• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2006년도 춘계 학술발표회 논문집
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• pp.662-669
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• 2006

PEM(Prestressed Earth Method) is a method to minimize lateral movements of the ground generated by progressive excavation and increases shear strength by applying prestresses to the end of earth bolt equipped with a P.C. panel after earth bolt is set up under the in-situ ground. In case of PEM, there are noticeable advantages. First of all, PEM maximizes the utility of the ground because PEM needs less volume of backfill and cutting than other general walls. Second, it's an environmental method possible to garden on the banquette. In this study, the behavioral characteristics of PEM are analyzed and compared with soil nailing system through the measured data of PEM and numerical method using SMAP-2D program and also an increased stability of PEM is evaluated by increasing prestress of earth bolts through the numerical analysis using Slide (ver. 4.0) program.

• The Plant Pathology Journal
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• 제19권1호
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• pp.34-42
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• 2003

The ascomycete Magnaporthe grisea is a pathogen of rice blast and is known to form specialized infection structures called appressoria for successful infection into host cells. To understand the molecular mechanism underlying infection process, appressorium-related genes were identified through global approaches including EST sequencing, differential hybridization, and sup-pression subtractive hybridization. EST database was generated on >2,000 cDNA clones randomly selected from appressorium stage cDNA library. Large number of ESTs showed homology to known proteins possibly involved in infection-related cellular development (attachment, germination, appressorium formation, and colonization) of rice blast fungus. The 1051 ESTs showing significant homology to known genes were assigned to 11 functional categories. Differential hybridization and suppression subtractive hybridization were applied to identify genes showing an appressorium stage specific expression pattern. A number of genes were selected as up-regulated during appressorium formation compared with the vegetative growing stage. Clones from various cDNA libraries constructed in different developmental stages were arrayed on slide glass for further expression profiling study. functional characterization of genes identified from these global approaches may lead to a better understand-ing of the infection process of this devastating plant disease, and the development of novel ways to protect host plant.

• 한국재료학회지
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• 제13권8호
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• pp.550-554
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• 2003

Current nanogold cluster synthesized by chemical routine with 11 or 55 atoms of gold has been widely used for immuno chemistry probe as a form of nanocluster conjugated with biomolecules. It would be an undeveloped region that the 1 nm size of nanogold could be made by materials engineering processing. Therefore, objective of this study is to minimize the size of gold nanocluster as a function of operating temperature and chamber pressure in inert gas condensation (IGC) processing. Evaporation temperature was controlled by input current from 50 A to 65 A. Chamber pressure was controlled by argon gas with a range of 0.05 to 2 torr. The gold nanocluster by IGC was evaluated by X-ray diffraction (XRD) and transmission electron microscopy (TEM). The gold nanocluster for TEM analysis was directly sampled with special in-situ method during the processing. Atomic force microscopy (AFM) was used to observe 3-D nanogold layer surfaces on a slide glass for the following biomolecule conjugation step. The size of gold nanoclusters had a close relationship with the processing condition such as evaporation temperature and chamber pressure. The approximately 1 nm size of nanogold was obtained at the processing condition for 1 torr at $1124 ^{\circ}C$.

• 종양간호연구
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• 제2권1호
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• pp.50-60
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• 2002

Breast self-exam is easiest, safe and cost effective to be recommended as an important method for early detection of breast cancer. This experimental research with non-equal control design was to develop the efficacy expectation program for breast self-exam based on Bandura's self-efficacy information source. The study objects selected from two local churches in Busan. Twenty from S church were selected as a study group, twenty from D church as a control group. Efficacy expectation promoting program was based on Bandura's efficacy promoting source and was two hour lecture including slide, video tape, demonstration, pamphlet. After this, there were individual interviews with them and consultations through telephone f or verbal persuasion on the weekly basis between the first week and the fifth week. The effect from the experiment were measured on the first week after education and on the fifth week. Data from control group was collected during the period from Jan 11, 2 001 to Feb 15, 2001 and data from study group was collected during the period from Jan 12, 2001 to Feb 16, 2001. Measurement instrument for this study was developed by the author with the advice of specialist in order to measure self-efficacy and breast self-exam practice. Data analysis was done by using SPSS/10.0 PC program $with^2-$ test. t-test and ANCOVA. Proved results for hypothesis were as follows. 1) It was supported that "Study group educated by efficacy expectation promoting program has higher score in change rat e of self-efficacy than control group." (1 week after education F=18.395, p=.000 5 weeks after education F=28.972, p=.000) 2) It was supported that "Study group educated by efficacy expectation promoting program has higher score in change rate of exam practice than control group." (1 week after education F=37.984, p=.000 5 weeks after education F=28.972, p=.000) In conclusion, efficacy expectation promoting program for breast self-exam developed by this study may increase s elf-efficacy and breast self-exam practice.

