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Genetic Variability Based on Randomly Amplified Polymorphic DNA in Mistletoe Fig (Ficus deltoidea Jack) Collected from Peninsular Malaysia

  • Bhore, Subhash Janardhan;Arneida H., Nurul;Shah, Farida Habib
    • Journal of Forest and Environmental Science
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    • v.25 no.1
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    • pp.57-65
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    • 2009
  • Ficus deltoidea Jack is an important and popular medicinal plant species found in the Malaysia. Plants are being collected and used based on morphology and authentication to prevent adulteration is not in practice. In this study, twenty-six accessions of F. deltoidea Jack were collected from Kelantan and Terengganu states of Peninsular Malaysia to examine their genetic similarities and differences using randomly amplified polymorphic DNA (RAPD) technique. Out of 20 arbitrary primers, two primers (D-10 and D-11) were selected which produced reliable DNA polymorphism. D-10 and D-11 primers generated 138 RAPD bands ranging from 250 bp to 3000 bp. Ninety-nine of them were polymorphic loci (72%) and thirty-nine were nonpolymorphic loci (28%). A total of 56 bands with polymorphic loci were amplified with primer D-10 and analyzed by cluster analysis and UPGMA to present a dendrogram depicting the degree of genetic relationship among 26 accessions. Eight RAPD markers were sequenced to determine their identity. RAPD analysis showed the genetic diversity among 26 accessions of F. deltoidea Jack. The RAPD profile and RAPD marker sequences reported in this paper could be used in plant and/or plant material authentication. This study also suggested that RAPD can be a useful technique to study DNA polymorphism in F. deltoidea Jack.

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Investigation on the Dielectric, Physical and Chemical Properties of Palm Oil and Coconut Oil under Open Thermal Ageing Condition

  • Mohamad, Nur Aqilah;Azis, Norhafiz;Jasni, Jasronita;Kadir, Mohd Zainal Abidin Ab;Yunus, Robiah;Ishak, Mohd Taufiq;Yaakub, Zaini
    • Journal of Electrical Engineering and Technology
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    • v.11 no.3
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    • pp.690-698
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    • 2016
  • In this paper, a study is carried out to investigate the dielectric, physical and chemical properties of Palm Oil (PO) and Coconut Oil (CO) under open thermal ageing condition. The type of PO used in this study is Refined Bleached and Deodorized Palm Oil (RBDPO) Olein. The ageing experiment was carried out at 85 ℃ and 115 ℃ for 1, 3, 5, 7 and 14 days. Several parameters were measured such as AC breakdown voltage, dielectric dissipation factor, relative permittivity, resistivity, viscosity, moisture and acidity throughout the ageing duration. Based on the study, it is found that there are no significant changes on the AC breakdown voltages and relative permittivities for both RBDPO and CO. At ageing temperature of 115℃, there are clear reduction trends of dielectric dissipation factor for CO and resistivities for most of RBDPO. On the other hand, no clear trends are observed for viscosities, moisture and acidities of RBDPO and CO throughout the ageing duration.

Korea's G7 Project - Broadband Integrated Service Digital Network(B-ISDN)- Completion Target Year 2001 (2001년 완성목표의 우리나라 G7 Project - 광대역종합통신망(B-lSDN) -)

  • 조규심
    • Journal of the Korean Professional Engineers Association
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    • v.27 no.2
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    • pp.42-54
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    • 1994
  • Nowadays communication network is steadily changing from a main body of telephone to a network which is creating new services such as multimedia etc. Jhis article introduces a bud of new service and new network which are able to sustain the above service. Including Korea, world advanced countries have been digitatizing their network with N-ISDN (narrowband ISDN) by e.g. 64K b/s and 1.5M b/s. New service by N-ISDN are extraordinary, though it can not solve all kinds of services. To solve these problems, a solving platform should be constituted. B-ISDN(broadband ISDN), as a platform has to be built to deal with almost all uinds of services, whatever the communication speed, communication mode or communication quality may be. Korea Telecommunication Authority(KTA) opened the narrowband ISDN(N-ISDN) at the end of 1993. This was one step previous opening of the broadband ISDN(B-ISDN) service scheduled to be rendered in 2001. B-ISDN, receiving and transmitting by far more information with high speed than the N-NSDN does, is one of G-7 Progects which the Korean government is driving foreward to raise the national scientific power to seven advan ced countries. This is also linked with an extremely high speed information network scheduled to be built by the Ministry of Communications in 2015.

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A Study on Dosimetric Characterization of Direct Yellow 12 Dye at High Radiation γ-Dose

  • Batool, Javaria;Shahid, Shaukat Ali;Ramiza, Ramiza;Akhtar, Nasim;Naz, Afshan;Yaseen, Maria;Ullah, Inam;Nadeem, Muhammad;Shakir, Imran
    • Bulletin of the Korean Chemical Society
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    • v.33 no.7
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    • pp.2265-2268
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    • 2012
  • Aqueous solution of oxygenated Direct yellow 12 dye has been evaluated spectrophotometrically as a possible gamma rays dosimeter. The neutral (pH-7), alkaline (pH-12.5) and acidic (pH-5.5) aqueous solution of the dye were prepared and exposed to various gamma doses. Absorption spectra of unirradiated and irradiated solutions were recorded at 400 nm peak. The increase in absorbance with the increase in irradiation dose was observed from 1 to 6 kGy. The stability response of the dye solution for different environmental conditions such as temperature (low & high), light and darkness were investigated during post irradiation storage for ten days. The dye solution showed high stability in darkness for the studied period. The optical density of the dye solution was found to be decreased at high temperature storage.

