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Acoustic analysis on the shape of gill-net in the current (자망의 수중형상에 대한 음향학적 해석)

  • Han, Jin-Seok;Shin, Hyeon-Ok
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.43 no.2
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    • pp.116-125
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    • 2007
  • An experiment to acoustically analyze the shape of gill-net in the current was conducted in Jaran Bay, Gosung, Korea on the 9th to 10th September(spring tide) and 28th to 29th September(neap tide) 2006. It was measured by a 3D underwater positioning system with a radio-acoustic linked positioning buoys. Six of 7 acoustic transmitters used in the experiment were attached on the float line of the gill-net and the other was fixed on the sea bed. During spring tide, the maximum movement of the gill-net was 27.0m(22:00) in the west(4.4cm/s, $311.9^{\circ}$) and 20.6m(04:00) in the east(3.9cm/s, $66.5^{\circ}$). The maximum extension of the gill-net(the distance between P1 and P6) was 119.8m(21:00, 11.6cm/s, $321.9^{\circ}$) and the minimum was 109.9m(23:00, 16.1cm/s, $88.5^{\circ}$). During neap tide, the maximum movement was 38.0m(20:00) in the east(9.6cm/s, $278.2^{\circ}$) and 11.0m(12:00) in the west(1.9cm/s, $232.1^{\circ}$). The maximum extension was 99.6m(14:00, 12.5cm/s, $94.7^{\circ}$) and the minimum was 85.0m(06:00, 9.0cm/s, $265.8^{\circ}$). During spring tide, the maximum height of the gill-net from the sea bed was 3.7m(02:00, 7.4cm/s, $151.6^{\circ}$) and the minimum was produced the three times as 1.5m. At that time, the current speed and direction was 17.9cm/s and $85.3^{\circ}$(23:30), 16.1cm/s and $249.4^{\circ}$(05:00), and 13.7cm/s and $291.4^{\circ}$(06:30), respectively. During neap tide, the maximum height was 3.6m(12:30, 2.1cm/s, $242.3^{\circ}$) and the minimum was 1.5m(14:00, 12.5cm/s, $94.7^{\circ}$).

Study on Environmental Hazards of Alternatives for PFOS (PFOS 대체물질의 환경유해성에 관한 연구)

  • Choi, Bong-In;Chung, Seon-Yong;Na, Suk-Hyun;Shin, Dong-Soo;Ryu, Byung-Taek
    • Journal of Korean Society of Environmental Engineers
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    • v.38 no.6
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    • pp.317-322
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    • 2016
  • While PFOS sodium salt ($C_8F_{17}SO_3Na$) was not degraded by microorganisms for 28 days, the 4 alternatives were biodegraded at the rates of 21.6% for $C_{25}F_{17}H_{32}S_3O_{13}Na_3$, 20.5% for $C_{15}F_9H_{21}S_2O_8Na_2$, 15.8% for $C_{23}F_{18}H_{28}S_2O_8Na_2$ and 6.4% for $C_{17}F_9H_{25}S_2O_8Na_2$, respectively. The acute toxicity test using Daphnia magna was conducted for 48 hours, the half effective concentration ($EC_{50}$) of PFOS sodium salt ($C_8F_{17}SO_3Na$) was evaluated in 54.5 mg/L. While the 4 alternatives did not show any effect at 500.0 mg/L. The surface tension of the PFOS salt ($C_8F_{17}SO_3Na$) is 46.2 mN/m at a concentration of 500.0 mg/L. While the surface tension of the 4 alternatives was found to be superior to PFOS sodium salt ($C_8F_{17}SO_3Na$). The surface tension of $C_{23}F_{18}H_{28}S_2O_8Na_2$ (20.9 mN/m) has the lowest, followed by $C_{15}F_9H_{21}S_2O_8Na_2$ (23.4 mN/m), $C_{17}F_9H_{25}S_2O_8Na_2$ (27.3 mN/m), $C_{25}F_{17}H_{32}S_3O_{13}Na_3$ (28.2 mN/m). The four kinds of alternatives ($C_{15}F_9H_{21}S_2O_8Na_2$, $C_{17}F_9H_{25}S_2O_8Na_2$, $C_{23}F_{18}H_{28}S_2O_8Na_2$, $C_{25}F_{17}H_{32}S_3O_{13}Na_3$) were found to be superior to PFOS sodium salt ($C_8F_{17}SO_3Na$) in terms of biodegradation, Daphnia sp. acute toxicity and surface tension, and thus they were considered applicable as PFOS alternatives. Especially biodegradation rate of $C_{15}F_9H_{21}S_2O_8Na_2$, $C_{23}F_{18}H_{28}S_2O_8Na_2$ and $C_{25}F_{17}H_{32}S_3O_{13}Na_3$ was relatively high as 15.8~21.6%, and Daphnia sp. acute toxicity and surface tension were considerably superior (surface tension 39~55%) to PFOS sodium salt. Therefore, these alternatives are considered to be available as an alternative of PFOS.