• 치위생과학회지
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• 제22권3호
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• pp.191-198
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• 2022

Background: The purpose of this study was to evaluate the composition of the crystal phases of various calcium silicate-based materials (CSMs): ProRoot white MTA^Ⓡ (mineral trioxide aggregate) (WMTA), Ortho MTA^Ⓡ (OM), Endocem MTA^Ⓡ (EM), Retro MTA^Ⓡ (RM), Endocem Zr^Ⓡ (EN-Z), Biodentine^TM (BD), EZ-seal^TM (EZ), and OrthoMTA III (OM3). Methods: In a sample holder, 5 g of the powder sample was placed and the top surface of the material was packed flat using a sterilized glass slide. The prepared slides were mounted on an X-ray diffraction (XRD) instrument (D8 Advance; Bruker AXS GmbH, Germany). The X-ray beam 2θ angle range was set at 10~90° and scanned at 1.2° per minute. The Cu X-ray source set to operate at 40 kV and 40 mA in the continuous mode. The peaks in the diffraction pattern of each sample were analyzed using the software Diffrac (version 2.1). Then, the peaks were compared and matched with those of standard materials in the corresponding Powder Diffraction File (PDF-2, JCPDS International Center for Diffraction Data). A powder samples of the materials were analyzed using XRD and the peaks in diffraction pattern were compared to the Powder Diffraction File data. Results: Eight CSMs showed a similar diffraction pattern because their main component was calcium silicate. Eight CSMs showed similar diffraction peaks because calcium silicate was their main component. Two components were observed to have been added as radiopacifiers: bismuth oxide was detected in WMTA, OM, and EM while zirconium oxide was detected in RM, EN-Z, BD, EZ, and OM3. Unusual patterns were detected for the new material, OM3, which had strong peaks at low angles. Conclusion: It was caused by the presence of Brushite, which is believed to have resulted in crystal growth in a particular direction for a specific purpose.

• 한국수산과학회지
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• 제14권1호
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• pp.24-28
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• 1981

1980년 6월 20일부터 14일간, 어 청도산 미역을 모조로 유주자를 착생시킨 후, Keinetin, IAA및 2.4-D의 농도별배지에서 배양하여 배우체의 생장에 미치는 영향을 조사하였파. 그 결과를 간추리면 다음과 같다. 1. 발아율은 거의 $100\%$였으나 2.4-D 0.1 mg/l 또는 Keinetin 0.1, 1.0 또는 5.0 mg/1 배지에서는 발아관이 신장하지 않고 착생육자에서 바로 신생세포가 형성되었다. 2. 전체적 생장효과는 Keinetin 0.1 mg/l 배지에서 대조대비 $248.9\%$를 기록, 최고치를 표시하였고, IAA 1.0 mg/1($243.3\%$) 2.4-D에서는 0.05mg/l ($205.6\%$)가 가장 좋았다. 3. 웅성배우체에 미친 생장효과는 IAA 1.0mg/l가 $276.8\%$로서 최고치를 나타냈고 Keinetin 0.1mg/l($263.6\%$), 0.5mg/1($248.3\%$), 2.4-D 0.05mg/1($227.8\%$)의 순이 었다. 4. 자성배우체에서는 Keinetin이 효과적으로 작용하여 5.0mg/1($239.0\%$), 0.1mg/l ($222.0\%$), 0.05mg/l ($209.8\%$)를 기록 현저한 공률을 보여주고 있으나 IAA에서는 0.5mg/l($195.1\%$), 2.4-D에서는 0.05mg/1($164.6\%$)가 최고지를 기록하고 있어 대체로 웅성배우체에 비해 저조하고 배우체의 성에 따라 식물Hormone의 생장효과는 그 종류와 농도를 달리한다. 5. 생장조해농도는 2.4-D 0.5mg/l이상, IAA 0.5mg/1이상이고, Keinetin은 10.0mg/l 이상의 농도에서 생장이 크게 억제하고 있다. 6. 유효농도범의는 Keinetinol 0.01mg/1-5.0mg/l로 가장 넓고 공률곡선이 대단히 완만하며 IAA도 비교적 넓어 0.02mg/1와 1.0mg/l를 정점으로 생장효율 곡선이 S자형을 보여주고 있으나 2.4-D는 극히 좁아 0.05mg/1를 중심으로 급격히 저하하여 삼각곡선을 표시한다.