Repeated impact response of bio-inspired sandwich beam with arched and honeycomb bilayer core

  • Ahmad B.H. Kueh;Juin-Hwee Tan;Shukur Abu Hassan;Mat Uzir Wahit
    • Structural Engineering and Mechanics
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    • v.85 no.6
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    • pp.755-764
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    • 2023
  • The article examines the impact response of the sandwich beam furnished by a novel bilayer core as inspired by the woodpecker's head architecture under different repeatedly exerted low-velocity impact loadings by employing the finite element package, ABAQUS. The sandwich beam forms four essential parts comprising bottom and top carbon fiber reinforced polymer laminates encasing bilayer core made of laterally arched solid hot melt adhesive material and aluminum honeycomb. Impact loadings are implemented repeatedly with a steel hemisphere impactor for various impact energies, 7.28 J, 9.74 J, and 12.63 J. Essentially, the commonly concentrated stresses at the impact region are regulated away by the arched core in all considered cases thus reducing the threat of failure. The sandwich beam can resist up to 5 continual impacts at 7.28 J and 9.74 J but only up to 3 times repeated loads at 12.63 J before visible failure is noticed. In the examination of several key impact performance indicators under numerous loading cases, the proposed beam demonstrates favorably up to 1.3-11.2 higher impact resistance efficacies compared to existing designs, therefore displaying an improvement in repeated impact resistance of the new design.

Evaluation on the Lightning Breakdown Voltages of Palm Oil and Coconut Oil under Non-Uniform Field at Small Gap Distances

  • Thien, Yee Von;Azis, Norhafiz;Jasni, Jasronita;Kadir, Mohd Zainal Abidin Ab;Yunus, Robiah;Ishak, Mohd Taufiq;Yaakub, Zaini
    • Journal of Electrical Engineering and Technology
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    • v.11 no.1
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    • pp.184-191
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    • 2016
  • In recent years, there are a number of studies that have been carried out to explore the alternative for Mineral Oil (MO) as dielectric insulating fluid in transformers due to the increasing tight regulation on safety and environment. Vegetable oils have been identified as suitable candidate since it is biodegradable, non-toxic and high flash/fire points which ensure more in-service safety. Among the types of vegetable oils considered for transformers application are Palm Oil (PO) and Coconut Oil (CO). This paper presents an experimental study on the lightning breakdown voltages of PO and CO under non-uniform electric field based on needle-sphere electrodes configuration at 3 small gap distances. The type of PO used in this study is Refined Bleached and Deodorized Palm Oil (RBDPO) Olein. The main focus of this study is to examine the lightning breakdown performance of RBDPO and CO under different test conditions and assess its suitability as dielectric insulating fluid in transformers. The effect of voltage polarities (positive and negative) and testing methods (rising-voltage, up-and-down and multiple-voltage) were investigated. The data obtained from all tests were analysed by Weibull distribution in order to determine the withstand voltages for each type of oils. It was found that the breakdown voltages of RBDPO and CO are comparable with MO under positive lightning impulse. Under negative lightning impulse, the breakdown voltage of MO is slightly higher than RBDPO and CO. There is no significant effect of testing methods and voltage polarities on lightning breakdown voltages of RBDPO and CO. Based on the statistical analysis, it is found that the breakdown voltages of RBDPO and CO at 1% probability are slightly lower than MO.

Characterization of the first mitogenomes of the smallest fish in the world, Paedocypris progenetica, from peat swamp of Peninsular Malaysia, Selangor, and Perak

  • Hussin, NorJasmin;Azmir, Izzati Adilah;Esa, Yuzine;Ahmad, Amirrudin;Salleh, Faezah Mohd;Jahari, Puteri Nur Syahzanani;Munian, Kaviarasu;Gan, Han Ming
    • Genomics & Informatics
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    • v.20 no.1
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    • pp.12.1-12.7
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    • 2022
  • The two complete mitochondrial genomes (mitogenomes) of Paedocypris progenetica, the smallest fish in the world which belonged to the Cyprinidae family, were sequenced and assembled. The circular DNA molecules of mitogenomes P1-P. progenetica and S3-P. progenetica were 16,827 and 16,616 bp in length, respectively, and encoded 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and one control region. The gene arrangements of P. progenetica were identical to those of other Paedocypris species. BLAST and phylogenetic analyses revealed variations in the mitogenome sequences of two Paedocypris species from Perak and Selangor. The circular DNA molecule of P. progenetica yield a standard vertebrate gene arrangement and an overall nucleotide composition of A 33.0%, T 27.2%, C 23.5%, and G 15.5%. The overall AT content of this species was consistent with that of other species in other genera. The negative GC-skew and positive AT-skew of the control region in P. progenetica indicated rich genetic variability and AT nucleotide bias, respectively. The results of this study provide genomic variation information and enhance the understanding of the mitogenome of P. progenetica. They could later deliver highly valuable new insight into data for phylogenetic analysis and population genetics.

