• Title/Summary/Keyword: SAM

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S-Adenosylmethionine (SAM) Regulates Antibiotic Biosynthesis in Streptomyces spp. in a Mode Independent of Its Role as a Methyl Donor

  • Zhao Xin-Qing;Jin Ying-Yu;Kwon Hyung-Jin;Yang Young-Yell;Suh Joo-Won
    • Journal of Microbiology and Biotechnology
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    • v.16 no.6
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    • pp.927-932
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    • 2006
  • S-Adenosylmethionine (SAM) is a ubiquitous biomolecule serving mainly as a methyl donor. Our recent studies revealed that SAM controls antibiotic production in Streptomyces. In this study, the functional mode of SAM was studied in S. coelicolor and S. antibioticus ATCC11891, employing S-adenosylhomocysteine (SAH), a methylation reaction product of SAM. Actinorhodin biosynthesis did not require SAM as a methyl donor, whereas SAH enhanced the actinorhodin biosynthesis up to the level comparable to SAM, and the most effective concentration of SAH was higher than that of SAM. In the case of oleandomycin that requires SAM for its biosynthesis, both SAM and SAH at the concentration as low as 100 mM showed comparable efficacy in enhancing the production; SAM at 1 mM concentration additionally stimulated to give a 5-fold enhancement of oleandomycin production. In vitro autophosphorylation of protein kinase AfsK was found to be activated by both SAM and SAH, as well as other structurally related compounds. Our studies demonstrate that SAM regulates antibiotic biosynthesis in a mode independent of its role as a methyl donor and suggest that SAM acts directly as an intracellular signaling molecule for Streptomyces.

Hydrophobic Self-assembled Monolayer(SAM) Coating for Enhanced Demolding Performance in Micromolding (마이크로몰딩의 이형성 향상을 위한 소수성 Self-assembled Monolayer(SAM) 코팅)

  • Park, Sang-Ha;Han, Seung-O;Park, Jong-Yeon;Mun, Seong-Uk;Park, Jeong-Ho
    • The Transactions of the Korean Institute of Electrical Engineers C
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    • v.51 no.4
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    • pp.175-183
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    • 2002
  • In this paper, the surface modification effect of self-assembled monolayer(SAM) of 1-dodecanethiol [$CH_3$($CH_2$)$_{11}$SH] used as an anti-adhesive film in micromolding process was studied. Monolayers of 1-dodecanethiol[$CH_3$(CH$_2$)$_{11}$SH] were obtained by immersing a metal place in pure 1-dodecanethiol. SAM film on the nickel plate has been examined by using X-ray photoelectron spectroscopy(XPS). The focus has been placed on S-Ni bonding. From the XPS analysis, sulfur atoms were detected from the SAM film as a chemical composition of S-Ni. In order to measure an adhesion force of the SAM-coated nickel surface, atomic force microscopy(AFM) was used in force-distance mode, which whows the micro-adhesive force on solid surface. It was shown that adhesion forces measured from the SAM-coated nickel surface and the Ni surface without SAM coating were 3.52nN and 5.32nN, respectively. In order to investigate the effect of SAM coating on the surface foughness the replica in demolding process, hot embossing experiments were performed using a SAM-coated nickel master and a nickel master without SAM coating. Surface roughness of replica from the SAM-coated master showed 25nm and that of replica from master without SAM coating was 35nm. The smoother surface roughness of the replica from the SAM-coated, master is believed to result from reduction in the adhesion forces.ces.

Application of Geo-Segment Anything Model (SAM) Scheme to Water Body Segmentation: An Experiment Study Using CAS500-1 Images (수체 추출을 위한 Geo-SAM 기법의 응용: 국토위성영상 적용 실험)

  • Hayoung Lee;Kwangseob Kim;Kiwon Lee
    • Korean Journal of Remote Sensing
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    • v.40 no.4
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    • pp.343-350
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    • 2024
  • Since the release of Meta's Segment Anything Model (SAM), a large-scale vision transformer generation model with rapid image segmentation capabilities, several studies have been conducted to apply this technology in various fields. In this study, we aimed to investigate the applicability of SAM for water bodies detection and extraction using the QGIS Geo-SAM plugin, which enables the use of SAM with satellite imagery. The experimental data consisted of Compact Advanced Satellite 500 (CAS500)-1 images. The results obtained by applying SAM to these data were compared with manually digitized water objects, Open Street Map (OSM), and water body data from the National Geographic Information Institute (NGII)-based hydrological digital map. The mean Intersection over Union (mIoU) calculated for all features extracted using SAM and these three-comparison data were 0.7490, 0.5905, and 0.4921, respectively. For features commonly appeared or extracted in all datasets, the results were 0.9189, 0.8779, and 0.7715, respectively. Based on analysis of the spatial consistency between SAM results and other comparison data, SAM showed limitations in detecting small-scale or poorly defined streams but provided meaningful segmentation results for water body classification.

