• Title/Summary/Keyword: S-transformation

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Transformation of Bacillus Subtilis by Streptomyces bobili R-Plasmid DNA (Streptomyces bobili의 R-Plasmid. DNA에 의한 Bacillus subtilis의 Transformation)

  • 김상달;도재호
    • Microbiology and Biotechnology Letters
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    • v.11 no.3
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    • pp.163-168
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    • 1983
  • The penicillin resistant plasmid DNA was prepared from Streptomyces bobili YS-40, producing penicillinase, by the phenol extraction method and introduced into Bocillus subtilis IAM 12118 by the transformation procedure of Mahler method. The optimal pH and temperature on the transformation was 7.0, 3$0^{\circ}C$ respectively. Above 20 minutes contact of plasmid DNA and recipient cell was shown the high transformation frequency. The transformant of penicillin resistance was proportionally increased as increase of the DNA concentration. The addition of lysine in transformation system increased the transformation frequency about 6-fold and the addition of the chloramphenicol did not affect the transformation frequency.

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Impact of Digital Transformation on Business Performance: Moderating Role of Innovation Resistance and Organizational Characteristics

  • Jin-Kwon KIM;Min-Chul KIM;Tony-DongHui AHN
    • The Journal of Economics, Marketing and Management
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    • v.12 no.4
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    • pp.65-76
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    • 2024
  • Purpose: This study aims to identify the relationship between SMEs' digital transformation capabilities, smart factory utilization, and management performance. It also aims to suggest how companies strategically utilize smart factories to achieve a competitive advantage and sustainable growth through empirical analysis of differences in innovation resistance and organizational characteristics. Research design, data, and methodology: This study Implement for SME's building smart factories did. The survey was conducted for 90days from October 1st, 2023 to December 31th, 2023. Total of 210 surveys were collected, and 186 surveys, excluding ones with missing value and outliers (64 surveys), were used. Results: The results of the empirical analysis based on previous research are as follows. First, digital transformation capabilities such as digital technology, digital leadership, and digital strategy affect smart factory utilization. Second, smart factory use affects operational performance. Third, innovation resistance has a moderating effect in the relationship with digital transformation capabilities, smart factory utilization, and management performance. Fourth, organizational characteristics have a moderating effect in the relationship with digital transformation capabilities, smart factory utilization, and management performance. Conclusions: Explore strategic ways to improve your organization's digital transformation capabilities. It is necessary to establish a strategy to make organizational members aware of the necessity and importance of introducing a new system through centralization of the organization.

Image registration using outlier removal and triangulation-based local transformation (이상치 제거와 삼각망 기반의 지역 변환을 이용한 영상 등록)

  • Ye, Chul-Soo
    • Korean Journal of Remote Sensing
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    • v.30 no.6
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    • pp.787-795
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    • 2014
  • This paper presents an image registration using Triangulation-based Local Transformation (TLT) applied to the remaining matched points after elimination of the matched points with gross error. The corners extracted using geometric mean-based corner detector are matched using Pearson's correlation coefficient and then accepted as initial matched points only when they satisfy the Left-Right Consistency (LRC) check. We finally accept the remaining matched points whose RANdom SAmple Consensus (RANSAC)-based global transformation (RGT) errors are smaller than a predefined outlier threshold. After Delaunay triangulated irregular networks (TINs) are created using the final matched points on reference and sensed images, respectively, affine transformation is applied to every corresponding triangle and then all the inner pixels of the triangles on the sensed image are transformed to the reference image coordinate. The proposed algorithm was tested using KOMPSAT-2 images and the results showed higher image registration accuracy than the RANSAC-based global transformation.

TRANSFORMATION OF DIMENSIONLESS HEAT DIFFUSION EQUATION FOR THE SOLUTION OF DYNAMIC DOMAIN IN PHASE CHANGE PROBLEMS

  • Ashraf, Muhammad;Avila, R.;Raza, S. S.
    • Journal of the Korean Society for Industrial and Applied Mathematics
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    • v.13 no.1
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    • pp.31-40
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    • 2009
  • In the present work transformation of dimensionless heat diffusion equation for the solution of moving boundary problems have been formulated. The formulation is based on 1-D, 2-D and 3-D, unsteady heat diffusion equations. These equations are rst turned int dimensionless form by using dimensionless quantities and their transformation was formulated in liquid and solid phases. The salient feature of this work is that during the transformation of dimensionless heat diffusion equation there arises a convective term $\tilde{v}$ which is responsible for the motion of interface in liquid as well as solid phase. In the transformed heat equation, a correction factor $\beta$ also arises naturally which gives the correct transformed flux at interface.

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Dilatometry Analysis of Low Carbon Steel considering Transformation Mismatch Plasticity (변태소성을 고려한 저탄소강 상변태의 Dilatometry 해석)

  • Suh D. W.;Oh C. S.;Kim S. J.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2005.10a
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    • pp.332-335
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    • 2005
  • A model is developed to quantitatively analyze the dilatometry curve of carbon steel for the evaluation of phase fraction during transformation. The effect of anisotropic volume change due to transformation mismatch plasticity as well as carbon enrichment in austenite during the transformation is considered in the developed model. The developed model is applied for the analysis of dilatometry curves of carbon steels. The result shows that considering the anisotropic dilatation is very essential to quantitatively evaluate the phase fraction from the dilatation curve.

