• Title/Summary/Keyword: S109

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Construction of Expression Vector of Bacillus sp. SSA3 Strain (Bacillus sp. SSA3 균주의 Expression Vector 개발)

  • 조윤래;김종규;권대준
    • Microbiology and Biotechnology Letters
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    • v.20 no.6
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    • pp.637-641
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    • 1992
  • The promoter regions from chromosomal DNA of Bacillus sp. SSA3 which is responsible for fermentation of Korean traditional soy sauce, were cloned for construction of expression vector of Bacills sp. SSA3. Recombinant plasmids were constructed by insertion of HindIIl-cleaved Bacillus sp. SSA3 chromosomal DNA fragments in front of the CAT gene of pGR71 plasmid and B-galactosidase gene of pUC18 plasmid. 6 recombinant plasmids were isolated from chloramphenicol resistant E. coli JM109 clones. All these plasmids were found to have promoter activity in Bacills sp. SSA3 and E. coli JM109. When these 6 clones of Bacills sp. SSA3 were cultivated in LB agar medium supplemented with 10% NaCI. fused CAT gene expression of 4 clones was significantly decreased in common. But the others were poorly inhibited.

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Luteolin Induced-growth Inhibition and Apoptosis of Human Esophageal Squamous Carcinoma Cell Line Eca109 Cells in vitro

  • Wang, Ting-Ting;Wang, Shao-Kang;Huang, Gui-Ling;Sun, Gui-Ju
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.11
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    • pp.5455-5461
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    • 2012
  • Luteolin is a plant flavonoid which exhibits anti-oxidative, anti-inflammatory and anti-tumor effects. However, the antiproliferative potential of luteolin is not fully understood. In this study, we investigated the effect of luteolin on cell cycling and apoptosis in human esophageal squamous carcinoma cell line Eca109 cells. MTT assays showed that luteolin had obvious cytotoxicity on Eca109 with an $IC_{50}$ of $70.7{\pm}1.72{\mu}M$ at 24h. Luteolin arrested cell cycle progression in the G0/G1 phase and prevented entry into S phase in a dose- and time-dependent manner. as assessed by FCM. Luteolin induced apoptosis of Eca109 cells was demonstrated by AO/EB staining assay and annexin V-FITC/PI staining. Moreover, luteolin downregulated the expression of cyclin D1, survivin and c-myc, and it also upregulated the expression of p53, in line with the fact that luteolin was able to inhibit Eca109 cell proliferation.

Must-have for Crowd-funding Projects: Credibility

  • Wang, Huaxin;Kim, Taekyung
    • Asia pacific journal of information systems
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    • v.26 no.1
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    • pp.109-123
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    • 2016
  • The success of a crowd-funding project can be attributed to various reasons, among which, backer's (also known as 'investor', or 'funder') perception of project credibility may be a salient one. The purpose of this study is to investigate the extent to which perceived project credibility can affect a backer's investment decision. We examine the factors that could influence the building of perceived project credibility by testing the proposed research model using survey data. Analysis results indicate that perceived project credibility has a significantly positive effect on backer's investment intention. Furthermore, information quality and source credibility are two key determinants of perceived project credibility. This study contributes to crowd-funding literature by enriching the list of successful factors for fund-raising with project credibility. The study also has practical implications because it explains why and how a backer's perception of project credibility can be improved.

Citrobacter amalonatics와 Citrobacter farmari에 의한 perchlorate 환원

  • Nirmala Bardiya
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2003.09a
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    • pp.438-441
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    • 2003
  • The present study reports the novel physiological function of dissimilatory perchlorate reduction by two strains JB101 and JB109 isolated from a sewage treatment facility in Incheon, South Korea. The physiological data of the isolates showed good correspondence with the members of the family Enterobacteriaceae. The partial 16S rRNA and 16S rDNA sequence of strains JB101 and JB109 showed similarity of 99.8% to Citrobacter amalonaticus and 98% to Citrobacter farmari, respectively. The study infers toward possibility of Citrobacter spp. to form an important group of dissimilatory perchlorate reducers within the (equation omitted) subclass of Proteobacteria because the majority of the known. members belong to two monophyletic groups namely Dechloromonas and Dechlorosoma in $\beta$ subclass of Proteobacteria.