The Research Regarding Cheong-Sam Pattern of Fabric Design in the Period of the Republic of China(1912-1949) - Comparison Between Jing Pai and Hai Pai - (중국 민국시대(1912년-1949년)에 나타난 치파오 문양에 관한 연구 - 경파이 치파오와 해파이 치파오의 문양 비교를 중심으로 -)

  • Seo, A-Jeong;Oh, Hee-Kyoung;Kim, Sook-Jin
    • Journal of the Korea Fashion and Costume Design Association
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    • v.15 no.3
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    • pp.69-83
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    • 2013
  • Clothes show not just the different social status of people, but the ideology and value of former society through pattern, colour, materials, shapes, etc. The purpose of this article is to fill the academic blank of this part by researching the pattern of fabric design in Jing Pai(Beiing style) and Hai Pai(Shanghai style) cheong-sam during the period of the Republic of China. The contrastive analysis of regional pattern between Jing Pai and Hai Pai cheong-sam expect to provide the theoretical basis for the former fashion designers and scholars. There are three approaches in the article: Data collection method, comparison method and Combining theory with practice method as film. Regarding components of pattern, both Jing Pai and Hai Pai cheong-sam have mostly single or composite pattern like plants. Further the most of Jing Pai cheong-sam pattern is traditional flower pattern. But Haipai cheong-sam patterns have some western flower pattern. Beside that, it have some geometry pattern. Regarding arrangement of the pattern, both cheong-sams have scattered dot style, the border style, and pictures style. But continuous type of Jing Pai cheong-sam is less while Hai Pai cheong-sam is the most. Comparing Jing Pai cheong-sam color of patterns in "Moment in Peking" is unadorned and types are simple as chinese traditional clothes; However, "In the Mood for Love" introduces us various material colors, new types of patterns and extraneous characteristic geometry patterns of Hai Pai cheong-sam. Generally speaking, the main characteristic of Jing Pai cheong-sam is traditional and conservatism. It keeps Chinese traditional pattern and culture to the most extent. However, Hai Pai cheong-sam are confluent and open with absorbed external culture and techniques which are endowed new artistic color.

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ITO 표면의 자기 조립 단일막 형성에 의한 유기 발광 소자의 특성

  • Na, Su-Hwan;Mok, Rang-Gyun;Kim, Tae-Wan;Hong, Jin-Ung;Jeong, Dong-Hoe
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2009.11a
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    • pp.168-169
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    • 2009
  • 본 연구에서는 ITO 표면 개질에 의한 유기 발광 소자의 특성 변화에 대해서 연구하였다. ITO 전극은 발광 소자의 투명 전극으로 널리 사용되고 있으며 이러한 발광 소자의 특성은 ITO의 표면 상태에 따라 민감하게 반응한다. ITO 표면 개질은 ITO와 유기물 사이의 쇼트기 장벽을 감소시키며, 전극과 유기물의 점착을 향상시켜 준다. 본 실험에서는 습식 처리 방식으로 self-assembled monolayer(SAM)을 사용하였다. 유기 발광 소자의 특성은 SAM 처리에 의해 향상 되었다. 유기 발광 소자는 ITO/SAM/TPD(50nm)/$Alq_3$(70nm)/LiF(0.5nm)/Al(100nm)의 구조로 제작하였으며, ITO의 표면 특성은 일반적인 특성 기술에 의해 연구되었다. SAM 처리된 소자는 SAM 처리하지 않은 소자에 비해 구동 전압, 발광 세기, 외부 양자 효율 등이 향상되었다. ITO의 SAM 처리 시간을 0/10/15/20/25분으로 하여 소자를 제작하였다. 15분간 SAM 처리한 소자는 SAM 처리하지 않은 소자에 비해 외부 양자 효율과 전류 효율이 2.6배 상승하였다. 본 실험을 통하여 ITO 표면 위에 SAM층을 삽입한 걸과, 구동 전압, 발광 세기, 효율 등이 향상됨을 알 수 있었다.

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Selection and Characteristics of Fermented Salted Seafood (jeotgal)-Originated Strains with Excellent S-adenosyl-L-methionine (SAM) Production and Probiotics Efficacy

  • Kim, Min-Jeong;Park, Sunhyun;Lee, Ran-Sook;Lim, Sang-Dong;Kim, Hyo Jin;Lee, Myung-Ki
    • Food Science of Animal Resources
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    • v.34 no.1
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    • pp.65-72
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    • 2014
  • This study is executed to develop probiotics which produce S-adenosyl-L-methionine (SAM), a methyl group donor of the 5-methyltetrahydrofolate methylation reaction within the animal cell. SAM is an essential substance for the synthesis, activation, and metabolism of hormones, neurotransmitters, nucleic acids, phospholipids, and cell membranes of animals. The SAM is also known as a nutritional supplement to improve brain functions of the human. In this study, the SAM-producing strains are identified in 18 types of salted fish, and then, the strains with excellent SAM productions are being identified, with 1 strain in the Enterococcus genus and 9 strains in the Bacillus genus. Strains with a large amount of SAM production include the lactic acid bacteria such as En. faecium and En. durans, En. sanguinicola, as well as various strains in the Bacillus genus. The SAM-overproducing strains show antibacterial activities with certain harmful microbes in addition to the weak acid resistances and strong bile resistances, indicating characteristics of probiotics. It is possible that the jeotgal-originated beneficial strains with overproducing SAM can be commercially utilized in order to manufacture SAM enriched foods.

