• 한국연초학회지
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• 제15권1호
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• pp.34-48
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• 1993

This experiment were conducted to investigate heterosis and combining ability for several mutant characters by analyzing dialled crosses of flue-cured tobacco. In a dialled cross of 3 flue-cured varieties and the mutant line 83H -5, the heterosis was somewhat higher in Fl than in F2. For growth character, the heterosis was 0.28-6.03% in plant height, leaf number, leaf shape index and yield, and was 43.2% for bacterial wilt disease index. The mutant line 83H-5 showed significantly negative GCA effect for plant height, leaf width and bacterial wilt disease index in Fl and F2, leaf length in F2, and positive GCA effect for total alkaloids, total nitrogen in Fl and days to flower in F2, respectively. Specific combining ability(SCA) in 83H-5 x Hicks was significant in negative effect for leaf length(F2), number of leaves(F2), leaf shape(F1, F2), bacterial wilt(F2) and alkaloids(F1), and in 83H-5 x NC 2326 in positive effect for leaf length(F1, F2) and leaf width(F2), and for 83H-5 x NC 82 in positive effect for plant height(F1, F2) and leaf width(F2), and for 83H-5 x NC 82 in Positive effect for Plant height(F1, F2), leaf length(F2) and yield(F1, F2).

• 생명과학회지
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• 제10권2호
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• pp.177-181
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• 2000

This study was conducted to estimate the genetic parameters such as combining ability, heritabilities and correlation coefficients forsome agronomic characters by 6$\times$6 dialled crosses of barley. The results obtained were summarized as follows. Mean squares of general combining ability(GCA) and specific combining ability(SCA) were significant for all characters observed. Mean squares of general combining ability for all characters were expressed as higher values than those of specific combining ability. Jecheon #5 showed the high general combining ability effects for culm length, and Waegwanpimack #1 showed the highest general combining ability effects for tiller number. Cygne also showed the high general combining ability effects for spike length and 1,000 grains weight. Furthermore, Plumage was expressed with the highest general combining ability effects for spike length and 1,000 grains weight. Furthermore, Plumage was expressed with the highest general combining ability effects for number of grains per spike. In specific combining ability effects, Samdugjeonbug # 45$\times$Plumage showed the high value for culm length, and Suwon #6 $\times$Waegwanpimack #1$\times$ was expressed with the highest specific combining ability effects for tiller number. Jecheon #5$\times$Plumage also showed the high specific combining ability effects for spike length and number of grains per spike. Suwon #6$\times$Cygne showed the high specific combining ability effects for 1,000 grains weight. The heritabilities of spike length an number of grains per spike were over 90%. In correlation coefficients among characters, relationships between culm length and tiller number, culm length and spike length, culm length and 1,000 grains weight, and spike length and 1,000 grains weight showed highly positively correlation.

• 미생물학회지
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• 제24권4호
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• pp.341-351
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• 1986

항생제에 대하여 다중 저항성을 갖는 Shaphylococcus aµreus D-H-1으로부터 chloram phenicol(Cm) 저항성 유전자를 가진것으로 확인된 R-plasmid(pSBK203, 2.5MdaJ.)를 분리하여 Bacillus subtilis BD 170 내에셔 발현시켰으며 이 Cm 저항성 유전자를 cloning하기 위하여 pSBK203상의 제한효소 인식부위를 결정하였다. pSBK203 상의 TaqI 부분 절편 0.3 kb을 pBD9 CZaI 인식부위내에 삽입하여 얻은 재조합 plasmid(pTQ16)와 TaqI부 분절편과 O.lkb 엇갈럼이 있는 RsaI 단일절떤(1. 3 kb} pBR322의 Seal 인식부위에 삽입하여 얻은 재조합 p plasmid(pHW20)에서 Cm 저항성이 획득되었다. pBD9 및 pBR322 상에 삽입된 두 절펀내에 Hinf, Taq I 빛 BglII의 제한효소 인식부위가 존재하였으며 이들 중 행III 인식부위에 의하여 Cm 저항성이 불활성 되었다.

• 한국지반공학회논문집
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• 제17권6호
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• pp.173-180
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• 2001

지진동으로 인한 개착식터널의 동적 거동특성을 파악하기 위해 Prototype터널 폭이 약 14.2m, 높이가 약 8.5m를 1/40sca1e로 축소제작한 모형체의 진동대 실험을 수행하였으며 본 실험에서는 터널의 토피고 및 원지반의 경사각에 따른 영향을 분석하였으며 이를 동적수치해석결과와 비교 분석하였다. 진동대 실험결과 터널의 토피고가 증가할수록 터널에 발생되는 응력 및 가속도는 감소하는 것으로 나타났으며 이는 터널의 설치심도가 증가할수록 주변지반의 구속효과가 증가하기 때문인 것으로 판단된다. 또한 원지반의 경사가 완만할수록 터널에 발생되는 응답치는 증가하는 경향을 보이고 있는데 이는 지진동으로 인한 주변지반의 구속효과가 원지반의 영향 보다는 되메움토사의 영향을 지배적으로 받기 때문인 것으로 판단된다. 또한 진동대실험결과 및 상시조건의 구조해석결과와 비교하면 터널의 토피고가 터널직경의 1배(1D)이상 확보되는 조건일 경우, 지진동이 터널에 미치는 영향이 적은 것으로 나타났다.

