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The Improvement of Sexing PCR Conditions and Survival Rate of Blastomere Separation Method in the Bovine Embryo (소 수정란의 할구 분리방법에 따른 생존율 및 성판별 PCR의 개선)

  • Kim, Sang-Hwan;Kim, Kyong-Lae;Lee, Ho-Jun;Jung, Kyoung-Sub;Baek, Jun-Seok;Jung, Duk-Won;Kim, Dae-Eun;Lee, Deuk-Hwan;Yoon, Jong-Taek
    • Journal of Embryo Transfer
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    • v.28 no.3
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    • pp.199-205
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    • 2013
  • The present study was conducted to compare on embryo survival rates by blastomere isolation methods, and establish the optimal PCR procedure for perform the sexing of bovine blastocysts produced by IVF. IVF embryos used in the study was used the Bisected or Sliced methods for blastomere isolation, and the survival rates of blastocyst with rapid way of sexing PCR was assessed. In the present study for survival rates in blastocyst was the total cleavage rate was 75% and a blastocyst development among cleaved embryos was 40%. Survival rate of embryos treated with intact, bisected or sliced method was 100, 63.3 or 81.3%, respectively. Therefore, survival rate of embryos treated with sliced method was higher compared to that of embryos treated with bisected method. The sexing rate of female or male was not significantly different between S4BFBR primer and BSY + BSP primer (1.75 : 1 vs. 1.43 : 1), respectively. Because of the PCR amplification using the S4BFBR primer was simpler method than multiplex PCR amplification method. Furthermore, the accuracy of sexing rate and reduction of PCR work time between 2-step and 3-step of PCR methods was 98.0% / 1.5 hr and 97.0% / 3.5 hr, respectively. Based on these results, it can be suggested that the sliced and PCR methods we developed was very effective method to reduce time consuming and procedure of PCR amplification for sexing with the increase of survival rate on the blastocyst.

Development of Semi-cooked Pork using Steam Oven for Food Service System (오븐을 이용한 단체급식용 반조리식 제육구이 개발)

  • Kim, Jeong-Mee;Kim, Ok-Hee
    • Food Science of Animal Resources
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    • v.29 no.1
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    • pp.62-67
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    • 2009
  • This study was undertaken to develope a semi-cooked pork using oven to provide a more convenient recipe for food service systems. Pork sliced 2 or 4 mm thick prepared with a Korean traditional kochujang sauce was broiled in an oven, steam oven or on a pan. 4 mm-thick meat that was broiled in oven for 4 min at $170^{\circ}C$, obtained higher scores in sensory and color evaluations. In labor-concentrated food service kitchens, the inner temperature of the pork at $85^{\circ}C$ provoked the highest satisfaction. The assessments of color L was ordered as steam oven, oven and pan broiling. And a- and b-values of oven broiling were higher than those of the steam oven or pan broiling. The color values of the steam oven-broiled pork sliced 4 mm-thick were significantly higher than those of the pan-broiled (p<0.05). In sensory evaluation, the taste score of steam oven was better than other broiling with significant differences. Furthermore pork broiling in oven was tender with low score in cohesiveness and chewiness. Overall acceptability was ordered as pan, steam oven, and oven broiling. Thus it was expectable that international Korean traditional food can be made using oven for food service system. For the diversity of the menu in food service, however it need to develope the more convenient and simpler recipes with semi-cooked Korean food.

The Influence of Sediment Control Dam Dredging on Benthic Macroinvertebrate Communities of Mountain Stream (사방댐 준설이 저서성 대형무척추동물 군집에 미치는 영향)

  • Kim, Bong Sung;Lee, Chang Woo;Seo, Eul Won;Lee, Jong Eun
    • Journal of Korean Society of Forest Science
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    • v.101 no.3
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    • pp.454-460
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    • 2012
  • This study was conducted for determining the influence of Sediment Control Dam dredging on benthic macroinvertebrate communities in mountain stream of Gyeongsangbuk-do. The sediment control dams were surveyed before and after dredging from mountain streams of Yecheon, Yeongju and Bonghwa in Northern part of Gyeongsangbuk-do. Sampling was taken at upper and down from 3 experimental sites and 2 control sites during May to August 2011. The identified species before dredging were 56 belonged to 31 families, 11 orders, 6 classes and 4 phyla, but after dredging it has decreased to 51 species belonged to 27 families, 10 orders, 5 classes and 4 phyla. The microhabitat damage of benthic macroinvertebrates occurred at experimental sites after dredging of sediment control dams led to reduction of the number of species and individuals. Especially, upper stream of experimental sites showed the reduction of an average of 38% species. In general, species diversity indices and species richness indices decreased after dredging; however, dominance indices increased at experimental sites. In the upper stream of experimental sites, the microhabitat damaged and became pool due to dredging. Also, velocity of flow decreased and the river bed became simply due to the sedimentary matter being finer than sand and silt. As a result, composition of functional feeding groups and functional feeding groups were relatively simpler at upper stream and the degree of community differences was greater between upper and down stream. Also, Chironomidae spp., Ephemeridae spp., and Gomphidae spp. to prefer where slow velocity of flow and lentic increased in a greater rate, and the EPT/C index to indicate the balance of the community decreased.

