• Title/Summary/Keyword: SHP-1/2

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Home-ranges of Female Pipistrellus abramus (Chiroptera: Vespertilionidae) in Different Reproductive Stages Revealed by Radio-telemetry (원격무선추적을 이용한 집박쥐 암컷의 번식단계에 따른 행동권 분석)

  • Chung, Chul-Un;Han, Sang-Hoon;Kim, Sung-Dae;Lim, Chun-Woo;Kim, Sung-Chul;Kim, Chul-Young;Lee, Hwa-Jin;Kwon, Yong-Ho;Kim, Young-Chae;Lee, Chong-Il
    • Korean Journal of Environment and Ecology
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    • v.25 no.1
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    • pp.1-9
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    • 2011
  • In order to analyze the change in home ranges depending on the reproductive stage of Pipistrellus abramus, radio-tracking was carried out for a total of 9 individuals, 3 individuals each, by dividing stages into a pregnancy stage, lactation stage, and post-lactation stage from May to August 2009. For radio-telemetry, 0.38g transmitters, R2000 receivers and 3-element Yagi antennas were used. Pipistrellus abramus were captured using a double-stacked mist net and a harp-trap. Analysis of home ranges used a SHP File and ArcGIS 3.3 for GIS, and used a Kernel Home Range Method(KHR) and a Minimum Convex Polygon(MCP) Method for analysis. Home ranges at the pregnancy stage were MCP 100% $13.46{\pm}1.84ha$, MCP 95% $12.28{\pm}2.15ha$, KHR 50% $3.00{\pm}0.71ha$, and home ranges at the lactation stage were MCP 100% $8.13{\pm}0.23ha$, MCP 95% $7.73{\pm}0.63ha$, KHR 50%$1.84{\pm}1.05ha$. Home ranges at the post-lactation stage were MCP 100% $125.58{\pm}97.77ha$, MCP 95% $123.89{\pm}97.73ha$, KHR 50% $28.61{\pm}26.78ha$. As a result, home ranges of pipistrellus abramus showed a significant difference in all of the MCP 100%, MCP 95%, KHR 50% depending on reproductive stages, being largest in the post-lactation stage and smallest in the lactation stage.

Movements and Home-range of Mallards by GPS-Mobile based Telementary (WT-200) in Korea (야생동물위치추적기(WT-200)를 이용한 청둥오리의 이동거리 및 행동권 연구)

  • Kang, Tehan;Kim, Dal-Ho;Cho, Hae-Jin;Shin, Young-Un;Lee, Hansoo;Suh, Jae-Hwa;Hwang, Jongkyung
    • Korean Journal of Environment and Ecology
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    • v.28 no.6
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    • pp.642-649
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    • 2014
  • Mallard (Anas platyrhynchos) is the abundant winter visitor in South Korea. Mallard migrates long distances between Russian Siberia and Korea. This species prefers a rice paddy area as their winter habitat. We captured birds using cannon-net, and attached the GPS-Mobile phone based Telemetry(WT-200) on Seven Mallards in the winter of 2011~2013. We were monitored wintering home-range and movement distance. We analyzed the tracking location data using ArcGIS 9.0 and calculated Kernel Density Estimation (KDE) and Minimum Convex Polygon (MCP). The average home-range in the wintering ground by MCP was $118.8km^2$(SD=70.1, n=7)and the maximum home-rang was $221.8km^2$ and the minimum was $27.7km^2$. Extents of home-range by KDE were $60.0km^2$(KDE 90%), $23.0km^2$(KDE 70%) and $11.6km^2$(KDE 50%). Mallard moved an average of 19.4 km from start site(attach to WT-200 site), maximum moved was 33.2 km and minimum moved was 9.4 km. The average distance of 0.8 km between GPS fixed point(range 0.2~1.6 km), maximum moved was 19.7 km. Mallard moved a very short distance in wintering season and showed a very high water-dependent trends in wintering site.

