• Title/Summary/Keyword: SION

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Conjugation Process in Spirogyra varians Monitored with FITC-lectins(Zygnemataceae, Chlorophyta)

  • Yoou, Min-Chul;Kim, Man-Kyu;Kim, Gwang-Hoon
    • ALGAE
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    • v.24 no.1
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    • pp.39-45
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    • 2009
  • The conjugation processes of a filamentous freshwater green alga Spirogyra varians were examined using FITC-lectins. Conjugation comprised five steps: 1) aligning with adjacent filaments, 2) formation of conjugation protru-sion (papilla), 3) fusion of the protrusions, 4) formation of conjugation tube,and 5) formation of zygotes. Three lectins, ConA, RCA and UEA, showed considerable labeling during the progression of conjuation. FITC-ConA labeled the surfaces of filaments throughout the whole conjugation processes. FITC-RCA labeling was observed at the conjugation protrusions only after the papilla formation. Strong labeling continued until formationg of zygotes at the contacting area where the conjugation tube developed, but no labeling was detected on the surface of vegetative filaments. The labeling decreased gradually over time and disappeared when zygotes were formed. FITC-UEA showed similar labeling pattern with FITC-RCA except that weak labeling remained after zygote formation. Inhibition experiments using RCA, UEA which are complementary to sugars L-fucose and D-galactose, showed considerable decrease of conjugation (<32% vs. 70% in control). These results suggested that the lectin-carbohydrate recognition system might be involved in the conjugation of spirogyra varians.

A Study on the Relationship between Self-Esteem and Clothing Behavior -For Adult Males and Femalse- (자아존중과 의복행동간의 상관연구 -성인 남녀를 대상으로-)

  • Kim Soon-Ku;Park Jung-Soon
    • Journal of the Korean Society of Clothing and Textiles
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    • v.14 no.4 s.36
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    • pp.305-313
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    • 1990
  • The purpose of this study was to examine the relationship between self-esteem and demo- graphic variables of adult males and females upon clothing behaviors. The questionnaire were admistered to 249 unmarried females, 202 married females, 228 unmarried males, 179 married males in Pusan. The data was analyzed statisitically using to Pearson's correlation, t-test, Multiple regres-sion. The major findings of this research can be summarized as following; 1. There was significant relationship between the self-esteen and Conformity, Modesty, Management of the clothing behavior subscales. There was significant relationship among the clothing behavior subscales except Interest-Modesty, Conformity-Aesthetics, Conformity- Fashion, Modesty- Psychological dependence and Modesty-Aesthetics. 2. There was significant difference in the self-esteem and all clothing behavior subscales except Conformity according to sex. 3. There was significant difference in Interest, Psychological dependence, Fashion, Conform- ity and Modesty according to marriage status. 4. Sex and school career have significant effect on the self-esteem. Sex have significant effect on Importance, Interest, Modesty, psychological dependence, Aesthetics, Management and Fashion. School career significant effect on Conformity and income effect on Fashion. 5. Sex and school career have significant effect on the self-esteem as intervening variables, the clothing behaviors as dependent variables. Sex, age, school career and income have significant direct effect on the clothing behavior subscales.

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PECVD 공정에 의해 제작된 SION박막 특성 분석

  • Jeong, Jae-Uk;Chu, Seong-Jung;Park, Jeong-Ho
    • Proceedings of the Korean Vacuum Society Conference
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    • 2011.02a
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    • pp.123-124
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    • 2011
  • 플라즈마 화학적 기상 증착(plasma enhanced chemical vapor deposition)공정 중 NH3 gas flow rate, RF power, SiH4 gas flow rate을 고정시키고 N2O gas flow rate을 0 sccm부터 250 sccm까지 변화시키는 조건 하에 SiON박막을 증착한 후 그 투과율, 굴절률을 측정하고 분석하였다. N2O gas flow rate조건별 시편들은 증착율을 계산하여 350 nm 두께로 동일하게 SiON을 증착하였고, borofloat위에 SiON을 증착한 샘플은 투과율을, 실리콘기판 위에 SiON을 증착한 샘플로는 굴절률을 측정하였다. 투과율의 경우는 UV/Vis spectrometer를 이용해 633 nm, 1550 nm 두 가지 파장 대 모두에서 N2O gas flow rate이 가장 큰 250 sccm일 때 가장 높은 것을 알 수 있었고 N2O gas flow rate이 낮아질수록 투과율 또한 작아지는 경향을 보였다. 굴절률은 ellipsometer를 이용해 측정하였으며 633 nm 파장에서 N2O gas flow rate가 가장 낮은 0 sccm일 때 굴절률이 가장 큰 값을 가지고 N2O gas flow rate이 커질수록 굴절률은 지수함수적으로 감소되었다(n=1.837~1.494). 이는 N2O gas flow rate이 낮을수록 SiN계열에 커질수록 SiO2계열에 가까워지는 현상으로 이해된다. 이러한 실험분석 결과는 향후 실리카 도파로의 설계 및 최적화를 위해 사용될 수 있다.

