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Effect of Skim Milk-Alginate Beads on Survival Rate of Bifidobacteria

  • Yu, Won-Kyu;Yim, Tae-Bin;Lee, Ki-Yong;Heo, Tae-Ryeon
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.6 no.2
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    • pp.133-138
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    • 2001
  • In this study, an attempt was made to increase the survival rate of bifidobacteria entrapped in alginate in the gastrointestinal tract, and to investigate the potential industrial applications, for example lyophilized capsules and yogurt. First, the protective effect of various food additives on bifidobacterial survivability was determined after exposure to simulated gastric juices and bile salts. The additives used in this study were skim milk (SM), polydextrose (PD), soy fiber (SF), yeast extract (YE), chitosan (CS), $\kappa$-carageenan ($\kappa$-C) and whey, which were added at 0.6% concentration (w/v) to 3% alginate-bifidobacterial solution. In the simulated gastric juices and bile salts, the protective effect of 0.6% skim milk-3% alginate (SM-A) beads on the survival rate of bifidobacteria proved to be higher than the other additives. Second, the hydrogen ion permeation was detected through SM-A vessel without bifidobacterial cells at different SM concentrations (0.2%, 0.4%, 0.6%, 0.8%, and 1.0%). There were no differences in terms of the pH decrease in SM-A vessels at 0.6%, 0.8%, and 1.0% (w/v) SM concentrations. The survival rate of bifidobacteria in SM-A beads would appear to be related to the SM buffering capacity against hydrogen ions and its tendency to reduce the pore size of bead. In this experiment, the survival rate of bifidobacteria entrapped in beads containing 0.6% SM showed the highest viability after exposure to simulated gastric juices for 3h, thereby indicating that 0.6% SM is the optimum concentration fir 3% alginate bead preparation. Third, the effect of SM-A beads on the freeze-drying and yogurt storage for 10 days was investigated. SM-A beads were found to be more efficient for freeze drying and yogurt storage than untrapped cells and the alginate bead. Consequently, the survival rate of bifidobacteria entrapped in SM-A beads was increased in simulated gastric juices, bile salts and probiotic products, such as lyophilized capsules and yogurt, SM-A beads can be expected to produce high value probiotic products.

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Job Stress Associated with Problematic Alcohol Drinking in Korean Employees (한국 직장인의 문제성 음주와 관련된 직무 스트레스 요인)

  • Lee, Daeun;Lim, Se-Won;Shin, Dong-Won;Oh, Kang-Seob;Shin, Young-Chul
    • Anxiety and mood
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    • v.13 no.1
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    • pp.39-45
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    • 2017
  • Objective : Korean employees tend to release their stress by alcohol drinking. This study aimed to investigate the association between job stress and problematic drinking in Korean employees. Methods : The study participants were 72,119 employees who attended health screenings and responded to the Alcohol Use Disorder Identification Test-Korea (AUDIT-K), Korean Occupational Stress Scale-Short Form (KOSS-SF), Center for Epidemiologic Study-Depression (CES-D), Beck Anxiety Inventory (BAI) and questionnaire on suicidal ideation and sociodemographic characteristics. We investigated the sociodemographic and clinical characteristics of the problematic drinking group ($AUIDT-K{\geq}12$) and used binary logistic regression to determine the impact of job stress on problematic drinking. Results : Age, gender, marital status, education year, sleep duration, obesity, smoking, suicidal ideation, and depression and anxiety showed significant differences between the problematic drinking group and normal group. Among the various kinds of job stresses, job insecurity and lack of reward were associated with problematic drinking. Conclusion : The result of this study suggested that certain kinds of job stress might be associated with problematic drinking in Korean employees.

A Study on the Costumeplay Fashion(2) (코스튬플레이 패션에 대한 연구(2))

