• 디지털콘텐츠
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• 9호통권64호
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• pp.101-105
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• 1998

이 코너는 데이터베이스 관련 표준화 활동중에서 ISO와 IEC의 합동기술위원회(JTC1), SC32 Data Management and Interchange(데이터 관리 및 교환)의 표준화 활동과 데이터베이스 단체표준을 제정하는 데이터베이스 표준총회의 활동을 중심으로 매월 소개하고자 한다. 이번호는 지난 7월 7일부터 11일까지 호주 브리스베인에서 열린 JTC1/SC32 총회의 회의 내용과 제3차 JTC1/SC32 전문위원회 회의 내용, 제6차 데이터베이스 표준화 전문위원회 회의 내용을 게재한다.

• IMMUNE NETWORK
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• 제4권1호
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• pp.7-15
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• 2004

Background: The heat shock proteins (HSPs) play an important role in cellular protection mechanisms against physical or chemical stresses. In this study scFv antibodies specific for human HSP70.1 were isolated from a semi-synthetic human scFv library with the ultimate goal of developing anti-HSP70.1 intracellular antibody (intrabody) that may offer an attractive alternative to gene targeting to study the function of the protein in cells. Methods: A semi-synthetic human scFv display library ($5{\times}10^{8}$ size) was constructed using pCANTAB-5E vector and the selection of the library against bacterially expressed recombinant human HSP70.1 was attempted by panning. Results: Three positive clones specific for recombinant HSP70.1 were identified. All three clones used $V_{H}$ subgroup III. On the other hand, $V_{L}$ of two clones belonged to the kappa light chain subgroup I, but the other utilized $V_{k}$ subgroup IV Interestingly, these scFv molecules specifically reacted to the recombinant HSP70.1, yet failed to recognize native HSP70 induced in U937 human monocytic cells by heat treatment. Conclusion: Our results indicated that affinity selection of an scFv phage display library using recombinant antigens produced in E. coli might not guarantee the isolation of scFv antibody molecules specific for a native form of the antigen. Therefore, the source of target antigens needs to be chosen carefully in order to isolate biofunctional antibody molecules.

• 한국방송∙미디어공학회:학술대회논문집
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• 한국방송∙미디어공학회 2020년도 추계학술대회
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• pp.208-209
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• 2020

ISO/IEC JTC 1/SC 29(멀티미디어부호화) WG 11(MPEG)에서는 미디어 산업 전반에 대한 표준화를 다루고 있으며 해당 산업과 연관된 다양한 기관들과의 교류가 진행되고 있으나, 작업반(WG)이란 한계와 현행 ISO/IEC 규정에 의해 다양한 표준화 단체들과의 교류에 많은 제한이 있었다. 이에, 2019년 7월 관련 임시 작업반(AhG)이 설립되어 구조조정에 대한 논의를 진행했으며, 2020년 7월에 열린 37차 JTC 1/SC 29 총회에서 기존 WG 11을 폐지하고 새로운 작업반을 신설했다. 이에 본 논문은 JTC 1/SC 29/WG 10(MPEG) 구조조정 결과 및 신설 작업반의 구조와 표준화 동향을 알아본다.

• 한국세라믹학회지
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• 제53권5호
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• pp.500-505
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• 2016

Scandia-stabilized zirconia co-doped with $CeO_2$ is a promising electrolyte for intermediate temperature SOFC, but still shows rapid degradation during a long-term operation. In this study, $CeO_2$ (1 mol%) as a stabilizer is partially substituted with lanthanum oxides ($M_2O_3$, M=Yb, Gd, Sm) to stabilize a cubic phase and thus durability in reducing atmosphere. 0.5M0.5Ce10ScSZ electrolytes were prepared by solid state reaction and sintered at $1450^{\circ}C$ for 10 h to produce dense ceramic specimens. With addition of the lanthanum oxide, 0.5M0.5Ce10ScSZ showed lower degradation rates than 1Ce10ScSZ. Since $Gd_2O_3$ showed the highest ionic conductivity among the co-dopants, an electrolyte-supported cell with 0.5Gd0.5Ce10ScSZ was prepared to compare its long-term performance with that of 1Ce10ScSZ-based cell. Maximum power density of 0.5Gd0.5Ce10ScSZ-based cell was degraded by about 2.3% after 250 h, which was much lower than 1Ce10ScSZ-based cell (4.2%).

