• 대한약침학회지
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• 제8권3호
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• pp.31-38
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• 2005

Objectives : This study was aimed at investigating liver protection mechanism of bear bile juice (Fel Ursi)by inducing liver toxicity through $CCl_4$ in mice and evaluated histological and serological findings. Methods : Experiment groups was categorized into untreated normal group, $CCl_4$ treated control group, and orally administered bear bile juice experiment group. At the termination of experiment, gross examination of the liver as well as histological findings, and Total protein, Albumin, Total bilirubin, Direct bilirubin SGOT, SGPT, and ALP contents in the serum were evaluated. Results : 1. For gross examination and histological findings, $CCI_4$ treated control group showed destroyed lobular structure, increased fibrosis, as well as hepatic cirrhosis. For the group treated with bear bile juice, the lobular structure suffered less damage, and showed lower level of fibrosis and liver cirrhosis compared to the control group. 2. For serum analysis, Total protein and Albumin were significantly increased in the bear bile juice experiment group than the control group. Total bilirubin and Direct bilirubin didn't show significant differences between the two groups. SOOT, SGPT, and ALP were significantly decreased in the normal and bear bile juice experiment groups compared to the control group. Conclusion : Taken together, bear bile juice can be effectively used for recovering the liver functions and further researches must be conducted to verify the efficacies of bear bile juice.

• 대한약침학회지
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• 제9권1호
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• pp.45-52
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• 2006

Objectives : This study was aimed at investigating liver protection mechanism of Moschus by inducing liver toxicity through $CCl_4$ in mice and evaluated histological and serological findings. Methods : Experiment groups was categorized into untreated normal group, $CCl_4$ treated control group, and orally administered Moschus experiment group. At the termination of experiment, gross examination of the liver as well as histological findings, and Total protein, Total bilirubin, Direct bilirubin SGOT, SGPT, and ALP contents in the serum were evaluated. Results : 1. For gross examination and histological findings, $CCl_4$ treated control group showed destroyed lobular structure, increased fibrosis, as well as hepatic cirrhosis. For the group treated with Moschus, the lobular structure suffered less damage, and showed lower level of fibrosis and liver cirrhosis compared to the control group. 2. For serum analysis, Total protein were significantly increased in the Moschus experiment group than the control group. 3. Total bilirubin didn't show significant differences between the two groups. but direct bilirubin was significantly increased in the Moschus experiment group than the control group. 4. SGOT, SGPT, were significantly decreased in the normal and Moschus experiment groups compared to the control group. 5. ALP was significantly decreased in the normal group compared to the control group, but Moschus experiment group didn't show significant differences compared to the control group. Conclusion : Taken together, Moschus can be effectively used for recovering the liver functions and further researches must be conducted to verify the efficacies of Moschus bile juice.

• Journal of Chest Surgery
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• 제22권4호
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• pp.562-572
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• 1989

The changes of serum glutamic oxaloacetic transaminase [SGOT], serum glutamic pyruvic transaminase [SGPT], serum lactic dehydrogenase [LDH] and serum creatine phosphokinase [CPK] were examined during and after the open heart surgery. In the total of 52 patients with heart diseases including 40 cases of congenital heart anomalies and 12 cases of acquired valvular heart diseases who undergone open heart surgery under cardiopulmonary bypass. The results obtained are as follows: 1. The average value of SGOT before surgery was 30.27 [ 18:86 units. The enzyme was reached to the maximum of 139.88 [ 89.43 units on the 1st day after the operation [p< 0.05], the enzyme activity was gradually decreased from the 3rd day after the operation, returned to the normal range on the 7th day after the operation. 2. The average value of SGPT before surgery was 14.36 [ 7.45 units. The enzyme was reached to the maximum of 34.67 [ 27.64 units on the 2nd day after the operation, but it was valueless statistically, the enzyme activity was gradually decreased from the 3rd day after the operation, returned to the normal range on the 5th day after the operation. 3. The average value of LDH before surgery was 263.07 * 86.66 units. The enzyme was reached to the maximum of 662.29 * 303.60 units on the 2nd day after the operation [p < 0.05], the enzyme activity was gradually decreased from the 5th day after the operation, returned to the normal range on the 7th day after the operation. 4. The average value of CPK before surgery was 141.35 * 43.44 units. The enzyme was reached to the maximum of 377.42 [ 222.02 units on the 1st day after the operation [p < 0.05], the enzyme activity was gradually decreased from the 5th day after the operation, returned to the normal range on the 7th day after the operation. 5. In the relationships of the serum enzymes and duration of the extracorporeal circulation, the values on the group over 90 minute of the extracorporeal circulation were increased than on the group below 90 minute of the extracorporeal circulation, but it was valueless statistically.