• Molecules and Cells
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• 제38권6호
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• pp.548-561
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• 2015

By combining conventional single cell analysis with flow cytometry and public database searches with bioinformatics tools, we extended the expression profiling of thymic stromal cotransporter (TSCOT), Slc46A2/Ly110, that was shown to be expressed in bipotent precursor and cortical thymic epithelial cells. Genome scale analysis verified TSCOT expression in thymic tissue- and cell type- specific fashion and is also expressed in some other epithelial tissues including skin and lung. Coexpression profiling with genes, Foxn1 and Hoxa3, revealed the role of TSCOT during the organogenesis. TSCOT expression was detected in all thymic epithelial cells (TECs), but not in the $CD31^+$endothelial cell lineage in fetal thymus. In addition, ABC transporter-dependent side population and Sca-$1^+$ fetal TEC populations both contain TSCOT-expressing cells, indicating TEC stem cells express TSCOT. TSCOT expression was identified as early as in differentiating embryonic stem cells. TSCOT expression is not under the control of Foxn1 since TSCOT is present in the thymic rudiment of nude mice. By searching variations in the expression levels, TSCOT is positively associated with Grhl3 and Irf6. Cytokines such as IL1b, IL22 and IL24 are the potential regulators of the TSCOT expression. Surprisingly, we found TSCOT expression in the lung is diminished in lung cancers, suggesting TSCOT may be involved in the suppression of lung tumor development. Based on these results, a model for TEC differentiation from the stem cells was proposed in context of multiple epithelial organ formation.

• 한국작물학회지
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• 제46권4호
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• pp.296-302
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• 2001

Winter cereals are acclimated during wintering, and thereafter their freezing resistance is increased. In order to analyze inheritance and heritabilities for photoinhibition of photosynthesis by high light intensity under low temperature, and to evaluate the relationship between low temperature-induced photoinhibition and winter survival, 4 parental half diallel crosses were used. The detached leaves of 7-8cm long from plants grown for 35 and 55 days were placed on wet filter paper and placed in trays at 5$^{\circ}C$ cold room with 1,200 $\mu$mol $m^{-2}$ $s^{-1}$ PPFD. Chlorophyll fluorescence was measured with a chlorophyll fluorescence system after dark adaptation for 30 min. The Fv/Fm of 35day old plants was reduced from 0.714 in the control leaves to 0.409 and 0.368 following photoinhibitory treatment of 6h and 8h and the CVs were increased from 0.8% to 22.2-22.3%. The Fv/Fm of 55-day old plants was reduced from 0.775 in the control leaves to 0.485 and 0.439 following photoinhibitory treatment of 10h and 12h, respectively. According to half diallel cross analysis, Reno and Dongbori 1 (highly resistant to photoinhibition) was dominant, but Oweolbori (susceptible to photoinhibition) was recessive, and photoinhibition showed partial dominance with highly additive gene action. Dongbori 1 showed the greatest GCA effects for photoinhibition, and GCA/SCA ratios (8.7-22.3 times) indicated that the additive variance for the character was more important. Winter survival in barley crosses was positively correlated with resistance to photoinhibition and significantly fitted by linear regression ($R^2$=0.751$^{**}$-0.779$^{**}$). The chlorophyll fluorescence measured by Fv/Fm has been found to be highly inheritable and very useful in evaluating relative levels of freezing resistance in barley.ley.

• Molecules and Cells
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• 제25권2호
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• pp.216-223
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• 2008

