• Journal of Multimedia Information System
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• 제8권3호
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• pp.159-166
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• 2021

COVID-19 has dramatically changed people's daily life. Wearing masks is considered as a simple but effective way to defend the spread of the epidemic. Hence, a real-time and accurate mask wearing detection system is important. In this paper, a deep learning-based mask wearing detection system is developed to help people defend against the terrible epidemic. The system consists of three important functions, which are image detection, video detection and real-time detection. To keep a high detection rate, a deep learning-based method is adopted to detect masks. Unfortunately, according to the suddenness of the epidemic, the mask wearing dataset is scarce, so a mask wearing dataset is collected in this paper. Besides, to reduce the computational cost and runtime, a simple online and real-time tracking method is adopted to achieve video detection and monitoring. Furthermore, a function is implemented to call the camera to real-time achieve mask wearing detection. The sufficient results have shown that the developed system can perform well in the mask wearing detection task. The precision, recall, mAP and F1 can achieve 86.6%, 96.7%, 96.2% and 91.4%, respectively.

• 한국수산과학회지
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• 제28권1호
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• pp.15-22
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• 1995

새로운 식중독 원인균으로 알려지고 있는 V cholerae non-O1과 V mimicus의 분포와 서식 조건을 밝히고자 금강 하구에서 1993년 3월 26일부터 1994년 2월 22일까지 수질 환경과 V cholerae non-O1 및 V mimicus의 분포를 조사한 결과는 다음과 같다. 1. V cholerae non-O1은 해수 54개 시료에서 $16.7\%$, 뻘 49개 시료에서 $6.1\%$, 전체 $11.7\%$가 검출되었으며 V mimicus는 해수 54개 시료에서 $11.1\%$, 뻘 49개 시료에서 $10.2\%$, 전체 $10.7\%$가 검출되었다. 2. V cholerae non-O1과 V. mimicus는 수온 $24^{\circ}C$이상, 염도 $10\%o$ 이하의 기수에서 주로 검출되었으나, 수온이 $3.5^{\circ}C$인 1월에 뻘에서 검출되었다. 3. V cholerae non-O1과 V. mimicus의 서식에 영향을 주는 주요 인자는 온도, 염도 및 유기물질의 오염이며, V. cholerae non-O1은 V. mimicus보다 염도 변화에 대한 적응력이 더 높았다.

• Journal of Microbiology and Biotechnology
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• 제9권2호
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• pp.184-190
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• 1999

The use of the polymerase chain reaction (PCR) method was described using two sets of primers based on the ceuN gene (JEJ 1 and JEJ 2) which encodes a protein involved in siderophore transport and 16S rRNA gene (pA and pB) for the sensitive and specific detection of enteropathogen Campylobacter jejuni. Six oligonucleotides were utilized in an amplification experiment and PCR products of predicted sizes were generated from whole cells and boiled cell lysates at the same intensity. Two sets of the primer pairs, JEJ and pAB, were specific enough for all C. jejuni strains tested for the direct use of whole cells without DNA extraction or lysis steps. In the PCR using the pAB primer pair, the detection limit, as determined by the ethidium bromide staining of the amplification products on agarose gels, was at the level of $10^1$ bacteria cells or less in both the pure culture and artificially inoculated milk and chicken enrichment samples, whereas the detection limit with the JEJ primer pair was relatively low, i.e. $10^3$ cells or more in the same PCR samples. The PCR method using either a primer JEJ or pAB was both repeatable and specific for the detection of C. jejuni in food. This method is simply completed within 4 h.