Surface Micro-Climate Analysis Based on Urban Morphological Characteristics: Temperature Deviation Estimation and Evaluation (도시의 지표형태학적 특성에 기반한 지면미기후 분석: 기온추정 및 평가)

  • Yi, Chaeyeon;An, Seung Man;Kim, KyuRang;Kwon, Hyuk-gi;Min, Jae-Sik
    • Atmosphere
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    • v.26 no.3
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    • pp.445-459
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    • 2016
  • Air temperature deviation (ATD) is one of major indicators to represent spatial distribution of urban heat island (UHI), which is induced from the urbanization. The purpose of this study is to evaluate the accuracy of air temperature deviation about Climate Analysis Seoul (CAS) workbench, which had developed by National Institute Meteorological Science and TU Berlin. Comparison and correlation analysis for CAS ATD including meso-scale air temperature deviation, local-scale air temperature deviation, total air temperature deviation, surface heat flux deviation, cold air production deviation among meso-scale numerical modelling variable in 'Seoul Region', micro-scale numerical modelling in 'Detail Region', and CAS workbench variable using observation data in ground stations. Comparison between night time OBS ATD and CAS ATD show that have most close values. Most of observations ($dT_{max}$ and $dT_{min}$) have highly positive ($dT_{SHP}$, $dT_{CA}$, MD, TD, $f_{BS}$, $f_{US}$, $f_{WS}$, $h_B$) and negative ($f_{VS}$, $f_{TV}$, $h_V$, Z) correlations. However, CAS workbench needs further improvement of both observational framework and analytical framework to resolve the problems; (1) night time OBS ATD of has closer values in compare with at high rise mountain area and (2) correlations are very dependable to meteorological scale.

Genes profile related to modulation of natural killer cell activity induced by electroacupuncture (전침이 자연살해세포 활성에 미치는 유전자 발현 profile에 대한 연구)

  • Choi, Gi-soon;No, Sam-woong;Oh, Sang-deog;Bae, Hyun-su;Ahn, Hyun-jong;Ha, Yoon-mun;Kim, Kwang-ho;Min, Byung-il
    • Journal of Acupuncture Research
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    • v.19 no.6
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    • pp.111-124
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    • 2002
  • A line of study reported that electroacupuncture(EA) modulate natural killer cell(NK Cell) activities. One report suggested that EA enhanced splenic interferon-gamma($IFN-{\gamma}$), interleukin-2(IL-2), and NK cell activity in Sprague-Dawley rats. Another study suggested that $IFN-{\gamma}$ mediates the up-regulation of NK cell activity, and endogenous ${\beta}$-endorphin secretion also play a role in the up-regulation of NK cell activity induced by EA stimulation. In order to better understand the molecular regulation underlying the activation of NK cell induced by EA, we have utilized cDNA microarray to elucidate how EA alters program of gene expression of spleen in rats. First, we divided three groups, group I was EA group treated with EA in restriction holder, group II was sham group with only holder stress, and last group III was control group with no treatment. We measured NK cell activity after EA stimulation three times for 2 days using $^{51}Cr$ release assay. Second, Biotin-labeled cDNA probes synthesized from EA group and sham group, were competitively hybridized to the microarray that contained variable genes. Such high-throughput screening has identified a number of EA-responsive gene candidates. Of these, we found that EA induced a subset of genes of genes that functionally could modulatory effects on NK cell activity. Genes(vascular cell adhesion molecule-1, protein-tyrosine kinase, CD94 mRNA) related to boost NK cell activity, were increased by EA And, genes(protein-tyrosine-phospatase mRNA, protein-tyrosine phosphatase(SHP-1) mRNA) related to inhibit NK cell activity, were decreased by EA. These EA-responsive genes may provide key insights from which to understand mechanisms of activation of NK cell induced by EA.

