• Molecules and Cells
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• v.39 no.1
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• pp.65-71
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• 2016

A challenge in the redox field is the elucidation of the molecular mechanisms, by which $H_2O_2$ mediates signal transduction in cells. This is relevant since redox pathways are disturbed in some pathologies. The transcription factor OxyR is the $H_2O_2$ sensor in bacteria, whereas Cys-based peroxidases are involved in the perception of this oxidant in eukaryotic cells. Three possible mechanisms may be involved in $H_2O_2$ signaling that are not mutually exclusive. In the simplest pathway, $H_2O_2$ signals through direct oxidation of the signaling protein, such as a phosphatase or a transcription factor. Although signaling proteins are frequently observed in the oxidized state in biological systems, in most cases their direct oxidation by $H_2O_2$ is too slow ($10^1M^{-1}s^{-1}$ range) to outcompete Cys-based peroxidases and glutathione. In some particular cellular compartments (such as vicinity of NADPH oxidases), it is possible that a signaling protein faces extremely high $H_2O_2$ concentrations, making the direct oxidation feasible. Alternatively, high $H_2O_2$ levels can hyperoxidize peroxiredoxins leading to local building up of $H_2O_2$ that then could oxidize a signaling protein (floodgate hypothesis). In a second model, $H_2O_2$ oxidizes Cys-based peroxidases that then through thiol-disulfide reshuffling would transmit the oxidized equivalents to the signaling protein. The third model of signaling is centered on the reducing substrate of Cys-based peroxidases that in most cases is thioredoxin. Is this model, peroxiredoxins would signal by modulating the thioredoxin redox status. More kinetic data is required to allow the identification of the complex network of thiol switches.

• Proceedings of the Korean Magnestics Society Conference
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• 2008.12a
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• pp.217.2-217.2
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• 2008

• BMB Reports
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• v.40 no.5
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• pp.740-748
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• 2007

To gain insight into the structure and function of repressor proteins of bacteriophages of gram-positive bacteria, repressor of temperate Staphylococcus aureus phage ${\phi}11$ was undertaken as a model system here and purified as an N-terminal histidine-tagged variant (His-CI) by affinity chromatography. A ~19 kDa protein copurified with intact His-CI (~ 30 kDa) at low level was resulted most possibly due to partial cleavage at its Ala-Gly site. At ~10 nM and higher concentrations, His-CI forms significant amount of dimers in solution. There are two repressor binding sites in ${\phi}11$ cI-cro intergenic region and binding to two sites occurs possibly by a cooperative manner. Two sites dissected by HincII digestion were designated operators $O_L$ and $O_R$, respectively. Equilibrium binding studies indicate that His-CI binds to $O_R$ with a little more strongly than $O_L$ and binding species is probably dimeric in nature. Interestingly His-CI binding affinity reduces drastically at elevated temperatures ($32-42^{\circ}C$). Both $O_L$ and $O_R$ harbor a nearly identical inverted repeat and studies show that ${\phi}11$ repressor binds to each repeat efficiently. Additional analyses indicate that ${\phi}11$ repressor, like $\lambda$ repressor, harbors an N-terminal domain and a C-terminal domain which are separated by a hinge region. Secondary structure of ${\phi}11$ CI even nearly resembles to that of $\lambda$ phage repressor though they differ at sequence level. The putative N-terminal HTH (helix-turn-helix) motif of ${\phi}11$ repressor belongs to the HTH -XRE-family of proteins and shows significant identity to the HTH motifs of some proteins of evolutionary distant organisms but not to HTH motifs of most S. aureus phage repressors.

• The Journal of Information Systems
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• v.32 no.3
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• pp.165-181
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• 2023

Purpose Digital platforms, together with the innovative technologies of modern society, are accelerating the digital innovation of the entire economy and society. Although social media platforms are gradually integrated into daily life, due to social media overload, users limit their use of the platform for a certain period of time or eventually choose to stop using it. In the context of social media platform, the purpose of this paper is to study the effects of information overload, social overload and system function overload on users' unintentional avoidance behavior, mediated by fatique and dissatisfaction. Design/methodology/approach This study empirically examines the influence of social media overload characteristics on users' unintentional avoidance behavior of platform utilization using the S-O-R framework. Data from 236 Chinese social media users were collected through a questionnaire survey, and the hypotheses were validated by evaluating the research model using the SmartPLS 4.0 program using Partial Least Square (PLS) method. Findings According to the empirical analysis result, based on the S-O-R model, first, it is confirmed that information overload and system feature overload have significant positive(+) effects on fatigue. Second, this study finds that information overload, social overload and fatigue have significant positive(+) effects on dissatisfaction. Thirdly, fatigue and dissatisfaction have significant positive(+) effects on unintentional avoidance. In addition, social overload has no significant effect on fatigue, while system feature overload has no significant effect on dissatisfaction.

• Korean Journal of Crystallography
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• v.18 no.1_2
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• pp.1-6
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• 2007

An Organometallic compound, $C_{16}H_{35}B_{10}IrS_2Si$, was synthesized from o-carborane, $Cp^*Ir(S_2C_2B{10}H_{10})$, and $Me_3SiCHN_2$. The molecular structure of this complex has been determined by X-ray diffraction. Crystallographic data : monoclinic, space group $P2_1/n$, $a=10.1986(12)\;{\AA}$, $b=14.834(5)\;{\AA}$, $c=17.139\;{\AA}$, ${\beta}=92.24(2)^{\circ}$, Z=4, $V=2591.0(14)\;{\AA}^3$. The structure was solved by direct methods and refined by full-matrix leat-squares methods to give a model with a reliability factor R=0.053 for 5080 reflections.

