• 제목/요약/키워드: S-oligo

검색결과 83건 처리시간 0.026초

Polycystic Ovary Syndrome and Risk of Endometrial Cancer: a Mini-Review

  • Tokmak, Aytekin;Kokanali, Mahmut Kuntay;Guzel, Ali Irfan;Kara, Aydan;Topcu, Hasan Onur;Cavkaytar, Sabri
    • Asian Pacific Journal of Cancer Prevention
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    • 제15권17호
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    • pp.7011-7014
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    • 2014
  • The polycystic ovary syndrome is the most common endocrinological disorder of reproductive age women with a prevalence of 5 to 8 %. The most common diagnostic criteria used for polycystic ovary syndrome are oligo- or an-ovulation, clinical and/ or biochemical signs of hyperandrogenism and polycystic ovaries. Hyperandrogenism results in increased estrogen levels and lack of cyclic progesterone due to anovulation and persistent stimulation of the endometrium may lead to endometrial hyperplasia or adenocarcinoma development. In this mini review, we aimed to evaluate the possible relationship between polycystic ovary syndrome and endometrial cancer.

Gene Targeting in Mouse Embryos Mediated by recA and Modified Single-Stranded Oligonucleotides

  • Kang, Jee-Hyun;Won, Ji-Young;Heo, Soon-Young;Hosup Shim
    • 한국동물번식학회:학술대회논문집
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    • 한국동물번식학회 2004년도 춘계학술발표대회
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    • pp.193-193
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    • 2004
  • Gene targeting is an in situ manipulation of endogenous gene with precise manner by the introduction of exogenous DNA. The process of gene targeting involves a homologous recombination reaction between the targeted genomic sequence and an exogenous targeting vector. In elucidating the function of many genes, gene targeting has become the most important method of choice. (omitted)

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Nucleotide Sequence of Leghemoglobin cDNA from Canavalia lineata

  • An, Chung-Sun
    • Journal of Plant Biology
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    • 제37권2호
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    • pp.167-173
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    • 1994
  • Poly(A)+ RNA was selected from Canavalia lineata root nodule RNA through oligo(dT) cellulose column and used for construction of a cDNA library using λgt10-EcoRI arms. The size of the library was 7.2$\times$105 pfu/mL. A full length leghemoglobin (Lb) cDNA clone, pCILb1(687 bp) isolated with soybean Lb probe, contained one open reading frame (ORF) of 447 bp with 54 bp plus 186 bp at 5' and 3' untranslated region, respectively. A consensus sequence of plant translation start region (AAAATGGG) was found at 5' untranslated region, and two polyadenylation-related sequence (AATAAA, AATAAG) and a conserved motif between them (gACTTGTT) were found upstream of poly(A)+ tail consisted of 13 (A)s at 3' untranslated region. The ORF encoded a polypeptide consisted of 149 amino acids with a molecular weight of 16.2 kD. Deduced amino acid sequences showed high degree of homology values with those of other Lbs ranging from 66% (Casuarina glauca) to 85% (Glycine max).

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배내천 中流의 底棲性 大形無脊椎動物에 대한 硏究 1. 群集分析 및 生物學的 水質 判定 (Study on the Benthic Macroinvertebrates in the Middle Reaches of the Paenae Stream , a Tributary of the Naktong River , Korea)

  • Oh, Yong-Nam;Tae-Soo Dhon
    • The Korean Journal of Ecology
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    • 제14권3호
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    • pp.345-360
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    • 1991
  • Benthic macroinvertebrates were collecte in the middle reaches of the paenae stream, a tributary of th naktong river in korea, in four seasons from August 1989. To may 1990, during the study period, 125 species, 81 genera and 41 families were collected. Overall, the most dominant species was lepidostoma sp. 1 followed by heptagenia kihada, neoperla quadrata, diamesa sp. 1, ect.. the community was most diverse in autumn, However the number of collectd individuals was highest in winter. Species of chironomids including diamesa sp. 1 and mayflies including paraleptophlebia chocorata were collected exceptionally in a large number in winter. Seasonal occurrence of funtional groups such as collectors, shredders and scrapers was related with the availadlity of food sourses in the stream. The abundance of predators was relatively stable in four seasons with 17% on average. Diversity, tbi and chandler's biotic score(bs) in the paenae strem were 3.75, 14.5, and 100 on average, respectively. Based on biological and chemical indices the paenae stream was clean in the range of xeno- and oligo- saprobity.

