• KSBB Journal
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• v.22 no.4
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• pp.179-184
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• 2007

This study introduces the characteristics and some applications of repetitive polypeptides, especially to the biomaterial, tissue engineering scaffolds, drug delivery system, and DNA separation systems. Since some fibrous proteins, which consist of repeating peptide monomers, have been reported that their physical properties are changed dramatically by means of temperature alteration or pH shifting. For that reason, fibrous protein-mimetic polypeptides, which are produced by the recombinant technology, can be applied to the diverse biological fields. Repetitive polypeptides can also be used in the bioseparation area such as DNA sequencing, because they make DNA separation possible in free-solution electrophoresis by conjugating DNA fragments to them. Moreover, artificial synthesis of repetitive polypeptides helps to demonstrate the correlations between mechanical properties and structures of natural protein polymer, which have been proven that repetitive domains are affected by the sequence of the repeating domains and the number of repeating subunits. Repetitive polypeptides can be biologically synthesized using some special cloning methods, which are represented here. Recursive directional ligation (RDL) and controlled cloning method (CCM) have been proposed as excellent cloning methods in that we can control the number of repetition in the multimerization of polypeptides and the components of repetitive polypeptides by either method.

• Atmosphere
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• v.24 no.2
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• pp.131-140
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• 2014

The "Barents Oscillation (BO)", first designated by Paul Skeie (2000), is an anomalous recurring atmospheric circulation pattern of high relevance for the climate of the Nordic Seas and Siberia, which is defined as the second Emperical Orthogonal Function (EOF) of monthly winter sea level pressure (SLP) anomalies, where the leading EOF is the Arctic Oscillation (AO). BO, however, did not attracted much interest. In recent two decades, variability of BO tends to increase. In this study, we analyzed the spatio-temporal structures of Atmospheric internal modes such as Arctic Oscillation (AO) and Barents Oscillation (BO) and examined how these are related with Arctic warming in recent decade. We identified various aspects of BO, not dealt in Skeie (2000), such as upper-level circulation and surface characteristics for extended period including recent decade and examined link with other surface variables such as sea-ice and sea surface temperature. From the results, it was shown that the BO showed more regionally confined spatial pattern compared to AO and has intensified during recent decade. The regional dipolelar structure centered at Barents sea and Siberia was revealed in both sea-level pressure and 500 hPa geopotential height. Also, BO showed a stronger link (correlation) with sea-ice and sea surface temperature especially over Barents-Kara seas suggesting it is playing an important role for recent Arctic amplification. BO also showed high correlation with Ural Blocking Index (UBI), which measures seasonal activity of Ural blocking. Since Ural blocking is known as a major component of Eurasian winter monsoon and can be linked to extreme weathers, we suggest deeper understanding of BO can provide a missing link between recent Arctic amplification and increase in extreme weathers in midlatitude in recent decades.

• The Transactions of the Korean Institute of Power Electronics
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• v.3 no.1
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• pp.1-7
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• 1998

Due to the increase in capacity of auxiliary machinery in power plant, the importance of energy saving has been greatly emphasized. If the speed of fans or pumps is controlled in accordance with the variation of load, large electric energy can be saved. Large capacity inverter, 2MVA GTO inverter, has been developed by operating two of 1MVA unit inverters in parallel. The parallel operation of the unit inverter is accomplished through two output transformers of which the secondary windings are connected in series. The system is composed of one control cubicle, one rectifier cubicle and 2 unit inverter cubicles. This inverter system was applied to the sea water lift pump(SLP) driven by a 6.6KV 1500KW induction motor in Seo-Inchon power plant to save the electric energy. The parallel operation of inverters by 180 degrees apart in switching frequency helps to reduce the harmonic components.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.30 no.1A
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• pp.10-27
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• 2005

In this paper, we propose a high level design architecture of WCDMA(UMTS) base station modem and synchronization algorithms applied to the proposed architecture. Also analysis of each synchronization algorithm and performance evaluation of fixed point designed modem are shown. Since the target system is base station modem, each synchronization algorithm is designed for its stable operation. To minimize implementation complexity, optimum fixed point design for best operation of synchronization algorithms is performed. We performed symbol level link simulation with fixed point designed modem simulator for data rate of 12.2kbps, 64kbps, 144kbps, and 384kbps. We compared performance results to the minimum requirements specified in 3GPP TS 25.104(Release 5). Extensive computer simulation shows that the proposed modem architecture has stable operation and outperform the minimum requirement by 2 dB. The proposed modem architecture has been applied in the implementation of WCDMA reverse link receiver modem chip successfully.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.4
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• pp.470-475
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• 1999

