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Isolation of a Quinone-rich Fraction from Ardisia crispa Roots and its Attenuating Effects on Murine Skin Tumorigenesis

  • Yeong, Looi Ting;Hamid, Roslida Abdul;Yazan, Latifah Saiful;Khaza'ai, Huzwah
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.4
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    • pp.2301-2305
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    • 2013
  • Ardisia crispa (Family: Myrsinaceae) is an evergreen, fruiting shrub that has been traditionally used as folklore medicine. Despite a scarcity of research publications, we have succeeded in showing suppressive effects on murine skin papillomagenesis. In extension, the present research was aimed at determining the effect of a quinone-rich fraction (QRF) isolated from the same root hexane extract on both initiation and promotion stages of carcinogenesis, at the selected dose of 30 mg/kg. Mice (groups I-IV) were initiated with a single dose of 7,12-dimethylbenz(${\alpha}$)anthracene (DMBA, $100{\mu}g/100{\mu}l$) followed by repeated promotion of croton oil (1%) twice weekly for 20 weeks. In addition, group I (anti-initiation) received QRF 7 days before and after DMBA; group II (anti-promotion) received QRF 30 minutes before each croton oil application; group III (anti-initiation/promotion) was treated with QRF as a combination of group I and II. A further two groups served as vehicle control (group V) and treated control (group VI). As carcinogen control, group IV showed the highest tumor volume ($8.79{\pm}5.44$) and tumor burden ($3.60{\pm}1.17$). Comparatively, group III revealed only 20% of tumor incidence, tumor burden ($3.00{\pm}1.00$) and tumor volume ($2.40{\pm}1.12$), which were significantly different from group IV. Group II also showed significant reduction of tumor volume (3.11), tumor burden (3.00) and tumor incidence (11.11%), along with prominent increase of latency period of tumor formation (week 12). Group I, nonetheless, demonstrated marked increment of tumor incidence by 40% with prompted latency period of tumor formation (week 7). No tumor formation was observed in groups V and VI. This study provided clear evidence of inhibitory effects of QRF during promotion period which was in agreement with our previous findings. The mechanism(s) underlying such effects have yet to be elucidated.

Seeding of the Woody Plants for the Quick-coverage of the Slopes (비탈면 조기수림화를 위한 녹화용 식물의 활용에 관한 연구)

  • Kim, Namchoon;Yoon, Jungseo;Bae, Sunwoo;Son, Won-Ju;Jung, Sungchul
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.5 no.6
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    • pp.72-85
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    • 2002
  • This study was conducted to investigate the germination rates and ground coverage rates of the 16 native wild flower, herbaceous, shrub and woody plants according to temperature and seeding timing for the revegetation and rehabilitation of the roadside slopes. Also, this study was conducted to suggest design criteria to select revegetation plants and to decide proper seed mixture for ecological restoration of the disturbed manmade slopes as a environmentally friendly construction. The results are summarized as follows. 1. Most of the plants germinated after 10~14 days. Woody plants germinated more slowly than wild flowers and herbaceous plants because of the hard seed. 2. Most of the plants showed the highest percentage of the germination rates under $15/25^{\circ}C$ temperature. The next was in order of under the $20/30^{\circ}C$ temperature, normal temperature(October) and $25/35^{\circ}C$ temperature. 3. At the chamber experiment, the Sorbaria sorbifolia var. stellipila showed the highest germination rates. Most of the native wild flowers germinated well and showed high germination rates under the various temperature. 4. As the field germination experiments, the seeding at August shows higher germination rates than that of the seeding at September, but showed lower surviving percentage of the germinated seedlings and lower ground coverage percentage than those of the seeding at September. After one year, Chionanthus retusus, Acer palmatum, Albizzia julibrissin are germinated and showed 10% coverage rates. So, they can be used as revegetation plants for the restoration works. 5. As seed mixture experiment, it was not effective only to increase the proportion of the ratio of the tree seeds, relatively high price, for making woody vegetation. It would be more successful to make vegetation structure by natural competition among wild flowers, shrubs and trees. 6. The excessive dominance of trees in revegetation works may destroy the lower layer of vegetation and it will be undesirable on the species diversity. It is more important to recover the species diversity of the plant community by seed mixture with the considering the germination, the growth characteristics and the correlation effect among revegetation plants. 7. To recover the manmade slopes quickly, increasing the ratio of the wild flower was recommended.