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Pseudomonas sp. $\beta$-1,4-Glucosidase 유전자의 Esherichia coli와 Bacillus subtilis에의 Cloning 및 발현 (Cloning and Expression of $\beta$-l,4-Glucosidase Gene from Pseudomonas sp. in Escherichia coli and Bacillus subtilis)

  • 김양우;전성식;김석재;정영철;성낙계
    • 한국미생물·생명공학회지
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    • 제21권2호
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    • pp.113-118
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    • 1993
  • Fro the purpose of producing glouse from cellobiose or oligo saccharide and obtaining genetic information of beta-1,4-glucosidase gene, alpha beta-1,4-glucosidase gene of Pseudomonas sp. LBC505, potent cellulase complex and xylanase producing strain, was cloned in Esherichia coli and Bacillus subtilis into pUC19 and pBD64, respectively. Recombinant plasmid pGL1 contained 1.2kb EcoRI fragment was isolated from transformants forming blue color around colony on LB agar plate containing 20 ng/ml of 5-bromo-4-chloro-3-indolyl-beta-D-glucopyranoside(X-glu) and ampicillin.

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Preparation of Copolymers by Controlled Radical Polymerization and their Applications

  • Choi, Kil-Yeong;Kim, Yong-Seok;Han, Seung-San;Lee, Jae-Heung
    • 한국고분자학회:학술대회논문집
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    • 한국고분자학회 2006년도 IUPAC International Symposium on Advanced Polymers for Emerging Technologies
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    • pp.95-96
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    • 2006
  • Surface modification of clay minerals has become increasingly important for improving the practical applications of clays such as polymeric nanocomposites. We used the copolymer as modifiers having phenyl components, and successfully developed a route for the preparation of amine functionalized polymer based on oligostyrene and its block copolymers. The oligo(St-co-VBC)s with controlled molecular weight were synthesized via nitroxide mediated polymerization method. We also successfully prepared organophilic layered silicates whose surface is covered with styrenic copolymers. Through the analysis of chemical structure and morphology, we concluded that copolymers were very effective organic modifiers to change the surface characteristics of layered silicates.

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Synthesis and Properties of Triblock and Multiblock Copolymers Consisting of Poly(L-lactide) and Poly(oxyethylene-co-oxypropylene)

  • Lee, Chan-Woo;Kang, Young-Goo;Kun Jun
    • Macromolecular Research
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    • 제9권2호
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    • pp.84-91
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    • 2001
  • Both A-B-A triblock and multiblock copoly(ester-ether)s consisting of poly(L-Lactide) and poly(oxyethylene-co-oxypropylene) were prepared and characterized. The preparation of the triblock copolymer was done by ring-opening copolymerization of L-lactide with a commercially available telechelic copolyether, Pluronic$\^$TM/(PN) by catalysis of stannous octanoate. The molecular weight and unit composition of the produced copolymers were successfully controlled by changing the L-lactide/PN ratio in feed. However, a high molecular weight copolymer incorporating PN in large amount was not obtained because the molecular weight of the resulting copolymer was limited at a high L-lactide/PN composition. The multiblock copolymer was synthesized by the copolycondensation of oligo(L-lactic acid) prepared by thermal dehydration of L-lactic acid, PN, and dodecanedioic acid as carboxyl/hydroxyl adjusting agent. This polycondensation proceeded by catalysis of stannous oxide to give multiblock copolymers with high molecular weight and wide range of compositions.