Evaluation of S-Adenosyl-L-Methionine Production by Bifidobacterium bifidum BGN4

  • Kim, Ji-Youn;Suh, Joo-Won;Ji, Geun-Eog
    • Food Science and Biotechnology
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    • v.17 no.1
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    • pp.184-187
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    • 2008
  • S-Adenosyl-L-methionine (SAM) is an important metabolic intermediate in living organisms and participates in many reactions as a methyl group donor. SAM has been used as a dietary supplement and is proposed to have beneficial effects on the liver and brain. The aim of this study was to find lactic acid bacteria with high SAM-producing ability to be used as SAM enhancing probiotics. We used high performance liquid chromatography (HPLC) to quantify the amount of SAM produced, and found that Bifidobacterium bifidum BGN4 produced a significantly higher amount of SAM than other Bifidobacterium or Lactobacillus strains. The effect of various carbon and nitrogen sources on SAM production was examined. This study confirmed that Bifidobacterium may be utilized as a source of SAM in the functional food industry.

Tribological Characteristics of FDTS & OTS SAM according to Annealing Temperature (FDTS와 OTS SAM의 어닐링 온도에 따른 트라이볼로지 특성)

  • 양지철;김대은
    • Journal of the Korean Society for Precision Engineering
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    • v.20 no.1
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    • pp.240-247
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    • 2003
  • The tribological characteristics of FDTS (1H, 1H, 2H, 2H-Perflurodecyltrichlorosilane) SAM (self-assembled monolayer) and OTS (octadecyltrichlorosilane) SAM treated by high temperature annealing have been investigated from the viewpoint of stiction, adhesion and friction in micro/nano scale. From the experimental results, it was found that OTS SAM gets destroyed at 20$0^{\circ}C$ and stiction, adhesion and friction coefficient increased, but FDTS SAM was stable up to 40$0^{\circ}C$. Also, it was found that the friction coefficient of normal OTS SAM below 20$0^{\circ}C$ is lower than that of FDTS SAM in micro/nano scale, but stiction and adhesion is vice versa. This work shows the importance of surface group of self-assembled monolayer in dictating the tribological characteristics and thermal stability.

Commercial MgB2 superconducting wires at Sam Dong

  • Lee, Dong Gun;Choi, Jun Hyuk;Kim, Du Na;Jeon, Ju Heum;Maeda, Minoru;Choi, Seyong;Kim, Jung Ho
    • Progress in Superconductivity and Cryogenics
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    • v.22 no.2
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    • pp.26-31
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    • 2020
  • Since 2014, Sam Dong Co., Ltd. has successfully developed high-performance MgB2 superconducting wires with a kilometer-scale. Herein, we studied performances of various MgB2 wires fabricated by the Sam Dong with different Cu fractions and diameters for practical applications. Critical current densities of our commercial wire, 18+'1'Cu multifilamentary MgB2 wire, are estimated to be 270,000 A/㎠ at 3 T and 4.2 K and 100,000 A/㎠ at 2 T and 20 K, respectively. We further discuss research progress of various MgB2 superconducting wires at Sam Dong Co., Ltd and make an effort to align with customers' requirements.

The Optimal Allocation Model for SAM Using Multi-Heuristic Algorithm : Focused on Theater Ballistic Missile Defense (복합-휴리스틱 알고리즘을 이용한 지대공 유도무기(SAM) 최적배치 방안 : 탄도미사일 방어를 중심으로)

  • Lee, Jae-Yeong;Kwak, Ki-Hoon
    • IE interfaces
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    • v.21 no.3
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    • pp.262-273
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    • 2008
  • In Korean peninsular, Air Defense with SAM(Surface-to-Air Missile) is very important, because of threatening by North Korea's theater ballistic missiles installed with nuclear or biochemistry. Effective and successful defense operation largely depends on two factors, SAM's location and the number of SAM for each target based on missile's availability in each SAM's location. However, most previous papers have handled only the former. In this paper, we developed Multi-heuristic algorithm which can handle both factors simultaneously for solving allocation problem of the batteries and missile assignment problem in each battery. To solve allocation problem, genetic algorithm is used to decide location of the batteries. To solve missile assignment problem, a heuristic algorithm is applied to determine the number of SAM for each target. If the proposed model is applied to allocation of SAM, it will improve the effectiveness of missile defense operations.