An Educational Platform for Digital Media Prototype Development: an analysis and a usability study (디지털 미디어 콘텐츠 개발을 위한 교육용 플랫폼의 활용성)

  • Kim, Na-Young
    • The Journal of the Korea Contents Association
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    • v.11 no.8
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    • pp.77-87
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    • 2011
  • The advent of new platforms each year along with the advancement of technology provides a new opportunity for digital media designers to develop creative and innovative contents. This phenomenon affect the same way the students that major in the digital media, and the use of the platforms that is based on the new technology in the development of contents gives a newer and useful opportunity for learning to the students who recently study the digital media area. As the main technology of the recent digital media that attract many students' attention, we are presenting virtual reality display, movement cognition, physical engine and the gesture interface, and developed the consolidated platform based on these four technologies, and designed them in a way that can be more easily implemented in a simpler way. In order to study the efficiency of the platform with the objective of the development of digital media contents, we have developed four different prototype contents, and have measured based on the user's preference, efficiency and satisfaction. In the results of usability evaluation, functionality, effectiveness, efficiency, satisfaction were rated as 'high'. This results shows that the suggested 3D platform environment provides students to develop a rapid prototype fast and easy, and this may have a positive influence on students major in the digital media to conduct creative development research.

An analysis of trends in wetland function assessments and further suggestions (습지 기능 평가의 동향 분석 및 제언)

  • Hong, Mun Gi;Kim, Jae Geun
    • Journal of Wetlands Research
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    • v.19 no.1
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    • pp.1-15
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    • 2017
  • Wetland function assessment is not only a basic step to understand wetland ecosystems in detail but also an important process as a base of the term, ecosystem service to recognize wetland ecosystems as valuable and useful resources and goods for human being. WET (wetland evaluation technique), EMAP (environmental monitoring assessment program)-wetlands, and HGM (hydrogeomorphic method) were developed as pioneer wetland function assessments in U.S. at the end of $20^{th}$-century. RAMs (rapid assessment methods) became a major function assessment tool which is relatively simpler and easier assessment tool at the beginning of $21^{th}$-century. After that, the hierarchy of three levels of assessment (landscape assessment, rapid assessment, and intensive assesment) has been prepared and strategically utilized according to the objectives and purposes of function assessments. In South Korea, RAM and HGM were used to assess wetland functions with reforming a couple of items and contents at 2001. And, modified and reformed function assessments have been developed to complement and improve upon the existing RAMs and HGMs. Via the trend analysis on wetland function assessments, some needs which require supplements in terms of function assessment are pointed out: 1) wetland function assessments using useful indicators such as birds are needed with considering our environmental characteristics. 2) optimized wetland function assessments for coastal wetlands are also needed. 3) the network construction and further expansion to lead communications and co-operations between researchers and policy makers is needed in the field of wetland function assessment.

An Effective Microcalcification Detection in Digitized Mammograms Using Morphological Analysis and Multi-stage Neural Network (디지털 마모그램에서 형태적 분석과 다단 신경 회로망을 이용한 효율적인 미소석회질 검출)

  • Shin, Jin-Wook;Yoon, Sook;Park, Dong-Sun
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.29 no.3C
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    • pp.374-386
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    • 2004
  • The mammogram provides the way to observe detailed internal organization of breasts to radiologists for the early detection. This paper is mainly focused on efficiently detecting the Microcalcification's Region Of Interest(ROI)s. Breast cancers can be caused from either microcalcifications or masses. Microcalcifications are appeared in a digital mammogram as tiny dots that have a little higher gray levels than their surrounding pixels. We can roughly determine the area which possibly contain microcalifications. In general, it is very challenging to find all the microcalcifications in a digital mammogram, because they are similar to some tissue parts of a breast. To efficiently detect microcalcifications ROI, we used four sequential processes; preprocessing for breast area detection, modified multilevel thresholding, ROI selection using simple thresholding filters and final ROI selection with two stages of neural networks. The filtering process with boundary conditions removes easily-distinguishable tissues while keeping all microcalcifications so that it cleans the thresholded mammogram images and speeds up the later processing by the average of 86%. The first neural network shows the average of 96.66% recognition rate. The second neural network performs better by showing the average recognition rate 98.26%. By removing all tissues while keeping microcalcifications as much as possible, the next parts of a CAD system for detecting breast cancers can become much simpler.

