• Plant Biotechnology Reports
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• v.2 no.1
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• pp.13-20
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• 2008

Potato (Solanum tuberosum L.), one of the most important food crops, is susceptible to a number of devastating fungal pathogens in addition to bacterial and other pathogens. Producing disease-resistant cultivars has been an effective and useful strategy to combat the attack of pathogens. Potato was transformed with Agrobacterium tumefaciens strain EHA101 harboring chitinase, (ChiC) isolated from Streptomyces griseus strain HUT 6037 and bialaphos resistance (bar) genes in a binary plasmid vector, pEKH1. Polymerase chain reaction (PCR) analysis revealed that the ChiC and bar genes are integrated into the genome of transgenic plants. Different insertion sites of the transgenes (one to six sites for ChiC and three to seven for bar) were indicated by Southern blot analysis of genomic DNA from the transgenic plants. Expression of the ChiC gene at the messenger RNA (mRNA) level was confirmed by Northern blot analysis and that of the bar gene by herbicide resistance assay. The results obviously confirmed that the ChiC and bar genes are successfully integrated and expressed into the genome, resulting in the production of bialaphos-resistant transgenic plants. Disease-resistance assay of the in vitro and greenhouse-grown transgenic plants demonstrated enhanced resistance against the fungal pathogen Alternaria solani (causal agent of early blight).

• Advances in aircraft and spacecraft science
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• v.7 no.3
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• pp.203-214
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• 2020

In many applications like the aircraft or the rockets/missiles, the flow from a nozzle needs to be expanded suddenly in an enlarged duct of larger diameter. The enlarged duct is provided after the nozzle to maximize the thrust created by the flow from the nozzle. When the fluid is suddenly expanded in an enlarged duct, the base pressure is generally lower than the atmospheric pressure, which results in base drag. The objective of this research work is to optimize the length to diameter (L/D) ratio of the enlarged duct using the CFD analysis in the flow field from the supersonic nozzle. The flow from the nozzle drained in an enlarged duct, the thrust, and the base pressure are studied. The Mach numbers for the study were 1.5, 2.0 and 2.5. The nozzle pressure ratios (NPR) of the study were 2, 5 and 8. The L/D ratios of the study were 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10. Based on the results, it is concluded that the L/D ratio should be increased to an optimum value to reattach the flow to an enlarged duct and to increase the thrust. The supersonic suddenly expanded flow field is wave dominant, and the results cannot be generalized. The optimized L/D ratios for various combinations of flow and geometrical parameters are given in the conclusion section.

• Journal of Plant Biotechnology
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• v.49 no.1
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• pp.15-29
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• 2022

Cyclophilins (CYPs) are highly conserved ubiquitous proteins belong to the peptidyl prolyl cis/trans isomerase (PPIase) superfamily. These proteins are present in a wide range of organisms; they contain a highly conserved peptidyl-prolyl cis/trans isomerase domain. A comprehensive database survey identified a total of 35 genes localized in all cellular compartments of Solanum lycopersicum L., but largely in the cytosol. Sequence alignment and conserved motif analyses of the SlCYP proteins revealed a highly conserved CLD motif. Evolutionary analysis predicted the clustering of a large number of gene pairs with high sequence similarity. Expression analysis using the RNA-Seq data showed that the majority of the SlCYP genes were highly expressed in mature leaves and blooming flowers, compared with their expression in other organs. This study provides a basis for the functional characterization of individual CYP genes in the future to elucidate their role(s) in protein refolding and long-distance signaling in tomatoes and in plant biology, in general.

• Genomics & Informatics
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• v.19 no.2
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• pp.16.1-16.14
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• 2021

Even in the current age of advanced medicine, the prognosis of malignant peritoneal mesothelioma (MPM) remains abysmal. Molecular mechanisms responsible for the initiation and progression of MPM are still largely not understood. Adopting an integrated bioinformatics approach, this study aims to identify the key genes and pathways responsible for MPM. Genes that are differentially expressed in MPM in comparison with the peritoneum of healthy controls have been identified by analyzing a microarray gene expression dataset. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses of these differentially expressed genes (DEG) were conducted to gain a better insight. A protein-protein interaction (PPI) network of the proteins encoded by the DEGs was constructed using STRING and hub genes were detected analyzing this network. Next, the transcription factors and miRNAs that have possible regulatory roles on the hub genes were detected. Finally, survival analyses based on the hub genes were conducted using the GEPIA2 web server. Six hundred six genes were found to be differentially expressed in MPM; 133 are upregulated and 473 are downregulated. Analyzing the STRING generated PPI network, six dense modules and 12 hub genes were identified. Fifteen transcription factors and 10 miRNAs were identified to have the most extensive regulatory functions on the DEGs. Through bioinformatics analyses, this work provides an insight into the potential genes and pathways involved in MPM.

• Advances in aircraft and spacecraft science
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• v.9 no.2
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• pp.131-148
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• 2022

The paper presents the surface-modified NACA 2412 airfoil performance with variable cavity characteristics such as size, shape and orientation, by numerically investigated with the pre-validation study. The study attempts to improve the airfoil aerodynamic performance at 30 m/s with a variable angle of attack (AOA) ranging from 0° to 20° under Reynolds number (R_e) 4.4×10⁵. Through passive surface control techniques, a boundary layer control strategy has been enhanced to improve flow performance. An intense background survey has been carried out over the modifier orientation, shape, and numbers to differentiate the sub-critical and post-critical flow regimes. The wall-bounded flows along with its governing equations are investigated using Reynolds Average Navier Strokes (RANS) solver coupled with one-equational transport Spalart Allmaras model. It was observed that the aerodynamic efficiency of cavity airfoil had been improved by enhancing maximum lift to drag ratio ((l/d) max) with delayed flow separation by keeping the flow attached beyond 0.25C even at a higher angle of attack. Detailed investigation on the cavity distribution pattern reveals that cavity depth and width are essential in degrading the early flow separation characteristics. In this study, overall general performance comparison, all the cavity airfoil models have delayed stalling compared to the original airfoil.

