• Title/Summary/Keyword: SEED GERMINATION

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Effects of Aeration Period and Temperature after Imbibition on Growth of Mungbean Sprouts (침종 이후의 Aeration 기간과 온도에 따른 숙주나물의 생장)

  • Kang Jin Ho;Ryu Yeong Seop;Yoon Soo Young;Jeon Seung Ho;Cho Sook Hyon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.6
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    • pp.472-476
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    • 2004
  • Lateral roots formed on mungbean sprouts should lower their quality. The study was carried out to clarify the effects of aeration periods (AP; 1, 2, 3, and 4 hours) and temperature (AT; 20, 30, and $40^{\circ}C$) after 5 hour seed imbibition into 50 ppm benzyladenopurine (BA) solution on growth and morphological characters of mungbean (cv. Keumseongnogdu, Owoolnogdu and Zhong Lu 1) sprouts. On the 6th day, the mungbean sprouts were sorted by 4 categories on the base of hypocotyl lengths; > 7cm, 4 to 7cm, < 4cm, and non-germination to calculate their composition rates, number of lateral roots, lengths of hypocotyl and root, diameters at middle and upper parts of hypocotyl, fraction fresh and dry weights were measured. AP more affected growth of the cultivars than AT showing little effect on them. In the composition rate of the above 4 categories, cv. Zhong Lu 1 had the highest rate in longer than 7cm but nearly the same rate in AP treatments. Rates of longer than 4cm hypocotyls in cv. Keumseongnogdu, Owoolnogdu were increased with longer AP but their rates of shorter than 4cm showed reverse response to the former. Formation rate and number of lateral roots per sprout were decreased with longer AP, showing more severe decrement when delayed 3 to 4 hour AP. Upper part of hypocotyls and roots were more thickened and shortened in longer AP, respectively. Total fresh weights had no significant difference between AP treatments while hypocotyl fresh weights were increased with longer AP. It was concluded that in mungbean sprout culture aeration from BA treatment to the first watering permitted at least 4 hours.

Identification of Biologically Active Substances from Medicinal Plants (약용식물(藥用植物)(음나무, 오가피)로부터 생리활성물질(生理活性物質) 검정(檢定))

  • Lee, I.J.;Kim, K.U.
    • Korean Journal of Weed Science
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    • v.7 no.3
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    • pp.289-298
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    • 1987
  • This study was conducted to investigate the presence of biologically active substances such as phenolic acids, fatty acids and organic acids in the medical plants like Kalopanax pictum and Acanthopanacis cortex. Alcohol extracts of K. pictum and A. cortex showed complete inhibition of lettuce seed germination, indicating that these plants contained the biologically active substances. Eleven phenolic acids including protocatechuic acid were identified from K. pictum and A. cortex by GLC, and the contents of total phenolic acid were 1.7917mg/g in K. pictum, and 0.9567mg/g in A. cortex. Polyphenols such as neochlorogenic acid, chi orogenic acid, scopoletin, rutin and kaempferolglycoside which were not detected by GLC were anayzed by HPLC, and among phenolic acids indentified chi orogenic acid seemed to be the major acid in both K. pictum and A. cortex presented in amount of 23.7 and 13.0ppm, respectively. K. pictum contained 5.26mg/g of fatty acids and 27.69mg/g of organic acids, and A. cortex possessed 3.22mg/g of fatty acids and 9.80mg/g of organic acids, linoleic and oxalic acid appeared to be the major fatty and organic acids, representing more than 50% of total fatty acids and 80% of total organic acids.

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Identification of Physiologically Active Compounds from Purslane(Portulaca oleracea L.) (쇠비름(Portulaca oleracea L.)에 함유(含有)된 생리활성물질(生理活性物質) 탐색(探索))

  • Park, J.S.;Kim, K.U.
    • Korean Journal of Weed Science
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    • v.8 no.2
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    • pp.169-175
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    • 1988
  • This experiment was conducted to determine the presence of allelopathic substance in Portulaca oleracea L. Water and methanol extract from P. oleracea markedly inhibited the germination of lettuce, rice, raddish etc., indicating the presence of biological substances. The biochemical substances such as ferulic, p-coumaric, salicylic, vanillic, p-hydroxybenzoic acid etc., belonging to phenolic compounds were detected in a large amount, which may be responsible for exhibiting inhibitory effects. Various phenolic compounds were detected from different samples such as freshly, dried plants, seeds and callus. The highest amount of tannic and gallic acids were detected in dried samples of P. oleracea, the highest grantity of salicylic and vanillic acids in fresh samples, the largest amount of ferulic acid in seed sample, the highest amount of p-hydroxybenzoic and p-chlorobenzoic acids in callus. Linolenic acid was presented in amount of 9.203 mg/g in dried plant of P. oleracea as one of the major fatty acids and oxalic acid presented 27.941 mg/g as one of the major organic acids. These compounds seemed to be related to inhibitory effect of P. oleracea which needs further study.

