• 한국동물위생학회지
• /
• 제32권3호
• /
• pp.227-239
• /
• 2009

At the present study, it was aimed to detect virulence genes and antimicrobial resistance genes among 102 strains of 12 Salmonella serotypes isolated from pigs and cattle. In polymerase chain reaction (PCR), invA was detected from all strains of Salmonella spp., spvC was detected from Salmonella enterica serotype Enteritidis (S. Enteritidis) (100%), S. Bradenburg (75%), and S. Typhimurium (20.4%). Drug resistance related genes of 12 types were detected from all strains. TEM ($bla_{TEM}$) gene was detected from 51 (92.7%) of 55 $\beta$-lactams (54 ampicillin or 1 amoxicillin) resistance strains. 55 (100%) of 55 chloramphenicol resistance strains, 3 (100%) of 3 gentamicin resistance strains and 5 (100%) of 5 kanamycin resistance strains did contain cml, aadB, and aphA1-Iab, respectively. strB (89.9%), strA (88.4%), aadA2 (84.1%) and aadA1 (72.5%) were detected from 69 streptomycin resistance strains. sulII and dhfrXII were detected from 49 (100%) of 49 sulfamethoxazole/trimethoprim resistance strains, but sulI was not detected. tetA (97.9%) and tetB (21.6%) were detected from 97 tetracycline resistance strains. int gene was detected from 58 (56.9%) of 102 strains. 54 S. Typhimurium of 102 Salmonella spp. were attempted to detect drug resistance genes. TEM was detected from 44 (95.7%) of 46 $\beta$-lactams (45 ampicillin or 1 amoxicillin) resistance strains. cmlA was detected from 51 (100%) of 51 chloramphenicol resistance strains. aadA2 (100%), strA (100%), strB (100%), and aadA1 (79.6%) were detected from 54 streptomycin resistance strains. sulII (100%) and dhfrXII (100%) were detected from 49 sulfamethoxazole/trimethoprim resistance strains. tetA was detected from 54 (100%) of 54 tetracycline resistance strains. int gene was detected from 54 (100%) of 54 strains. The major drug resistance pattern and resistance gene profile were ampicillin, chloramphenicol, streptomycin, sulfamethoxazole/trimethoprim and tetracycline (ACSSuT) and TEM, cmlA, aadA1, aadA2, strA, strB, sulII, dhfrXII, tetA and int, respectively.

• Journal of the Optical Society of Korea
• /
• 제1권2호
• /
• pp.100-103
• /
• 1997

We perform degenerate and nondegenerate pump-probe experiments on bulk GaAs at 100 K below the band gap. We mostly observe a negative differential transmission signal both in the degenerate and nondegenerate experiments. We interpret our signal as due to two-photon absorption. This negative signal has a different origin from the normally considered band gap renormalization for resonant excitations.

• 한국통신학회논문지
• /
• 제33권11C호
• /
• pp.963-969
• /
• 2008

본 논문에서는 무선통신시스템의 수신단에 적용될 수 있는 6비트 100MHz 플래쉬 A/D 변환기를 설계하였다. 제안하는 플래쉬 A/D 변환기는 해상도가 1비트씩 증가함에 따라 2배수로 증가하는 S-R 래치 회로를 단순화하여 집적화 하였다. 기존 NAND 기반의 S-R 래치 회로에 사용되던 8개의 MOS 트랜지스터 숫자를 6개로 줄였으며, 비교단의 동적 소비전력을 최대 12.5%까지 감소되도록 설계하였다. 설계된 A/D 변환기는 $0.18{\mu}m$ CMOS n-well 1-poly 6-metal 공정을 사용하여 제작되었고, 전원 전압 1.8V, 샘플링 주파수 100MHz에서의 전력소모는 282mW이다. 입력 주파수 1.6MHz, 30MHz에서의 SFDR은 각각 35.027dBc, 31.253dBc이며, 4.8비트, 4.2비트의 ENOB를 나타내었다.

• 혜화의학회지
• /
• 제13권2호
• /
• pp.259-265
• /
• 2004

Objective; This study was designed to investigate the effect of Sa-am acupuncture and Arctii Fructus water extract on Post-herpetic Neuralgia. Method: We have treated them with acupuncture treatment of Sa-am acupuncture and Arctii Fructus water extract and evaluated the effect by Pain Rating Score(P.R.S.) and Visual Analogue Scale(V.A.S.) scale. Result: Before theraphy, the grades of Song's scale were P.R.S. 80 and V.A.S. 100, Kim's sclae's P.R.S. 77 and V.A.S. 100. After theraphy, the grades of song's scale were P.R.S. 5 and V.A.S. 10, Kim's scales's P.R.S. 10 and V.A.S. 10 Conclusion: The treatment of Sa-am-acupuncture and Arctii Fructus water extract on Post-herpetic Neuralgia was effective and will be attempted to the patients with it.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권2호
• /
• pp.917-923
• /
• 2014

