Browse > Article
http://dx.doi.org/10.5657/kfas.2003.36.4.340

Effects of Extracting and Drying Method on Physical Properties of Alginates from Sea Tangle, Laminaria japonica  

YOU Byeong Jin (Department of Food Science, Kangnung National University)
LIM Yeong Seon (East coastal Marine Bioresources Research Center (EMBRC), Kangnung National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.36, no.4, 2003 , pp. 340-345 More about this Journal
Abstract
In order to choose the manufacturing method for extracting alginates from sea tangle, Laminaria japonica, three methods were applied. In Method I, alginates were extracted with NaOH solution from sea tangle powder and extracted alginates were precipitated and converted to alginic acid by $CaCl_2$ and HCI solution. Then alginic acid was converted to sodium alginates with $Na_2CO_3$ solution. Sodium alginates were precipitated with methyl alcohol and were resolved with hot water and this step was repeated three times. Method II was same to Method I except final step including that sodium alginates were precipitated and washed with methyl alcohol three times. Method III included that sodium alginates were extracted with $Na_2CO_3$ solution from sea tangle powder then sodium alginates were precipitated and washed with methyl alcohol three time. Extracting time increased with Increasing extracted alginates amounts but increasing rates were below $0.4\%/h.$ Alginates amounts recovered by Method III showed above 2 times more to those by Method I and II. Extracting time increased with increasing ash amount of sodium alginates but increasing rates were below $0.1\%/h.$ and that of sodium alginates extracted by Method III showed higher value $(5\%)$ than those by Method I and II. In the sodium alginates prepared by Method III, the amount of ash in alginates dried by air was $34.4\%,$ that by vacuum freeze drying was $47.8\%.$ Extracting time increased with decreasing average molecular weight (MW) and degree of polymerization (DP) of sodium alginates, MW and DP of alginates prepared by Method III were higher than those by Method I and II In same extracting time. Extracting time increased with decreasing rate of apparent viscosity change (SAV) of alginates solution, and SAV of alginates prepared by Method III showed higher value than those by Method I and II in same extracting time. SAV of alginates dried by air was higher than that by vacuum freeze drying. Relating equation among SAV MW and DP were MW = 60.066 (SAV) -93.950, DP =309.760 (SAV) -485.084 and MW = 0.914 (DP)+0.213.
Keywords
Laminaria japonica; Alginates; Average molecular weight (MW); Viscosity; Degree of polymerization (DP);
Citations & Related Records
연도 인용수 순위
  • Reference
1 Hirst, E.L., E. Percival and J.K. Wold. 1964. The structure of alginic acid. Part IV. Partial hydrolysis of the reduced polysaccharide. J. Chem. Soc., 8, 1493-1499
2 Kennedy, M., P.G. Burstyn and D.R. husbands. 1978. Fat induced hypertension in rabbits. 2. The effects of feeding diets containing high concentrations of safflower oil & palm oil. Proc. Nutr. Soc., 37, 98A
3 Kimmura, T., K. Takahashi, Y. Ueda, H. Obika, Y. Kobayashi and K. Tsuji. 1993. Effects of the primary structure of alginate on fecal excretion of sodium in rats. Nippon Nogeikagaku Kaishi, 67, 1177-1183   DOI   ScienceOn
4 Armstrong, B., A.J. Van Merwyk and H. Coates. 1977. Blood pressure in seventh-day advantist vegeterians. Am. J. Epidemiol., 105, 444-449   DOI
5 Brussard, J.H., J.M.A. Van Raaij, M. Stasse-Wolthuis, M.B. Katan and J.G.A.J. Hautvast. 1981. Blood pressure and diet in normotensive volunteers: Absence of an effect of dietary fiber, protein, or fat. Am. J. Clin. Nutr., 34, 2023-2029
6 Chapman, D.J. 1980. Algin and alginates. In: Seaweeds and Their Uses, Chapman, V.J. ed. Chapman and Hall, New York, pp. 194-225
7 Dubois, M., K.A. Gilles, J.K. Hamilton, P.A. Rebers and F. Smith. 1956. Colarimetric method for determination of sugars and related substances. Anal. Chem., 28, 350-357   DOI