• IMMUNE NETWORK
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• 제12권1호
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• pp.33-39
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• 2012

Background: Therapeutic approaches using monoclonal antibodies (mAbs) against complement regulatory proteins (CRPs:i.e.,CD46,CD55 and CD59) have been reported for adjuvant cancer therapy. In this study, we generated a recombinant 1E8 single-chain anti-CD59 antibody (scFv-Fc) and tested anti-cancer effect.by using complement dependent cytotoxicity (CDC). Methods: We isolated mRNA from 1E8 hybridoma cells and amplified the variable regions of the heavy chain (VH) and light chain (VL) genes using reversetranscriptase polymerase chain reaction (RT-PCR). Using a linker, the amplified sequences for the heavy and light chains were each connected to the sequence for a single polypeptide chain that was designed to be expressed. The VL and VH fragments were cloned into the pOptiVEC-TOPO vector that contained the human CH2-CH3 fragment. Then, 293T cells were transfected with the 1E8 single-chain Fv-Fc (scFv-Fc) constructs. CD59 expression was evaluated in the prostate cancer cell lines using flow cytometry. The enhancement of CDC effect by mouse 1E8 and 1E8 scFv-Fc were evaluated using a cytotoxicity assay. Results: The scFv-Fc constructs were expressed by the transfected 293T cells and secreted into the culture medium. The immunoreactivity of the secreted scFv-Fc construct was similar to that of the mouse 1E8 for CCRF-CEM cells. The molecular masses of 1E8 scFv-Fc were about 120 kDa and 55 kDa under reducing and non-reducing conditions, respectively. The DNA sequence of 1E8 scFv-Fc was obtained and presented. CD59 was highly expressed by the prostate cancer cell line. The recombinant 1E8 scFv-Fc mAb revealed significantly enhanced CDC effect similar with mouse 1E8 for prostate cancer cells. Conclusion: A 1E8 scFv-Fc construct for adjuvant cancer therapy was developed.

• 대한의생명과학회지
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• 제9권3호
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• pp.111-121
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• 2003

Recombinant single chain Fv (scFv) antibodies offer many advantages over mouse monoclonal antibodies such as faster clearance from blood, improved tumor localization, reduced human anti-mouse antibody (HAMA) response, and the availability to manipulate the scFv through genetic approaches. The recombinant phage display was constructed using lym-l hybridoma cells as a source of genetic starting material. mRNA was isolated from the corresponding antibodies hybridoma cells. VH and VL cDNA were amplified with RT-PCR and linked with ScFv by linker DNA to form ScFv DNA, which then were inserted into phagemid pCANTAB5E. The phage of positive clones selected with tube containing raji lymphoma cell and infected by competent E. coli HB2151 to express soluble scFv. The scFv lym-l was secreted into the cytosol and culture supernatant and shown to be of expected size (approximately 32 kDa) by western blot. An active scFv lym-l could be produced in E. coli with soluble form and high yield from hybridoma cell line, using phage display system. Immunoreactivity indicated that scFv lym1 showed a potential biding affinity against the raji lymphoma cell as its parental antibody (intact lym-l Ab).

• 한국주조공학회지
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• 제29권5호
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• pp.213-219
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• 2009

Optimum conditions for production of semi-solid Al-Zn-Mg alloy billets was carried out by the Taguchi design method. And, Al-Zn-Mg alloy billets contained Sc (free, 0.1 and 0.3 mass %) were fabricated at optimum conditions. Evolution of microstructure in semi-solid state was investigated through various liquid fractions, holding times and holding temperatures. The Al-Zn-Mg alloy billets reheated at $615^{\circ}C$ during 30min are grain growth and it was fractured due to increasing liquid fraction before quenching. And, during reheating up to $600^{\circ}C$, grain growth of Al-Zn-Mg alloy billets contained Sc (0.1 and 0.3 mass %) was not occurred in comparison with those of Al-Zn-Mg alloy without Sc. It was thought that $Al_3Sc$ phases have a pinning effect in grain boundary and Sc content of 0.1 mass% is able to inhibit grain growth effectively through reheating process.