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Cloning and Characterization of Actinorhodin Biosynthetic Gene Clusters from Streptomyces lividans TK24

  • Park, Kie-In
    • Animal cells and systems
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    • v.6 no.4
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    • pp.305-309
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    • 2002
  • Actinorhodin antibiotics produced by Streptomyces lividans TK24 are blue pigments with a weak antibiotic activity, derived from one acetyl-CoA and 15 malonyl-CoA units via a typical ployketide pathway. In an attempt to clone polyketide biosynthetic genes of S. lividans TK24, hybridizing fragments in the genomic DNA of S. lividans TK24 were detected by use of acn and act III polyketide synthase gene probes. Since typical aromatic polyketide bio-synthetic gene clusters are roughly 22-34 Kb long, we constructed in E. coli XL-Blue MR using the Streptomyces-E. coli bifunctional shuttle cosmid vector (pojn46). Then, about 5,000 individual E. coii colonies were thor-oughly screened with acrl-ORFI and actIII probes. From these cosmid libra-ries, 12 positive clones were identified. Restriction analysis and southern hybridization showed two polyketide biosynthetic gene clusters in this organism. These cosmid clones can be transformed into Streptomyces parvulus 12434 for expression test that identify product of actinorhodin biosynthetic genes by heterologous expression. Thus, heterologous expres-sion of a derivative compound of a actinorhodin biosynthetic intermediate was obtained in pKE2430. Expression of these compounds by the trans-formants was detected by photodiode array HPLC analysis of crude extracts.

The Influence of Family Cohesion and Adaptability on the Stress of Housewives Resulted from Home Management (도시주부의 가족응집력.적응력이 가정관리 스트레스에 미치는 영향)

  • 이정우
    • Journal of Families and Better Life
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    • v.17 no.3
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    • pp.63-82
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    • 1999
  • This study has a purpose to provide with the basis reference to decrease the housewives' stress and to improve the quality of family life by investigating the difference in the level of the stress resulted from home management according to background variables family cohesion and adaptability and by finding out relative importance of the variables which affect the stress of housewives resulted from home management. For all above the 550 self-reported questionnaires were distributed to housewives who have at least one child with their spouse in seoul. Finally 433 data were used for the final analysis. The major findings of this study are summarized as follows: First Family cohesion and adaptability show a significant difference by the housewives' education level the number of children communication satisfaction. Second housewives' stress is different significantly in accordance with the state of employment and communication satisfaction. Third it showed the lower the family coh sion and adaptability are the higher the housewives' stress is. And more influential variable of the two on home management stress is family cohesion. Fourth The background variables that influence in home management stress were education level perceived life level and age. Last As a result of looking into relative influence of the variables on the housewives' stress both psychological variable(perceived life level) and family cohesion were important variables in explaining housewives' home management stress.

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A Study on a neural-Net Based Call admission Control Using Fuzzy Pattern Estimator for ATM Networks (ATM망에서 퍼지 패턴 추정기를 이용한 신경망 호 수락제어에 관한 연구)

  • 이진이;이종찬;이종석
    • Proceedings of the Korean Institute of Intelligent Systems Conference
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    • 1998.10a
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    • pp.173-179
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    • 1998
  • This paper proposes a new call admission control scheme utilizing an inverse fuzzy vector quantizer(IFVQ) and neural net, which combines benefits of IFVQ and flexibilities of FCM(Fuzzy-C-Menas) arithmatics, to decide whether a requested call that is not trained in learning phase to be connected or not. The system generates the estimated traffic pattern of the cell stream of a new call, using feasible/infeasible patterns in codebook, fuzzy membership values that represent the degree to which each pattern of codebook matches input pattern, and FCM arithmatics. The input to the NN is the vector consisted of traffic parameters which is the means and variances of the number of cells arriving inthe interval. After training(using error back propagation algorithm), when the NN is used for decision making, the decision as to whether to accept or reject a new call depends on whether the output is greater or less then decision threshold(+0.5). This method is a new technique for call admi sion control using the membership values as traffic parameter which declared to CAC at the call set up stage, and is valid for a very general traffic model in which the calls of a stream can belong to an unlimited number of traffic classes. Through the simmulation. it is founded the performance of the suggested method outforms compared to the conventional NN method.

