• 토지주택연구
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• 제6권1호
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• pp.21-29
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• 2015

연결보는 지진하중에 효과적으로 저항하기 위하여 적절한 강도, 강성, 변형능력을 지녀야 한다. 특히 스팬-춤 비가 2.0 이하인 대각선다발철근을 갖는 특수전단벽 연결보는 일반 연결보보다 더 높은 강도, 강성, 연성능력을 갖게 되나 대각선다발철근 상세는 시공에 큰 어려움이 발생한다. 본 연구에서는 이러한 문제에 대한 해결방안의 하나로서 대각선다발철근 상세를 대체하기 위한 대안상세들이 실험적으로 연구되었다. 실험결과, 앵글형태로 보강된 SA실험체가 대각방향 보강근을 완전히 제거한 SB시리즈의 실험체와 비교하여 더 안정된 거동을 보였으며, 기존의 대각선다발철근상세를 갖는 CA실험체와 비교하여 유사한 강도, 강성, 에너지소산능력과 변형능력(drift)을 나타내었다.

• 자원리싸이클링
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• 제20권3호
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• pp.55-61
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• 2011

금속급 실리콘(MG-Si)을 태양전지용 실리콘(SOG-Si)으로 정제하기 위한 경제적인 프로세스를 구축하기 위하여 1823 K에서 CaO-$SiO_2$ 계 슬래그에 의한 붕소의 제거에 대하여 조사하였다. 본 연구에서 CaO-$SiO_2$와 $CaCO_3-SiO_2$ 슬래그의 염기도(%CaO/$%SiO_2$) 증가에 따라 B의 제거율은 각각 63%와 73%까지 증가하였다. 그러나 Ar 가스에 의한 슬래그와 실리콘의 교반 시간의 영향은 나타나지 않았다. 그리고 CaO-$SiO_2$ 계 슬래그에 $Na_2CO_3$를 첨가하였으나 그 영향은 크지 않았다. $CaCO_3-SiO_2$ 슬래그(염기도=1.2)에 의해 3회 처리한 결과 B의 농도는 1.03 ppm까지 감소하였다.

• Journal of Microbiology and Biotechnology
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• 제20권9호
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• pp.1359-1366
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• 2010

A search of the Streptomyces avermitilis genome reveals that its closest homologs are several O-methyltransferases. Among them, one gene (viz., saomt5) was cloned into the pET-15b expression vector by polymerase chain reaction using sequence-specific oligonucleotide primers. Biochemical characterization with the recombinant protein showed that SaOMT5 was S-adenosyl-L-methionine-dependent Omethyltransferase. Several compounds were tested as substrates of SaOMT5. As a result, SaOMT5 catalyzed O-methylation of flavonoids such as 6,7-dihydroxyflavone, 2',3'-dihydroxyflavone, 3',4'-dihydroxyflavone, quercetin, and 7,8-dihydroxyflavone, and phenolic compounds such as caffeic acid and caffeoyl Co-A. These reaction products were analyzed by TLC, HPLC, LC/MS, and NMR spectroscopy. In addition, SaOMT5 could convert phenolic compounds containing ortho-dihydroxy groups into O-methylated compounds, and 6,7-dihydroxyflavone was known to be the best substrate. SaOMT5 converted 6,7-dihydroxyflavone into 6-hydroxy-7-methoxyflavone and 7-hydroxy-6-methoxyflavone, and caffeic acid into ferulic acid and isoferulic acid, respectively. Moreover, SaOMT5 turned out to be a $Mg^{2+}$-dependent OMT, and the effect of $Mg^{2+}$ ion on its activity was five times greater than those of $Ca^{2+}$, $Fe^{2+}$, and $Cu^{2+}$ ions, EDTA, and metal-free medium.

• Journal of the Korean Wood Science and Technology
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• 제38권3호
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• pp.223-229
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• 2010

본 연구에서는 천연리그닌의 전구물질인 3종의 모노리그놀[p-coumaryl alcohol (PCA), coniferyl alcohol(CA), sinapyl alcohol (SA)]을 이용하여 horseradish peroxidase (HRP, EC. 1.11.1.7)/$H_2O_2$ 조건하에서 dehydrogenative polymers (DHPs)를 제조하였다. 합성한 DHPs와 천연리그닌의 구조적 특성을 비교하기 위해 소나무와 포플라 milled wood lignin (MWL)과 함께 Gel permeation chromatography (GPC)에 의한 분자량 측정과 Derivatization followed by reductive cleavage (DFRC) 분석에 의한 ${\beta}$-O-4 결합 빈도를 측정하였다. DHP 합성 수율은 CA만을 단독으로 주입한 G-DHP가 71%로 가장 높았고 PCA에 의한 H-DHP 수율은 42%로 나타났다. 그러나 horseradish peroxidase/$H_2O_2$ 조건하에서 SA 단독으로는 S-DHP는 전혀 합성되지 않았다. 합성한 DHPs의 분자량(Mw)은 3,000~4,700 범위로 측정되었는데, 이는 침엽수 리그닌인 소나무 MWL (G-type lignin: Mw 7340)의 절반 정도였고 활엽수인 포플라 MWL (GS-type lignin: Mw 13,250)의 1/3 수준으로 측정되었다. DHP 합성 과정에서 형성된 ${\beta}$-O-4 결합 빈도는 GS-DHP가 502 ${\mu}mol$/g으로 가장 높았지만 포플라 MWL (1107 ${\mu}mol$/g)의 1/2 수준에 머물렀고, G-DHP의 경우도 약 286 ${\mu}mol$/g으로 H-DHP (127 ${\mu}mol$/g)보다 약 2.5배 이상 많은 ${\beta}$-O-4 결합을 형성하였지만 소나무 MWL (651 ${\mu}mol$/g)과 비교하여 절반 정도로 측정되었다.