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A Study on Status of Utilization and The Related Factors of Primary Medical Care in a Rural Area (일부 농촌지역의 일차의료이용실태와 그 관련요인에 관한 연구)

  • Wie, Cha-Hyung
    • Journal of agricultural medicine and community health
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    • v.20 no.2
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    • pp.157-168
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    • 1995
  • This study was carried out, through analyzing the annual reports(year of 1973-1993) on health status of Su Dong-Myun, and specific survey data of 332 households(Su Dong-Myun 209, Byul Nae-Myun 123), located in Nam Yang Ju-Si, Kyung Gi-Do, from July 20 to July 31, 1995, to find out more effective means for primary medical care in a rural area. The results were as fellows : 1. Number of population in Su Dong-Myun was 5,419 in 1973, 4,591(the lowest) in 1987 and 5,707 in 1995. In the composition rate of population, "0-14" of age group showed markedly decreasing tendency from 43.1% in 1975, to 19.1% in 1995, however "65 and over" markedly in creasing tendency form 5.3% in 1975 to 9.8% in 1995. 2. Annual utilization rate per 1,000 inhabitants in Su Dong-Myun showed markedly increasing tendency from 1973 to 1977 such as 343 in 1973, 540 in 1975, 900 in 1977. However, since 1979, the rate showed rapidly decreasing tendency, such as 846 in 1979, 519 in 1985, 190 in 1991 and 1993. 3. The morbid household rate per year was 53.6% of respondents and the rate per 15 days was 48.2%. In disease classification rate of morbid household per year, Arthralgia & Neuralgia was the highest rate(33.9%) and gastro-intestinal disorder(19.3%), Cough(11,9%), Hypertension(7.8%), Accident(3.2%) in next order. 4. In the utilizing facilities for Primary Medical Care, Medical facilities was showed the highest rate(58.1% of respondents) and Pharmacy and Drug Shp(33.1%), Tradition Method(4.0%) in next order. In the Medical facilities, General private clinic was showed the highest rate(34.3%) and specific private Clinic(22.3%), Hospital(19.0%), Health (Sub)center(16.3%), Nurse practitioner (3.3%), Oriental hospital and clinic(2.7%) in next order. 5. Experience rate, utilizing health subcenter was 51.8% of the respondents, and it was 55.0% in Su Dong-Myun and 46.3% in Byul Nae-Myun. In utilization times of health subcenter, times-rate showed next orders such as 1-2 times/6months(31.6%), 1-2 times/year (22.1%), 1-2 times/months(19.2%), 1-2 times/3months(15.6%). 6. In objectives, visiting Health Subcenter, Medical Care was the highest rate(59.8% of the respondents) and health control(23.3%) was in next order. In Medical Care, Primary Care by general physician was higher rate(51.1%) almost all. In the Health control, Immunization too was high rate(18.0%) in health control activities. 7. The reasons rate, utilizing health subcenter showed next order, such as distance to Medical facilities(33.0% of the respondents), Medical Cost(28.1%), Simple process of consultation (10.8%), Effectiveness of cure(7.6%), Function of primary medical care(7.0%) and Attitude of physician(6.5%). 8. In the affecting factors to utilization of primary medical facilities, medical needs was showed the highest rate(29.5% of the respondents) and medical cost(15.4%), distance to medical facilities(14.2%), traffic vehicle(14.2%) and farm work(6.9%) in next order. 9. In the priority between 'daily farm work,' and 'primary medical care', only 46.4% of respondents answered that primary health care is more important than the daily farm work The 22.6% of respondents answered 'daily farm work', and the 12.3% answered 'the equal of the both'. 10. In the criterion of medical facilities choice, medical knowledge and technical quality was showed the highest rate(56.3%), distance or time to medical facilities(10.9%), sincerity and kindness of physician(9.4%), medical cost(8.7%) and traffic vehicle(6.5%) in next order 11. In the advise for improvement of health subcenter function, the 36.1% of respondents answered that 'enforcement of medical personnel and equipment' was required, and then 'improved medical technology'(25.5%), 'good attitude of physician'(14.9%), 'improved medical system'(13.3%), 'enforced drug'(6.7%) in next order. 12. The study on affecting factors to utilization of primary medical facilities was very difficult subject to systematize the analyzed results, due to a prejudice of protocol planner, surveyer and respondent, and variety and overlapping of subject matter.