• Advances in aircraft and spacecraft science
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• v.9 no.2
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• pp.119-130
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• 2022

Supersonic projectiles like rockets, missiles, or aircraft find various applications in the field of defense. The shape of the wings is mainly designed as wedge shape or delta wings for supersonic vehicles. The study of supersonic flows over the wedges and flat plate delta wings around the large scale of incidence angle is considered in the supersonic projectile. In the present paper, the prime attention is to study the pressure at the nose of the plane wedge over the various Mach number and the various angles of incidence. Ghosh piston theory is used to obtain the pressure distribution analytically, and the results are compared with CFD analysis results. The wedge angle and Mach number are the parameters considered for the research work. The range of wedge angle is 50 to 250, and Mach number is 1.5 to 4.0 are considered for the current research work. The analytical results show excellent agreement with the CFD results. The results show that both the parameters wedge angle and Mach number are influential parameters to vary the static pressure. The static pressure increases with an increase in Mach number and wedge angle.

• The Journal of Asian Finance, Economics and Business
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• v.9 no.3
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• pp.149-157
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• 2022

The primary goal of this study is to investigate the impact of social capital on consumer attitudinal evaluations. We propose a hypothetical receptacle, thereby applying a mediation framework because of social capital. We expect that increasing social capital would improve brand image and consumer-company (C-C) identification, resulting in positive in-role and extra-role customer behavior toward the brand. Data was collected from 425 respondents primarily from Karachi's five zones (East, West, North, South, and Central) and analyzed using confirmatory component analysis and structural equation design. The findings showed that social capital had a positive and significant relationship with customer extra-role behavior, as well as two mediators, brand image and consumer business identity. Furthermore, both mediators have a significant impact on both in-role and extra-role behavior. However, there is no evidence that social capital has a direct impact on in-role behavior. This study will help businesses in gaining a competitive advantage by concentrating on social capital to improve their brand image and customer relationship.

• Asia pacific journal of information systems
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• v.31 no.1
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• pp.17-42
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• 2021

This study examines the adoption of subscription-based video on demand (SVOD) streaming services among consumers. Primarily, we explore the moderating effect of the two models of streaming services, standalone streaming services and bundled streaming services, on the users' adoption. We employ the Unified Theory of Acceptance and Use of Technology (UTAUT2) model in this study. We utilize the data collected from 337 Indian respondents and find that all constructs of the UTAUT2 model act as motivators of adoption. Gender, age, and experience of the respondent also play a moderating role in the adoption of streaming services. We also find that providing bundled streaming service positively moderates price-value and hedonic motivation of adoption. The study is perhaps the first of its kind that aims to understand the motivations for adoption of SVOD services, particularly in the Indian context, which has the fastest-growing base of internet users in the world.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.11
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• pp.5613-5617
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• 2012

Gallbladder carcinoma (GBC) is the commonest cancer of the biliary tree and the most frequent cause of death from biliary malignancies. The incidence of GBC shows prominent geographic, age, race, and gender-related differences and is 4-7 times higher in patients with gallstones. This prompted us to study the clinicopathological aspects of the disease and the incidence of gallstones in gallbladder carcinoma patients, in this part of India. In this, combined retrospective (Jan 2004-March 2010) and prospective study (April 2010-Dec 2011) of eight years, 198 patients of gallbladder carcinoma (50 males and 148 females), (range 28-82 years; mean 55 years) were studied. Most of the patients were poor and presented with abdominal pain and mass, with abnormal lab parameters. Gallstones were present in 86% of patients. Surgical exploration was performed in 130, with gallbladder resection in 60 (including 7 incidental GBC). Adenocarcinoma (87.7%) was the commonest histological type. The study indicates that GBC is common in our scenario. It is a disease of elderly females, has a strong association with gallstones and every cholecystectomy specimen should be examined histopathologically.

• Korean journal of applied entomology
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• v.40 no.1
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• pp.5-13
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• 2001

Two unrecorded species of Heteroderinae, Clobodera hypolysi Ogawa, Ohshima and Ichinohe, 1983 and Sarisodera hydrophila Wouts and Sher, 1971 from Korea were firstly reported. The Korean specimens well correspond with the original description of Globodera hypolysi except stylet length of the juveniles, slightly shorter (21~24 vs. 24~25 $\mu$m) and shorter distance from the anus to the fenestra in cyst (22~50 vs. 25~80$\mu$m). The measurements of Korean specimen of Sarisodera hydrophila are slightly short compared with the type species; Female body length 367.2~597.6$\mu$m vs 359~688$\mu$m; Stylet length 37~44$\mu$m vs 38~46$\mu$m; Male body length 662~11987m vs 579~1405$\mu$m; stylet length 37~43.8$\mu$m vs 38~46$\mu$m. Juvnile body length 460~536$\mu$m vs 466~5587m. stylet length 32~387m vs 39~43$\mu$m.