• Korean Journal of Ecology and Environment
• /
• v.54 no.3
• /
• pp.170-189
• /
• 2021

Environmental DNA (eDNA) is a genetic material derived from organisms in various environments (water, soil, and air). eDNA has many advantages, such as high sensitivity, short investigation time, investigation safety, and accurate species identification. For this reason, it is used in various fields, such as biological monitoring and searching for harmful and endangered organisms. To collect eDNA from a freshwater ecosystem, it is necessary to consider the target organism and gene and a wide variety of items, such as on-site filtration and eDNA preservation methods. In particular, the method of collecting eDNA from the environment is directly related to the eDNA concentration, and when collecting eDNA using an appropriate collection method, accurate (good quality) analysis results can be obtained. In addition, in preserving and extracting eDNA collected from the freshwater ecosystem, when an accurate method is used, the concentration of eDNA distributed in the field can be accurately analyzed. Therefore, for researchers at the initial stage of eDNA research, the eDNA technology poses a difficult barrier to overcome. Thus, basic knowledge of eDNA surveys is necessary. In this study, we introduced sampling of eDNA and transport of sampled eDNA in aquatic ecosystems and extraction methods for eDNA in the laboratory. In addition, we introduced simpler and more efficient eDNA collection tools. On this basis, we hope that the eDNA technique could be more widely used to study aquatic ecosystems and help researchers who are starting to use the eDNA technique.

• Journal of The Korean Society of Integrative Medicine
• /
• v.9 no.3
• /
• pp.165-173
• /
• 2021

Purpose : Myofascial decompression is frequently mentioned as a method applied to cupping. The purpose of this study is to evaluate and compare active range of motion (AROM), muscle strength, and functional movement by applying myofascial decompression to the hamstrings. Methods : This study evaluated AROM, muscle strength, and functional movement by applying active movement myofascial decompression and static myofascial decompression to the dominant leg, respectively, in a crossover design conducted with normal adults (n=21) in their average 20s enrolled at G University in G city, Gyeongsangbuk-do. Active movement myofascial decompression was implemented for five minutes at a rate of 100 bpm to make the beats in flexion and extension respectively. Static myofascial decompression was only performed for five minutes while at rest. All of these interventions were performed at a cupping depth of two mm. After a one-week washout period, static was applied again to compare the same dependent variables. Results : Regarding AROM and muscle strength, both groups showed significant differences in the before and after results (p<.05). However, in the Functional Reach Aspect and Single Leg Hop test, the active movement myofascial decompression group showed better results. There was no statistical difference between the Active movement myofascial decompression group and Static myofascial decompression group in any dependent variable (p<.05). Conclusion : As a result of this experiment, both active movement myofascial decompression and static myofascial decompression had a positive effect on dependent variable. Therefore this study is meaningful in that it is easier and simpler to see the effect on flexibility, muscle strength, and functional movement just by implementing movement myofascial decompression.

• The Journal of Internal Korean Medicine
• /
• v.40 no.3
• /
• pp.369-389
• /
• 2019

Objectives: This study aimed to analyze the correlation between Ryodoraku diagnosis and three pattern identification questionnaires, namely, Spleen Qi Deficiency, Stomach Qi Deficiency, and Food Retention in functional dyspepsia (FD) patients. Methods: Forty FD patients who met the Rome IV diagnostic criteria for FD participated in this study. The Ryodoraku test was conducted, and three pattern identification questionnaires were filled up by all patients. The average Ryodoraku score was called total average (TA), and the scores on the Ryodoraku points were measured. The degree of Spleen Qi Deficiency, Stomach Qi Deficiency, and Food Retention was assessed by Spleen Qi Deficiency questionnaire (SQDQ), Scale for Stomach Qi Deficiency pattern (SSQD), and Food Retention Questionnaire (FRQ). Results: The TA scores in the SQDQ patient group were lower than those in the SQDQ control group (p=0.091). The TA scores in the SSQD patient group were higher than those in the SSQD control group (p=0.651). The TA scores in the FRQ patient group were lower than those in the FRQ control group (p=0.851). The scores on the LH5, RH5, and RH6 points were significantly lower in the SQDQ patient group than in the SQDQ control group. However, no significant difference was found in the Ryodoraku scores among the other groups. Conclusions: The results suggest that the low TA and low Ryodoraku scores on the LH5, RH5, and RH6 points could be a quantitative indicator to diagnose Spleen Qi Deficiency in FD patients in a simpler manner. Larger studies on the Ryodoraku test in FD patients, the health control group, and other pattern identification groups are required.