• Journal of Nutrition and Health
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• 제15권4호
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• pp.277-289
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• 1982

Because of a high degree of unsaturation of perilla seed oil vitamin E deficiency can be produced in chicks by using this particular oil diet. It is therefore convenient to use this oil for elucidating more detailed function (s) of vitamin E. The present study was undertaken to investigate the nutritional and pathological aspects both of high PUFA and of low vitamin E in the diet eventually to elucidate the function(s) of vitamin E. The present study examines the relationships between PUFA, vitamin E and Se in the experimental diets by measuring vitamin E levels both in serum and in liver and activities of SGPT and SGOT and by examining electron micrographs of the chick hepatocytes. Vitamin E concentrations in serum and liver responded to dietary treatments. Serum levels of vitamin E were more affected than those in liver, the values of groups IV(15% fat,- Vit.E) and V(15% fat,-Vit E,-Se) were significantly lower than those of groups I (5% fat) and 111 (15% fat) with P/S ratio of 1. The activities of SGOT and SGPT were found not to be different significantly among different diet groups. Electron microscopic observations of the chick hepatocyte revealed degeneration of mitochondria and appearance of vesicles in the cytoplasm of groups fed diets high in PUFA and deficient in vitamin E alone or deficient in both vitamin E and Se. In the same group pyknotic nuclei and deterioration of the sinusoidal border, showing diminution in microvilli were also observed. More detailed studies concerning biochemical aspects should be carried out by using radioisotopes both in the in vitro and in vivo systems and morphological study should have a focus on the changes in nucleus which seems already suggestive in the present observation.

• Journal of Preventive Medicine and Public Health
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• 제24권3호
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• pp.314-327
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• 1991

To evaluate the effects on health by exposure to mixed organic solvents, the author analyzed some labortory findings of blood (hemoglobin, hematocrit RBC, WBC, SGOT, SGPT) and urine (protein, blood, sugar) that were obtained from 722 workers (male:446, female:276) who worked in plywood, painting and shoes factories in Pusan area from January to December, 1990. The result were as follows ; 1. The mean values of each hematologic findings were all within normal limit but there were significant differences at hematocrit, RBC, WBC, SGOT in male and at WBC, SGPT in female at the comparison of the mean values and the distribution according to the normal criteria by type of work. 2. There were no differences in urinalysis(protein, blood, sugar) by type of work. 3. In stepwise multiple regression, analysis of hematologic findings on age, working duration and type of work, regression coefficients on age and type of work were relatively high in male and female sub1, respectively. As a result of this study, it is considered that the measurement of WBC with morphology is significant in hematologic findings and instead of spot urine and urinstix, 24 hours urine and quantitative analysis is required in urinalysis in screening test for healthy status of workers who deal with the organic solvents.

• Advances in Traditional Medicine
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• 제5권3호
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• pp.201-208
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• 2005

Earlier we have reported the antidiabetic activity of fresh juice of rhizomes of Zingiber officinale (Z. officinale) and its correlation with 5-HT receptor antagonism. Since 6-gingerol the marker compound of Z. officinale is reported to posses 5-HT anatgonistic activity, the present investigation, was undertaken to find out the concentration of 6-gingerol present in methanolic extract of Z. officinale and its different fractions (petroleum ether, toluene and chloroform). We also evaluated these fractions for antidiabetic activity in streptozotocin (STZ)-induced neonatal type 2 diabetic rats. Fasting glucose and insulin levels in non insulin dependent diabetes mellitus (NIDDM) rats were found to be significantly (P < 0.05) higher than control rats and these were significantly decreased by treatment with methanolic extract of Z. officinale and its fractions. The results of oral glucose tolerance test (OGTT) showed that methanolic extract and its fractions significantly (P < 0.05) decreased both STZ-induced increase in $AUC_{glucose}$ and $AUC_{insulin}$ values in NIDDM groups. Treatment with petroleum ether fraction produced a greater reduction in elevated glucose and $AUC_{glucose}$ levels as compared to treatment with other fractions. Treatment with methanolic extract of Z. officinale and its fractions also produced significant reduction in the elevated lipid, serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) levels in NIDDM rats. The effect of petroleum ether fraction on elevated lipid, SGOT and SGPT levels was significantly greater as compared to treatment with other fractions. The concentration of 6-gingerol was found to be maximum in petroleum ether fraction (11.430%) and minimum in chloroform fraction (0.973%). The methanolic extract and toluene fraction was found to contain 3.080% and 2.191 %, 6-gingerol respectively. In conclusion, our data suggest that methonolic extract and its fractions possess significant antidiabetic activity in NIDDM rats. The extent of activity appears to be dependent on the concentration of 6-gingerol present in the extract or its fractions.