  • Lee, Eui-Jung;Baik, Cheon-Eui
    • Journal of the Korea Fashion and Costume Design Association
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    • v.9 no.3
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    • pp.181-189
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    • 2007
  • This study aims to conduct research into the property of costumeplay that has been established with our unique characteristics despite the critics from the existing generation, and the cospre style preferred by costumeplayers. The research methods include literature study, existing research, and a survey targeting costumeplayers who make costumes upon order or borrow costumes on websites. The findings are as follows. 1. From the results of the survey, 77% of the participants in the costumeplay were secondary school students, 16% were college students, and 5% were primary school students. 2. The distribution of the residences of the costumeplayers was found to be mostly centered in two areas including Seoul/Gyeonggi and Gyeongsang/Busan, which were 39% and 37% each. This is probably because Seoul Comic and Busan Comic were regularly taking place, and these are relatively economically affluent areas and locations where the rapid exchange of Japanese culture occurs. 3. For the cospre style preferred by costumeplayers, 57% of respondents preferred costumes for reviving characters, 18% of them for fan-cospre and 14% of them for reviving a character and creating new characters. Costumeplayers preferred cospre costumes for reviving characters rather than for creating new characters. 4. Most respondents preferred a character-oriented design, posting 31.9%. Next, 17.5% of them selected the Lolita look This is probably because the Lolita look which is much favored in the latest fashion trends affects them, and the rest of the respondents preferred a fantasy/SF group, visual, uniform, and maid look, in order. 5. For costs required for costumeplay for 1 time, 39% of respondents spent about KRW $100,000{\sim}150,000$, and 24% of them about KRW $150,000{\sim}200,000$. However, for buying accessories or small items, over 50% of them spent KRW $30,000{\sim}40,000$.

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Effects of a Water Exercise on the Lower Extremities Coordination during Obstacle Gait in the Female Elderly - Focusing on Training and Detraining Effects - (수중운동이 여성노인 장애물보행 시 하지 협응에 미치는 영향 - 훈련 및 훈련잔여효과 중심으로 -)

  • Yoon, Sukhoon;Chang, Jae-Kwan;Kim, Joonyeon
    • Korean Journal of Applied Biomechanics
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    • v.24 no.2
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    • pp.95-101
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    • 2014
  • The purpose of this study was to investigate the training and detraining effects of a 8-week water exercise on lower extremities coordination during obstacle gait in the female elderly. Eight elderly participants (age: $76.58{\pm}4.97$ yrs, height: $148.88{\pm}7.19$ cm, body mass: $56.62{\pm}6.82$ kg, and leg length: $82.36{\pm}2.98$ cm), who stayed at the Seoul K welfare center, were recruited for this study. All participants had no history of orthopedic abnormality within the past 1 year and completed the aquatic exercise program which lasted for 8 weeks. To identify the training and detraining effect of 8 weeks of water exercise, a 3-D motion analysis with 7 infrared cameras and one force plate sampling frequency set at 100 Hz and 1,000 Hz, respectively, was performed. A two-way ANOVA was performed to find training and detraining effects among diferent obstacle heights. In this study significant level was set at .05. Significant training effects of LTS (lead foot thigh and shank) coordination in all obstacle height were found (p<.05). It is also found that the training effect of LTS remained 37%, 58%, and 25% in obstacle height of 30%, 40%, and 50%, respectively. Lead foot showed the greater detraining effect of coordination compared with trail foot, and SF (shank and foot) coordination revealed better detraining effects of coordination compare with TS (thigh and shank) in both feet. Based on the findings, a 8 week water exercise give an positive effects to the elderly in terms of segment cooperation which potentially helps reducing their accident falls. The magnitude of detraining may also help the elderly to find the retraining moment.

Mix design and early-age mechanical properties of ultra-high performance concrete

  • Tang, Chao-Wei
    • Advances in concrete construction
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    • v.11 no.4
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    • pp.335-345
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    • 2021
  • It is known from the literature that there are relatively few studies on the engineering properties of ultra-high performance concrete (UHPC) in early age. In fact, in order to ensure the safety of UHPC during construction and sufficient durability and long-term performance, it is necessary to explore the early behavior of UHPC. The test parameters (test control factors) investigated included the percentage of cement replaced by silica fume (SF), the percentage of cement replaced by ultra-fine silica powder (SFP), the amount of steel fiber (volume percent), and the amount of polypropylene fiber (volume percentage). The engineering properties of UHPC in the fresh mixing stage and at the age of 7 days were investigated. These properties include freshly mixed properties (slump, slump flow, and unit weight) and hardened mechanical properties (compressive strength, elastic modulus, flexural strength, and splitting tensile strength). Moreover, the effects of the experimental factors on the performance of the tested UHPC were evaluated by range analysis and variance analysis. The experiment results showed that the compressive strength of the C8 mix at the age of 7 days was highest of 111.5 MPa, and the compressive strength of the C1 mix at the age of 28 days was the highest of 128.1 MPa. In addition, the 28-day compressive strength in each experimental group increased by 13%-34% compared to the 7-day compressive strength. In terms of hardened mechanical properties, the performance of each experimental group was superior to that of the control group (without fiber and without additional binder materials), with considerable improvement, and the experimental group did not produce explosive or brittle damage after the test. Further, the flexural test process found that all test specimens exhibited deflection-hardening behavior, resulting in continued to increase carrying capacity after the first crack.

