• Asian-Australasian Journal of Animal Sciences
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• v.31 no.8
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• pp.1285-1290
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• 2018

Objective: This study shows the effects of dietary fiber levels on cecal microbiota composition in geese at day 70 according to pyrosequencing of the 16S ribosomal RNA gene. Methods: A total of 468 1-day-old healthy male Yangzhou goslings with similar body weight were randomly divided into 3 groups with 6 replicates per group and 26 geese per replicate. Geese were fed diets with fiber levels of 2.5% (low fiber level diet, Group I) and 6.1% (Group III) during days 1-70, respectively, or 4.3% for days 1-28 and 6.1% for days 29-70 (Group II). Results: Low fiber level diet decreased body weight, average daily gain during, increased lower feed conversation rate of geese during day 1 to 70 (p<0.05). Low fiber level diet decreased the total operational taxonomic units, Chao1 index and Shannon index, whereas increased the Simpson index of cecal microbiota in geese at day 70. Low fiber level diet decreased the relative abundance of Bacteroidetes, Firmicutes, Bacteroides, and Paraprevotella in cecum of geese at day 70. The similarity of cecal microbiota between low fiber level diet group and other groups was smaller. Conclusion: This study indicates that the low fiber level diet decreased diversity of microbiota, and relative abundance of some beneficial microbiota in cecum of geese at day 70, implying that the low fiber level diet has negative influence on performance by altering the diversity and population of cecal microbiota in geese.

• Korean Journal of Ecology and Environment
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• v.45 no.1
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• pp.72-81
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• 2012

We investigated the effects of acid mine drainage (AMD) from abandoned coal mines on benthic macroinvertebrate communities in the upper reaches of the Nakdong River from May to October in 2009. Qualitative and quantitative sampling (Surber sampler: $50{\times}50$ cm; mesh size, 0.2 mm) was conducted at 7 study sites and 3 control sites in the study area. We thus sampled 117 species belonging to 53 families, 15 orders, 6 classes, and 5 phyla; the Ephemeroptera, Plecoptera, and Trichoptera group (EPT-group) represented the majority of the benthic macroinvertebrate community (71 species; 64.5%). In the quantitative sampling, a total of 11,575 individuals belonging to 58 species of benthic maroinvertebrates were sampled from the study sites (Sites 1-7), whereas 2,844 individuals belonging to 79 species were sampled from the control sites (Sites A-C). Tolerant species such as oligochaetes, $Epeorus$ $pellucidus$, $Baetis$ $fuscatus$, Hydropsychidae species, and Chironomidae species were predominant in the study sites. The community indices for the study sites, such as MacNaughton's dominance index (DI) (mean${\pm}$SD, $0.52{\pm}0.21$; range, 0.33-0.85) and the Shannon diversity index ($H^{\prime}$) ($2.06{\pm}0.60$; 1.06-2.57), were different from those for the control sites (DI: $0.29{\pm}0.07$, 0.22-0.35; $H^{\prime}$: $3.13{\pm}0.14$; 3.03-3.30). In the study sites, shredders and scrapers were scarce, whereas gathering-collectors (mainly Chironomidae species) were relatively abundant, as were clingers and burrowers. The detrended correspondence analysis (DCA) and similarity analyses showed that benthic macroinvertebrate communities in the study sites were clearly separated from those of the control sites, with the greatest dissimilarity being noted at the uppermost study site (Site 1), which is located close to an abandoned coal mine. The Korean saprobic index (KSI) and the ecological score using benthic macroinvertebrates (ESB) showed that the uppermost study site (Site 1) was ${\alpha}$-mesosaprobic or heavily polluted, whereas other study sites were in a fair or relatively good condition.

