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Application of Computer-coupled Mass Spectrometer for Continuous On-line Monitoring of Cell Growth and Growth Rate (세포증식과 증식속도의 On-line Monitoring을 위한 Computer- coupled Mass Spectrometer의 응용)

  • 남수완;최춘순;김정회
    • Microbiology and Biotechnology Letters
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    • v.17 no.3
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    • pp.241-246
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    • 1989
  • Continuous on-line monitoring of cell concentration and growth rate in aerobic batch fermentation process was carried out by analyzing the exhaust gas composition of tormentor with a quadrupole mass spectrometer. From the mass spectrometric analyses of major gaseous components, i.e. $N_2$, $O_2$, $CO_2$ and $H_2O$, and the material balance equations for oxygen and carbon dioxide, oxygen uptake rate (OUR) rind carbon dioxide evolution rate (CER) were instantaneously calculated using a computer (16-bit IBM PC-AT) interfaced to a quadrupole mass spectrometer. The calculated OUR and CER data were used for the estimation of cell concentration and growth rate of Candida utilis during batch culture. It was found that the cell concentration could be satisfactorily estimated from the data of OUR arid CER during the culture and this method could be successfully und for the continuous monitoring of cell growth and growth rate.

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Cytogenetic Analysis of Bupleurum falcatum L. Cultivated in Korea (한국 재배종 시호의 세포유전학적 분석)

  • Chung, Sung-Hyun;Bang, Jae-Wook;Choi, Hae-Woon
    • Korean Journal of Medicinal Crop Science
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    • v.3 no.1
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    • pp.61-65
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    • 1995
  • Karyotype analysis was carried out in four lines of Bupleurum falcatum L. cultivated in Korea and SDS-PAGE was applied to determine the seed protein profiles among the lines. Chromosomes were classified into two groups, large and small ones. Two kinds of karyotype, 2n=20 and 2n=26, were identified. Chromosome 1 of 2n=20 were all submedian, while that of 2n=26 were median. Chromosomes 2, 3 and 5 of 2n=20 showed polymorphism in size and arm-ratio. Chromosome 2 was submedian, while others were median in the line of 2n=26. Karyotypcs of cultivars native of Korea were similiar each other, while those introduced from Japan showed different patterns. In SDS PAGE gels, qualitative difference s in high molecular weight proteins, more than 45KD, were detected among the lines. The numbers of specific band were three in lines of 2n=20 and two in 2n=26.

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Improved Impedance Matching of Dual-Frequency Microstrip Printed-Dipole Antenna with Conductor Back

  • Tangjitjesada, M.;Anantrasirichai, N.;Wakabayashi, T.
    • 제어로봇시스템학회:학술대회논문집
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    • 2003.10a
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    • pp.1668-1671
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    • 2003
  • A novel dual-frequency microstrip printed-dipole antenna operating at 5 GHz and 10 GHz is presented. This antenna is designed for wireless and mobile communication. The balance step coplanar strip is used to be a transmission line at the center of dipole with matching impedance at 50 ohm. Using the conductor strip align on the other side of antenna and adjust the width of step coplanar strip line to improved input impedance matching. By modification for matching impedance of dual frequency antenna are not affected to the radiation patterns. The Finite Difference Time Domain (FDTD) technique is applying to analyze the basic characteristic properties such as $S_{11}$ , input impedance , VSWR and radiation patterns. And these parameters are discussed. The analyze problem space are $51{\times}197{\times}175$ cells and cell dimension are ${\Delta}x=0.3\;mm$ and ${\Delta}y={\Delta}z=0.15\;mm$.