Assessment of flowing ability of self-compacting mortars containing recycled glass powder

  • Alipour, Pedram;Namnevis, Maryam;Tahmouresi, Behzad;Mohseni, Ehsan;Tang, Waiching
    • Advances in concrete construction
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    • v.8 no.1
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    • pp.65-76
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    • 2019
  • This paper investigates the effect of recycled glass powder (RGP) on flowing properties of self-compacting mortars (SCMs) containing different ratios of fillers and superplasticizer dosages. Fly ash (FA), nano-silica (NS), micro-silica (MS), metakaolin (MK) and rice husk ash (RHA) are used as fillers and their synergistic effect with RFP is studied. The effects of fillers and high-range water reducer (HRWR) on flowing ability of mortars are primarily determined by slump flow and V-funnel flow time tests. The results showed that for composites with a higher RGP content, the mortar flowing ability increased but tended to decrease when the composites containing 10% MK or 5% RHA. However, the flowing ability of samples incorporating 5% RGP and 10% SF or 25% FA showed an opposite result that their slump flow spread decreased and then increased with increasing RGP content. For specimens with 3% NS, the influence of RGP content on flowing properties was not significant. Except RHA and MS, the fillers studied in this paper could reduce the dosage of HRWR required for achieving the same followability. Also, the mixture parameters were determined and indicated that the flowability of mixtures was also affected by the content of sand and specific surface area of cement materials. It is believed that excess fine particles provided ball-bearing effect, which could facilitate the movement of coarse particles and alleviate the interlocking action among particles. Also, it can be concluded that using fillers in conjunction with RGP as cementitious materials can reduce the material costs of SCM significantly.

Effect of Cordyceps militaris with probiotics supplement on growth performance, meat quality characteristics, storage characteristics and cordycepin content of the breast meat in broilers

  • An, Jae Woo;Lee, Ji Hwan;Oh, Han Jin;Kim, Yong Ju;Chang, Se Yeon;Go, Young Bin;Song, Dong Cheol;Cho, Hyun Ah;Cho, Jin Ho
    • Korean Journal of Agricultural Science
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    • v.48 no.3
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    • pp.423-432
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    • 2021
  • This experiment was conducted to investigate the effect of Cordyceps militaris with probiotics (CMP) supplementation on the growth performance, meat quality and storage characteristics, and cordycepin content in the meat. Sixty one-day-old broilers (Ross 308) were allotted to two treatment groups of 30 each. In addition, six broilers were randomly assigned to a cage in the two treatment groups. The two dietary treatments were as follows: Control (CON) and basal diet + 0.5% of CMP. Body weight and feed intake were measured on the 1st, 14th, and 28th days from the start of the experiment. On days 1 - 14, the supplementation of CMP improved (p < 0.05) the body weight gain (BWG) and feed conversion ratio (FCR). Additionally, the feed intake (FI) and FCR scores in the CMP groups improved (p < 0.01) compared to the CON during the entire period. For the meat quality characteristics, water holding capacity (WHC), cooking loss (CL), redness (a*) in meat color value, and shearing force (SF) for the CMP group were improved (p < 0.01) compared to the CON group. For the meat storage characteristics, pH and thiobarbituric acid reactive substances (TBARS) were improved (p < 0.01) when the broilers were fed CMP compared to the CON group. Broilers fed CMP had a higher (p < 0.01) cordycepin content in the meat compared to the CON group. In conclusion, CMP improves the growth performance and meat quality of broilers.

Genetic identification of Sinomenium acutum based on chloroplast gene ndhF sequences