Chondrosarcomas are malignant cartilage-forming tumors of bone which exhibit resistance to both chemotherapy and radiation treatment. miRNAs have been well demonstrated to regulate gene expression and play essential roles in a variety of biological processes, including proliferation, differentiation, migration, cell cycling and apoptosis. In this study, we obtained evidence that miR-100 acts as a tumor suppressor in human chondrosarcomas. Interestingly, cisplatin resistant chondrosarcoma cells exhibit decreased expression of miR-100 compared with parental cells. In addition, we identified mTOR as a direct target of miR-100. Overexpression of miR-100 complementary pairs to the 3' untranslated region (UTR) of mTOR, resulted in sensitization of cisplatin resistant cells to cisplatin. Moreover, recovery of the mTOR pathway by overexpression of S6K desensitized the chondrosarcoma cells to cisplatin, suggesting the miR-100-mediated sensitization to cisplatin dependent on inhibition of mTOR. In summary, the present studies highlight miR-100 as a tumor suppressor in chondrosarcoma contributing to anti-chemoresistance. Overexpression of miR-100 might be exploited as a therapeutic strategy along with cisplatin-based combined chemotherapy for the treatment of clinical chondrosarcoma patients.

• 한국토양동물학회지
• /
• 제2권2호
• /
• pp.76-82
• /
• 1997

Mortality of some agro-forest insect pests, Agrotis segetum, Blitopertha orientalis, Agerastica coerulea, Glyphodes perspectalis and Acantholyda parki caused by entomopathogenic nematodes was investigated in laboratory and in pot. Steinernema carpocapsae All and Pocheon strain were effective against 2nd of 3rd instar larvae of A. segetum showing 100% mortalities at the concentration of 10Ijs/larva but not effective against 4th inster larvae. Mortality of B. orientalis was 100% after 26 days in H. bacteriopora NC 1, H. bacteriophora Hamyang and S. glageri NC strain treatment. S. monticulum also showed 100% mortalities against A. coerulea andd G. perspectalis at the concentration of 80 and 40 Ijs/larva. However, H. bacteriophora Cheju and S. glaseri Cheju strain were not effective against A. parki, i.e., showing 23.3 and 20.0% mortalities, respectively at the concentration of 160Ijs/larva. S. glaseri Hanrim strain was more effective than H. bacteriophora Cheju strain against Pryeria sinica.

• Bulletin of the Korean Chemical Society
• /
• 제31권8호
• /
• pp.2247-2254
• /
• 2010

The surface structures of copper phthalocyanine (CuPc) thin films deposited on sulphur-passivated and plane perylene-3,4,9,10-tetracarboxylic dianhydride (PTCDA)-covered InAs(100) surfaces have been studied by low energy electron diffraction (LEED) and van der Waals (vdW) intermolecular interaction energy calculations. The annealing to $300^{\circ}C$ and $450^{\circ}C$ of $(NH_4)_2S_x$-treated InAs(100) substrates produces a ($1{\times}1$) and ($2{\times}1$) S-passivated surface respectively. The CuPc deposition onto the PTCDA-covered InAs(100) surface leads to a ring-like diffraction pattern, indicating that the 2D ordered overlayer exists and the structure is dominantly determined by the intermolecular interactions rather than substrate-molecule interactions. However, no ordered LEED patterns were observed for the CuPc on S-passivated InAs(100) surface. The intermolecular interaction energy calculations have been carried out to rationalise this structural difference. In the case of CuPc unit cells on PTCDA layer, the planar layered CuPc structure is more stable than the $\alpha$-herringbone structure, consistent with the experimental LEED results. For CuPc unit cells on a S-($1{\times}1$) layer, however, the $\alpha$-herringbone structure is more stable than the planar layered structure, consistent with the absence of diffraction pattern. The results show that the lattice structure during the initial stages of thin film growth is influenced strongly by the intermolecular interactions at the interface.