Habitat and Distribution Feature of Endangered Species Leontice microrhyncha S. Moore (멸종위기종 한계령풀(Leontice microrhyncha S. Moore)의 서식지 및 분포 특성)

  • Lee, Sang-Hoon;Yeon, Myung-Hun;Shim, Jae-Kuk
    • Korean Journal of Environment and Ecology
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    • v.25 no.6
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    • pp.819-827
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    • 2011
  • Climate change is recognised as the best serious environmental problem in recent time, and high alpine or high latitudinal organisms especially endangered by its change. Leontice microrhyncha is recorded one of the endangered species by the Ministry of Environment Korea. We surveyed ten L. microrhyncha populations distributed at Taebaek Mountains, high mountain area in Kangwon province. L. microrhyncha is distributed 940m~1350m high altitude which equivalent of Warmth Index $53^{\circ}C{\cdot}month$ to $75^{\circ}C{\cdot}month$, the range of conspicuous cool temperate forest zone. The plant species distributed at slope of $5^{\circ}{\sim}23^{\circ}$ on northeast slopes. The vegetation structure at tree layer of L. microrhyncha distribution area is dominated by Quercus mongolica, Betula costata, Cornus controversa, Acer mono in tree layer, and by Staphylea bumalda, Deutzia glabrata, Stephanandra incisa in shrub layer. The species diversity of herb layer of each L. microrhyncha population showed from 0.21 to 0.98, and the importance value of L. microrhyncha in each population was the highest at blooming time of L. microrhyncha. These results will provide the basic information for the development of conservation strategies for this endangered species.

Vegetation Structure of the Bulguksa Buddhist Temple Forest in the Gyeongju National Park (경주국립공원 불국사 사찰림의 식생구조)

  • Kang, Hyun-Mi;Choi, Song-Hyun;Lee, Soo-Dong;Cho, Hyun-Seo;Kim, Ji-Suk
    • Korean Journal of Environment and Ecology
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    • v.26 no.5
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    • pp.787-800
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    • 2012
  • The purpose of this study was to investigate the vegetation structure of Bulguksa around Buddhist Temple Forest in the Gyeongju National Park. To do so, forty-two plots($100m^2$) were set up and surveyed. The surveyed plots were divided into four groups according to the analysis of classification by TWINSPAN; (I) Pinus densiflora-Pinus koraiensis community, (II) Pinus densiflora community, (III) Pinus densiflora-Acer palmatum community, (IV) Acer palmatum-Pinus densiflora community. The results of vegetation structure analysis were; Bulguksa around Buddhist Temple Forest in the Gyeongju National Park were dominated by Pinus densiflora. IV community, influx of Acer palmatum in Pinus densiflora community, Acer palmatum-Pinus densiflora community are believed to be a change to the community. But, recent spontaneously is growing Quercus variabilis, Quercus aliena, Quercus serrata, Quercus mongolica in understory and shrub layer. Later, it is expected that Pinus densiflora competition. The forest vegetation age of the study area is Pinus densiflora were dominant trees in forest was 30~100 years, old while that of Acer palmatum was 30~36 years old.

Estimate Feed Value of Herbage from Shrubby Area (관목지 목초본류의 사료가치에 관한 평가)

  • Kim, Yong Kook;Jeon, Chang Gie
    • Korean Journal of Agricultural Science
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    • v.8 no.2
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    • pp.164-170
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    • 1981
  • In order to develop feed source from shrubby area of mountain for dairy cow, some shrub and grass were harvestied by stage of maturity and analyzed the chemical composition of herbage, and fed to milking cow to know the effect on the body weight and milk production. And the results were summarized as follow. 1. The dry matter content of green herbage was ranged average 25~50% and Smilax china and Cocculus thunbergii were the lowerest as 26.25% and 26.36% respectively and Quercus serrata was the highest as 47.19% of the species. 2. The average protein content of herbage was widely ranged from about 5% to 20%. And Pueraria thunbergiana and Albizzia julibrissin were higher as nearly 20% and Miscanthus sinesis and Arundinella hirta were lower as nearly 5~7%. 3. The average crude fiber content of most species was rarged 20~30%. And Corylus heterophylla and Smilax china were lower as about 20% and Miscanthus sinensis and Arundinella hirta were higher as nearly 37%. 4. The crude protein content of most species was decreased and the fiber content was increased by abvanced the stage of maturity. And also moisture content of green herbage was decreased by seasonal changed. 5. When air dry herbage of 5 kg per head/day was substituted for rice straw, the weight of cows and milk yield and solid not fat content were Slightly increased.

