• Journal of Acupuncture Research
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• v.20 no.5
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• pp.50-62
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• 2003

Objective: Bee Venom(BV) has been used for various kinds of inflammatory or painful conditions in Oriental Medicine clinics, and there publishes reports on its therapeutic effects and the probable mechanism of those therapeutic effects, where CDs and cytokines plays important role. This study investigated the influences of bee venom on the expressions of CDs and cytokines of HMC cell line Methods: In this study we analysed the expression profile of HMC cell line treated with BV of 10-2ug/ml in relation to that of HMC cell line treated with vehicle by way of CD/cytokine microarray hybridization with 342 genes on it. Results: There were no upregulated genes by more than 3 fold, while there showed some downregulated genes by less than 1/3 fold as follows: colony stimulating factor 2, CD122, IL-7, CD112, TNF-alpha, CD138, CD166, TGFbetaR2, CD42b, CD62L, CD111, interleukin 10 receptor alpha, colony stimulating factor 1(macrophage), CD38 antigen(p45), CD121a, CD33 antigen(gp67), colony stimulating factor 1 receptor, B cell linker protein (SLP65) mRNA, CD94, alanyl(membrane) aminopeptidase, immunoglobulin(CD79A) binding protein 1, CD205, CD241, CD207, CDw121b, integrin alpha L(CD11a), integrin beta 1(CD29), CD91, CD42b. Conclusions: Bee venom treatment induced downregulation of some CDs or cytokines including $TNF-{\alpha}$. IL-1R with its possible implication in an antiinflammatory action of BV. Further research on expression profile changes induced by BV treatment is expected.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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• v.17 no.1
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• pp.43-48
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• 2006

Background and Objectives: Countertenors who can produce higher vocal pitch like female classical singer's voice and use both modal and falsetto register. This study was conducted to study phonatory characteristics between modal and falsetto register of the countertenor. Materials and Methods: A male countertenor who had 8 years of experience was examined using a videostroboscopy and his voice was analyzed using aerodynamic measures; fundamental frequency(F0), Mean air flow rate(MFR), intensity(SLP), subglottal air pressure(Psub) with phonatory function analyzer(Nagashima) and acoustic measures; jitter, shimmer, HNR, closed quotient(CQ) using a Electro-glottography(EGG) of Lx. Speech Studio(Laryngoscope, Ltd, UK) and voice range profile of CSL(Kay elemetrics). Results: In the stroboscopy finding, the longitudinal length of vocal folds was increased at the falsetto register and the upper margin of vocal folds vibrated with incomplete closure of true vocal folds. In aerodynamic analysis, intensity was same at the modal and falsetto register. However, MFR, Psub, MPT were higher at the falsetto register. In the electroglottographic analysis, closed quotient(CQ) at the modal register was high and also much higher at the high-pitch falsetto than at the loud falsetto. In the VRP, intensity was similar though F0 was different between modal and falsetto register. Conclusion: It implied that countertenor could produce powerful voice quality by increasing of respiratory pressure and respiratory volume though glottal closure was incomplete. In addition, no change of EGG waveform, similar voice range with alto was observed.

• Journal of Advanced Navigation Technology
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• v.18 no.5
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• pp.508-512
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• 2014

This paper suggests the control system and algorithm for auto and manual control of the streetlight using RF system. There are two control system in this auto control algorithm. One is group control, the other individual control. In case of group control, if a car is detected by the object detecting sensor of the system installed on the streetlight, it will turn on the light per group by transmitting the RF signal. The streetlight turns on separately when it detects people or a car parked on the shoulder in accordance with the individual control. Also, there is manual control algorithm that manager can check surrounding environment and condition of the streetlight by RF signal and various sensors. So, not only the proposed system reduce meaningless energy consumption, but also it offers convenience regarding maintenance and control of the streetlight.

• Transactions of the Korean Society of Automotive Engineers
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• v.12 no.3
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• pp.101-108
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• 2004

A FEM-based efficient method is developed for the shape optimization of 2-D structures. The combined SLP and Simplex method are coupled with finite element analysis. Selected set of master nodes on the design boundaries are employed as design variables and assigned to move towards their normal directions. The other nodes along the design boundaries are grouped into the master node. By interpolating the repositioned master nodes, the B-spline curves are formed so that the rest mid-nodes efficiently settle down on the B-spline curves. Mesh smoothing scheme is also applied for the nodes on the design boundary to maintain most finite elements in good quality. Finally, a numerical implementation of optimum design of an automobile torque converter piston subjected to pressure and centrifugal loads is presented. The results shows additional weight up to 13% may be saved after the shape optimization.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.31 no.8
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• pp.27-35
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• 2003

Geometric modeling tool and analysis tool of shell surface have been developed in the different environments and purposes. Thus they cannot be naturally fitted to each other for the integrated design and analysis. In the present study, an integrated framework of geometric modeling, analysis， and design optimization is proposed. It is based on the common representation of B-spline surface patch. In the analysis module, a geometrically-exact shell finite element is implemented. In shape optimization module, control points of the surface are selected as design variables. For the computation of shape sensitivities, semi-analytical method is used. Sequential linear programming(SLP) is adopted for the shape optimization of surfaces. The developed integrated framework should serve as a powerful tool for the geometric modeling, analysis, and shape design of surfaces.