STATISTICAL STUDY ON THE CARABELLI'S TUBERCLE OF UPPER FIRST MOLAR IN KOREAN CHILDREN (한국인아동에 있어서 상악제일대구치의 Carabelli's 결절에 관한 통계학적연구)

  • Kim, Sung-Ryong
    • The Journal of the Korean dental association
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    • v.10 no.11
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    • pp.731-733
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    • 1972
  • Occurence of different forms of Carabelli's cusp on the maxillary first permanent molar was studied in 282 Korean children. The results were as follows; 1. The Carabelli's cusp was absent in 36.2% of the teeth studied. 2. The percentages of various form appeared Carabelli's tubercle were as follows; a. Pronounced tubercle............9.9% b. Slight tubercle............24.5% c. Groove............25.5% d. Pit............3.9%

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PZ-peptidase activities in Streptococcus sanguis and other oral bacteria (Streptococcus sanguis와 여타 구강세균이 생산하는 PZ-peptidase 활성)

  • 최선진
    • Korean Journal of Microbiology
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    • v.21 no.3
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    • pp.143-148
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    • 1983
  • The occurrence of PZ-peptidase in Streptococcus sanguis and other oral bacteria was investigated utilizing washed whole cells as the enzyme source and PZ-pentapeptide as its substrate. Under the culture conditions employed in the present study. Streptococcus sanguis strains, fresh isolates as well as laboratory strains, produced a broad range of the enzyme activity (0.5-7.9 unit/mg protein). The strains of both Streptococcus mutans and Lactobacilli showed low levels of activity (0-0.5 unit/mg protein for S. mutans). As compared with the enzyme activities of other bacteria, a moderate range of activity was produced by the strains of Strptococcus mitis nad Strptoccus salivarius. Actinomyces strains, like those of S. sanguis, produced a varying amount of activity (0-9.8 unit/ mg protein). A possible involvement of the oral bacterial PZ-peptidase in the metabolism of human saliva proteins is discussed.

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Isolation Frequency of Staphylococcus species from Clinical Materials (임상검체에서 포도알균의 분리 빈도)

  • Shin, Hyun-Sung;Park, Youn-Bo
    • Korean Journal of Clinical Laboratory Science
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    • v.39 no.2
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    • pp.76-85
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    • 2007
  • The total number of clinical materials collected from Dec 1, 2005 to Nov 30, 2006 in C. hospital was 63,133. Among these materials, the isolation of Staphylococcus spp. was 5,252 strains. The proportions of Staphylococcus species infection were as follows ; 46.7% in S. aureus, 39.3% in S. epidermidis, 4.7% in S. hominis, 3.8% in S. haemolyticus, 2.9% in S. capitis and 2.5% in others. Seventeen strains of Staphylococcus species were identified with an isolation range of 0.02-46.7%. The isolation ratios of Staphylococcus species were 7.8% (2,134/27,362) in female, 8.7% (3,118/35,807) in male and 8.3% (5,252/63,133) in both. The age-related frequencies of Staphylococcus species were 22.1% in the 60-69 year group, 19.3% in the 70-79 year group and 17.1% in the 50-59 year group. The isolation ratio of the 0-49 year group to the 50-over 80 year group were 1.74 times in female, 1.92 in male and 1.85 in both. The isolation frequency of Staphylococcus spp. was at its highest in April (9.9%) and at its lowest in February (6.8%) according to monthly analysis. According to seasonal variation, the isolation frequencies of Staphylococcus spp. were 27.0% in Summer, 25.9% in Spring, 23.9% in Fall and 23.2% in Winter. The clinical departments showing over 9% isolation rate against Staphylococcus spp. were critical care medicine (12.9%), department of pediatrics (11.6%), department of urology (9.8%) and department of neuro-surgery (9.1%). On the other hand, the lowest number was observed in the department of family medicine. The clinical material showing over 15% isolation rate against Staphylococcus spp. were 32.1% in urine (random urine and catheterized urine), 20.6% in blood and 16.0% in sputum.

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Increased Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 Levels in the Cerebrospinal Fluid from Children with Aseptic Meningitis (무균성 뇌수막염 소아에서 뇌척수액내 Matrix Metalloproteinase(MMP)-9과 Tissue Inhibitor of Metalloproteinase(TIMP)-1의 증가)

