• BMB Reports
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• v.44 no.9
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• pp.584-589
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• 2011

The mammalian ste20-like kinase (MST) pathway is important in the regulation of apoptosis and cell cycle and emerges as a novel tumor suppressor pathway. MST-induced phosphorylation of Salvador homolog 1 (SAV1), which is a scaffold protein, has not been evaluated in detail. We performed a mass spectrometric analysis of the SAV1 protein that was co-expressed with MST2. Phosphorylation was detected at Thr-26, Ser-27, Ser-36 and Ser-269. Although single or double mutations had little effects, the mutation of all four residues in SAV1 to Ala (SAV1-4A) had inhibitory effects on the MST pathway. MST2-mediated induction of SAV1-4A protein levels, SAV1-4A interaction with MST2 and the self-dimerization of SAV1-4A were weaker compared to those of wild-type SAV1. SAV1-4A inhibited MST2- and K-RasG12V-induced cell death of MCF7 cells. These results suggest that MST-mediated phosphorylation of four residues within SAV1 may be important in the induction of cell death by the MST pathway.

• Journal of the Korean Magnetic Resonance Society
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• v.16 no.2
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• pp.162-171
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• 2012

SAV2228 has an OB (Oligomer-Binding)-motif which is frequently used for nucleic acid recognition. To characterize the activity of translation initiation factor-1 (IF-1) from Staphylococcus aureus, SAV2228 was expressed and purified in Escherichia coli. We acquired 3D NMR spectra showing well dispersed and homogeneous signals which allow us to assign 94.4% of all $^1HN$, $^{15}N$, $^{13}C{\alpha}$, $^{13}C{\beta}$ and $^{13}CO$ resonances. We could predict a secondary structure of SAV2228 using TALOS and CSI from NMR data. SAV2228 was consisted of one ${\alpha}$-helix and five ${\beta}$-sheets. The predicted secondary structure, ${\beta}-{\beta}-{\beta}-{\alpha}-{\beta}-{\beta}$, was similar to other bacterial IF-1, but it was not completely same to the eukaryotic one. Assigned NMR peaks and secondary structre prediction can be used for the study on interaction with nucleic acid in the future.

• Journal of the Korean Magnetic Resonance Society
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• v.17 no.1
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• pp.54-58
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• 2013

SAV0506 is an 87 residue hypothetical protein from Staphylococcus aureus strain Mu50 and also predicted to have similar function to ribosome associated heat shock protein, Hsp 15. Hsp15 is thought to be involved in the repair mechanism of erroneously produced 50S ribosome subunit. In this report, we present the sequence specific backbone resonance assignment of SAV0506. About 82.5% of all resonances could be assigned unambiguously. By analyzing deviations of the $C{\alpha}$ and $C{\beta}$ chemical shift values, we could predict the secondary structure of SAV0506. This study is an essential step towards the structural characterization of SAV0506.

• Explosives and Blasting
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• v.25 no.1
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• pp.31-43
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• 2007

In most tunnel constructions in South Korea, blasting has been widely used as an excavation method. In tunnel blasting, the center-cut to induce first free surface is very important for enhancing excavation efficiency and reducing vibration caused by exploding. This paper introduces new center-cut method named SAV-cut (Stage Advance V-cut) developed on the concept of V-cut. Significant features of SAV-cut are the center hole and stepwise ignition. Many field tests and numerical analysis were carried out to analyze the mechanical behavior and blasting vibration. From the results, the newly developed SAV-cut was proved as an effective center-cut method for both increasing blasting efficiency and decreasing blasting vibration.

• Nuclear Engineering and Technology
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• v.53 no.10
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• pp.3398-3405
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• 2021

This study observed the effect of neutron irradiation and ageing on the microstructure, hardness, and corrosion resistance of SAV-1 (Al-Mg-Si) alloy. The investigated material was irradiated with neutrons to fluences of 10²¹-10²⁶ n/m² in the WWR-K research reactor and kept in dry storage. Long-term irradiation led to an increase in hardness of the alloy and a deterioration of pitting corrosion resistance. Post-irradiation ageing for 1 h at 100-300 ℃ resulted in a decrease in microhardness of the irradiated SAV-1. The effect of post-irradiation ageing on pitting corrosion was made clear through the formation of Guinier-Preston zones and secondary precipitates in the Al matrix. Ageing at 250 ℃ corresponded to the development of stable microstructure and the highest corrosion resistance for the irradiated samples. Mg₂Si, Si, and needle-shaped β" precipitates were formed in SAV-1 alloy that was irradiated with low fluences. β" and clusters of rod-shaped B-type precipitates were observed in highly irradiated samples. The precipitates were similar to those seen in non-irradiated pseudo-binary Al-Mg₂Si alloys with Si excess.

