• 해양환경안전학회지
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• 제24권5호
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• pp.537-544
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• 2018

본 연구에서는 해수 중 유체력에 의한 인공어초의 활동 및 전도를 평가하기 위한 간편하고 효율적인 사용자 중심의 전산 프로그램(SCAR program)을 개발하였다. 개발된 전산 프로그램은 인공어초의 안정성 평가 공식인 모리슨(Morison) 식을 기반으로 델파이(Delphi) 코드 및 그래픽 사용자 인터페이스(GUI, Graphic User Interface) 방식을 적용하였다. 개발된 SCAR 프로그램은 학부 및 대학원 과정, 실제 현장 전문가들의 수산구조물(인공어초 또는 수중구조물 등) 설계 및 안정성 평가에 널리 적용 가능할 것으로 판단된다.

• Journal of Industrial and Engineering Chemistry
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• 제67권
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• pp.469-477
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• 2018

We describe surgical sutures enabled with the local, sustained delivery of a TGF-${\beta}$ inhibitory drug, tranilast. To fabricate drug-delivery sutures, we separately prepared a tranilast-loaded strand using poly (lactic-co-glycolic acid), which was then physically braided with a surgical suture already in clinical use. By this method, the drug-delivery sutures maintained the mechanical strength and allowed the modulation of drug release profiles by simply altering the tranilast-loaded strand. The drug-delivery sutures herein released tranilast for up to 14 days. When applied to animal models, scarring was indeed reduced with diminished TGF-${\beta}$ expression and fibroblast numbers during the entire 21 day testing period.

• 원예과학기술지
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• 제31권6호
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• pp.756-763
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• 2013

당근의 기계적인 제모 시 발생하는 종자의 손실과 발아 시의 문제점을 개선한, 고품질의 당근 종자 생산을 위한 단모종자 당근 품종 육성에 이용할 분자표지를 개발하기 위하여 본 연구를 수행하였다. 본 연구에서는 2008년도부터 2013년도까지 단모종자 표현형 CT-SMR 616 OP 389-1 개체와 장모종자 표현형 CT-SMR 616-33 개체를 자가수분하여 세대 진전된 당근 계통들을 종자모 형질 관련 RAPD-SCAR 분자표지를 개발하는데 이용하였다. 이들 계통의 종자모 길이를 현미경을 이용하여 분석하였으며, 분석된 결과를 바탕으로 계통을 세대진전 시켜, 분자표지 다형성과도 비교분석하였다. 분자 표지 개발을 위하여 세대가 고정되었다고 판단되는 2011년 계통을 대상으로 80개의 random primer를 이용한 RAPD 분석을 통해 12개의 개체간 뚜렷한 차이를 보이는 종자모 형질 관련 특이적 band를 확인하였다. RAPD-SCAR 분자표지 개발을 위해 확인된 이들 특이적 band의 염기서열 분석을 통해 SCAR primer를 작성하였으며 각 SCAR primer는 24-28mer 크기로 3조합 이상 작성하였다. 분석 결과 작성된 SCAR primer 중 $SCA2_{1.2}$가 단모종자 표현형 계통에서만 특이적으로 증폭되는 것을 확인하였다. 이 $SCA2_{1.2}$ 분자표지의 정확성을 검증하기 위해 2012년도 계통과 2013년도 계통을 이용하여 재검증하였으며, 그 결과 개발된 SCAR 분자표지는 단모종자와 장모종자 계통을 구분할 수 있는 충분한 다형성을 제공하였다. 따라서 본 연구에서 개발된 SCAR 분자표지, $SCA2_{1.2}$는 당근의 단모종자 품종 육성 연구에 충분히 활용가능 할 것으로 기대된다.

• The Plant Pathology Journal
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• 제22권1호
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• pp.36-45
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• 2006