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A New Cymbidium Cultivar 'Orange Bowl' with Orange Colored Flower and Medium Sized Plant (오렌지색계 중형 심비디움 'Orange Bowl' 육성)

  • Kim, Mi-Seon;Rhee, Hye-Kyung;Park, Sang-Gun;Jung, Hyang-Young;Choi, Sung-Yul;Lim, Jin-Hee
    • Horticultural Science & Technology
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    • v.29 no.6
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    • pp.651-654
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    • 2011
  • Cymbidium 'Orange Bowl' (Lucky Rainbow 'Randevous' ${\times}$ 'Eastern Star') was developed from a cross between hybrids at the National Institute of Horticultural & Herbal Science, Rural Development Administration in 2006. A cross was made between the pink colored flower C. Lucky Rainbow 'Randevous' as maternal line and pure yellow colored flower, C. 'Eastern Star' as paternal line in 1995. The seed germination, cultivation, selection, and characteristic trials were conducted from 1996 to 2006. The line was named as Wongyo F1-18 and phenotype was characterized in 2006 as a new 'Orange Bowl'. The 'Orange Bowl' has having light yellow basal color (RHS, YO21D) and orange line (RHS, OR30B) on both of sepal and petal with red lip (RHS, OR30B). 'Orange Bowl' has about 10.9 flowers per flower stalk and flower size of 7.4 cm. General appearance of the petals and sepals is slightly incurved shape. The plant size is intermediate having erect peduncle. Blooming is started from the late of January (mid winter) under optimal culture condition. Leaf attitude and twisting is half- erect and very weak respectively. This hybrid has attractive floral arrangement, long flower stalk (71.8 cm) and vigorous growth. We expect that 'Orange bowl' has a great potential for exporting to Chinese market.

Synthesis of Anilide Derivatives from Amino Acids and their Biological Activities -Part II. Synthesis of N-Benzoylglycylanilide and N-Benzoylalanyanilide Derivatives and their Biological Activities- (Amino산(酸)으로부터 Anilide유도체(誘導體)의 합성(合成)과 생리활성(生理活性)에 관(關)하여 -제2보(第二報). N-Benzoylglycylanilide 및 N-Benzoylalanylanilide 유도체(誘導體)의 합성(合成)과 생리활성(生理活性)에 관(關)한 연구(硏究)-)

  • Kim, Jung-Bea;Lee, Chun-Soo
    • Applied Biological Chemistry
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    • v.26 no.4
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    • pp.231-238
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    • 1983
  • N-Benzoylglycylanilide and N-benzoylalanylanilide derivatives were synthesized from N-benzoylglycine and N-benzoylalane with anilines. respectively. The products were identified by elemental analysis, IR, UV and NMR spectra with N-benzoylglycylanilide (I), N-benzoylglycyl-o-toluidide(II), N-benzoylglycyl-m-toluidide(III), N-benzoylglycyl-p-toluidide(IV), N-benzoyl-L-alanylanilide(V), N-bnzoyl-L-alanyl-o-toluidide(VI), N-benzoyl-L-alnnyl-m-toluidise(VII), N-benzoyl-L-alanyl-p-toluidide(VIII). These compounds were tested for their phytotoxicity to the germination and seedling growth of rice, radish, green pea plants and turf grass. Among them, N-benzoyl-Lalanyl-m-toluidide had strongly inhibitory effect on the seedling growth of radish seeds, and N-benzoyl-L-alanylanilide showed an inhibitory activity especially upon the seedling growth of turf seeds. In addition, the inhibitory rate of plant seed growth differed with the isomeric position(ortho, meta and para) of methyl group; N-benzoylglycyl-m-toluidide was more effective than both N-benzoylglycyl-o-toluidide and N-benzoylglycyl-p-toluidide derivatives, and also N-benzoyl-L-alanyl-m-toluidide was more effective than both N-benzoyl-L-alanyl-o-toluidide and N-benzoyl-L-alanyl-p-toluidide.