It has been reported that bone marrow (BM)-side population (SP) cells, with hematopoietic stem cell activity, can transdifferentiate into cardiomyocytes and contribute to myocardial repair. However, this has been questioned by recent studies showing that hematopoietic stem cells (HSCs) adopt a hematopoietic cell lineage in the ischemic myocardium. The present study was designed to investigate whether BM-SP cells can in fact transdifferentiate into functional cardiomyocytes. Phenotypically, BM-SP cells were $19.59%{\pm}9.00\;CD14^+$, $8.22%{\pm}2.72\;CD34^+$, $92.93%{\pm}2.68\;CD44^+$, $91.86%{\pm}4.07\;CD45^+$, $28.48%{\pm}2.24\;c-kit^+$, $71.09%{\pm}3.67\;Sca-1^+$. Expression of endothelial cell markers (CD31, Flk-1, Tie-2 and VEGF-A) was higher in BM-SP cells than whole BM cells. After five days of co-culture with neonatal cardiomyocytes, $7.2%{\pm}1.2$ of the BM-SP cells expressed sarcomeric ${\alpha}$-actinin as measured by flow cytometry. Moreover, BM-SP cells co-cultured on neonatal cardiomyocytes fixed to inhibit cell fusion also expressed sarcomeric ${\alpha}$-actinin. The co-cultured BM-SP cells showed neonatal cardiomyocyte-like action potentials of relatively long duration and shallow resting membrane potential. They also generated calcium transients with amplitude and duration similar to those of neonatal cardiomyocytes. These results show that BM-SP cells are capable of functional cardiomyogenic differentiation when co-cultured with neonatal cardiomyocytes.

• 한국지리정보학회지
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• 제18권1호
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• pp.1-12
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• 2015

본 연구에서는 기상관측소의 적설심 자료와 Terra MODIS 위성영상을 이용하여, 3가지 대표시설물(원예특작물시설, 축사, 건축물)에 대한 적설 설계기준 취약지역을 분석하였다. 현재 적용되고 있는 시설물의 적설 설계강도 기준을 평가하고 폭설 취약지역을 분석하기 위해, 과거 12년간(2001~2012년) 수집된 Terra MODIS 위성영상에서 추출된 적설면적과 적설심 자료를 이용하여 전국 적설심 분포도를 작성하였다. 또한 전국지역별 원예특작물시설의 설계기준 적설심(cm), 축사 설계기준 적설하중($kg/m^2$), 건축물 설계기준 적설하중($kN/m^2$) 자료를 수집하여, MODIS 위성영상에 의해 구축된 적설심 분포도와 비교하여 적설 설계강도 기준 취약지역을 분석한 결과 전라도, 충청도 및 강원도 지역이 취약한 것으로 분석되었다.

• 한국작물학회지
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• 제58권2호
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• pp.91-106
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• 2013

Exserohilum turcicum is considered serious destructive disease of maize (Zea mays L.) in North Korea. This study aimed to understand genetic inheritance and combining ability of newly bred lines of maize tolerant to E. turcicum by diallel crosses. Three diallel sets for two different ecological regions and one agronomic trait; eastern (E), northern (N) and stay green (SG) involving 29 inbred lines were tested in eight locations of 2000 and 2001. E. turcicum infections were under natural conditions, respectively. Lines used were selected for high yield potential in test crosses with good agronomic traits and tolerance to biotic and abiotic stresses. Selection for race specific high resistance to biotic stresses was avoided to select quantitatively inherited genes. Host plant responses to E. turcicum were rated on a scale of 1 (highly tolerant) to 9 (highly susceptible). Highly significant variations were recorded in all trials. General combining ability (GCA) mean square was roughly twice that of specific combining ability (SCA). The genotype (G) by environment (E) interaction was highly significant. The overall results of genetic studies in three diallel sets show that genetic control for inbred tolerance to E. turcicum is polygenic and quantitatively inherited. New inbreds; E-3, N-1 and SG-4 confer better tolerance to E. turcicum than the widely used inbreds; Mo17, and B73. Proper use of genetic information from this study shall increase of corn production under high E. turcicum infection in the Far Eastern Regions of Korea and China.

• 한국잠사곤충학회지
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• 제28권2호
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• pp.28-34
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• 1986

The general combining ability (GCV), the specific combining ability (SCA) and the reciprocal combining ability (RCA) effects wereobtained by 8$\times$8 diallel crosses of the silkworms with four Japanese races and four Chinese races, total eight lines. The result are as follows ; 1. The general combining ability (GVA) effects appeared high significant level in the total and the fifth instar periods (TP, FP), a female and a male cocoon weights (FW, MW), a female and a male cocoon layer weights (FL, ML) and a female and a male cocoon layer ratios (FR, MR). Only the reciprocal combining ability (RCA) effects appeared high significant level in the total and the fifth instar periods (TP, FP). 2. The Japanese original silkworm lines varied in the general combining ability effects from -0.864 to 0.578, and the Chinese original silkworm lines did in ones from -0.570 to 1.018. 3. The specific combining ability effects of the silkworm lines made in order of the crossing types of JL (Japanese lines) $\times$CL (Chinese lines)>CL$\times$JL>JL$\times$JL> CL$\times$CL in a total cocoon weights and a cocoon layer weights. 4. The reciprocal combining ability effects of the silkworm lines was in order of the crossing types of JL$\times$CL>JL$\times$JL> CL$\times$CL>CL$\times$JL in a total cocoon weights and cocoon layer weights.