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Serendipitous Functional Relations Deducible from Certain Generalized Triple Hypergeometric Functions

  • Choi, June-Sang;Hasanov, Anvar;Turaev, Mamasali
    • Kyungpook Mathematical Journal
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    • v.52 no.2
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    • pp.109-136
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    • 2012
  • We aim at presenting certain unexpected functional relations among various hypergeometric functions of one or several variables (for example, see the identities in Corollary 5) by making use of Carlson's method employed in his work (Some extensions of Lardner's relations between $_0F_3$ and Bessel functions, SIAM J. Math. Anal. 1(2)(1970), 232-242).

Sequence Analysis of the Internal Transcribed Spacer of Ribosomal DNA in the Genus Rhizopus

  • Park, You-Jung;Min, Byung-Re
    • Mycobiology
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    • v.33 no.2
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    • pp.109-112
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    • 2005
  • The internal transcribed spacer (ITS) regions including the 3'-end of 18S rRNA gene, 5.8S rRNA gene and the 5'-end of the 28S rRNA gene of Rhizopus spp. were amplified by PCR and analyzed by DNASIS program. Length polymorphism of these region ranged from 564 bp in R. oryzae to 789bp in R. stolonifer. The length and sequence of 5.8S was very conserved with $154{\sim}155\;bp$. The sequence of ITS2 was more variable than that of ITS1. The base substitution rates were ranged from 0 to 0.6069 per site, and higher rate was found in R. stolonifer. In general, transition was usually more frequent than transversion. On the basis of sequencing results, four groups were clustered with value of 61.9% similarity; R. oryzae, R. micros pores, R. homothallicus, and R. stolonifer groups.

The Elderly's Error Characteristics in Some Human Interactions (자극에 대한 상호작용 관점에서의 노인의 오류 특성)

  • Jung, Kwang-Tae
    • Journal of the Ergonomics Society of Korea
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    • v.30 no.1
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    • pp.109-115
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    • 2011
  • In this research, three topics were studied for the elderly. The first was the elderly's error characteristics for tracing task and the second was for depth perception. The characteristics were compared with the young's. Twenty two old people and twenty two young people participated in the tracing experiment, respectively. In the depth perception experiment, twenty three old people and twenty two young people participated. A depth perception apparatus and a tracing one were used in the experiment. In the depth perception error, a significant difference was not detected on gender. However significant differences were detected with age and viewing distance. In the tracing error, significant difference was detected with age, also. The third experiment was push-button operation and fifteen old people participated in the experiment. As the result, operation time error was increased with the button size and the moving distance. The result of this study will be able to be considered in its user interface design to reduce the elderly's error while using a product.

The Modern White Horse Temple and Online Reconfiguring of a Buddhist Heritage Space

  • Kai, SHMUSHKO
    • Journal of Daesoon Thought and the Religions of East Asia
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    • v.3 no.1
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    • pp.109-128
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    • 2023
  • Recent research shows that since the early 2000s, the Chinese Communist Party has increasingly used various cultural heritage sites, including Buddhist sites, as soft power agents. Furthermore, in the context of the Belt and Road Initiative, launched by the People's Republic of China, Buddhist temples, representatives, and practices have been harnessed to play a role in the state's agenda. In this context, White Horse Temple, as a feature of cultural tourism in Henan Province, is facing new opportunities and challenges. The article examines the material particularities of reconstructing the temple in light of this trajectory, building on materials retrieved at the site, and online representations of the temple. The author explores how the temple's unique spatiality and characteristics stress the use of soft power which harnesses online and offline cultural and popular trends for state agenda.