• Journal of the Korean Geotechnical Society
• /
• v.35 no.4
• /
• pp.15-26
• /
• 2019

The stability of the gravity quay wall against earthquakes is evaluated on the basis of the allowable displacement of the wall. To estimate the displacement caused by external forces, empirical equations based on the Newmark sliding block method or numerical analysis are widely used. In numerical analysis, it is possible to analyze precisely a complicated site and structure, but difficult to set the appropriate parameters and environments; there are limitations in obtaining reliable results, depending on one's level of expertise. The Newmark method, with only seismic motions, is widely used because it is simpler than numerical simulations when estimating permanent displacement. However, the empirical equations do not have any parameters for the response characteristics and sliding block of the structure, and sliding blocks being assumed as rigid bodies does not consider the nonlinear behavior of the soil and interaction with the structure. Therefore, in order to evaluate the seismic stability of the gravity quay wall, a newly-developed empirical equation is needed to overcome the above-mentioned limitations. In this study, numerical simulations are performed to analyze the response characteristics of the backfill of the structure, and to propose an optimal method of calculating the active area. For this purpose, finite element analyses were performed to analyze the response characteristics, and stress-strain relationships for various seismic motions. As a result, the response characteristics, sliding block, and failure surface of the backfill vary depending on the input seismic motions.

• Journal of Ocean Engineering and Technology
• /
• v.32 no.6
• /
• pp.447-457
• /
• 2018

In this study, a two-dimensional fully nonlinear transient wave numerical tank was developed using a desingularized indirect boundary integral equation method. The desingularized indirect boundary integral equation method is simpler and faster than the conventional boundary element method because special treatment is not required to compute the boundary integral. Numerical simulations were carried out in the time domain using the fourth order Runge-Kutta method. A mixed Eulerian-Lagrangian approach was adapted to reconstruct the free surface at each time step. A numerical damping zone was used to minimize the reflective wave in the downstream region. The interpolating method of a Gaussian radial basis function-type artificial neural network was used to calculate the gradient of the free surface elevation without element connectivity. The desingularized indirect boundary integral equation using an isolated point source and radial basis function has no need for information about the element connectivity and is a meshless method that is numerically more flexible. In order to validate the accuracy of the numerical wave tank based on the desingularized indirect boundary integral equation method and meshless technique, several numerical simulations were carried out. First, a comparison with numerical results according to the type of desingularized source was carried out and confirmed that continuous line sources can be replaced by simply isolated sources. In addition, a propagation simulation of a $2^{nd}$-order Stokes wave was carried out and compared with an analytical solution. Finally, simulations of propagating waves in shallow water and propagating waves over a submerged bar were also carried and compared with published data.

• BMB Reports
• /
• v.52 no.8
• /
• pp.496-501
• /
• 2019

Conventionally, immunotoxins have been produced as a single polypeptide from fused genes of an antibody fragment and a toxin. In this study, we adopted a unique approach of chemical conjugation of a toxin protein and an antibody fragment. The two genes were separately expressed in Escherichia coli and purified to high levels of purity. The two purified proteins were conjugated using a chemical linker. The advantage of this approach is its ability to overcome the problem of low recombinant immunotoxin production observed in some immunotoxins. Another advantage is that various combinations of immunotoxins can be prepared with fewer efforts, because the chemical conjugation of components is relatively simpler than the processes involved in cloning, expression, and purification of multiple immunotoxins. As a proof of concept, the scFv of trastuzumab and the PE24 fragment of Pseudomonas exotoxin A were separately produced using E. coli and then chemically crosslinked. The new immunotoxin was tested on four breast cancer cell lines variably expressing HER2. The chemically crosslinked immunotoxin exhibited cytotoxicity in proportion to the expression level of HER2. In conclusion, the present study revealed an alternative method of generating an immunotoxin that could effectively reduce the viability of HER2-expressing breast cancer cells. These results suggest the effectiveness of this method of immunotoxin crosslinking as a suitable alternative for producing immunotoxins.

• The Journal of Society for e-Business Studies
• /
• v.24 no.2
• /
• pp.71-89
• /
• 2019

In order to come up with satisfying product and improvement, firms use traditional marketing research methods to obtain consumers' opinions and further try to reflect them. Recently, gathering data from consumer communication platforms like internet and SNS has become popular methods. Meanwhile, with the development of information technology, mobile companies are launching new digital products for children to protect them from harmful content and provide them with necessary functions and information. Among these digital products, Kids Phone, which is a wearable device with safe functions that enable parents to learn childern's location. Kids phone is relatively cheaper and simpler than smartphone but it is noted that there are several problems such as some useless functions and frequent breakdowns. This study analyzes the reviews of Kids phones from domestic mobile companies, identifies the characteristics, strengths and weaknesses of the products, proposes improvement methods strategies for devices and services through SNS consumer analysis. In order to do that customer review data from online shopping malls was gathered and was further analyzed through text mining methods such as TF/IDF, Sentiment Analysis, and network analysis. Customer review data was gathered through crawling Online shopping Mall and Naver Blog/$Caf\acute{e}$. Data analysis and visualization was done using 'R', 'Textom', and 'Python'. Such analysis allowed us to figure out main issues and recent trends regarding kids phones and to suggest possible service improvement strategies based on sentiment analysis.