• 대한전기학회논문지:전기물성ㆍ응용부문C
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• 제50권10호
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• pp.504-508
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• 2001

The$(Sr_{1-x}Ca_x)TiO_3$(SCT) thin films are deposited on Pt-coated electrode $(Pt-TiN /SiO_2Si)$ using RF sputtering method at various deposition temperature. The crystallinity of thin films was increased with increased of deposition temperature n the temperature range of 200~500 $[^{\circ}C]$. The capacitance changes almost linearly in temperature ranges of -80~+90$[^{\circ}C]$. All SCT thin films used in the study the phenomena of dielectric relaxation with the increase of frequency, and the relaxation frequency is observed above 200[kHz]. V-I characteristics of SCT thin films show the increasing leakage current with the increases of deposition temperature.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 2003년도 정기총회 및 학술발표회
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• pp.50-50
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• 2003

We have identified and analysed a putative response regulator two-component gene (CaSKN7) from Candida albicans and its encoding protein (CaSkn7). CaSKN7 has an open reading frame of 1677bp. CaSKN7 encodes a 559 amino acid protein (CaSkn7) with an estimated molecular mass of 61.1 kDa. CaSKN7 is a homologue of a Saccharomyces cerevisiae SKN7 that is the regulator involved in the oxidative stress response. To study the role of CaSKN7, we constructed a CAI4-derived mutant strain carrying a homozygous deletion of the CaSKN7 gene. In the caskn7 disruptant cells, the formation of germ tube require shorter time than that in the congenic wild-type strain but the growth of mycelium delayed in liquid media. In contrast, the caskn7 disruptant cells attenuate the differentiation in solid media and the virulence in mouse model system. Expression level of hypha-specific and virulence genes - HYR1, ECE1, HWP1, and ALS1 - in the caskn7 disruptant cells increased as compared with that in the congenic wild-type strain in 10％ serum YPD. Skn7 in 5. cerevisiae was found to bind the HSE element from the SSA promoter, Also, CaSkn7 contains heat shock factor DNA-binding domain and the promoters of these genes have HSE-like sties. Therefore these results show that CaSKN7 regulate the differentiation and virulence of C. albicans.

• The Plant Pathology Journal
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• 제18권4호
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• pp.192-198
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• 2002

Mixed infections of two economically important viruses, Turnip mosaic virus(TuMV) in the family Potyviridae and Ribgrass mosaic virus(RMV) in the genus Tobamo-virus, were studied ultrastructurally on oriental cabbage. TuMV-ACl8 (alpine isolate in Korea) induced chlorotic spots on inoculated leaves of both ‘SSD63’ inbred line known as susceptible to TuMV, and ‘Tambok’ commercial cultivar, known as resistant to the virus, in the early stages of infection. TuMV-C5 (Taiwan isolate) caused severe mosaic and malformation on the upper leaves of ‘SSD63’, and necrotic spots in both inoculated and upper leaves of ‘Tambok’. RMV-CA1 (oriental cabbage isolate from alpine in Korea) induced vein chlorosis, leaf malformation, and midrib necrotic streak in the upper leaves of both ‘SSD63’ and ‘Tambok’. Both oriental cabbages infected with a combination of TuMV-ACl8 and RMV-CA1 showed synergistic symptoms of severe yellowing, severe mosaic, and necrotic spot or vein necrosis on their leaves. A combination of TuMV-C5 and RMV-CA1 produced synergistic symptoms only in ‘SSD63’. In ‘Tambok’ infected with the combination of TuMV-C5 and RMV-CA1, the number of necrotic spots on the inoculated leaves was one half lesser than that on singly infected with TuMV-C5. A few necrotic spots progressed systemically. In cells infected with a combination of TuMV-ACl8 and RMV-CA1 or TuMV-C5 and RMV-CA1, the particles of the two viruses made nonagon-like rings(NLR); one TuMV particle was surrounded loosely by nine RMV particles. Two unrelated viruses of TuMV and RMV were compacted in the central part of the spiral aggregates(SA) that was induced strikingly in cells by the mixed infections. The SA showed NLR in its center of the cross-sectioned side. Many particles of RMV of Tobamovirus were closely associated with Potyvirus-characteristic cylindrical inclusions. The SAs in the mixed infections were formed easily by the Potyvirus of TuMV-ACl8 or -C5 isolates.