• Parasites, Hosts and Diseases
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• 제30권2호
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• pp.141-146
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• 1992

Kato후층도말변법 (M.C.T.S.)과 Stoll 회석충란계산법(D.E.C.T)으로 회충, 괸충, 간흡 충란 양성자 197명에 대하여 충란 정량검사를 실시하고 그 결과를 비교하여 상관함수식을 도출하였다. M.C.T.S.법에 소요된 검경시간은 표본 1매당 평균 12.6분으로 D.E.C.T.법의 14.6분보다 짧았으며, 층계부하가 낮은 감염자에 있어서도 위음성이 적었다. 각 윤충류 충란에 있어서 M.C.T.S.으로 얻은 결과를 대변 1 g당 충란수(E.P.G.)로 바꾸어 주는 전환함수식은 회충의 경우 E.P.G.=47.86×100.87 logM.C.T.S., 편충은 E.P.G.=41.69×100.83 logM.C.T.S., 간흡충은 E.P.G=63.10×100.55 logM.C.T.S.이었다. 장내 윤충류 감염에 대한 정량적 대변검사법으로 Kato후층도말변법이 Stoll희석충란계산 법보다 더 유용하다고 생각되었다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권2호
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• pp.132-136
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• 2004

The LAL (Limulus amebocyte lysate) test for the detection and quantification of endotoxin is based on the gelation reaction between endotoxin and LAL from a blood extract of Limulus polyphemus. The test is labor intensive, requiring dedicated personnel, a relatively long reaction time (approximately 1 h), relatively large volumes of samples and reagents and the detection of the end-point is rather subjective. To solve these problems, a miniaturized LOC (lab-on-a-chip) prototype, 62mm (L) ${\times}$ 18 mm (W), was fabricated using PDMS (polydimethylsiloxane) bonded to glass. Using this prototype, in which 2mm (W) ${\times}$ 44.3mm (L) ${\times}$ 100 $\mu\textrm{m}$ (D) microfluidic channel was constructed, turbidometric and chromogenic assay detection methods were compared, and the chromogenic method was found the most suitable for a small volume assay. In this assay, the kinetic-point method was more accurate than the end-point method. The PDMS chip thickness was found to be minimized to around 2 mm to allow sufficient light transmittance, which necessitated the use of a glass slide bonding for chip rigidity. Due to this miniaturization, the test time was reduced from 1 h to less than 10 min, and the sample volume could be reduced from 100 to ca. 4.4 ${\mu}$L. In summation, this study suggested that the LOC using the LAL test principle could be an alternative as a semi-automated and reliable method for the detection of endotoxin.

• 한국응용곤충학회지
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• 제25권1호
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• pp.41-46
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• 1986