Twenty isolates of Didymella bryoniae were isolated from infected cucurbit plants in various growing areas of southern Korea in 2001 and 2002. Random Amplified Polymorphic DNA (RAPD) group [RG] I of D. bryoniae was more virulent than RG IV to watermelon. Virulence of the RG I isolate was strong to moderate to cucumber, whereas that of the RG IV varied from strong, moderate to weak. Two hundred seventy-three amplified fragments were produced with 40 primers, and were analyzed by a cluster analysis using UPGMA method with an arithmetic average program of NTSYSPC. At the distance level of 0.7, two major genomic DNA RAPD groups were differentiated among 20 isolates. The RG I included 7 isolates from watermelon and one isolate from melon, whereas the RG IV included 12 isolates from squash, cucumber, watermelon and melon. Amplification of internal transcribed spacer (ITS) region and small subunit rRNA region from the 20 isolates yielded respectively a single fragment. Restriction pattern with 12 restriction enzymes was identical for all isolates tested, suggesting that variation in the ITS and small subunit within the D. bryoniae were low. Amplification of the genomic DNAs of the tested isolates with the sequence characterized amplified regions (SCAR) primer RG IF-RG IR specific for RG I group resulted in a single band of 650bp fragment for 8 isolates out of the 20 isolates. Therefore, these 8 isolates could be assigned into RG I. The same experiments done with RG IIF-RG IIR resulted in no amplified PCR product for the 20 isolates tested. An about 1.4 kb-fragment amplified from the RG IV isolates was specifically hybridized with PCR fragments amplified from genomic DNAs of the RG IV isolates only, suggesting that this PCR product could be used for discriminating the RG IV isolates from the RG I isolates as well other fungal species.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2003년도 춘계 수산관련학회 공동학술대회발표요지집
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• pp.397-399
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• 2003

전세계적으로 연안환경에서의 중금속 오염을 모니터링 하는 데에는 Mussel Watch Program을 중심으로 담치류(mussel)와 굴(oyster) 등이 지표종(indicator species)으로 널리 사용되어 왔다(O'Conner et al.,1994). 그러나 남극에는 이 두종이 서식하고 있지 않으며, 현재까지 연구에 따르면 남극큰띠조개와 남극가리비(Adamussium cozbecki)가 담치류에 상응하는 유용한 생물지표종으로 사용될 수 있다고 알려져 있다(안 등, 1996, 2001 ; SCAR, 1996 ; Nigro et al., 1992). (중략)

• Archives of Plastic Surgery
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• 제42권2호
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• pp.223-225
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• 2015

The closure of any circular or asymmetric wound can result in puckering or an excess of tissue known as a 'dog ear'. Understanding the mechanism of dog ear formation is a fundamental requirement necessary to facilitate an appropriate treatment. Many solutions have been reported in the literature, but in all cases, the correction entails the extension of the scar and the sacrifice of the dermal plexus. Here, we propose a novel technique of dog ear correction by using a three-bite suture that sequentially pierces the deep fascial plane and each dog ear's margin, thus allowing for flattening the dog ear by anchoring the over-projecting tissue to the deep plane. The three-bite technique proved to be a fast, easy, and versatile method of immediate dog ear correction without extending the scar, while maintaining a full and complete local skin blood supply.

• 대한후두음성언어의학회지
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• 제33권3호
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• pp.166-171
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• 2022

Background and Objectives Vocal fold (VF) scar is known to be the most common cause of dysphonia after laryngeal microsurgery (LMS). Steroids reduce postoperative scar formation by inhibiting inflammation and collagen deposition. However, the clinical evidence of whether steroids are helpful in reducing VF scar formation after LMS is still lacking. The purpose of this study is to determine whether intralesional VF steroid injection after LMS helps to reduce postoperative scar formation and voice quality. Materials and Method This study was conducted on 80 patients who underwent LMS for VF polyp, Reinke's edema, and leukoplakia. Among them, 40 patients who underwent VF steroid injection after LMS were set as the injection group, and patients who had similar sex, age, and lesion size and who underwent LMS alone were set as the control group. In each group, stroboscopy, multi-dimensional voice program, Aerophone II, and voice handicap index (VHI) were performed before and 1 month after surgery, and the results were statistically analyzed. Results There were no statistically significant differences in the distribution of sex, age, symptom duration, occupation and smoking status between each group. Both groups consisted of VF polyp (n=21), Reinke's edema (n=11), and leukoplakia (n=9). On stroboscopy, the lesion disappeared after surgery, and the amplitude and mucosal wave were symmetrical on both sides of the VFs in all patients. Acoustic parameters and VHI significantly improved after surgery in all patients. However, there was no significant difference between the injection and control group in most of the results. Conclusion There was no significant difference in the results of stroboscopy, acoustic, aerodynamic, and subjective evaluation before and after surgery in the injection group and the control group.