• Asian-Australasian Journal of Animal Sciences
• /
• 제25권1호
• /
• pp.44-51
• /
• 2012

Calcium ions play an important role in the establishment and maintenance of pregnancy, but molecular and cellular regulatory mechanisms of calcium ion action in the uterine endometrium are not fully understood in pigs. Previously, we have shown that calcium regulatory molecules, transient receptor potential vanilloid type 5 (TRPV6) and calbindin-D9k (S100G), are expressed in the uterine endometrium during the estrous cycle and pregnancy in a pregnancy status- and stage-specific manner, and that estrogen of conceptus origin increases endometrial TRPV6 expression. However, regulation of S100G expression in the uterine endometrium and conceptus expression of S100G has been not determined during early pregnancy. Thus, we investigated regulation of S100G expression by estrogen and interleukin-$1{\beta}$ (IL1B) in the uterine endometrium and conceptus expression of S100G during early pregnancy in pigs. We obtained uterine endometrial tissues from day (D) 12 of the estrous cycle and treated with combinations of steroid hormones, estradiol-$17{\beta}$ ($E_2$) and progesterone ($P_4$), and increasing doses of IL1B. Real-time RT-PCR analysis showed that $E_2$ and IL1B increased S100G mRNA levels in the uterine endometrium, and conceptuses expressed S100G mRNA during early pregnancy, as determined by RT-PCR analysis. To determine if endometrial expression of S100G mRNA during the implantation period was affected by the somatic cell nuclear transfer (SCNT) procedure, we compared S100G mRNA levels in the uterine endometrium from gilts with SCNT-derived conceptuses with those from gilts with conceptuses derived from natural mating on D12 of pregnancy. Real-time RT-PCR analysis showed that levels of S100G mRNA in the uterine endometrium from gilts carrying SCNT-derived conceptuses was significantly lower than those from gilts carrying conceptuses derived from natural mating. These results showed that S100G expression in the uterine endometrium was regulated by estrogen and IL1B of conceptus origin, and affected by the SCNT procedure during early pregnancy. These suggest that conceptus signals regulate S100G, an intracellular calcium transport protein, for the establishment of pregnancy in pigs.

• 한국수정란이식학회지
• /
• 제21권3호
• /
• pp.233-239
• /
• 2006

본 연구는 후산정체 젖소에 있어서 Lipopolysaccharide의 처리가 자궁 회복에 미치는 영향을 조사하기 위하여 2004년부터 2005년까지 2년간에 걸쳐 축산연구소와 전문 경영체 농장에서 사육중인 홀스타인 착유우 중 분만 후 12시간이 경과하여도 태반이 배출되지 않았던 후산정체우 33두를 대상으로 LPS $100{\mu}g$구, LPS $200{\mu}g$구 및 대조구 각각 11두씩을 공시하였으며 분만 후 20일째에 대조구는 PBS를, 처치구는 LPS를 자궁내에 주입하여 분만 후 40일째에 초음파 측정을 통하여 자궁의 크기 및 자궁내 삼출물의 저류 정도를 조사하여 다음과 같은 결과를 얻었다. 1.자궁각의 크기에 있어서 30 mm이하인 경우는 대조구 45.5%, LPS $100{\mu}g$구 100%, LPS $200{\mu}g$구 72.7%였고 $31{\sim}50mm$인 경우는 대조구 27.3%, LPS $100{\mu}g$구 0%, LPS $100{\mu}g$구 18.2%였으며 51 mm이상인 경우는 대조구 27.3%, LPS $100{\mu}g$구 0%, LPS $200{\mu}g$구 9.1%였다. 2. 삼출액의 저류 상태에 있어서 삼출액이 거의 없는 경우는 대조구 18.2%, LPS $100{\mu}g$구63.6%, LPS $200{\mu}g$구 54.6%였고 자궁강내에 삼출액이 소량 있는 경우는 대조구 36.4%, LPS $100{\mu}g$구 36.4%, LPS $200{\mu}g$구 36.4%였으며 자궁강내에 삼출액이 고여 있는 경우는 대조구 45.5%, LPS $100{\mu}g$구 0%, LPS $200{\mu}g$구 9.1%였다.

• Bulletin of the Korean Chemical Society
• /
• 제35권2호
• /
• pp.581-586
• /
• 2014

The most stable adsorption structures and their corresponding energies of 4-pyridone, 4-hydroxypyridine, 2-pyridone and 2-hydroxypyridine have been investigated by Density Functional Theory (DFT) calculation and high-resolution photoemission spectroscopy (HRPES). We confirmed that between the two reaction centers of 4- and 2-pyridone, only O atom of carbonyl functional group can act as a Lewis base while both the two reaction centers of 4- and 2-hydroxypyridine (tautomers of 4- and 2-pyridone) can successfully function as a Lewis base. On the other hand, owing to their molecular structures, there is a remarkable difference between the adsorption structures of 4- and 2-hydroxypyridine. Through the analysis of the N 1s and O 1s core level spectra obtained using HRPES, we also could corroborate that two different adducts coexist on the surface at room temperature due to their activation energy investigating the coverage dependent variation of bonding configurations when these molecules are adsorbed on the Ge(100) surface.