8 Ducan, D.B. 1955. Multiple-range and multiple F tests. Biometics, 11, 1-42   DOI   ScienceOn
9 Fujihara, M. and T. Nagumo. 1993. An influence of the structure of alginate on the chemotectic activity of macrophages and the antitumor activity. Carbohydr.Res., 243, 211-215   DOI   ScienceOn
10 Fujiki, K., H. Matsuyama and T. Yano. 1994. Protective effect of sodium alginates against bacterial infection in common carp, Cyprinus Carpio. L. J. Fish Dis., 17, 349-354   DOI   ScienceOn
11 Gardey, T., P.G. Burstyn and T.G. Taylor. 1978. Fat induced hypertention in rabbits. I. The effects of fibre on the blood pressure increase induced by coconut oil. Proc. Nutr. Soc., 37, 97A
12 Hajime, O., S. Yasushi, Y. Kanto, U. Isamu and K. Koichi. 1994. Possible antitumor promoting properties of marine algae and in vitro activity of Wakame seaweed extract. Biosci. Biotech. Biochem., 56, 994-999   DOI   ScienceOn
13 Penman, A. and G.R. Sanderson. 1972. A method for the determination of uronic acid sequence in alginate. Carbohydr. Res., 25, 273-282   DOI   ScienceOn
14 AOAC. 1990. Official Methods of Analysis, 15th ed. Association of Official Analytical Chemists. Arling-ton, pp. 17
15 Wright, A., P.G. Burstyn and M.J. Gibney. 1979. Dietary fibre and blood pressure. Br. Med. J., 2, 1541-1543   DOI
16 You, B.J., Y.S. Im, I.H. Jeong and K.H. Lee. 1997. Effect of extraction conditions on bile acids binding capacity in vitro of alginate extracted from sea tangle (Laminaria spp.). J. Kor. Fish. Soc., 30, 31-38 (in Korean)
17 Kobayashi, N., Y. Kanazawa, S. Yamabe, K. Iwata, M. Nishizawa, T. Yamagishi, O. Nishikaze and K. Tsuji. 1997. Effects of depolymerized sodium alginate on serum total cholesterol in healthy women with a high cholesterol intake. J. Home Econo. Japan, 48, 255-230
18 Mancini, M., M. Moresi and F. Sappino. 1996. Rheological behaviour of aqueous dispersions of algal sodium alginates. J. Food Eng., 28, 283-295   DOI   ScienceOn
19 Mitchell, J.R. and D.A. Ledward. 1986. Functional Pro-perties of Food Macromolecules. Elsvier Applied Science Pub., London, pp. 1-78
20 Oerther, S., H. Le Gall, E. Payan, F. Lapicque, N. Presle, P. Hubert, J. Dexheimer, P. Netter and F. Lapicque. 1999. Hyaluronate-alginate gel as a novel biomaterial: Mechanical properties and formation mechanism. Biotech. Bioengin., 63, 206-215   DOI   ScienceOn
21 Somogyi, M. and N. Nelson. 1952. Notes on sugar determi-nation. J. BioI. Chem., 195, 19-23
22 Harrison, G.E., E.R. Humphreys, A. Sutton and H. Shephard. 1966. Strontium uptake in rats on alginate-supplemented diet. Science, 152, 655-656   DOI   ScienceOn
23 SPSS, Inc. 2001. Sigma Plot 7.0 for window, SPSS. Inc., 233 South Wacker Drive, Chicago, USA, 11 60606-6307
24 Suzuki, T., K. Nakai, Y. Yoshie, T. Shirai and T. Hirano. 1993. Digestibility of dietary fiber in brown alga, kombu, by rats. Nippon Suisan Gakkaishi, 59, 879-884   DOI
25 Turquois, T. and H. Gloria. 2000. Determination of the absolute molecular weight averages and molecular weight distributions of alginates used as ice cream stabilizers by using multiangle laser light scattering measurements. J. Agric. Food Chem., 48, 5455-5458   DOI   ScienceOn
26 Haug, A. 1959. Ion exchange properties of alginate fractions. Acta Chem. Scand., 13, 1250-1251   DOI
27 Haug, A. 1961. The affinity of some divalent metals to different types of alginates. Acta Chem. Scand., 15, 1794-1795   DOI
28 Haug, A. 1964. Composition and properties of alginates. Rept. 30, Norwegian Institute Seaweed Reserch, Trondheim, Norway
29 Haug, A. and B. Larsen. 1962. Quantitative determination of the uronic acid composition of alginates. Acta Chem. Scand., 16, 1908-1918   DOI
30 Hedeki, O., S. Jitsuo and K. Yoshinari. 1993. Direct control of the constituents ratio in a wide range in alginate produced by Azobacter vinelandii. Biosci. Biotech. Biochem., 57, 332-336   DOI
31 Hidaka, H., T. Eida, T. Takizawa, T. Tokuzawa and Y. Tashiro. 1986. Effect of fructooligosaccharide on intestinal flora and human health. Bifido. Microbiol.,5, 37-50