• Applied Microscopy
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• v.49
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• pp.1.1-1.10
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• 2019

Joints of three-dimensional (3D) rutile-type (r) tin dioxide ($SnO_2$) nanowire networks, produced by the flame transport synthesis (FTS), are formed by coherent twin boundaries at $(101)^r$ serving for the interpenetration of the nanowires. Transmission electron microscopy (TEM) methods, i.e. high resolution and (precession) electron diffraction (PED), were utilized to collect information of the atomic interface structure along the edge-on zone axes $[010]^r$, $[111]^r$ and superposition directions $[001]^r$, $[101]^r$. A model of the twin boundary is generated by a supercell approach, serving as base for simulations of all given real and reciprocal space data as for the elaboration of three-dimensional, i.e. relrod and higher order Laue zones (HOLZ), contributions to the intensity distribution of PED patterns. Confirmed by the comparison of simulated and experimental findings, details of the structural distortion at the twin boundary can be demonstrated.

• Journal of Food Hygiene and Safety
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• v.24 no.3
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• pp.232-237
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• 2009

This study was conducted to develop a model for describing the effect of storage temperature (4, 10, 15, 20, 25, 30 and $35^{\circ}C$) on the growth of Escherichia coli O157 : H7 in ready-to-eat (RTE) lettuce treated with or without (control) alkaline electrolyzed water (AIEW). The growth curves were well fitted with the Gompertz equation, which was used to determine the specific growth rate (SGR) and lag time (LT) of E. coli O157 : H7 ($R^2$ = 0.994). Results showed that the obtained SGR and LT were dependent on the storage temperature. The growth rate increased with increasing temperature from 4 to $35^{\circ}C$. The square root models were used to evaluate the effect of storage temperature on the growth of E. coli O157 : H7 in lettuce samples treated without or with AIEW. The coefficient of determination ($R^2$), adjusted determination coefficient ($R^2_{Adj}$), and mean square error (MSE) were employed to validate the established models. It showed that $R^2$ and $R^_{Adj}$ were close to 1 (> 0.93), and MSE calculated from models of untreated and treated lettuce were 0.031 and 0.025, respectively. The results demonstrated that the overall predictions of the growth of E. coli O157: H7 agreed with the observed data.

• Journal of Korean Society of Transportation
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• v.33 no.6
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• pp.575-583
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• 2015

The Korea Transport Institute (KOTI) builds the origin and destination(O-D) trip data with relatively smaller zone size such as Eup, Myeon, Dong administration unit districts in metropolitan area. Otherwise, O-D trip data was built by bigger size of traffic analysis zone(TAZ) such as Si, Gun, Gu administration unit districts for rural area. In some cases, it is needed to divide a zone into several sub-zones for rural area in order to analyze travel distribution pattern in detail for a certain highway and rail project. The study suggested a method to estimate O-D trips for sub-zones in the larger-size zones in rural area. Two different distribution models, direct demand model and gravity model, were calibrated for sub-zone's intra-zonal O-D trip pattern with metropolitan area O-D data which has smaller zone-size (sub-zone) data categorized by low, middle and high population density. The calibration results were compared between the two models. The gravity model with impedance function of power functional form was selected with better explanation for all groups in the metropolitan area. The adjusted $R^2$ was 0.7426, 0.6456 and 0.7194 for low, middle and high population density group, respectively. The suggested O-D trip estimating method is expected to produce enhanced trip patterns with sub-divided small zones.

• Journal of Korean Society of Transportation
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• v.31 no.2
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• pp.60-68
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• 2013

This study developed plural gravity models and their application method in order to increase the accuracy of trip distribution estimation. The developed method initially involves utilizing the coefficient of determination ($R^2$) to set the target level. Afterwards, the gravity model is created, and if the gravity model's coefficient of determination is satisfactory in regards to the target level, the model creation is complete and future trip distribution estimation is calculated. If the coefficient of determination is not on par with the target level, the zone pair with the largest standardized residual is removed from the model until the target level is obtained. In respect to the model, the removed zone pairs are divided into positive(+) and negative(-) sides. In each of these sides, gravity models are made until the target level is reached. If there are no more zone pairs to remove, the model making process concludes, and future trip distribution estimation is calculated. The newly developed plural gravity model and application method was adopted for 42 zone pairs as a case study. The existing method of utilizing only one gravity model exhibited a coefficient of determination value ($R^2$) of 51.3%, however, the newly developed method produced three gravity models, and exhibited a coefficient of determination value ($R^2$) of over 90%. Also, the accuracy of the future trip distribution estimation was found to be higher than the existing method.

• Journal of KIISE:Computer Systems and Theory
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• v.33 no.7
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• pp.448-453
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• 2006

We extend a parallel depth-first search (DFS) algorithm for planar graphs to deal with (non-planar) solid grid graphs, a subclass of non-planar grid graphs. The proposed algorithm takes time O(log n) with $O(n/sqrt{log\;n})$ processors in Priority PRAM model. In our knowledge, this is the first deterministic NC algorithm for a non-planar graph class.