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Transformation expressed in Dress (Part I) (복식에 표현된 트랜스포메이션에 관한 연구 (제1보))

  • Na, Young-Won;Park, Myung-Hee
    • Journal of the Korean Society of Clothing and Textiles
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    • v.30 no.1 s.149
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    • pp.167-175
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    • 2006
  • The purpose of this study is to clarify the expansion of functions of clothes by analyzing the characteristics of transformation, and to forecast future trends in fashion through systematization of the aforementioned analyses. Analysis of 20th century Modernism and Post-Modernism in a sociocultural sense confirms that transformation in clothes was formed by environmental, functional, deconstructive, and expressive factors. In this sense, the formative factors mentioned above conceptually include nomadic characteristics, usefulness, irregularity, and expressiveness. The nomadic characteristics found in clothes transformation signify the change of clothes into environmental nomadic everyday implements, used as tools for the body. Usefulness of clothes means that it is worn for variability, multipurpose multi-functionality, and combined multiple use. Irregularity means the clothes can change indefinitely, according to random manipulation on the wearer's part. Last of all, expressiveness conveys the designer's internal sensitivity and imagination onto an external object through the induction of various expressive factors.

Calculation of Jominy Hardenability Curve of Low Alloy Steels from TTT/CCT data (TTT/CCT 데이터를 이용한 저합금강의 죠미니 경화능 곡선 계산)

  • Jung, Minsu;Son, YoonHo
    • Journal of the Korean Society for Heat Treatment
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    • v.32 no.1
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    • pp.17-28
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    • 2019
  • Jominy hardenability curves of low alloy steel containing less than 5 wt.% of alloying elements in total were calculated by applying Scheil's rule of additivity to pre-calculated isothermal transformation curve. Isothermal transformation curve for each phase in steel was approximated as a simple mathematical equation by using Kirkaldy's approach and all coefficients in the equation were estimated from experimental temperature-time-transformation (TTT) and/or continuous cooling transformation (CCT) data in the literature. Then jominy test with simple boundary conditions was performed in computer by applying the finite difference scheme. The resultant cooling curves at each location along a longitudinal direction of Jominy bar were applied to calculate phase fractions as well as mechanical properties such as micro Vickers hardness. The simulated results were compared with experimental CCT data and Jominy curves in the literature.

Preemptive or Catch Up? Performance Differences under Enterprise Digital Transformation

  • Peinan Ji;Guang Yu
    • Asia pacific journal of information systems
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    • v.32 no.3
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    • pp.564-579
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    • 2022
  • The use of on-premises technology in the business environment to create a competitive advantage is ushering in a new era known as digital transformation. As the foundation of digital transformation of enterprises, information technology still has a paradoxical effect on enterprises. This paper documents the effect of investments in IT on a firm's long-term profitability performance measures as return on assets (ROA), as well as tests whether the earlier entrant and the later entrant are different in IT investment performance. Using a sample of China's public firms IT investment data between 2016 and 2019, the result indicates that IT investment in firms have a positive effect on firm performance in full sample, but not in the financial industry firms. When it comes to the different investment time, the result shows no significant difference between the earlier entrant firm and the later entrant firm in the full sample, but not in the case of software industry sample. This should help alleviate the concerns that some have expressed about the viability of digital transformation given the highly publicized IT investment and implementation problems at some firms.

Color Transformation of Images based on Emotion Using Interactive Genetic Algorithm (대화형 유전자 알고리즘을 이용한 감정 기반 영상의 색변환)

  • Woo, Hye-Yoon;Kang, Hang-Bong
    • The KIPS Transactions:PartB
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    • v.17B no.2
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    • pp.169-176
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    • 2010
  • This paper proposes color transformation of images based on user's preference. Traditional color transformation method transforms only hue based on existing templates that define range of harmonious hue. It does not change saturation and intensity. Users would appreciate the resulting images that adjusted unnatural hue of images. Since color is closely related to peoples' emotion, we can enhance interaction of emotion-based contents and technologies. Therefore, in this paper, we define the range of color of each emotion for the transformation of color and perform the transformation of hue, saturation and intensity. However, the relationship of color and emotion depends on the culture and environment. To reflect these characteristics in color transformation, we propose the transformation of color that is based on user's preference and as a result, people would be more satisfied. We adopt interactive genetic algorithm to learn about user's preference. We surveyed the subject to analyze user's satisfaction about transformed images that are based on preference, and we found that people prefer transformed images to original images. Therefore, we conclude that people are more satisfied with the transformation of the templates which reflected user's preference than the one that did not.

Heterologous Transformation of Saccharomyces cerevisiae by Glucoamylase Gene of Saccharomyces diastaticus (Saccharomyces diastaticus Glucoamylase Gene에 의한 Saccharomyces cerevisiae의 Transformation)

  • Kim, Young-Ho;Jun, Do-Youn;Seu, Jung-Hwn
    • Microbiology and Biotechnology Letters
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    • v.16 no.6
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    • pp.489-493
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    • 1988
  • To obtain a new yeast strain that is able to efficiently produce ethanol from starch, the glucoamylase gene of Saccharomyces diastaticus was transformed into S. cerevisiae without a cloning vector. The competent cells of S. cerevisiae, induced by the treatment of Li$_2$SO$_4$, were transformed with the partial BamHI-digests of chromosomal DNA of S. diastaticus, and the transformants were selected by their abilities to utilize and ferment starch. The transformants, which appeared at a frequency of 8.5$\times$10$^{-7}$, were able to withstand up to 800 ppm of copper sulfate like the recipient and retained the phenotypic expression of the recipient with the exception of the acquisition of STA gene and MAL gene, as regards fermentation of carbohydrates. The enzymatic properties of glucoamylases produced by transformants were very similar to those produced by S. diastaticus as based on optimium pH and temperature.

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