Image Warping Using Vector Field Based Deformation and Its Application to Texture Mapping (벡터장 기반 변형기술을 이용한 이미지 와핑 방법 : 텍스쳐 매핑에의 응용을 중심으로)

  • Seo, Hye-Won;Cordier, Frederic
    • Journal of KIISE:Computer Systems and Theory
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    • v.36 no.5
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    • pp.404-411
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    • 2009
  • We introduce in this paper a new method for smooth foldover-free warping of images, based on the vector field deformation technique proposed by Von Funck et al. It allows users to specify the constraints in two different ways: positional constraints to constrain the position of a point in the image and gradient constraints to constrain the orientation and scaling of some parts of the image. From the user-specified constraints, it computes in the image domain a C1-continuous velocity vector field, along which each pixel progressively moves from its original position to the target. The target positions of the pixels are obtained by solving a set of partial derivative equations with the 4th order Runge-Kutta method. We show how our method can be useful for texture mapping with hard constraints. We start with an unconstrained planar embedding of a target mesh using a previously known method (Least Squares Conformal Map). Then, in order to obtain a texture map that satisfies the given constraints, we use the proposed warping method to align the features of the texture image with those on the unconstrained embedding. Compared to previous work, our method generates a smoother texture mapping, offers higher level of control for defining the constraints, and is simpler to implement.

A Study of the Automatic Extraction of Hypernyms arid Hyponyms from the Corpus (코퍼스를 이용한 상하위어 추출 연구)

  • Pang, Chan-Seong;Lee, Hae-Yun
    • Korean Journal of Cognitive Science
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    • v.19 no.2
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    • pp.143-161
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    • 2008
  • The goal of this paper is to extract the hyponymy relation between words in the corpus. Adopting the basic algorithm of Hearst (1992), I propose a method of pattern-based extraction of semantic relations from the corpus. To this end, I set up a list of hypernym-hyponym pairs from Sejong Electronic Dictionary. This list is supplemented with the superordinate-subordinate terms of CoroNet. Then, I extracted all the sentences from the corpus that include hypemym-hyponym pairs of the list. From these extracted sentences, I collected all the sentences that contain meaningful constructions that occur systematically in the corpus. As a result, we could obtain 21 generalized patterns. Using the PERL program, we collected sentences of each of the 21 patterns. 57% of the sentences are turned out to have hyponymy relation. The proposed method in this paper is simpler and more advanced than that in Cederberg and Widdows (2003), in that using a word net or an electronic dictionary is generally considered to be efficient for information retrieval. The patterns extracted by this method are helpful when we look fer appropriate documents during information retrieval, and they are used to expand the concept networks like ontologies or thesauruses. However, the word order of Korean is relatively free and it is difficult to capture various expressions of a fired pattern. In the future, we should investigate more semantic relations than hyponymy, so that we can extract various patterns from the corpus.

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Lightweight Loop Invariant Code Motion for Java Just-In-Time Compiler on Itanium (Itanium상의 자바 적시 컴파일러를 위한 가벼운 루프 불변 코드 이동)

  • Yu Jun-Min;Choi Hyung-Kyu;Moon Soo-Mook
    • Journal of KIISE:Software and Applications
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    • v.32 no.3
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    • pp.215-226
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    • 2005
  • Loop invariant code motion (LICM) optimization includes relatively heavy code analyses, thus being not readily applicable to Java Just-In-Time (JIT) compilation where the JIT compilation time is part of the whole running time. 'Classical' LICM optimization first analyzes the code and constructs both the def-use chains and the use-def chains. which are then used for performing code motions. This paper proposes a light-weight LICM algorithm, which requires only the def-use chains of loop invariant code (without use-def chains) by exploiting the fact that the Java virtual machine is based on a stack machine, hence generating code with simpler patterns. We also propose two techniques that allow more code motions than classical LICM techniques. First, unlike previous JIT techniques that uses LICM only in single-path loops for simplicity, we apply LICM to multi-path loops (natural loops) safely for partially redundant code. Secondly, we move loop-invariant, partially-redundant null pointer check code via predication support in Itanium. The proposed techniques were implemented in a JIT compiler for Itanium processor on ORP (Open Runtime Platform) Java virtual machine of Intel. On SPECjvrn98 benchmarks, the proposed technique increases the JIT compilation overhead by the geometric mean of 1.3%, yet it improves the total running time by the geometric mean of 2.2%.

A Study on Water Level Control of PWR Steam Generator at Low Power Operation and Transient States (저출력 및 과도상태시 원전 증기발생기 수위제어에 관한 연구)

  • Na, Nan-Ju;Kwon, Kee-Choon;Bien, Zeungnam
    • Journal of the Korean Institute of Intelligent Systems
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    • v.3 no.2
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    • pp.18-35
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    • 1993
  • The water level control system of the steam generator in a pressurized water reactor and its control problems are analysed. In this work the stable control strategy during the low power operation and transient states is studied. To solve the problem, a fuzzy logic control method is applied as a basic algorithm of the controller. The control algorithm is based on the operator's knowledges and the experiences of manual operation for water level control at the compact nuclear simulator set up in Korea Atomic Energy Research Institute. From a viewpoint of the system realization, the control variables and rules are established considering simpler tuning and the input-output relation. The control strategy includes the dynamic tuning method and employs a substitutional information using the bypass valve opening instead of incorrectly measured signal at the low flow rate as the fuzzy variable of the flow rate during the pressure control mode of the steam generator. It also involves the switching algorithm between the control valves to suppress the perturbation of water level. The simulation results show that both of the fine control action at the small level error and the quick response at the large level error can be obtained and that the performance of the controller is improved.

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