• 대한수의학회지
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• 제13권1호
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• pp.39-46
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• 1973

Cultural method, dark field microscopy & fluorescent antibody technique were compared for their sensitivity of the detection of leptospires from experimentally infected mice. Two groups of mice were infected with L. icterohemorrhagiae (M20) and L. australis (Ballico), and the infected blood, urine and a number of organs were subjected to the bacterial isolation. The results obtained were summarized as follows: 1. L. icterohemorrhagiae (M20) and L. australis (Ballico) in blood, urine and various tissues of experimentally infected mice were detected with a negrigible non specificity, by the fluorescent antibody technique. 2. The fluorescent antibody technique, as applied to detection of leptospires in blood, urine and various infected tissue, proved to be better than cultural method and dark-field microscopy. 3. Early detection of leptospires by fluorescent antibody technique were possible in blood at 2 days after inoculation, whereas detection of organisms in liver, spleen, lung and kidney were observed later. By means of fluorescent antibody technique, the detection of leptospires in kidney and urine was possible up to 34 days postinoculation, whereas those in other parts were impossible. 4. Fluorescent antibody reaction of leptospires were highly specific to homologous antigen rather than to heterologous one. 5. Fluorescent antibody technique may be of value in the application for the demonstration of leptospira from clinical specimens.

• 중소기업융합학회논문지
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• 제3권1호
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• pp.31-41
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• 2013

치명적인 사고를 막기 위해 드라이버 졸음 (DD)를 검출하는 다양한 최근 방법이 제안되고있다. 본 논문은 운전자의 눈에 폐쇄 속도를 모니터링 할 수 있는 기능을 AdaBoost 기반 물체 검출 알고리즘에 적용한 DD 탐지 시스템 구현에서 하드웨어/소프트웨어 공동 설계 방법을 제안한다. 소프트웨어 구성 요소는 DD 검출 알고리즘 중에서 필요한 기능성을 완전하게 달성하기 위해 전체적인 제어 및 논리 연산을 구현한다. 반면, 본 연구에서는 DD 검출 알고리즘의 중요한 기능은 처리를 가속화하기 위해 맞춤형 하드웨어 구성 요소를 통해 가속된다. 하드웨어/소프트웨어 아키텍처는 비디오 도터 보드와 알테라 DE2 보드에 구현되었습니다. 제안 된 구현의 성능을 평가하고 몇 가지 최근의 작품을 벤치마킹했다.

• 대한수의학회지
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• 제43권2호
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• pp.239-246
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• 2003

Polymerase chain reaction (PCR) was evaluated for the early detection of Aujeszky's disease virus (ADV) DNA from virus-infected cell cultures. For the purposes, the Korean ADV NYJ1-87 was propagated in swine kidney (SK) cells and subjected to the amplification of DNA (217 bp) by PCR using sense and antisense primers specific to gp50 gene of the ADV. In detection of cell-associated viral DNA, reliable PCR conditions were determined as 30 cycles of reaction consisting 1 minute each of denaturation at $94^{\circ}C$, annealing at $55^{\circ}C$ and polymerization at $72^{\circ}C$. The PCR encountered best results with reagent mixtures of $50{\mu}l$ containing $200{\mu}M$ dNTPs, $0.2{\mu}M$ each sense and antisense primers, 1 mM $MgCl_2$ and 10% (v/v) template DNA in the final concentrations. ADV-specific DNAs were detected as early as 6, 6, and 9 hours post-infection, respectively, from lysates of the SK cells infected with ADV of $10^3$, $10^2$ and $10^1\;TCID_{50}/ml$ by this condition. In culture supernatant, the DNAs were detected from ADV of as low infectivity as $10^ {-3}\;TCID_{50}/ml$ by the reduced reagent concentrations and 30 cycles of 1 minute each of denaturation at $94^{\circ}C$ and annealing at $55^{\circ}C$, and 2 minutes of polymerization at $72^{\circ}C$. The lowest amount of detectable ADV DNA was 1 fg. In conclusion, the PCR condition established in the present study was recognized as a feasible alternative to time-consuming procedures in isolation and characterization of the virus.