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Ecological Characteristics and Change for fifteen Years$(1990$\sim2004)$ of Plant Community Structure of the Pinus densiflora S. et Z. Forest in Namsan, Seoul (서울시 남산소나무림 생태적 특성 및 15년간(1990$\sim$2004년) 식생구조 변화분석)

  • Lee Kyong-Jae;Kim Jeong-Ho;Han Bong-Ho
    • Korean Journal of Environment and Ecology
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    • v.19 no.3
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    • pp.312-326
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    • 2005
  • The purpose of this study is to provide data for conservation and ecological management of Pinus densiflora forest by analyzing ecological characteristics and the change of Pin us densiflora community structure for fifteen years in Namsan(Mt.). The actual vegetation of Pinus densiflora forest was divided into total six types and fifteen detail types. According to the results of TWINSPAN analysis of Pinus densiflora forest, there were fourty-four plots(unit: 400 m') and it was classified into five community types. The dominant species were Pinus densiflora in canopy layer, Styrax japonica in the understory layer and Stephanandra incisa with Rhododendron mucronujatum in the shrub layer. The index of shannon's diversity was from 1.5980 to 1.1485 per $400m^2$and the range of species number was from seventeen to twenty-two. As a result of the change of Pin us densinora forest structure, the importance percentage(1.p.) of Pinus densi. nora$(I.P.:\;77.9\%\rightarrow50.6\%) was decreased, Styraxjaponica$(I.P.:\;5.3\%\rightarrow22.6\%), Prunus sargen $(I.P.:\;1.5\%\rightarrow9.2\%) and Stephanandra incisa$(I.P.:\;3.5\%\rightarrow7.7\%) were increased for fifteen years. Based on standard of $2,000m^2$unit area, shannon's diversity changed 1.1719 into 0.8829 and species number changed thirty-four into twenty-one.

Recently Augmented Natural Habitat of Abeliophyllum distichum Nakai in Yeoju-si, Gyunggi-do, Korea (미선나무(Abeliophyllum distichum Nakai)의 새로운 자생지 보고 - 경기도 여주시 자생지-)

  • Lee, Ho-Young;Kim, Tae-Gwan;Oh, Chung-Hyeon
    • Korean Journal of Environment and Ecology
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    • v.28 no.1
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    • pp.62-70
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    • 2014
  • This study was carried out to analysis the vegetation status, the habitat size and the meaning of new natural habitat, and to provide basic data for conservation or management on the newly augmented natural habitat of Abeliophyllum distichum Nakai in Yeoju-si, Gyunggi-do, Korea. A. distichum is a Korean monotypic endemic species. Most natural habitats of A. distichum have been reported in South-Central area around Chungcheong-do. The extent of occurrence of A. distichum can be enlarged to Central area due to the Yeoju-si habitat. The new habitat is located on a hillock which is composed of rock field at $37^{\circ}20{\sim}21^{\prime}N$, $137^{\circ}43{\sim}21^{\prime}E$, and 99~120m a.s.l.. The habitat size is small as about $530m^2$, and a small stream runs aside. The number of individuals of A. distichum was about 1,200. But most individuals were smaller than 0.5m height, so just about 300 individuals are taller than 1.0m height. A. distichum on Yeoju-si seemed to be maintained and distributed by vegetative propagation from elongated roots of branches. Pinus rigida and Quercus aliena dominated tree layer, and Lindera obtusiloba, Q. aliena and Acer tatricum appeared frequently in subtree layer. The shrub layer was dominated by A. distichum with Stephanandra incisa, Euonymus alatus for. ciliatodentatus, Ligustrum obtusifolium, etc. The coverage of herbaceous layer was low.

The Structure of Plant Community of the Woonsooam Valley in Jogyesan (Mt.), Suncheon (순천시 조계산 운수암 계곡부 식물군집구조)

