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http://dx.doi.org/10.7235/hort.2013.13106

Development of RAPD-SCAR Molecular Marker Related to Seed-hair Characteristic in Carrot  

Shim, Eun-Jo (Department of Horticultural Biotechnology, Kyung Hee University)
Park, Sung-Kwan (Department of Horticultural Biotechnology, Kyung Hee University)
Oh, Gyu-Dong (Department of Horticultural Biotechnology, Kyung Hee University)
Jun, Sang-Jin (Breeding Research Institute, Carrotop Seed Co.)
Park, Young-Doo (Department of Horticultural Biotechnology, Kyung Hee University)
Publication Information
Horticultural Science & Technology / v.31, no.6, 2013 , pp. 756-763 More about this Journal
Abstract
Mechanical hair removal of carrot seed causes seed injuries and suppresses the germination in carrot cultivation. This study was performed to develop molecular markers for breeding high quality cultivars with short-hair seed. To meet this objective, random amplified polymorphic DNA (RAPD)-sequence characterized amplified region (SCAR) markers specifically linked to seed-hair characteristic were identified using CT-SMR 616 OP 389-1 line with short-haired seed and CT-SMR 616 OP 616-33 line with long-haired seed, bred by self-pollination for 6 years from 2008 to 2013, as parents. After seed hair lengths of these lines were analyzed using microscope, next generations were advanced and compared with the molecular markers polymorphism. From RAPD analysis using fixed lines in 2011, twelve RAPD primers showing polymorphic bands specific between the two lines were identified from 80 random primers. To develop RAPD-SACR marker, SCAR primers were designed based on sequence analysis of these specific RAPD bands and more than three combinations of primers were tested. As a result, it was found that the $SCA2_{1.2}$ amplified single polymorphic band from short-haired seed line. To confirm this result, $SCA2_{1.2}$ marker was retested by applying to the 2012 and 2013 progenies. Finally, it was concluded that the developed $SCA2_{1.2}$ marker distinguished short-haired line from long-haired seed line. Therefore, SCAR marker, $SCA2_{1.2}$ is expected to be utilized for breeding of the short-haired seed cultivars.
Keywords
breeding program; long-hair seed; polymorphism; short-hair seed;
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