• Nuclear Engineering and Technology
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• v.26 no.2
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• pp.247-256
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• 1994

The nuclide chain model used in SAV90 has been modified for the CASMO3/MEDIUM3 procedure. Since the default nuclide chain in SAV90, using 21 nuclides, is not sufficient to reproduce the CASMO3 results in the MEDIUM3 calculation, the extended nuclide chain models have been investigated and verified with various types of fuel assemblies. Among the extended nuclide chain models proposed, the 22 nuclide chain model, which contains only Pu238 additionally to the 21 nuclide chain, is recommended in terms of both accuracy and computing efficiency. Using this model core follow calculations for YGN-1 have been performed. The results showed good performance when compared to plant measurements.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.55 no.3
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• pp.328-337
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• 2022

This study investigated the removal performance of a foam fractionator under seawater conditions. The foam fractionator was tested using a 3×3×3 factorial design for operating conditions by combining different solids concentrations (SS; 1, 5, and 10 mg·L^-1), surface air velocities (SAV; 1.1, 1.5, and 2.1 cm·sec^-1), and hydraulic residence times (HRT; 1, 3, and 6 min) at 16℃. Performance parameters such as daily solids removal rate and efficiency were measured, and a multi-regression model equation was developed accordingly. The daily solids removal rate and removal efficiency varied with the experimental conditions and ranged from 0.14-2.33 g-solids·m^-3-air·day^-1 and 8.9-96.7 %, respectively. Overall, the daily solids removal rate increased with increasing SS and SAV and decreasing HRT, whereas the removal efficiency increased with increasing SAV and HRT and decreasing SS. The daily solids removal rate (g-solids·m^-3-air·day^-1) of the foam fractionator for SAV (cm·sec^-1), SS (mg·L^-1) and HRT (min) were described by the following multi-regression model: Daily solids removal rate [f(z)]=-0.118+0.422SAV+0.094HRT+0.141SS (r²=0.873).

• Korean Journal of Fisheries and Aquatic Sciences
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• v.36 no.4
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• pp.340-345
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• 2003

In order to choose the manufacturing method for extracting alginates from sea tangle, Laminaria japonica, three methods were applied. In Method I, alginates were extracted with NaOH solution from sea tangle powder and extracted alginates were precipitated and converted to alginic acid by $CaCl_2$ and HCI solution. Then alginic acid was converted to sodium alginates with $Na_2CO_3$ solution. Sodium alginates were precipitated with methyl alcohol and were resolved with hot water and this step was repeated three times. Method II was same to Method I except final step including that sodium alginates were precipitated and washed with methyl alcohol three times. Method III included that sodium alginates were extracted with $Na_2CO_3$ solution from sea tangle powder then sodium alginates were precipitated and washed with methyl alcohol three time. Extracting time increased with Increasing extracted alginates amounts but increasing rates were below $0.4\%/h.$ Alginates amounts recovered by Method III showed above 2 times more to those by Method I and II. Extracting time increased with increasing ash amount of sodium alginates but increasing rates were below $0.1\%/h.$ and that of sodium alginates extracted by Method III showed higher value $(5\%)$ than those by Method I and II. In the sodium alginates prepared by Method III, the amount of ash in alginates dried by air was $34.4\%,$ that by vacuum freeze drying was $47.8\%.$ Extracting time increased with decreasing average molecular weight (MW) and degree of polymerization (DP) of sodium alginates, MW and DP of alginates prepared by Method III were higher than those by Method I and II In same extracting time. Extracting time increased with decreasing rate of apparent viscosity change (SAV) of alginates solution, and SAV of alginates prepared by Method III showed higher value than those by Method I and II in same extracting time. SAV of alginates dried by air was higher than that by vacuum freeze drying. Relating equation among SAV MW and DP were MW = 60.066 (SAV) -93.950, DP =309.760 (SAV) -485.084 and MW = 0.914 (DP)+0.213.

• Korean Journal of Plant Taxonomy
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• v.39 no.3
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• pp.130-134
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• 2009

A new species of Apiaceae, Sium ternifolium from Mt. Chiak-san National Park, Gangwon-do province, Korea, is described and illustrated. This new species is closely related to S. serra (Fr. & Sav.) Kitag., having such characters as long acuminated apex of leaflets, 3-6 slender rays, but distinguished from the latter by its lower height, the absence of involucral bracts, and tri-foliated leaves.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.37 no.1
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• pp.1-6
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• 2004

In preparing sodium alginates from sea tangle (Laminaria japonica) powder using the Mexican process, alkaline extraction, conversion to alginic acid and reversion to sodium alginates were used to increase purity. The effect of hot water treatment and dialysis on measuring the average molecular weight of sodium alginates were investigated. Intrinsic viscosity and average molecular weight of sodium alginates after dialysis were higher than those before dialysis. Average molecular weight of sodium alginates treated with hot water was higher than that without. Hot treatment has little effect on the ash content of sodium alginates. Ash content of sodium alginates before dialysis were $27-30\%$ those after dialysis were $10\%.$ After dialysis, Na content was highest $(89-91\%),$ K was $11-12\%,$ Ca was $1.9\%,$ and Mg was $0.05\%.$ Ash content of alginates had little effect on average molecular weight. SAV (slope of apparent viscosity) of alginates solution after dialysis showed higher values than before. SAV of the alginates with hot water treatment were higher than without treatment.