  • Han, Bong-Ho;Choi, Jin-Woo;Noh, Tai-Hwan;Hur, Ji-Yeon
    • Korean Journal of Environment and Ecology
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    • v.28 no.1
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    • pp.45-54
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    • 2014
  • This study was carried out to investigate the ecological succession sere and conservative value, and to provide the basic data in Woonsooam Valley, in Jogyesan (Mt.) (altitude 884m), Suncheon City, Korea by analysing the structure of the plant community. Fifteen plots (size is $20m{\times}20m$) were set up at an altitude of range from 255m to 495m. As a result of analysis of DCA which is one of the ordination technique, the plant communities were divided into five groups which are community I (Pinus densiflora community), community II (Quercus variabilis-Quercus serrata community), community III (Q. serrata-Q. variabilis community), and community IV (Carpinus tschonoskii-Q. serrata community) and community v(Deciduous broad-leaved forest community). We found out that the vegetation of the study site located in the South Temperate Climate Zone. The study site is found out that Q. serrata community 34.4%, part of slope in valley is the distribution that dominant species is Q. serrata-Q. variabilis community 32.1%. And in valley showed Carpinus tschonoskii community 5.5%. And Sasa borealis was dominant species in the shrub layer. We couldn't supposed that the ecological succession sere of the study site, however we should do a long-term monitoring to investigate the changes of the ecological succession each plant community, According to the index of Shannon's diversity (unit: $400m^2$), community III was ranged from 1.0102 to 1.1013, community V was 0.9945, community II was ranged from 0.7913 to 1.1503, community IV was ranged from 0.8081 to 1.0749 and community I was 0.9273.

Vegetation Characteristics and Changes of Evergreen Broad-Leaved Forest in the Cheomchalsan(Mt.) at Jindo(Island) (진도 첨찰산 상록활엽수림의 식생 특성과 변화상)

  • Lee, Sang-Cheol;Kang, Hyun-Mi;Yu, Seung-Bong;Choi, Song-Hyun
    • Korean Journal of Environment and Ecology
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    • v.34 no.3
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    • pp.235-248
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    • 2020
  • The purpose of this study was to quantitatively analyze and investigate changes in the structural characteristics of the warm-temperate evergreen broad-leaved forest community in Mt. Cheomchalsan on Jindo Island. The Mt. Cheomchalsan has high conservation value because the representative warm temperate species such as Quercus acuta and Castanopsis sieboldii are distributed there. The community classification with TWINSPAN and DCA identified 4 communities: C. sieboldii community (I), C. sieboldii-Q. Salicina community (II), Q. acuta-C.sieboldii community (III), and deciduous broad-leaved trees-evergreen broad-leaved trees community (IV). According to the results of the mean importance percentage (MIP) analysis, C. sieboldii, Q. salicina, and Q. acuta were dominant species in the canopy layer, Camellia japonica, Ligustrum japonicum, and Cinnamomum yabunikkei were dominant in the understory layer, and Trachelospermum asiaticum, C. japonica, and C. sieboldii were dominant in the shrub layer. The comparison of the results of the diameter of breast height (DBH) analysis with the past data showed that the ratio of large-sized trees in the C. sieboldii and Q. acuta, which dominated the canopy layer, increased. However, there was no difference in the distribution of C. japonica and L. japonicum in the understory layer. In the future, it is necessary to generate a precision inhabiting vegetation map around the Natural Reserve to understand the actual habitation of evergreen broad-leaved trees and rezone the protective districts of evergreen broad-leaved trees forest with the watershed concept to preserve the evergreen broad-leaved forests of Mt. Cheomchalsan in Jindo.

Changes of Forest Soil and Herb Layer Composition by Group Breeding of Herons (백로(白鷺)와 왜가리의 집단번식(集團繁殖)에 의한 삼림토양(森林土壤) 및 초본층(草本層) 구조(構造)의 변화(變化))

  • Mun, Hyeong-Tae;Nam, Mi-Sook;Cho, Sam-Rae
    • Journal of Korean Society of Forest Science
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    • v.85 no.3
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    • pp.506-512
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    • 1996
  • Community structure and soil properties of larch forest which are used for breeding site by herons were studied at Apgogri, Hoengsung, Kangwon Province. This site has been protected as a Natural Monument(No. 248) from 1973, in Korea. Herons have used this habitat from February to October every year. In 1995, more than 700 herons were observed in this habitat. Most of the larches died due to group breeding of herons, and no larch saplings were found at forest floor. Nutrient contents of soil in this habitat were much higher than those in control plot(non-breeding site). This must be due to the addition of faeces from herons and of thin twigs and other organic materials from the canopy and bird nests. Species composition of herb layer in this habitat was quite different from that in control plot. Breeding site was dominated by Humulus japonicus, Persicaria fauriei, Persicaria perfoliata, Commelina communis, Chelidonium majus var. asiaticum, Digitaria sanguinalis, Bidens bipinnata. Dioscorea batatas which are indicator species of soil eutrophication. Similarity index of shrub and herb layer between the breeding site and control site was 0.36 and 0.07, respectively.

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