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The Preparation of porous ceramic material from aluminum waste dross (알루미늄 廢드로스를 活用한 세라믹 多孔體의 製造)

  • Kim, Ki-Seok;Park, Jay-Hyun;Park, Jai-Koo
    • Resources Recycling
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    • v.14 no.2
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    • pp.19-27
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    • 2005
  • The recycling possibility of aluminum waste dross(AWD) as a ceramic raw material of porous light-weight material was examined. A aluminum waste dross was washed 4-7 consecutive times and roasted at 900$^{\circ}% for 1hour as pre-treatments. The properties of the pre-treatment of aluminum waste dross was investigated. It was conformed by XRD result that the spinel crystalline was grown in AWD, after roasting. After the roasted AWD was ground in aqueous state, the sodium hexaphosphate(SHP) as a dispersant which is used for stabilizing the concentrated slurry was added to the AWD slurry. The porous material was prepared by slurry foaming method with surfactant at room temperature. The foamed slurry volumes were 2 and 3 times of the original slurry volume. The properties of porous material with extended volume of 3 times was following: the porosity was about 84%, bulk density was 0.59 g/cm$^3$, the range of pore was from 50 ${\mu}m$ to 500 ${\mu}m$ and mean pore size was about 200 ${\mu}m$. AWD porous material was sintered at 1150$^{\circ}C-1250$^{\circ}C. It was colcluded that AWD was sintered well at 1200$^{\circ}C from material surface observation by SEM.

Study on the Home-range and Winter Habitat Pintail using the Wild-Tracker (WT-300) in Korea (WT-300을 이용한 월동기 고방오리(Anas acuta)의 행동권 및 서식지 이용연구)

  • Jung, Sang-Min;Shin, Man-Seok;Cho, Hae-jin;Han, Seung-Woo;Son, Han-Mo;Kim, Jeong Won;Kang, Sung-Il;Lee, Han-soo;Oh, Hong-Shik
    • Korean Journal of Environment and Ecology
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    • v.33 no.1
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    • pp.1-8
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    • 2019
  • Pintail (Anas acuta) is the major wintering bird in South Korea and known as a major mediator of the highly pathogenic avian influenza (HPAI). Pintail migrates long distances between Russian Siberia and Korea. This species prefers a rice paddy area as their winter habitat. The purpose of this study is to provide the data necessary for the conservation and management of bird habitats in Korea by understanding the wintering home-range and habitat of pintail in Korea. We captured six pintails using a cannon-net in the winter of 2015 and attached the GPS-mobile phone based telemetry (WT-300) on them to study the wintering home-range and wintering habitat. We analyzed the tracking location data using ArcGIS 9.0 Animal Movement Extension and calculated Kernel Density Estimation (KDE) and Minimum Convex Polygon (MCP). The average home-range in the wintering ground analyzed by MCP was $677.3km^2$ (SD=130.2, n=6) while the maximum and minimum were $847.7km^2$ and $467.5km^2$, respectively. Extents of home-range analyzed by KDE were $194.7km^2$ (KDE 90%), $77.4km^2$ (KDE 70%), and $35.3km^2$ (KDE 50%). The pintails mostly used both sea and paddy field as habitat in the winter season and utilized paddy fields more during the nighttime and than the daytime. We concluded that the home-range and habitat of pintails in the winter could be used as the reference data for the preservation of species, management of habitats, and coping with a breakout of HPAI.