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Physicochemical Properties and Growth Characteristics of Wood Chip and Peat Moss Based Vegetation Media (우드칩과 피트모스를 원료로 하는 식생기반재의 물리·화학적 특성 및 생육 특성)

  • Kim, Ji-Su;Jung, Ji Young;Ha, Si Young;Yang, Jae-Kyung
    • Journal of the Korean Wood Science and Technology
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    • v.44 no.3
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    • pp.323-336
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    • 2016
  • This study was carried out to evaluate the physicochemical properties of steam exploded wood chips for peat moss substitute in vegetation media. Also, the mixtures at the different ratios of peat moss and pretreated wood chips (90 : 10, 70 : 30 and 50 : 50 (w/w), respectively) were evaluated by physicochemical and plant growth characteristics. The pretreated wood chips was showed that bulk density, porosity and pH were $0.26g/cm^3$, 93.3% and 5.7, respectively. This result indicates that physicochemical properties was improved when wood chips was apply to steam explosion in the range of optimum physicochemical condition for vegetation media. In particular, the mixture ratio of peat moss and pretreated wood chips to 70 : 30 (w/w) showed higher seed germination, plant height and leaf growth than peat moss. Also, the bulk density, porosity, water holding capacity, pH and C/N ratio were $0.20g/cm^3$, 91.8%, 76.1%, 5.2 and 51.0 in the range of optimum physicochemical condition for vegetation media.

Evaluation of Insecticidal Activity of Plant Extracts against the Diamondback Moth, Plutella xylostella (Lepidoptera: Plutellidae) on Vegetable Plant (배추좀나방(Plutella xylostella)에 대한 식물추출물의 살충력 평가)

  • Choi, I-Jin;Kwon, Hyuk-Hyun;Lee, Han-Ho;Son, Hyoung-Gi;Hong, Sang-Kil;Kang, Jong-Woon;Park, Yu-Soon
    • Korean Journal of Plant Resources
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    • v.26 no.1
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    • pp.19-25
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    • 2013
  • This study focused on the usefulness of plant extracts for a city organic farm. Insecticidal activity of 8 plant species, plants crude extracts by ethanol and water, against third instar larvae (susceptible strain) of Plutella xylostella was evaluated in this study. Ethanol(25%) extracts of plant materials had better insecticidal activity than water extracts at the room temperature. Insecticidal activity were investigated by leaf disc spray method. Five plant extracts (Capsicum annuum, Chelidoniun majus, Leonurus sibiricus, Coptis japonica, Mentha piperascens) showed over 50% insecticidal activity to P. xylostella at the concentration of $1500mg{\cdot}L^{-1}$. Extract of C. annuum showed the highest insecticidal activity (83.3%) against P. xylostella. Phytotoxicity was not observed on Raphanus sativus and Brassica campestris with spray application. Seed germination and growth of two plants were not affected. Extract of C. japonica revealed 73.3% control efficacy against P. xylostella of R. sativus and showed 70% control efficacy against those of B. campestris, respectively, in plastic house. Insecticidal activity against various insects was observed in C. japonica extract as 87.4% for R. sativus and 74.3% for B. campestris in the field. These results suggested that extracts of C. annuum, C. japonica showed the highest insecticidal activity, and could be used as city farm insecticides for organic farming.

Effects of Cultural Condition on Seed Germination and Seedling Growth of Elsholtzia angustifolia Kitag. (가는잎향유(Elsholtzia angustifolia Kitag.)의 종자발아 및 유묘생육에 미치는 재배조건의 영향)