• 한국식품영양과학회지
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• 제18권3호
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• pp.273-278
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• 1989

해동피 methanol 추출물을 시료로 mouse의 급성독성실험, 실험적으로 유발시킨 가토의 간장기능 장애에 미치는 영향을 실험하여 다음과 같은 결과를 얻었다. 실험결과 해동피 methanol 추출물의 급성독성 $(LD_{50})=7.3g/kg$으로 무독하였고 $CCI_4$로 손상시킨 가토의 간장기능회복에 있어서는 실험동물체중 kg당 시료 800mg투여군에서 14일째 대조군에 비해 SGPT가 $87.28{\pm}23.03$ unit로 서서히 유의성있게 회복되었다. 시료 800mg/kg투여군에서 alkaline phosphatase 활성도는 2일째부터, 그리고 total bilirubin 측정치는 6일째부터 현저히 회복되는 경향을 보였다. 특히 total cholesterol 측정치는 200mg/kg투여군에서 14일째 $99.36{\pm}12.42mg/d{\ell}$과 800mg/kg투여군에서 10일째 $96.69{\pm}18.40mg/d{\ell}$, 14일째 $80.68{\pm}8.77mg/d{\ell}$로 대조군에 비해 현저한 감소를 보였다.

• 한국식품과학회지
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• 제11권1호
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• pp.50-55
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• 1979

인삼 nectar, 인삼추출물이 함유된 orange juice, 인삼 drink 제등을 정상식이에 첨가 (555 mg Ginseng extract/kg of diet, 1,110 mg Ginseng extract/kg diet)하여 이유 직후의 Sprgue-Dawley strain 백쥐 40마리를 8마리씩 5군으로 나누어 12주간 사육하였다 12주후에 체중증가율, 사료효율, 장기의 무게, hematocrit value, serum cholesterol content, serum GOT and GPT 활성등을 측정하여 정상식이군과 비교한 결과 체중증가율, 사료효율, 장기의 무게, hematocrit value, SGOT 활성등을 모두 정상식이군에 비해서 실험군이 통계적으로 유의차를 보이지 않았으나, s-GOT 활성은 대조군에 비해 실험군이 다소 낮은 경향을 관찰할 수 있었고, s-cholesterol 농도는 대조군에 비해 다소 높은 경향이었으나 통계적인 유의차는 없었다. 백쥐 각군의 간, 신장, 비장등의 조직세포를 검사한 바 하등의 이상 조직을 발견하지 못하였다. 12주 실험이 끝난 후 체중을 측정하고 swimming test를 실시한 결과 대조군에 비해 실험군의 수영시간이 연장되었으며 V군에서는 유의성있게 연장되었다.

• Natural Product Sciences
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• 제14권2호
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• pp.95-99
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• 2008

In a project to study the hepatroprotective effect of some plant extracts four plants Ephedra foliate Boiss, Alhagi maurorum Medikus, Capsella bursa-pastoris (L.) Medik. and Hibiscus sabdariffa L. were studied. The ethanol extract of the aerial part of the first three plants and the flowers of H. sabdariffa were subjected to hepatoprotective assays using Wistar albino rats. Liver injury induced in rats using carbon tetrachloride. The biochemical parameters; serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP) and total bilirubin were estimated as reflection of the liver condition. Based on the good results of the biochemical parameters measurements, histopathological study was performed on the liver of rats treated with E. foliate. The normal appearance of hepatocytes indicated a good protection of the extract from carbon tetrachloride hepatotoxicity. All the results were compared with silymarin, the reference hepatoprotective drug.

• 대한수의학회지
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• 제13권2호
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• pp.131-136
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• 1973

Effect of Burdizzor's bloodless castration on blood picture, serum transaminase activity and growth of Korean calves were observed for 90 days period on indoor feeding. The results obtained were as follows; 1. In complete blood count, hemoglobin, hematocrit, total leucccyte count were shown only little changes comparing with that of controls. 2. In diffenentiaI count, the lymphocytes were significantly decreased in first week following castration, while the neutophils were relatively decreased (with a relative lymphocytosis) in later period of experiment (2 to 12 weeks). 3. Serum transaminase(SGOT and SGPT) and alkaline phosphatase activities were not influenced by Burdizzor's bloodless castration. 4. On average total gain of body weight, no significant differences were found between castrated and noncastrated groups during the experimental period.