Monitoring trafficking and expression of hemagglutinin-tagged transient receptor potential melastatin 4 channel in mammalian cells

  • Eun Mi Hwang;Bo Hyun Lee;Eun Hye Byun;Soomin Lee;Dawon Kang;Dong Kun Lee;Min Seok Song;Seong-Geun Hong
    • The Korean Journal of Physiology and Pharmacology
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    • v.27 no.4
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    • pp.417-426
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    • 2023
  • The TRPM4 gene encodes a Ca2+-activated monovalent cation channel called transient receptor potential melastatin 4 (TRPM4) that is expressed in various tissues. Dysregulation or abnormal expression of TRPM4 has been linked to a range of diseases. We introduced the hemagglutinin (HA) tag into the extracellular S6 loop of TRPM4, resulting in an HA-tagged version called TRPM4-HA. This TRPM4-HA was developed to investigate the purification, localization, and function of TRPM4 in different physiological and pathological conditions. TRPM4-HA was successfully expressed in the intact cell membrane and exhibited similar electrophysiological properties, such as the current-voltage relationship, rapid desensitization, and current size, compared to the wild-type TRPM4. The presence of the TRPM4 inhibitor 9-phenanthrol did not affect these properties. Furthermore, a wound-healing assay showed that TRPM4-HA induced cell proliferation and migration, similar to the native TRPM4. Co-expression of protein tyrosine phosphatase, non-receptor type 6 (PTPN6 or SHP1) with TRPM4-HA led to the translocation of TRPM4-HA to the cytosol. To investigate the interaction between PTPN6 and tyrosine residues of TRPM4 in enhancing channel activity, we generated four mutants in which tyrosine (Y) residues were substituted with phenylalanine (F) at the N-terminus of TRPM4. The YF mutants displayed properties and functions similar to TRPM4-HA, except for the Y256F mutant, which showed resistance to 9-phenanthrol, suggesting that Y256 may be involved in the binding site for 9-phenanthrol. Overall, the creation of HA-tagged TRPM4 provides researchers with a valuable tool to study the role of TRPM4 in different conditions and its potential interactions with other proteins, such as PTPN6.

Interzonal Comparative Analysis of the Wintering Habitat of Spot-billed Duck (Anas poecilorhyncha) (흰뺨검둥오리의 지역간 월동서식지이용 비교분석)

  • Hwang, Jong-Kyeong;Shin, Man-Seok;Kang, Young-Myong;Yoom, Hachung;Choi, Jida;Jeong, Wooseog;Lee, Jun-Woo
    • Korean Journal of Environment and Ecology
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    • v.30 no.4
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    • pp.676-683
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    • 2016
  • This study was conducted using the Wild -Tracker (WT-300, GPS-Mobile Phone Based Telemetry KoEco) to understand the habitats of the spot-billed duck wintering in urban and rural areas and provide the results as the basic data for the protection and management of the habitats of the waterbirds in Korea. Study areas consisted of the Anseong stream in Gyeonggi-do and the Sansu reservoir in Haenam. Five spot-billed ducks were captured by region, and we attached Wild-Tracker to each of the spot-billed ducks. We analyzed the tracking location data using ArcGIS 9.x and calculated Kernel Density Estimation (KDE) and Minimum Convex Polygon (MCP). The average home-range measured by MCP was $250.8km^2$(SD=195.3, n=5) in Anseong and was $89.1km^2$ (SD=69.6, n=5) in Haenam. 50% home-range measured by KDE was $21.8km^2$ (SD=26.9, n=5) in Anseong and $3.5km^2$ (SD=2.2, n=5) in Haenam, indicating a narrow home range in Haenam. During the winter season, both wetland and paddy field were mostly used as habitats in Anseong and Haenam. While the paddy field utilization rate was high in the daytime in Haenam, it was high in the nighttime in Anseong. By late winter, Haenam's day time paddy field utilization rate and Anseong's night time paddy field utilization rate increased.