Optimized QCA SRAM cell and array in nanoscale based on multiplexer with energy and cost analysis

  • Moein Kianpour;Reza Sabbaghi-Nadooshan;Majid Mohammadi;Behzad Ebrahimi
    • Advances in nano research
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    • v.15 no.6
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    • pp.521-531
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    • 2023
  • Quantum-dot cellular automata (QCA) has shown great potential in the nanoscale regime as a replacement for CMOS technology. This work presents a specific approach to static random-access memory (SRAM) cell based on 2:1 multiplexer, 4-bit SRAM array, and 32-bit SRAM array in QCA. By utilizing the proposed SRAM array, a single-layer 16×32-bit SRAM with the read/write capability is presented using an optimized signal distribution network (SDN) crossover technique. In the present study, an extremely-optimized 2:1 multiplexer is proposed, which is used to implement an extremely-optimized SRAM cell. The results of simulation show the superiority of the proposed 2:1 multiplexer and SRAM cell. This study also provides a more efficient and accurate method for calculating QCA costs. The proposed extremely-optimized SRAM cell and SRAM arrays are advantageous in terms of complexity, delay, area, and QCA cost parameters in comparison with previous designs in QCA, CMOS, and FinFET technologies. Moreover, compared to previous designs in QCA and FinFET technologies, the proposed structure saves total energy consisting of overall energy consumption, switching energy dissipation, and leakage energy dissipation. The energy and structural analyses of the proposed scheme are performed in QCAPro and QCADesigner 2.0.3 tools. According to the simulation results and comparison with previous high-quality studies based on QCA and FinFET design approaches, the proposed SRAM reduces the overall energy consumption by 25%, occupies 33% smaller area, and requires 15% fewer cells. Moreover, the QCA cost is reduced by 35% compared to outstanding designs in the literature.

Genetic Variability Based on Randomly Amplified Polymorphic DNA in Kacip Fatimah (Labisia pumila Benth & Hook f) collected from Melaka and Negeri Sembilan States of Malaysia

  • Bhore, Subhash J.;Nurul, A.H.;Shah, Farida H.
    • Journal of Forest and Environmental Science
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    • v.25 no.2
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    • pp.93-100
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    • 2009
  • In Malaysia, Labisia pumila Benth & Hook f, popularly known as 'Kacip Fatimah' has been used traditionally to treat various elements of the woman's health in Malay community. The objective of this study was to develop randomly amplified polymorphic DNA (RAPD) based DNA markers for the identification of L. pumila and to distinguish its three varieties from each other. Total DNA from nine accessions of L. pumila was extracted by CTAB method and polymerase chain reactions (PCR) were carried out to amplify the segments of DNA using different primers to develop DNA barcode using RAPD technique. To find out variety-specific DNA marker/s, twenty different 10-mer primer sequences with annealing temperature from 36-$40^{\circ}C$ were evaluated in triplicate. Out of 20 random primers, two primers (OPA-1 and OPA-2/A10) were selected which produced reliable RAPD band patterns. To have DNA based handle, two RAPD amplification products were cloned and sequenced to determine the identity of the DNA. RAPD analysis using two random primers generated 72 discrete bands ranging in size 200 bp-3,000 bp. Fifty nine of these were polymorphic loci (82%) and thirteen were non-polymorphic loci (18%). A total of 32 bands polymorphic loci (72%) were amplified with primer OPA-1 and analyzed by cluster analysis and UPGMA (Unweighted Pair Group Method with Arithmetic) to present a dendogram depicting the degree of genetic relationship among nine accessions of L. pumila. Our results shows the reasonable genetic diversity among the L. pumila varieties and within varieties; and two RAPD marker sequences obtained could be used to identify L. pumila at species level.

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LTRE: Lightweight Traffic Redundancy Elimination in Software-Defined Wireless Mesh Networks (소프트웨어 정의 무선 메쉬 네트워크에서의 경량화된 중복 제거 기법)

  • Park, Gwangwoo;Kim, Wontae;Kim, Joonwoo;Pack, Sangheon
    • Journal of KIISE
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    • v.44 no.9
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    • pp.976-985
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    • 2017
  • Wireless mesh network (WMN) is a promising technology for building a cost-effective and easily-deployed wireless networking infrastructure. To efficiently utilize limited radio resources in WMNs, packet transmissions (particularly, redundant packet transmissions) should be carefully managed. We therefore propose a lightweight traffic redundancy elimination (LTRE) scheme to reduce redundant packet transmissions in software-defined wireless mesh networks (SD-WMNs). In LTRE, the controller determines the optimal path of each packet to maximize the amount of traffic reduction. In addition, LTRE employs three novel techniques: 1) machine learning (ML)-based information request, 2) ID-based source routing, and 3) popularity-aware cache update. Simulation results show that LTRE can significantly reduce the traffic overhead by 18.34% to 48.89%.