• Journal of Microbiology and Biotechnology
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• v.12 no.3
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• pp.382-388
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• 2002

In order to assess the effects of organic fertilizer on soil microbial community structure and diversity in the greenhouse fields, fatty acid methyl ester (FAME) was analyzed by the MIDI (Microbial ID, Inc., Newark, DE, U.S.A.) system and enumerations were performed. In relation to bacterial division of each sample, low GC Gram-positive bacteria were predominant among bacteria cultured on aerobic bacteria media. On the other hand, alpha subdivision was predominant on proteobacteria of control and OM (organic matter) 1 treated plot, and Flavobacterium spp. existed in OM2 plot on crystal violet media of all samples. Shannon-weaver Index (H) of OM1 plot varied most by 1.9 and 5.0 among bacteria cultured on aerobic bacteria media and crystal violet media, respectively. Our results revealed that addition of the organic wastes to soil led to a highly diverse microbial community, but the excessive amounts of organic and mineral fertilizer applied in the greenhouse fields produced excess nutrients in soil and led to simplification on bacterial populations.

• Korean Journal of Environment and Ecology
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• v.18 no.3
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• pp.279-287
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• 2004

To investigate the vegetation structure of the section of Nakdong-jeongmaek from Gajisan to Neungdongsan, fifty plots of 100$m^2$ at ten locations were set up. In accordance with the requirements of DCA(detrended correspondance analysis), which is an ordination technique, the sites were chosen contiguously with each other. The vegetation structure analysis showed Quercus dentata, Pinus densiflora, Q. mongolica to be the major species at canopy layer, Rhododendron schlippenbachii at the layer below the canopy and Tripterygium regelii at the shrub level. Shannon's diversity index was 1.5315～2.4005 per unit area of 500$m^2$. It appeared that the Quercus dentata forest of this ridge section of Nakdong-jeongmaek is about 30 or 40 years old.

• Journal of Environmental Science International
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• v.11 no.11
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• pp.1149-1156
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• 2002

The ecological characteristics of Dolbae trees, a valuable genetic resource for native pyrus fruit trees, were investigated at four naturally inhabited areas on Mount Kaya, Gyeongsangnam-Do. Most Dolbae trees were found on the southwest side of an old hiking path to a mountain valley at altitudes of 610∼670 m. The surrounding flora consisted of 46 families, 69 genera, 75 species, and 10 varieties. The natural habitat of the Dolbae trees was basically a deciduous broad-leaved forest with a predominance of Quercus serrata and ligneous plants(xylophytes) from the Fagaceae and Betulaceae families. The habitat was also found to be in a second transition resulting from forest disturbance and exhibited a degree of diversity of 2.901-5.065, based on Shannon-Weiner's index. Examination of the Dolbae trees found in Mount Kaya revealed diameter breast heights(DBHs) ranging from 10 to 60 cm, including six old and large Dolbae trees with a DBH of 50∼60 cm, which will be particularly valuable as a genetic resource. Accordingly, the current results suggest that the study areas are a secondary forest in a typical mid-temperate zone resulting from forest damage about 10 years earlier.

• Journal of Forest and Environmental Science
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• v.31 no.2
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• pp.126-135
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• 2015

This study was conducted to evaluate the ecological characteristics of forest cover types which were classified by cluster analysis in the natural forest of Sobaeksan on the basis of the vegetation data from the point-quarter sampling method. Recognized forest cover types were 1) Mixed mesophytic forest, 2) Taxus cuspidata forest, 3) Fraxinus rhynchophylla-Quercus mongolica forest, 4) Betula ermanii forest, 5) Pinus densiflora forest, 6) Quercus mongolica mixed forest, and 7) Quercus mongolica pure forest. For those of classified types, the species composition was expressed by importance value (IV) to describe the community floristically. The species diversity was quantified using the Shannon's diversity index. The results showed that the forest cover types were characteristically different from one another in growing species and compositional rates, depending upon the type which was formed by a number of similar vegetational sample points. Species diversity indices (H') of total and overstory both were the highest in the mixed mesophytic forest (3.530 and 2.880, respectively), and lowest in the Q. mongolica pure forest (2.122 and 0.000, respectively) with only one canopy species. The highest species diversity in the mixed mesophytic forest may due to the relatively high species richness and evenness in the forest cover types. The description on ecological characteristics were suggested to understand the formation and development of forest cover types in this study area.