• Korean Journal of Agricultural and Forest Meteorology
• /
• v.22 no.4
• /
• pp.287-298
• /
• 2020

It is predicted that future climate warming will occur, and the subtropical climate zone currently confined to the south coast of Korea will gradually rise north. The shift of climate zone implies a change in area for cultivating crops. This study aimed to evaluate the current and future status of climate zones based on the high-resolution climate data of South Korea to prepare adaptation measures for cultivating crops under changing agricultural climate conditions. First, the climatic maps of South and North Korea were produced by using the high-resolution monthly maximum and minimum daily temperature and monthly cumulative precipitation produced during the past 30 years (1981-2010) covering South and North Korea. Then the climate zones of the Korean Peninsula were classified based on the Köppen climate classification. Second, the changes in climate zones were predicted by using the corrected monthly climate data of the Korean Peninsula (grid resolution 30-270m) based on the RCP8.5 scenario of the Korea Meteorological Administration. Köppen climate classification was applied based on the RCP8.5 scenario, the temperature and precipitation of the Korean Peninsula would continue to increase and the climate would become simpler. It was predicted that the temperate climate, appearing in the southern region of Korea, would be gradually expanded and the most of the Korean Peninsula, excluding some areas of Hamgkyeong and Pyeongan provinces in North Korea, would be classified as a temperate climate zone between 2071 and 2100. The subarctic climate would retreat to the north and the Korean Peninsula would become warmer and wetter in general.

• (The)Study of the Eastern Classic
• /
• no.35
• /
• pp.471-498
• /
• 2009

The aim of this study is to see how bamboo paintings developed and what is the iconographical meaning of them through bamboo Ti-hua-shi(poems on paintings) found in Sheng-Hua-Ji, a leading compilation of Ti-hua-shi(poems on paintings) of Song Dynasty. Bamboo paintings give shapes in a simpler and more direct way in drawing, which means bamboo paintings reveal concretely more characteristics of Ti-hua-shi (poems on paintings). Bamboo paintings, which flourished during Northern Song Dynasty, often had the image of the dragon, specially a lying dragon or a winding dragon, which suggests a great man in obscurity. Snow-laden bamboos were also a symbol of a great man, very wise man in Confucian tradition. This shows Confucian ideal examples were embodied through bamboo paintings. Another aspect of bamboo paintings is that bamboo paintings were regarded as a means of self-expression, which identified Confucian sadaebu (scholar-officials) who advocated simplicity and austerity in their life. Contrary to professional painters, who added color and decoration to their paintings, Confucian scholars of Song Dynasty reduced color and embellishment in their bamboo paintings in black-and-white to reveal their own identity, focusing on spirituality rather than the image itself. Therefore, drawing and appreciating bamboo paintings were highly respectable pastime among the literati of Song Dynasty. In short, Bamboo paintings in Song Dynasty were not only a Confucian symbol of a wise and virtuous man but also reflected the taste of Song sadaebu class who thought of bamboo as a symbol for moral and cultural responsibility of Confucian society.

• Journal of the Korea Institute of Information Security & Cryptology
• /
• v.21 no.1
• /
• pp.135-141
• /
• 2011

Recent advances in mobile devices and development of various mobile applications dealing with private information of users made user authentication in mobile devices a very important issue. This paper presents a new user authentication method based on touch screen interfaces. This method uses for authentication the PIN digits as well as the exact locations the user touches to input these digits. Our method is fully compatible with the regular PIN entry method which uses numeric keypads, and it provides better usability than the behavioral biometric schemes because its PIN registration process is much simpler. According to our experiments, our method guarantees EERs of 12.8%, 8.3%, and 9.3% for 4-digit PINs, 6-digit PINs, and 11-digit cell phone numbers, respectively, under the extremely conservative assumption that all users have the same PIN digits and cell phone numbers. Thus we can guarantee much higher performance in identification functionality by applying this result to a more practical situation where every user uses distinct PIN and sell phone number. Finally, our method is far more secure than the regular PIN entry method, which is verified by our experiments where attackers are required to recover a PIN after observing the PIN entry processes of the regular PIN and our method under the same level of security parameters.