• BMB Reports
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• 제55권12호
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• pp.627-632
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• 2022

Dickkopf-1 (DKK1) is a secreted protein that acts as an antagonist of the canonical WNT/β-catenin pathway, which regulates osteoblast differentiation. However, the role of DKK1 on osteoblast differentiation has not yet been fully clarified. Here, we investigate the functional role of DKK1 on osteoblast differentiation. Primary osteoprogenitor cells were isolated from human spinal bone tissues. To examine the role of DKK1 in osteoblast differentiation, we manipulated the expression of DKK1, and the cells were differentiated into mature osteoblasts. DKK1 overexpression in osteoprogenitor cells promoted matrix mineralization of osteoblast differentiation but did not promote matrix maturation. DKK1 increased Ca⁺ influx and activation of the Ca⁺/calmodulin-dependent protein kinase II Alpha (CAMK2A)-cAMP response element-binding protein 1 (CREB1) and increased translocation of p-CREB1 into the nucleus. In contrast, stable DKK1 knockdown in human osteosarcoma cell line SaOS2 exhibited reduced nuclear translocation of p-CREB1 and matrix mineralization. Overall, we suggest that manipulating DKK1 regulates the matrix mineralization of osteoblasts by Ca⁺-CAMK2A-CREB1, and DKK1 is a crucial gene for bone mineralization of osteoblasts.

• Journal of Periodontal and Implant Science
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• 제49권6호
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• pp.366-381
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• 2019

Purpose: The purpose of this study was to evaluate the effectiveness of conventional sandblasted, large-grit, acid-etched (SLA) surface coated with a pH buffering solution based on surface wettability, blood protein adhesion, osteoblast affinity, and platelet adhesion and activation. Methods: Titanium discs and implants with conventional SLA surface (SA), SLA surface in an aqueous calcium chloride solution (CA), and SLA surface with a pH buffering agent (SOI) were prepared. The wetting velocity was measured by the number of threads wetted by blood over an interval of time. Serum albumin adsorption was tested using the bicinchoninic acid assay and by measuring fluorescence intensity. Osteoblast activity assays (osteoblast adhesion, proliferation, differentiation, mineralization, and migration) were also performed, and platelet adhesion and activation assays were conducted. Results: In both the wetting velocity test and the serum albumin adsorption assay, the SOI surface displayed a significantly higher wetting velocity than the SA surface (P=0.000 and P=0.000, respectively). In the osteoblast adhesion, proliferation, differentiation, and mineralization tests, the mean values for SOI were all higher than those for SA and CA. On the osteoblast migration, platelet adhesion, and activation tests, SOI also showed significantly higher values than SA (P=0.040, P=0.000, and P=0.000, respectively). Conclusions: SOI exhibited higher hydrophilicity and affinity for proteins, cells, and platelets than SA. Within the limits of this study, it may be concluded that coating an implant with a pH buffering agent can induce the attachment of platelets, proteins, and cells to the implant surface. Further studies should be conducted to directly compare SOI with other conventional surfaces with regard to its safety and effectiveness in clinical settings.

• 한국환경생태학회지
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• 제25권4호
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• pp.540-561
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• 2011

지구온난화에 따른 한반도 식생구조의 변화에 맞추어 미황사 사찰림의 현 식생구조분석과 종다양성을 고려한 사찰림의 식생학적 구조분석과 적합한 식생구조 선정 및 관리를 위한 연구를 실시하였다. 본 연구는 식물사회학적 식생구조분석, 식생단위와 환경변수와의 상관관계, 생활형구조분석, 종다양성 분석 등을 실시하였다. 현 사찰림은 식물사회학적 식생구조 분석결과 졸참나무-굴참나무 군락, 붉가시나무군락, 팽나무군락의 식생단위로 구분되었으며, 환경변수 Ca, Mg, P 의 기준에 따라서 팽나무군락과 나머지 군락들(졸참나무-굴참나무군락과 붉가시나무군락)의 두 영역으로 분포하였다. 또한 환경변수 유기물(OM), Ni, Zn 에 따라서 졸참나무-굴참나무군락과 붉가시나무군락의 분포와 상관성이 있는 것으로 나타났다. 졸참나무-굴참나무 군락과는 양의 상관관계를 붉가시나무군락과는 음의 상관관계를 가지는 것으로 나타났다. 생활형 R1-2 식물인 조릿대가 관목층 이하에서 우점하고 있으며, 저층부의 종의 단순화를 이루고 있다. 종다양성은 졸참나무-굴참나무 군락이 붉가시나무 군락에 비하여 더 높게 나타났으며, 상록활엽수림으로 이루어진 남은사에 비해서 본 미황사 사찰림의 종다양성이 높게 나타났다. 식물사회학적 분석결과를 통한 식생군락, 조릿대에 의한 저층부 이하의 종의 단순화, 종다양성 및 종균재도를 고려한 미황사 사찰림의 장기적 식생관리가 필요하다 사료된다.