$) and caused a little loss of RAW264.7 cell viability for 12 hr and diminished the cytotoxicity of SNAP. The mechanism by which PMA can protect cells against NO-mediated cytotoxicity was studied by peroxynitrite-enhanced chemiluminescence method. Observed results suggested that $O_2^{-\cdot}$ produced by PMAstimulated RAW264.7 cells may quench NO released by SNAP and reduce NO, thus attenuating NO-related damages.

Keywords

References

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