• Microbiology and Biotechnology Letters
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• v.29 no.1
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• pp.1-11
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• 2001

Lactic acid bacteria (LAB) are widely used for various food fermentation. With the recent advances in modern biotechnology, a variety of bio-products with the high economic values have been produced using microorganisms. For molecular cloning and expression studies on the gene of interest, E. coli has been widely used mainly because vector systems are fully developed. Most plasmid vectors currently used for E, coli carry antibiotic-resistant markers. As it is generally believed that the antibiotic resistance markers are potentially transferred to other bacteria, application of the plasmid vectors carrying antibiotic resistance genes as selection markers should be avoided, especially for human consump-tion. By contrast, as LAB have some desirable traits such that the they are GRAS(generally recognized as safe), able to secrete gene products out of cell, and their low protease activities, they are regarded as an ideal organism for the genetic manipulation, including cloning and expression of homologous and heterologous genes. However, the vec-tor systems established for LAB are stil insufficient to over-produce gene products, stably, limiting the use of these organisms for industrial applications. For a past decade, the two popular plasmid vectors, pAM$\beta$1 of Streptococcus faecalis and pGK12 theB. subtilis-E. coli shuttle vector derived from pWV01 of Lactococcus lactis ssp. cremoris wg 2, were most widely used to construct efficient chimeric vectors to be stably maintained in many industrial strains of LAB. Currently, non-antibiotic markers such as nisin resistance($Nis^{r}$ ) are explored for selecting recombi-nant clone. In addition, a gene encoding S-layer protein, slp/A, on bacterial cell wall was successfully recombined with the proper LAB vectors LAB vectors for excretion of the heterologous gene product from LAB Many food-grade host vec-tor systems were successfully developed, which allowed stable integration of multiple plasmid copies in the vec-mosome of LAB. More recently, an integration vector system based on the site-specific integration apparatus of temperate lactococcal bacteriophage, containing the integrase gene(int) and phage attachment site(attP), was pub-lished. In conclusion, when various vector system, which are maintain stably and expressed strongly in LAB, are developed, lost of such food products as enzymes, pharmaceuticals, bioactive food ingredients for human consump-tion would be produced at a full scale in LAB.

• Proceeding of KASS Symposium
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• 2008.05a
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• pp.101-106
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• 2008

This paper describes a comparative study of genetic algorithm and mathematical programming technique applied in the design optimization of geodesic dome. In particular, the genetic algorithm adopted in this study uses the so-called re-birthing technique together with the standard GA operations such as fitness, selection, crossover and mutation to accelerate the searching process. The finite difference method is used to calculate the design sensitivity required in mathematical programming techniques and three different techniques such as sequential linear programming (SLP), sequential quadratic programming(SQP) and modified feasible direction method(MFDM) are consistently used in the design optimization of geodesic dome. The optimum member sizes of geodesic dome against several external loads is evaluated by the codes $ISADO-GA{\alpha}$ and ISADO-OPT. From a numerical example, we found that both optimization techniques such as GA and mathematical programming technique are very effective to calculate the optimum member sizes of three dimensional discrete structures and it can provide a very useful information on the existing structural system and it also has a great potential to produce new structural system for large spatial structures.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.5 no.1
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• pp.1-12
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• 1985

This study is conserned for the optimum design of reinforced concrete slab-beam-column structures with multi-storys and multi-bays by Direct Method. Flexural and shear strength, sectional size, and steel ratio etc., were considered as the design-constraints and the cost function was taken as to objective function. They became high degree nonlinear problems. Using SLP as an analytical method of nonlinear optimal problems, an optimal algorithm was developed in this study and the algorithm was applied to the optimization of reinforced concrete structure system of 5 storys. The result converged to a optimal solution with 3 to 5 iterations, and proved that economical design could be possible when compared with conventional designs.

• International journal of advanced smart convergence
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• v.9 no.2
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• pp.8-19
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• 2020

We see an energy fucused smart city evolution of the UK along with the project of "Smart London Plan (SLP)." A theoretical logic of business model innovation has been discussed and a research framework of evolving energy focused smart city is formulated. The starting point is the silo system. In the second stage, the private investment in smart meters establishes a basement for next stages. As results, the UK's smart energy sector has evolved from smart meter installation through smart grid to new business models such as water-energy nexus and microgrid. Before smart meter installation of the government, the electricity system was centralized. However, after consumer engagement plan has been set to make them understand benefits that they can secure through smart meters, the customer behavior has been changed. The data analytics firm enables greater understanding of consumer behavior and it helps energy industry to be smart via controlling, securing and using that data to improve the energy system. In the third stage, distribution network operators (DNOs)' access to smart meter data has been allowed and the segmentation starts. In the fourth stage, with collaboration of Ofwat and Ofgem, it is possible to eliminate unnecessary duplication of works and reduce interest conflict between water and electricity. In the fifth stage, smart meter and grid has been integrated as an "adaptive" system and a transition from DNO to DSO is accomplished for the integrated operation. Microgrid is a prototype for an "adaptive" smart grid. Previous steps enable London to accomplish a platform leadership to support the increasing electrification of the heating and transport sector and smart home.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.9 no.3
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• pp.57-64
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• 1989

This study presents a reliability-based optimum design of reinforced concrete frames, in which the AFOSM and SOSM methods are applied for the evaluation of the failure probabilities, and the sequential linear programming method is used as a practical approach to the system optimization. One-story two-bay reinforced concrete frame is chosen for the numerical illustration of the proposed reliability-based optimum design. As a result, it is found that the proposed procedure for the reliability-based optimization of RC frames could provide the accurate estimation of the optimal level of safety, and appears applicable to real structures with reasonable complexity. It is shown in the paper that the probability distributions of the basic random variables and the uncertainties of the applied loadings and material strengths may have great effect on the optimum design, but the AFOSM and SOSM methods do not show significant discrepancy in the optimum design results, but the former appears more realistic and time saving than the latter for this specific study.