Effects of Wood Particles and Steel Wire Compositions on Physical and Mechanical Properties of the Boards (목재(木材)파아티클과 철선(鐵線) 복합체(複合體)가 보오드의 물리적(物理的) 및 기계적(機械的) 성질(性質)에 미치는 영향(影響))

  • Park, Heon;Lee, Pill-Woo
    • Journal of the Korean Wood Science and Technology
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    • v.14 no.1
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    • pp.3-44
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    • 1986
  • In order to obtain the basic physical and mechanical properties of steel wire reinforced particleboard, particleboards were formed with large particles through 2.11 mm (12 meshes) and retained on 1.27mm (20 meshes) sieves and small particles through 1.27mm (20 meshes) and retained on 0.42mm (60 meshes) sieves from the plywood mill wastes of meranti (Shorea spp.) in the form of pallmanchips, applying urea-formaldehyde resin as an adhesive on the particle surface in 10 percent on the oven dried weight of particles, and arranging steel wires of 1mm in diameter 5,10,15,20, and 25mm in longitudinal and transverse direction with crossing in the mid of the board depth in single layer boards, 10mm in longitudinal or transverse direction without crossing in two layers and 10mm in longitudinal and transverse directions with and without crossing in three steel wire layers boards. The stepwise 9-minutes-multi-pressing schedule in 5 minutes at 35 kgf/$cm^2$, 2.5 minutes at 25 kgf/$cm^2$. and 1.5 minutes at 15 kgf/$cm^2$ was applied for $300{\times}200{\times}13$mm board at the temperature of 160$^{\circ}C$ in a hot press. Specific gravity, thickness swelling, bending properties of modulus of rupture (MOR), modulus of elasticity(MOE), work to proportional limit, and work to ultimate load, internal bond (IB), and screw holding power(SHP) of the reinforced boards were analyzed on the wire openings and wire layers. The results obtained are summarized as follows; 1) In specific gravity, particleboards with large particles and small particles had higher value with more steel wire placements and more steel layers composition, 2) Particleboards with large particles in accordance with more steel wire liners composition gave very poor thickness swelling. 3) The mechanical properties of particleboards formed with large or small particles were reinforced with more steel wire layers. Therefore, bending strength was improved in modulus of rupture, modulus of elasticity, and work to ultimate load. Especiallv, particleboards with two or three steel wire layers showed the tension lamination effect when the steels in lower steel wire layer were oriented parallel to the board length. 4) The modulus of rupture, modulus of elasticity, and work to ultimate load in bending varied with opening area, distance of lengthwise wires multipled by distance of transverse wires. Particleboards formed with large particles resulted in higher value in modulus of rupture with 1.5-3 $cm^2$ opening area, 1-2cm distance between transverse wires, and 1.5-2.5cm distance between lengthwise wires. Particle boards formed with small particles showed higher value with 0.5-1.5$cm^2$ or 3.75-6.25 $cm^2$ opening area, 0.5 or 2.5cm distance between transverse wires. 5) In modulus of elasticity, particleboards formed with large particles with one steel wire layer suggested higher value with 5-3$cm^2$ opening area, 1-2.5cm distance between transverse wires and also 1-2.5 cm distance between lengthwise wires. Particleboards formed with small particles showed higher value with 0.75-1.25$cm^2$ or 3-6.25$cm^2$ opening area and 0.5 or 2.5cm distance between transverse wires. 6) Particleboards formed with large particles gaved higher value in work to ultimate load with 1-3$cm^2$ opening area. Particleboards formed with small particles showed increasing tendancy with decreasing opening area. 7) In internal bond and screw holding power, particleboards formed with large particles had increasing value in two and three steel wire layers compositions, but particleboards formed with small particles showed no difference. Particleboards formed with large particles containing one steel wire layer showed no difference in internal bond and screw holding power, and particleboards formed with small panicles containing one steel wire layer resulted in increasing value in internal bond and decreasing value in screw holding power in accordance with increase in opening area.

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