  • Ryuk, Jin Ah;Lee, Hye Won;Ko, Byoung Seob
    • The Korea Journal of Herbology
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    • v.28 no.5
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    • pp.1-6
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    • 2013
  • Objectives : This study was conducted to identify the original Sinomini Caulis et Rhizoma plant among Stephania tetrandra, Cocculus trilobus, and Aristolochiae fangchi to develop the genetic marker for Sinomini Caulis et Rhizoma. Methods : Sinomenium acutum was identified by the classification and identification committee of the National Center for Standardization of Herbal Medicines. The chloroplast ndhF gene was amplified. We performed sequences alignment analysis of Sinomenium acutum, Stephania tetrandra, C. trilobus, and A. fangchi using BioEdit program. The SFR markers designed were consisted of SF01, SR04, and SR05 primers. Results : Many variations of Sinomeni Caulis et Rhizoma are currently commercialized as herbal medicine. We compared the base sequences of the ndhF intergenic space of chloroplast DNA with Sinomenium acutum, Stephania tetrandra, C. trilobus, and A. fangchi. According to the results, it showed that the nucleotide variations were seen in 30 genes of four species. Phylogenetic analysis revealed that 4 species were classified into five groups based on an inter-group divergence in nucleotide sequence of 9%. We developed SFR marker nucleotides enough to authenticate respective species and confirmed its application on the band size at 419 base pair. These sequence differences at corresponding positions were available genetic markers to identity the Sinomeni Caulis et Rhizoma. Conclusions : Base on these results, the ndhF region was effective in distinguishing Sinomini Caulis et Rhizoma The SFR genetic marker was useful for identifying Sinomini Caulis et Rhizoma with other species.

The Influence of COVID-19 on Physical Activity, Sleep Quality, and the Quality of Life in Adults by Age Group

  • Kim, Tae-Yeon;Choi, Sil-Ah
    • The Journal of Korean Physical Therapy
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    • v.34 no.1
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    • pp.18-25
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    • 2022
  • Purpose: The purpose of this study was to identify changes in physical activity, sleep quality, and quality of life (QOL) during COVID-19 in adults by age group. Methods: An online survey was conducted on a total of 160 participants who were divided into three groups by age; young adults, middle-aged adults, and old adults. The participants responded to the self-reported assessment of the impact of social distancing during COVID-19, International Physical Activity Questionnaire (IPAQ), Pittsburgh Sleep Quality Index (PSQI), and Short Form-36 Health Survey (SF36). Descriptive statistics, analysis of variance, and Pearson's correlation were used for data analysis. Results: There were significant differences between the three age groups in physical activity (p<0.05) and PSQI (p<0.01) and a post hoc analysis showed that the young adults' group had significantly higher physical activity than the old adults' group, while the old adults' group had significantly lower scores of PSQI (better sleep quality) than the others. The young adults' group showed a significant negative correlation between the stress from social distancing and QOL (r=-0.27, p<0.05) and between PSQI and QOL (r=-0.48, p<0.05). For the middle-aged adults' group, there was a significant negative correlation between PSQI and QOL (r=-0.53, p<0.05). The old adults' group showed a significant negative correlation between the stress from social distancing and physical activity (r=-0.35, p<0.05) and PSQI (r=-0.50, p<0.05), while there was a significant positive correlation between physical activity and PSQI (r=0.30, p<0.05) and QOL (r=0.30, p<0.05). Conclusion: The results of this study could be used as basic data for the promotion of physical and mental health in the post-COVID-19 era.

New phenomenological creep model for predicting creep of concrete with silica fume

  • Zgheib, Elise;Sawma, Rodolph;El Khoury, Judith;Raphael, Wassim
    • Advances in concrete construction
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    • v.14 no.1
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    • pp.71-77
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    • 2022
  • Creep phenomenon affects the stability and integrity of concrete structures. An inaccurate prediction of these strains may lead to the appearance of cracks and excessive deflections which may cause in some cases the demolition of structures. In fact, the measured values of these uncontrolled strains appear often to be clearly different and larger than the expected ones. Therefore, an accurate prediction of concrete deformations is a necessity. As a matter of fact, the codified descriptions of this phenomenon are unreliable and don't consider the effect of admixtures. The physical nature of creep is not well understood and almost all creep models are mainly of empirical nature. To overcome this issue, a study of the correlation between different parameters affecting concrete creep is performed and a new model for predicting creep of concrete is elaborated. This new model considers the effect of admixtures, specifically the silica fume, in predicting concrete creep and allows an accurate prediction of this phenomenon. The proposed model is based on the observation of physical behavior of creep phenomenon. It targets at expressing creep compliance in terms of structural and environmental parameters. In fact, the experimental observations show that creep curves follow two kinetic regimes leading to a model called Phenomenological Creep Model. By adequate regressions and substitutions, and according to this model, we can express creep compliance in terms of structural, environmental parameters and admixture types and percentage. The proposed new Phenomenological Creep Model Silica Fume (PCM19SF) calculates accurately creep of concrete by considering the effect of silica fume.