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Dynamic Threshold Value Decision in Image Binarization using Neural Network and Vi sion System (신경망과 비젼 시스템을 이용한 영상의 이진화에서 동적 임계값 설정)

  • 김영탁;문희근;김수정;김관형;탁한호;이상배
    • Proceedings of the Korean Institute of Intelligent Systems Conference
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    • 2002.12a
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    • pp.313-316
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    • 2002
  • 이동 물체의 이동 거리 추적이나 대상 물체의 인식과 판별 물체의 특징 추출과 같은 응용분야에서 컴퓨터(Computer)와 비젼시스템(vision system)을 이용한 영상 데이터 처리 분야에 대한 이용률이 증가하면서, 그에 따른 연구가 활발히 진행되고 있다. 따라서 CCD 카메라(Charge-Couple Device Camera)로부터 입력된 그레이 레벨(Gray Level)의 영상을 입력받아 처리과정을 거쳐 위치정보를 전송하는 과정에서 정확한 정보를 얻기 위한 전처리 과정 방법을 제안하고, 실제 시스템에 적용한 결과를 제시한다. 여기서 영상의 전처리 과정 중 입력 영상에서 불필요한 부분을 제거하거나, 배경과 대상물의 분리, 내포된 잡음을 없애기 위하여 흔히 이진화 방법을 많이 사용한다 특히 이진화 과정에서 그레이 레벨의 입력영상에서 히스토그램(histogram) 정보를 이용하여 영상의 이진화시의 임계값을 찾는 것은 아주 중요한 요인이다 따라서 본 논문에서는 신경회로망을 이용하여 실시간으로 CCD 카메라를 통하여 입력되는 그레이 레벨의 입력 영상에 대하여 동적으로 적당한 임계값을 .찾는 방법을 제안하고자한다. 또한 제안한 신경회로망을 이용한 임계값 추출 알고리즘(algorithms)을 구현한 시스템(system)에 적용하여 일반적인 방법과 비교 검토하고 응용 가능성을 확인한다.

Performance Analysis of Cyclostationary Interference Suppression for Multiuser Wired Communication Systems

  • Im, Gi-Hong;Won, Hui-Chul
    • Journal of Communications and Networks
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    • v.6 no.2
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    • pp.93-105
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    • 2004
  • This paper discusses cyclostationary interference suppression for multiuser wired communication systems. Crosstalk interference from digital signals in multipair cables has been shown to be cyclostationary. Many crosstalk equalization or suppression techniques have been proposed which make implicit use of the cyclostationarity of the crosstalk interferer. In this paper, the convergence and steady-state behaviors of a fractionally spaced equalizer (FSE) in the presence of multiple cyclostationary crosstalk interference are thoroughly analyzed by using the equalizer's eigenstructure. The eigenvalues with multiple cyclostationary interference depend upon the folded signal and interferer power spectra, the cross power spectrum between the signal and the interferer, and tile cross power spectrum between the interferers, which results in significantly different initial convergence and steady-state behaviors as compared to the stationary noise case. The performance of the equalizer varies depending on the relative clock phase of the symbol clocks used by the signal and multiple interferers. Measued characteristics as well as analytical model of NEXT/FEXT channel are used to compute the optimum and worst relative clock phases among the signal and multiple interferers.

The study of SiON thin film for optical properlies (SiON 박막의 광학적 특성에 대한 연구)

  • 김도형;임기주;김기현;김현석;김상식;성만영
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2001.11a
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    • pp.247-250
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    • 2001
  • We studied optical properties of SiON thin-film in the applications of optical waveguide. SiON thin-film was grown in 300$^{\circ}C$ by PECVD(plasma enhanced chemical vapor deposition) system. The change of SiON thin-film composition and refractive Index was studied as a function of varying NH$_3$ gas flow rate. As NH$_3$ gas flow rate was increased, Quantity of N and refractive index were increased at the same time. By the results, we could form the SiON thin-film to use of a waveguide with refractive index of 1.6. We analyzed the conditions of the thin-film with FTIR(fourier transform infrared) and OES(optical emission spectroscopy). N-H bonding(3390cm$\^$-1/) can be removed by thermal annealing. And we could observe the SiH bonding state and quantity by OES analysis in SiH$_4$

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Cardiac Safety Assessment of Medicinal Herbal Formulas Using hERG-HEK 293 cell (hERG 칼륨 채널 매개성 HEK 293 세포를 이용한 한약 처방의 심장 독성 평가 연구)

  • Choi, Susanna;Kwon, Oh-Bin;Lee, SION;Seo, Chang-Seob;Shin, Hyeun-Kyoo
    • The Journal of Korean Medicine
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    • v.40 no.2
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    • pp.94-105
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    • 2019
  • Objectives: The correlation between medicinal herbal formulas and a risk of cardiotoxicity has been controversial. Thus, this study investigated cardiac safety assessment of 52 most commonly used medicinal herbal formulas. Methods: We evaluated the in vitro effects of medicinal herbal formulas on recombinant human embryonic kidney 293 (HEK 293) cell line expressing human ether-a-go-go-related gene (hERG) potassium channel. Results and Conclusion: Our results demonstrated that all of 52 medicinal herbal formulas did not show inhibition of hERG current in hERG-HEK 293 recombinant cells. In conclusion, these safety data suggest that 52 medicinal herbal formulas were not associated with an increased risk of cardiotoxicity in hERG-HEK 293 recombinant cells.