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Earthworms from Chejoo-do Island, Korea (제주도산 육서 빈모류의 분류학적 연구)

  • Song, Min-Ja;Paik, Kap-Yong
    • The Korean Journal of Zoology
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    • v.13 no.1
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    • pp.9-14
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    • 1970
  • 제주도산 육서 빈모류의 분류학적 연구는 Kobayashi, S. (小林新二郞)에 依하여 1937년에 Drawida anchingiana, Pheretima kanrazana, Ph. masatakae, Ph. quelparta 등의 4종이 보고 되었고, 다시 1938년에 Dr. japonica, Ph. carnosa, Ph. hupeiensis가 보고 되었을 뿐이다. 저자들은 1966년 및 1969년에 채집된 제주도산 지렁이 총 1045개체를 정리한 결과 다음 2과 2속 11종을 얻었기에 이에 보고 하는 바이다. 제주도 기지종 : Pheretima carnosa, Ph. hupeiensis, Ph. kanrazana, Ph. masatakae, Ph. quelparta. 제주도 미기록종 : Allolobophora caliginosa trapezoides, Ph. agrestsi, Ph. heteropoda. Ph. phaselus var. kamitai, Ph. soulensis. 신종 : Ph. seungpanesis n. sp. 신종 pheretima seungpanensis의 체장은 $105 \\sim 120mm$, 체폭은 $5 \\sim 6mm$, 체절수는 $78 \\sim 98$개 spermathecal pore는 2쌍으로서 첫째쌍은 VI체절의 setal line 뒷쪽에서 이 선과 인접해 있으며 두째쌍은 setal line의 앞쪽에 intersegmental furrow에 가까운 부분에서 개구한다. 본종은 spermathecal pore의 위치가 이속의 다른 종에서 보는것 처럼 intersegmental furrow에 개구하지 않는점이 특별하다. 그 뿐 아니라 male pore region의 모양에 있어서도 모든 기지종과 뚜렷이 구별된다.

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High Resolution Linear Graphs : Graphical Aids for Designing Off-Line Process Control)

  • Lee, Sang-Heon
    • Journal of the military operations research society of Korea
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    • v.27 no.1
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    • pp.73-88
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    • 2001
  • Designing high quality products and processes at a low cost is central technological and economic challenge to the engineer. The combination of engineering concepts and statistical implementations offered by Taguchi\`s off-line design technique has proven t be invaluable. By examining some deficiencies in designs from the Taguchi\`s highly fractional, orthogonal main effect plan based on orthogonal arrays, alternative method is proposed. The maximum resolution or the minimum aberration criterion is commonly used for selecting 2$^{n-m}$ fractional designs. We present new high resolution (low aberration) linear graphs to simplify the complexity of selecting designs with desirable statistical properties. The new linear graphs approach shows a substantial improvement over Taguchi\`s linear graphs and other related graphical methods for planning experiment. The new set of linear graphs will allow the experimenter to maintain the simple approach suggested by Taguchi while obtaining the best statistical properties of the resulting design such as minimum aberration as a by-product without dependency on complicated computational algorithm or additional statistical training.g.

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HEVA: Cooperative Localization using a Combined Non-Parametric Belief Propagation and Variational Message Passing Approach

  • Oikonomou-Filandras, Panagiotis-Agis;Wong, Kai-Kit
    • Journal of Communications and Networks
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    • v.18 no.3
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    • pp.397-410
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    • 2016
  • This paper proposes a novel cooperative localization method for distributed wireless networks in 3-dimensional (3D) global positioning system (GPS) denied environments. The proposed method, which is referred to as hybrid ellipsoidal variational algorithm (HEVA), combines the use of non-parametric belief propagation (NBP) and variational Bayes (VB) to benefit from both the use of the rich information in NBP and compact communication size of a parametric form. InHEVA, two novel filters are also employed. The first one mitigates non-line-of-sight (NLoS) time-of-arrival (ToA) messages, permitting it to work well in high noise environments with NLoS bias while the second one decreases the number of calculations. Simulation results illustrate that HEVA significantly outperforms traditional NBP methods in localization while requires only 50% of their complexity. The superiority of VB over other clustering techniques is also shown.