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칡전분을 첨가한 칡설기의 재료배합비에 따른 관능적.텍스쳐 특성 (Sensory and textural characteristics of Chicksulgi using varied levels of arrowroot starch and different types of sweeteners)

  • 이효지;정낙원;차경희
    • 한국식품조리과학회지
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    • 제18권3호
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    • pp.372-380
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    • 2002
  • The objective of this study was to invesitigate the sensory and quality characteristics of Chicksulgi containing different ratios of ingredients such as arrowroot starch (10%, 20%, and 30%), sugar, honey, oligo-saccharide and water, by sensory evaluation and mechanical examination. The results of sensory evaluation showed that Chicksulgi containing 10% arrowroot starch had higher scores in overall acceptability, color and flavor preference. In the textural analysis of Chicksulgi, the springiness, cohesiveness, adhesiveness were the highest in the samples with 10% arrowroot starch, and the chewiness, gumminess, and hardness were the highest in the 30%-added samples. The hunter's color L value of Chicksulgi was decreased by the increase of arrowroot starch. The more arrowroot starch was added, the redness of Chicksulgi were increased, and yellowness of Chicksulgi were decreased. The moisture content of Chicksulgi was higher in the samples with 10% arrowroot starch than those with 30%.

Genetic Relationships and Phylogeny of the Asplenium antiquum Makino (Aspleniaceae) and its relative species based on RAPD Analysis

  • Kim, Joo-Hwan;Tea, Kyoung-Hwan
    • Plant Resources
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    • 제5권1호
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    • pp.86-94
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    • 2002
  • This study characterized the genetic variations of 13 populations of Asplenium antiquum and its relative species using randomly amplified polymorphic DNA (RAPD) markers. A total 88 scorable RAPD bands were generated by the 12 random oligo primers and were analyzed by Nei and Li's genetic distance. High genetic variability was detected between A. antiquum and A. nidus, with the range from 0.568 to 0.682. And slightly low genetic variations showed within the populations of same species. Seven populations of A. antiquum showed slight differences (0.000-0.216), and five populations of A. nidus showed similar low genetic variations (0.114 to 0.171). Two individuals from Sup-seom Island which are growing in might be the regenerated one from abroad. A. antiquum were clustered as two groups (Group I, Group II) by UPGMA phenogram. And five populations of A. nidus were clustered as two groups correlated with geographical distribution. The RAPD data was very useful to define the genetic variations and to discuss the phylogenetic relationships among A. antiquum and the related species..

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Pathological and molecular comparisons of five distinct species of pepper-infecting Potyviruses (oral)

  • Yoon, H.I.;Chung, H.M.;Ryu, K.H.
    • 한국식물병리학회:학술대회논문집
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    • 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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    • pp.113.2-114
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    • 2003
  • Five pepper-infecting potyviruses, Pepper mottle virus (PepMoV), Chilli veinal mottle virus (CVMV), Pepper veinal mottle virus (PVMV), Pepper severe mosaic virus (PSMV) and Tobacco each virus (TEV), are known filamentous virus and can be infected pepper crops systemically. To understand pathology and genome information of the five viruses on pepper plants, host reactions and sequences were compared to the 5 viruses. Five potyviruses were inoculated onto some typical cultivars of hot peppers and compared their symptoms, and virus accumulations. A set of degenerate primers for potyviruses were applied to 5 viruses and RT-PCR was performed. RT-PCR products containing partial nuclear inclusion b and coat protein (CP) genes were cloned. Then, oligo dT primer and species-specific primer were redesigned to amplify the C-terminal part of CP and 3' noncoding regions of each viruses. Sequences of the viruses were analyzed and compared to serological relationships among the viruses. The data can be useful for screening of potyviruses in pepper plants and pathogen-derived transgenic pepper plant development.

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