• Journal of Environmental Science International
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• v.20 no.6
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• pp.701-710
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• 2011

The purpose of this study is to investigate the vegetation structure and ecological restoration of disturbed forest due to artificial plant. To this end, 12 plots were set up and surveyed. The result analyzed considering mean importance percentage(M.I.P) showed that the types were divided into three groups which are artificial planted forest type(three plots), natural forest-artificial planted forest type(four plots), natural forest type(five plots). Dominant proportion of artificial planted species were as follows: artificial planted forest type was over 60%, natural forest-artificial planted forest types were 14~49%. The range of Shannon's index of all associations was from 0.7131 to 0.7771(natural forest-artificial planted forest > natural forest > artificial planted forest). Also we suggested restoration method of vegetation for ecological value as follow: Control of density considering step and Remove of Pinus koraiensis seedlings of understory layer and shurb layer.

• Journal of The Korean Association For Science Education
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• v.23 no.2
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• pp.117-130
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• 2003

The study is to analyze the middle school-science parts among the products of nation-wide science exhibition for eleven years recently and the results of compared to the content of science-textbooks of middle school. The number of observation in the student parts among the products of nation-wide science exhibition was the highest in all methods studied, whereas, most teachers surveyed experiments in their products. The mean Shannon's diversity index (DI) was same trend for students and teachers. There was a non-significant difference of the methods in students and teacher groups according to years and the association between students and teachers were closely connected with methods. The correlation between the content of students' products and texts of middle-school was highly positive for earth science, chemistry, and biology, whereas physics showed negative correlation.

• Journal of Ecology and Environment
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• v.36 no.3
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• pp.205-210
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• 2013

Cicuta virosa L. (Apiaceae) is a perennial emergent plant designated as an endangered species in South Korea. According to the former records, only four natural habitats remain in South Korea. A former study suggested that three of four populations (Pyeongchang: PC, Hoengseong: HS, Gunsan: GS) would be classified as different ecotypes based on their different morphological characteristics and life cycle under different environmental conditions. To evaluate this suggestion, we estimated genetic diversity in each population and distance among three populations by random amplification of polymorphic DNA. Seven random primers generated a total of 61 different banding positions, 36 (59%) of them were polymorphic. Nei's gene diversity and the Shannon diversity index increased in the order of PC < HS < GS, which is the same order of population size. In the two-dimensional (2D) plot of first two principal components in principal component analysis with the presence of 61 loci, individuals could be grouped as three populations easily (proportion of variance = 0.6125). Nei's genetic distance for the three populations showed the same tendency with the geographical distance within three populations. And it is also similar to the result of discriminant analysis with the morphological or life-cycle factors from the previous study. From the results, we concluded that three different populations of C. virosa should be classified as ecotypes based on not only morphology and phenology but genetic differences in terms of diversity and distance as well.

• Journal of Ecology and Environment
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• v.40 no.2
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• pp.84-88
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• 2016

To understand the effects of habitat characteristics on the genetic diversity of Persicaria thunbergii, three sites of different environmental conditions in a water system were surveyed. Site A was the closest to the source of the water system, and there was a dam between sites A and B. Site C is located on the lowest downstream in the water system. Vegetation survey of four quadrats at each site was performed, and soil samples were collected for physicochemical analysis. Random amplification of polymorphic DNA (RAPD) analysis of ten P. thunbergii individuals at each site was conducted to calculate population genetic diversity and genetic distance among populations. Soil was sterile sand at site A, whereas loamy soil at sites B and C. A pure stand of P. thunbergii appeared at site A, while other species occurred together (such as Humulus japonicus and Phragmites australis) at sites B (Shannon-Wiener index; $H_B=0.309$) and C ($H_C=0.299$). Similar to the species diversity, genetic diversity (Nei's gene diversity; h) within population of site A ($h_A=0.2381$) was relatively lower than sites B ($h_B=0.2761$) and C ($h_C=0.2618$). However, site C was separated from sites A and B in genetic distance rather than the geographical distance (Nei's genetic distance; A~B, 0.0338; B~C, 0.0685; A~C, 0.0833).