This study was conducted to evaluate the utilization of animal by-product mixture (ABPM) as a dietary animal protein source of fish meal replacer, and to determine the effect of dietary chromic oxide in growing rainbow trout, Oncorhynchus mykiss. ABPM is a mixture of five anmial by-products such as meat and bone meal (MBM) feather meal (FM), squid live, powder(SLP), poultry by-product (PBP) and blood- meal (BM) at a specific weight based ratio. Diet 1 and 2 were formulated on a isonitrogenous and a isocaloric basis of $46.5\%$ crude protein and 16.7 KJ/g diet; diet 1 (WFM 100), $100\%$ of the animal protein source came from white fish meal; diet 2 (ABPM 40), $60\%$ WFM+$40\%$ ABPM as the animal protein source; diet 3 (-Cr) commercial diet without chromic oxide; diet 4 (+Cr), commercial diet with chromic oxide. After eight weeks of feeding trials, fish fed diet 2 had a significantly lower body weight gain (WG) and feed efficiency (FE) than that of fish fed the other diets (P<0.05). When comparing diet 3 with diet 4, no significant differences were found in WG and FE (P>0.05). There were no significant differences on condition factor, hematocrit level, serum phosphorus, bone phosphorus, whole body phosphorus, and bone ash among fish from all four diet groups. Fish fed diet 4 had a significantly higher whole body lipid than that of fish fed the other diets (P<0.05), These results indicated that ABPM could be used less than $40\%$ in growing rainbow trout with a sufficient period of acclimation, In addition, the $0.5\%$ of chromic oxide can be used to determine the apparent digestibility of the nutrients in the feed without any adverse effects on growth and body composition.

• Journal of the Korean Society of Hazard Mitigation
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• v.10 no.1
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• pp.89-102
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• 2010

Future uncertainty on water demand caused by future climate condition and water consumption leads a difficulty to determine the reservoir operation rule for supplying sufficient water to users. It is, thus, important to operate reservoirs not only for distributing enough water to users using the limited water resources but also for preventing floods and drought under the unknown future condition. In this study, the reservoir storage is determined in the first stage when future condition is unknown, and then, water distribution to users and river stream is optimized using the available water resources from the first stage decision using 2-stage stochastic linear programming (2-SLP). The objective function is to minimize the difference between target and actual water storage in reservoirs and the water shortage in users and river stream. Hedging rule defined by a precaution against severe drought by restricting outflow when reservoir storage decreases below a target, is also applied in the reservoir operation rule for improving the model applicability to the real system. The developed model is applied in a system with five reservoirs in the Han River basin, Korea to optimize the multi-reservoir system under various future water demand scenarios. Three multi-purposed dams - Chungju, Hoengseong, and Soyanggang - are considered in the model. Gwangdong and Hwacheon dams are also considered in the system due to the large capacity of the reservoirs, but they are primarily for water supply and power generation, respectively. As a result, the water demand of users and river stream are satisfied in most cases. The reservoirs are operated successfully to store enough water during the wet season for preparing the coming drought and also for reducing downstream flood risk. The developed model can provide an effective guideline of multi-reservoir operation rules in the basin.

• Journal of Korean Association for Spatial Structures
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• v.8 no.4
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• pp.47-55
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• 2008

This paper deals with basic theories and some numerical results on structural optimization for geodesic dome. First of all, the space efficiency of geodesic dome is investigated by using the ratio of icosahedron's surface area to the internal volume enclosed by it. The procedure how to create the geodesic dome is also provided in systematic way and implemented and utilized into the design optimization code ISADO-OPT. The mathematical programming technique is introduced to find out the optimum pattern of member size of geodesic dome against a point load. In this study, total weight of structure is considered as the objective function to be minimized and the displacement occurred at loading point and member stresses of geodesic dome are used as the constraint functions. The finite difference method is used to calculate the design sensitivity of objective function with respect to design variables. The SLP, SQP and MFDM available in the optimizer DoT is used to search optimum member size patterns of geodesic dome. It is found to be that the optimum member size pattern can be efficiently obtained by using the proposed design optimization technique and numerical results can be used as benchmark test as a basic reference solution for design optimization of dome structures.