• 한국멀티미디어학회논문지
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• 제14권3호
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• pp.440-448
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• 2011

스마트 폰, 개인용 기기 등을 비롯한 다양한 시스템들에서 그래픽 기반의 사용자 인터페이스를 개발하기 위한 OpenGL 계열 렌더링 표준에 대한 수요가 증가하고 있다. 또한, 항공용, 군사용, 의료용, 차량용 분야의 수요를 중심으로 형성된 세이프티-크리티컬(safety-critical) 시장에서는 OpenGL의 세이프티 크리티컬 프로파일로 개발된 OpenGL SC 표준이 중요한 역할을 담당한다. 본 논문에서는 OpenGL SC 표준을 비용 대비 효과적으로 제공하기 위해서, 기존의 임베디드 시스템들에서 비교적 널리 사용되고 있는 OpenGL 1.x 파이프라인 상에서 OpenGL SC 에뮬레이션을 제공하는 방법을 제안한다. 우리가 제안하는 방법은 임베디드 시스템에서 낮은 개발비로 OpenGL SC 표준을 제공할 수 있으며, 임베디드 시스템용 PC 개발 환경에서의 에뮬레이션용을 위한 필수 요소이기도 하다. 최종 결과는 리눅스기반 시스템과 VxWORKS 기반 시스템에서 표준에 맞게 작동하고, 적합한 실행 속도를 보였다.

• 대한본초학회지
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• 제26권3호
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• pp.23-29
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• 2011

Objective : The purpose of this study was to investigate the anti-inflammatory effects of aqueous extract from Scolopendrae Corpus (SC) on lipopolysaccharide (LPS)-induced inflammatory response. Methods : To evaluate the anti-inflammatory effects of SC, we examined the inflammatory mediators such as nitric oxide (NO) and pro-inflammatory cytokines (TNF-a, inteleukin (IL)-$1{\beta}$ and IL-6) on RAW 264.7 cells. We also examined molecular mechanisms such as mitogen-activated protein kinases (MAPKs) and inhibitory kappa B a ($I{\kappa}$-Ba) using western blot. Furthermore, we also investigated the effect of SC on LPS-induced endotoxin shock. Results : Extract from SC itself had not any cytotoxic effect in RAW 264.7 cells. Aqueous extract from SC inhibited LPS-induced NO production and iNOS expression. SC pre-treatment also inhibited IL-$1{\beta}$, IL-6 production in RAW 264.7 cells. To investigate inhibitory effects of SC on inflammatory mediators, activation of MAPKs was examined. SC inhibited the phosphorylation of p38 kinases (p38), c-Jun $NH_2$-terminal kinase (JNK) and also the degradation of $I{\kappa}$-$B{\alpha}$ in RAW 264.7 cells stimulated with LPS. Furthermore, SC administration reduced LPS-induced endotoxin shock. Conclusion : SC down-regulated LPS-induced production of inflammatory mediators through inhibition of activation of p38, JNK and degradation of $I{\kappa}$-$B{\alpha}$. Taken together, our results suggest that SC may be a beneficial drug against inflammatory diseases such as sepsis.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2014년도 춘계학술대회 논문집
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• pp.540-542
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• 2014

This paper introduces main activities of ISO/TC 108/SC 3 and published standards. Two working groups (WG 1 and WG 6) have been orgnized to review and revise ISO standards, specifically related to measurement and calibration subjects of vibration and shocks. A new joint working group (JWG1) with IEC/SC 47E (Semiconductor acceleration calibration) has started to deal with calibration methods for analog and digital output MEMS accelerometers. Finally, Published ISO standards, technically super-visored by ISO/TC 108/SC 3, are listed in this paper to help readers understand their main contents.