고추 탄저병균(炭疽病菌)(Colletrichum denzatium f. sp. capsicum)의 발아(發芽)에 미치는 영양원(營養源) 및 환경요인(環境要因)의 영향(影響)을 구명(究明)코져 slide 발아법(發芽法)으로 실시(實施)하여 얻어진 결과(結果)는 다음과 같다. 1. 고추 탄저병균(炭疽病菌) 분생포자(分生胞子) 발아(發芽)의 온도범위(溫度範圍)는 $15{\sim}35^{\circ}C$ 이고 최적온도(最適溫度)는 $28^{\circ}C$, pH 범위(範圍)는 $4.5{\sim}8.0$이고 최적(最適) pH 는 5.5 였고 상대습도(相對濕度)는 포화(飽和)에 가까운 90% 이상(以上)의 상대습도(相對濕度)에서 발아율(發芽率)이 현저(顯著)히 좋았다. 2. 고추 탄저병균(炭疽病菌)의 포자발아(胞子發芽)는 PSB(potato sucrose broth), RPFD(red pepper fruit broth), GPFB(green pepper furit broth) 및 PLB (pepper leaf broth) 에서는 높은 발아율(發芽率)을 나타내었으나 살균증류수(殺菌蒸溜水)(D.W) 에서는 발아율(發芽率)이 현저(顯著)히 떨어지는 경향(傾向)이었다. 3. 탄소원(炭素源)과 질소원(窒素源)의 투여(投與)는 발아(發芽)에 절대적(絶對的)인 영향(影響)을 미치는 것으로 확인(確認)되었으며 K,P 및 S 등(等)의 무기염류(無機鹽類)는 뚜렷한 경향(傾向)을 보이지 않았다. 4. 탄소원(炭素源)이 발아(發芽)에 미치는 효과(效果)에서 단당류(單糖類)인 glucose 와 galactose 에서, 이당류(二糖類)인 lactose 에서 그리고 다당류(多糖類)인 가용성(可溶性) 전분(澱粉)에서 90% 이상(以上)의 높은 발아율(發芽率)을 나타내었다. 5. 현탁액(懸濁液)의 분생포자(分生胞子) 밀도(密度)가 $1{\times}10^4\;conidia/ml$ 일때 가장 높은 발아율(發芽率)을 나타내었고 $2{\times}10^4\;conidia/ml$ 이상(以上)일 때 발아율(發芽率)은 현저(顯著)히 감소(減少)되었으며 $5{\times}10^4\;conidia/ml$의 포자밀도(胞子密度)에서는 극(極)히 낮은 발아율(發芽率)을 보이는 것으로 미루어 포자밀도(胞子密度)의 증가(增加)가 self-inhibitor 로 작용(作用)하는 것으로 추측(推測)되며 기질(基質)을 제거(除去)한 분생포자(分生胞子)의 발아율(發芽率)은 기질(基質)을 제거(除去)하지 않은 분생포자(分生胞子)의 발아율(發芽率)보다 낮은 경향(傾向)을 보였으며 분생포자(分生胞子) 밀도(密度)가 증가(增加)할수록 더욱 현저(顯著)한 발아율(發芽率)의 감소(減少)를 가져왔다.

• 식물병연구
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• 제24권2호
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• pp.132-137
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• 2018

유자 검은점무늬병은 Diaporthe citri가 원인균이며, 과실에 발병하여 심각한 피해를 초래한다. 병자각으로부터 분생포자가 누출될 때, 습윤은 병원균 감염에 중요한 환경 요소이다. 발아과정 중 병포자가 건조에 처하거나 습윤중단이 발생할 때 발아율에 어떤 영향을 주는지 알아보았다. 건조 처리는 포자현탁액을 슬라이드 글라스에 0-48시간 동안 처리하였다. 습윤 중단 처리는 포자현탁액을 습윤에 10시간 먼저 노출한 후, 0-6시간 건조시켜 발아과정에서 습윤을 중단시켰다. 15시간 이상 건조 처리한 포자 발아율은 10% 이하였다. 3시간 이상 습윤 중단 처리된 포자 발아율은 35% 이하였다. 다른 식물병원성 곰팡이와 비교하면, D. citri는 건조에 민감한 곰팡이다. 유자 과피 배지 위에 다양한 온도(15, 20, 25, 30, $35^{\circ}C$)별로 습윤 존재시간(3, 6, 9, 12, 15, 18, 24 hr)을 다양하게 처리하여 포자발아율을 측정하였다. 발아율 10%와 50%를 달성하기 위한 최소 수분존재시간은 각각 4.5시간과 13시간이며, 이 때 적온은 각각 $29.2^{\circ}C$와 $29.1^{\circ}C$였다. 다양한 온도에서 발아율 10%와 발아율 50% 달성에 요구되는 최소 수분존재시간을 결정하는 추정식은 각각 $Wh_{10%}=0.082{\times}T^2-4.8025{\times}T+74.861$과 $Wh_{50%}=0.1093{\times}T^2-6.3762{\times}+106.08$이었다.