• The Journal of the Korea Contents Association
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• v.11 no.9
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• pp.407-413
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• 2011

Distinguishing aphasia in stroke patients and observing the subtle linguistic characteristics associated with it primarily requires the use of instruments that provide reliable assessment results. Additionally, examiners should be fully aware of how to use those instruments. This study examined 46 stroke patients for aphasia and assessed the reliability of their diagnoses according to examiners whose medical fields were different from each other. Furthermore, a comparison was made between the reliability before training and that after training. To this end, 46 stroke patients were tested for aphasia and in terms of their speech disorder degree by 3 groups, each of which consisted of 12 professionals (3 SLP, 3 neurologist, and 3 nurse). In the result, a rating of 'acceptable' was given for speech intelligibility tasks and the voice quality of /ah-/ prolongation, and other sub-tests were marked as 'good-excellent' by the experts with different areas of medical expertise. For the tasks marked as 'acceptable', the subjects were video-trained for 3 weeks and the differences were compared before and after their training. Consequently, the differences in the examiners' ratings in the speech intelligibility tasks showed a significant decrease and the accuracy of their voice quality ratings showed a significant increase. In the result of research on the correlation between the accuracy of the sub-test ratings and the amount of clinic experience, speech therapists developed more accuracy in rating a picture description task and a speech intelligibility task as their experience accumulated. Meanwhile, doctors and nurses showed more accurate ratings in picture description tasks with greater clinical experience. The results of this study suggest that assessing the neurologic-communicative disorders of stroke patients requires ongoing training and experience, especially for speech disorders. It was also found that the rating reliability in this case could be improved by training.

• The Plant Pathology Journal
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• v.36 no.4
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• pp.323-334
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• 2020

Lysin motif (LysM) proteins are reported to be necessary for the virulence and immune response suppression in many herbaceous plant pathogens, while far less is documented in woody plant pathogens. In this study, we preliminarily characterized the molecular function of a LysM protein LtLysM1 in woody plant pathogen Lasiodiplodia theobromae. Transcriptional profiles revealed that LtLysM1 is highly expressed at infectious stages, especially at 36 and 48 hours post inoculation. Amino acid sequence analyses revealed that LtLysM1 was a putative glycoprotein with 10 predicted N-glycosylation sites and one LysM domain. Pathogenicity tests showed that overexpressed transformants of LtLysM1 displayed increased virulence on grapevine shoots in comparison with that of wild type CSS-01s, and RNAi transformants of LtLysM1 exhibited significantly decreased lesion length when compared with that of wild type CSS-01s. Moreover, LtLysM1 was confirmed to be a secreted protein by a yeast signal peptide trap assay. Transient expression in Nicotiana benthamiana together with protein immunoblotting confirmed that LtLysM1 was an N-glycosylated protein. In contrast to previously reported LysM protein Slp1 and OsCEBiP, LtLysM1 molecule did not interact with itself based on yeast two hybrid and co-immunoprecipitation assays. These results indicate that LtLysM1 is a secreted protein and functions as a critical virulence factor during the disease symptom development in woody plants.

• Journal of Aquaculture
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• v.10 no.2
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• pp.143-151
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• 1997

A six week feeding trial was conducted to determine the amount of fish meal analog (FMA) that can be replacing fish meal protein (FM) in Korean Rockfish. Seven experimental diets were formulated on isonitrogenous 52% crude protein and isocaloric basic 16.8KJ/g diet. Also, foreign commercial fish meal analog (CFMA) and attractants (ATT) were tested in this experiment. Percentage of the graded level of replacement of FM by FMA/CFMA on the basis of crude protein were as following : Diet 1, 100%FM ; Diet by 2, 60%FM : 40% CFMA ; Diet 3, 60%FM : 40$ CFMA+ATT ; Diet 4, 80%FM : 20%FMA ; Diet 5, 80%FM : 20% FMA+ATT ; Diet 6, 60%FM : 40%FMA+ATT ; Diet 7, 40%FM : 60% FMA+ATT. The FMA was made by mixing six animal protein source such as th blood meal, squid liver powder, meat and bond meal, leather meal, feather meal, poultry by-product and 3 essential amino acids (Met, Lys, Ile). Weight gain, feed efficiency, specific growth rate and protein efficiency ratio of fish fed diets 4, 5 and 6 were not significantly different (P>0.05) from those of fish fed the control (100% FM), while those of fish fed diets 2 and 3 were significantly lower (P<0.05) than those of fish fed the diet 6. There was no significant ATT effects in this study (P>0.05). Significant differences were found in hepatosomatic index, hemoglobin and condition factor. Therefore, these, results indicated that FMA can be used up to 40% as a substitute of fish meal protein in Korean Rockfish diets.