  • Jeong, Eun-ah;Jeon, Ki-beom;Choi, Hye-min;Moon, Sang-a;Yeon, Su Ho;Lee, Seong Yeon;Kwon, Soon-tae;Lee, Cheol Hee
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.10a
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    • pp.56-56
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    • 2018
  • 가는잎향유는 우리나라에 자생하는 고유종으로 꽃이 아름답고 향이 좋아 관상 및 약용으로 사용되나, 무분별하게 남획되기도 하여 자원의 보호가 요구되는 실정이다. 이에 본 연구는 가는 잎향유의 종 유지 및 재배방법 확립을 위하여 종자를 이용한 육묘조건을 조사하였다. 연구 내용은 파종용기, 파종립수, 토양종류, 추비농도 및 차광정도 등을 각 처리별로 2017년 6월 2일부터 7월 31일까지 약 8주간 수행되었다. 가는잎향유의 종자를 162, 200, 288구 트레이 및 사각분에 파종한 처리 중, 162구 트레이 처리구에서 엽면적과 지하부 생체중이 가장 넓거나 무거웠으며, 파종립수는 한 cell당 2립 처리구에서 초장이 안정적이고 엽면적이 넓었다. 파종용 상토로는 피트모스:펄라이트(3:1) 처리구가 코코피트:펄라이트(3:1) 처리구보다 생육에 효과적이었으며, 비료는 혼용토에 $300-200-200mg{\cdot}L^{-1}$(N-P-K)를 혼합한 처리구에서 발아율, 마디수, 엽록소 지수 등이 우수하였다. 재배관리는 추비는 hyponex를 농도별로 시비하였으나, 무처리구보다 생육에 효과적이지는 않았다. 또한, 재배시 차광처리를 하지 않는 것이 가는잎향유의 실생육묘에 유리하였다. 따라서, 가는잎향유의 육묘를 위해서는 피트모스:펄라이트(3:1) 혼용토에 비료를 $300-200-200mg{\cdot}L^{-1}$를 첨가한 토양을 162구 트레이에 충진하여, 한 cell에 2립씩 파종하고 무차광 조건에서 재배하며, 추비는 처리하지 않는 것이 생육에 가장 효과적일 것으로 생각된다.

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Studies on Proper Medium for Somatic Embryogenesis in Suspension Culture of Rehmania glutinosa and Encapsulation of Somatic Embryos (지황의 현탁배양에서 체세포배 형성에 관여하는 요인분석과 체세포배의 Encapsulation)

  • Park, Ju-Hyun;Park, Sang-Un;Chae, Young-Am
    • Korean Journal of Medicinal Crop Science
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    • v.3 no.2
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    • pp.100-106
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    • 1995
  • This study was conducted to find the factors affecting somatic embryogenesis in suspension culture of Rehmania glutinosa and investigate the possibility of artificial seed production by encapsulation of somatic embryos. Linsmeier-Skoog medium was appeared as proper for somatic embryogenesis. Sucrose with $3{\sim}5%$ as carbon sources was good for somatic embryogenesis, and both ammonium and nitrate nitrogen were necesary for normal somatic embryo production. BA with NAA or kinetin with NAA were better than the use of cytokinin alone for both somatic embryogenesis and numbers of somatic embryos. $AgNO_3$ as protectant for vitrification of seedlings in vitro culture had no harmful effect on somatic embryos. Sphericity of encapsulated seeds was good at 3% gel of sodium alginate but germination was better at 2.5% sodium alginate level. Artificial seeds were germinated and developed normal shoots and roots under in vitro condition.

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The Ecological Characteristics by Micro-Topographies of Beech forest in Ulleung Island (울릉도 너도밤나무림의 미세지형별 생태적 특성)

  • Han, Sang-Hak;Yun, Chung-Weon;Song, Ju-Hyeon;Kim, Ho-Jin;Lee, Jeong-Eun;Yun, I-Seul;Yoo, Yoon-Seo;Lee, Sang-Hun
    • Korean Journal of Environment and Ecology
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    • v.33 no.6
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    • pp.686-694
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    • 2019
  • This study was carried out to identify the long-term changes to beech forest due to climate change in Ulleung-do. To study the traits of the stand structure of beech forest with micro-topography, we investigated the distribution of the basal area, important values, and indicated species in a small ridge between Albong basin and Seongin-bong peak, and 1-ha stands (20 m × 20 m, 25 sites) dominated beech species including on the upper slope and valley parts. The result showed that the indicator species characterizing the vegetation communities were Tsuga sieboldii, Rhododendron brachycarpum, and Mitchella undulata in the small ridge and Celtis jessoensis, Dryopteris crassirhizoma, and Ulmus laciniata in the valley part. Moreover, the individuals with the total DBH < 10 cm were more predominant in the small ridge and upper slope than in the valley part, the individuals with DBH = 10-25 cm were predominant in the small slope, and individuals with DBH = 25-45 cm and DBH > 45 cm were predominant in the upper slope. The reason for the predominant distribution of beech stand with DBH<10 cm in all terrains is suggested to be germination by nutritional propagation rather than secondary succession caused by artificial disturbances and seed propagation.