• 한방안이비인후피부과학회지
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• 제29권1호
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• pp.93-102
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• 2016

Objective : This study performed to evaluate that duration of acne scars effects on Qualitative Global Acne Scarring Grading System(QGASC) grade changing and treatment duration. Also this study performed to evaluate the relationship between dysmenorrhea and acne scars, and the effects of each treatment on treatment duration and Korean Acne Grading System(KAGS) and QGASC grade.Methods : 95 patients with acne scars were understood by medical records and case photos, and we evaluated pre and post acne scar grade based on KAGS and QGASC. We analyzed data by SPSS 22 for windows program, and figured out the relation between duration of acne and QGASC grade and treatment duration by Spearman correlation analysis, and performed Mann-Whitney U-test to figure out the relation between dysmenorrhea and QGASC grade. We performed Wilcoxon test to evaluate that treatment of acne scars effects on KAGS grade and QGASC grade, and performed Kruskal-Wallis test to figure out the difference of each treatment which effects on KAGS grade and QGASC grade, and performed Post-hoc analysis to figure out the group comparison.Results & Conclusions : QGASC grade depending on duration of acne was statistically significance. Subcision and Microneedle Therapy System(MTS) and pharmacopuncture were significantly improved acne scars. Other treatments were significantly more effective than subcision and pharmacopuncture combined treatment. Treatment duration of Subcision and MTS combined treatment was significantly most shortest than other treatment.

• Mycobiology
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• 제47권2호
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• pp.200-206
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• 2019

Allelic differences in A and B mating-type loci are a prerequisite for the progression of mating in the genus Pleurotus eryngii; thus, the crossing is hampered by this biological barrier in inbreeding. Molecular markers linked to mating types of P. eryngii KNR2312 were investigated with randomly amplified polymorphic DNA to enhance crossing efficiency. An A4-linked sequence was identified and used to find the adjacent genomic region with the entire motif of the A locus from a contig sequenced by PacBio. The sequence-characterized amplified region marker $7-2_{299}$ distinguished A4 mating-type monokaryons from KNR2312 and other strains. A BLAST search of flanked sequences revealed that the A4 locus had a general feature consisting of the putative HD1 and HD2 genes. Both putative HD transcription factors contain a homeodomain sequence and a nuclear localization sequence; however, valid dimerization motifs were found only in the HD1 protein. The ACAAT motif, which was reported to have relevance to sex determination, was found in the intergenic region. The SCAR marker could be applicable in the classification of mating types in the P. eryngii breeding program, and the A4 locus could be the basis for a multi-allele detection marker.

• 아시안잔디학회지
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• 제24권2호
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• pp.131-137
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• 2010

최근 국내 일부 골프장에서는 그린을 공사하지 않고 신품종으로 교체할 수 있는 인터씨딩에 대한 관심이 높아지고 있다. 하지만 이에 대한 어떤 연구나 평가 방법 등이 발표되어 있지 않다. 따라서 본 연구에서는 분자생물학적 기법을 이용한 인터씨딩률을 조사하여 그 실효성을 평가하고자 수행하였다. 우정힐스골프장은 기존 품종인 Penncross에 Penn A-4 품종을 1회 인터씨딩하였고, 레이크사이드골프장은 기존품종인 penncross에 CY-2 품종을 3회 인터씨딩하였다. SCAR marker를 이용한 인터씨딩율은 1회 인터씨딩한 우정힐스골프장은 치환율이 20%였고, 3회 인터씨딩한 레이크사이드골프장은 37.3%였다. 1회와 3회 인터씨딩에 의한 치환율의 차이는 15.3% 정도였다. 하지만 3회 인터씨딩한 지역을 답압별로 세부하여 1회 인터씨딩율과 비교하여 보면, 답압이 가장 적게 이루어진 지역은 47.2%로 대략 50%정도가 치환되었다. 이는 1회의 치환률과 비교하여 173%이상 치환률이 증가하였다. 따라서 인터씨딩은 1회 실시하는 것보다는 3회 실시한 경우에 치환률이 훨씬 높아, 향후 인터씨딩을 실시할 경우에는 다년간의 계획을 수립하여 인터씨딩을 실시하는 것이 효과적일 것으로 판단된다. 따라서 본 연구에서는 소량의 시료만을 이용한 인터씨딩률에 대한 분석이 가능하였고, 유전분석을 이용한 인터씨딩율 조사를 통해 현재 시행하고 있는 인터씨딩 방법에 대한 기술 개선에 많은 도움이 있을 것으로 판단된다.