• Bulletin of the Korean Chemical Society
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• 제27권2호
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• pp.231-236
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• 2006

A novel sample pretreatment technique, headspace hanging drop liquid phase microextraction (HS-LPME) was studied and applied to the determination of flavors from solid clove buds by gas chromatography-mass spectrometry (GC-MS). Several parameters affecting on HS-LPME such as organic solvent drop volume, extraction time, extraction temperature and phase ratio were investigated. 1-Octanol was selected as the extracting solvent, drop size was fixed to 0.6 $\mu$L. 60 min extraction time at 25 ${^{\circ}C}$ was chosen. HS-LPME has the good efficiency demonstrated by the higher partition equilibrium constant ($K_{lh}$) values and concentration factor (CF) values. The limits of detection (LOD) were 1.5-3.2 ng. The amounts of eugenol, $\beta$-caryophyllene and eugenol acetate from the clove bud sample were 1.90 mg/g, 1.47 mg/g and 7.0 mg/g, respectively. This hanging drop based method is a simple, fast and easy sample enrichment technique using minimal solvent. HSLPME is an alternative sample preparation method for the analysis of volatile aroma compounds by GC-MS.

• 한국방재안전학회논문집
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• 제10권2호
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• pp.1-6
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• 2017

사물 인터넷(IOT)을 기반으로 한 지하철 재난 조기경보 및 승객 대피를 위한 재난전조 시스템 개발 연구가 이미 선행연구를 통해 수행하였다. 본 논문에서는 역에 설치된 센서 데이터를 분석하여 재난을 신속 하게 감지하기 위한 후속 연구이다. 특히, 본 연구는 초기 시스템 구축 시 센서의 설치 위치에 따라 다를 수 있는 환경변화를 고려한 마할라노비스 거리를 기반으로 통계적 방법론을 개발하였다.

• 한국통신학회논문지
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• 제28권5B호
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• pp.489-499
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• 2003

본 논문은 CPN(Colored Petri Nets)을 이용한 Honypot 모델의 설계 및 구현에 관한 것이다. 제안된 Honeypot 모델은 해커의 침입을 능동적으로 유도하고 침입을 탐지 및 행동패턴의 파악을 위해 보안커널 모듈과 유도된 해커의 활동을 위한 가상 모듈로 구성되어 있으며, CPN을 이용한 모델과 기존의 Denning 모델 및 Shieh 모델과 비교 분석하였다. 본 논문에서 제안된 CPN을 이용한 Honeypot 모델은 침입패턴의 특성에 대한 분류가 가능하고, 침입패턴의 모델링과 패턴매칭 과정의 모델링이 가능하며. 다중 호스트를 통한 DDoS 공격의 탐지가 가능하고, 마지막으로 침입패턴의 분석을 위한 학습모델의 기반 제공이 가능하다.

• Bulletin of the Korean Chemical Society
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• 제31권11호
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• pp.3077-3083
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• 2010

The electrochemical detection of As(III) was investigated on a platinum-iron(III) nanoparticles modified multiwalled carbon nanotube on glassy carbon electrode(nanoPt-Fe(III)/MWCNT/GCE) in 0.1 M $H_2SO_4$. The nanoPt-Fe(III)/MWCNT/GCE was prepared via continuous potential cycling in the range from -0.8 to 0.7 V (vs. Ag/AgCl), in 0.1 M KCl solution containing 0.9 mM $K_2PtCl_6$ and 0.6 mM $FeCl_3$. The Pt nanoparticles and iron oxide were co-electrodeposited into the MWCNT-Nafion composite film on GCE. The resulting electrode was examined by cyclic voltammetry (CV), scanning electron microscopy (SEM), and anodic stripping voltammetry (ASV). For the detection of As(III), the nanoPt-Fe(III)/MWCNT/GCE showed low detection limit of 10 nM (0.75 ppb) and high sensitivity of $4.76\;{\mu}A{\mu}M^{-1}$, while the World Health Organization's guideline value of arsenic for drinking water is 10 ppb. It is worth to note that the electrode presents no interference from copper ion, which is the most serious interfering species in arsenic detection.