  • Yang, Ju Hee;Park, Min Hyuk;Shim, Jung-Yeon;Jung, Hye Lim;Park, Moon Soo;Keum, Dong Hyuck
    • Clinical and Experimental Pediatrics
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    • v.46 no.6
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    • pp.548-553
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    • 2003
  • Purpose : Matrix metalloproteinase(MMP)-9 is known to breakdown the blood-brain barrier by degrading the extracellular matrix of the subendothelial basement membrane in meningitis. Tissue inhibitor of metalloproteinase(TIMP)-1, a known inhibitor of MMP-9, has been postulated to inhibit the proteolytic activity of MMP-9 by bindng to MMP-9, but their interaction has not been fully understood yet. So far, there have been some reports on the relationship of MMP-9 and TIMP-1 in bacterial meningitis, but few reports in viral meningitis. Furthermore, there has been no report on this in Korea. We investigated the concentrations of MMP-9 and TIMP-1 in cerebrospinal fluid (CSF) and serum of patients with viral meningitis and control subjects, and evaluated their relationship with other clinical parameters of meningitis. Methods : CSF and blood were obtained from 25 subjects with viral meningitis and 14 control subjects. After centrifugation, supernatants were stored at $-20^{\circ}C$ and we assayed concentrations of MMP-9 and TIMP-1 by the sandwich ELISA method. Results : Concentrations of CSF MMP-9 and TIMP-1 were significantly elevated in patients with viral meningitis, when compared with those in control subjects. Their serum levels showed no differences between the two groups. MMP-9 levels were closely correlated with TIMP-1 levels in the CSF($r_s=0.42$, P<0.05). CSF MMP-9/TIMP-1 ratios were significantly higher in patients with viral meningitis than those in the control subjects(P<0.05). Both CSF MMP-9 and TIMP-1 levels positively correlated with CSF total leukocyte counts($r_s=0.43$, P<0.05, $r_s=0.48$, P<0.05). TIMP-1 levels positively correlated with total protein concentrations in the CSF($r_s=0.43$, P<0.05). Conclusion : MMP-9 and TIMP-1 may play an important role in the breakdown and maintenance of BBB in viral meningitis, respectively.

Sperm-Associated Antigen 9 is a Promising marker for Early Diagnosis of Endometrial Cancer

  • Baser, Eralp;Togrul, Cihan;Ozgu, Emre;Ayhan, Sevgi;Caglar, Mete;Erkaya, Salim;Gungor, Tayfun
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.12
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    • pp.7635-7638
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    • 2013
  • Background: Sperm-associated antigen 9 (SPAG9) has been recently proposed as a novel biomarker for early diagnosis of several human tumors, including ovarian, cervical and breast cancers. Its clinical value remains to be clarified for endometrial cancer (EC). In this study, we investigated the utility of serum SPAG9 levels in diagnosis of EC and its association with important clinicopathological parameters. Materials and Methods: This cross-sectional study was performed at a tertiary women's referral center in Ankara, Turkey. Preoperative serum samples were collected from patients surgically treated for endometrial cancer between June 2012-April 2013. Similar aged women with a biopsy proven benign endometrium were used as controls. Serum SPAG9 levels were measured with an enzyme-linked immunosorbent assay (ELISA) method and assessed for links with clinicopathological factors. Receiver operating characteristic (ROC) curve analysis was performed to assess power of SPAG9 levels for EC prediction. P values less than 0.05 were considered statistically significant. Results: A total of 63 women with EC and 27 with benign endometrium were included in the study. Mean age in the EC group was $58.7{\pm}1.1$. Median SPAG9 levels in the EC and control groups were 18.3 (range, 12.7-53.8) and 14.1 (range, 4.3-65.3), respectively (p<0.001). A cut-off value of 17 ng/ml for SPAG9 predicted presence of malignant endometrium with 74% sensitivity and 83% specificity [Area under curve (AUC)=0.82, p<0.001]. SPAG9 levels did not demonstrate any significant association with histological type, FIGO stage, tumor grade, size, myometrial invasion, lymphovascular space invasion, cervical involvement, adnexal involvement, peritoneal cytology or lymph node status (all p>0.05). Conclusions: Testing for SPAG9 may be useful for early detection of EC in asymptomatic high-risk women. Its role in post-treatment follow-up and early detection of recurrence should be assessed in future trials.

The Developmental Characterization of the Sericin jam(Bombyx mori), a Mutant in the Synthesis and Secretion of Fibroin (세리신잠(Nd-s. $Nd^H$의 발육 특성)

  • 김수연;손해룡
    • Journal of Sericultural and Entomological Science
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    • v.45 no.1
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    • pp.6-9
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    • 2003
  • This experiment was done to know developmental characteristics of Sericin jam. Incubation periods were 10 day 2 hr, and 10 day 1 hr. 11 day 1 hr for Nd-s jam, N $d^{H}$ jam, and Baegok jam, respectively. Hatching rates were 83.9, 83.3 and 96.0% for Nd-s jam, N $d^{H}$ jam, and Baegok jam. Larval periods were, 20 days 1 hr for Nd-s jam, 20 days 5 hrs for N $d^{H}$ jam, and 22 days 12 hrs for Baegok jam. Death rate of larvae was highest in N $d^{H}$ iam, followed by Nd-s jam and Baegok jam. Pupation rate was highest in Baegok Jam followed by Nd-s jam and that of N $d^{H}$ jam was the lowest among the three. Cocoon weight was 1.39, 1.08, and 2.01 g for Nd-s jam, N $d^{H}$ jam, and Baegok jam, respectively. Shell weight were 13, 3, and 48 cg for Nd-s jam, N $d^{H}$ jam, and Baegok jam. Cocoon shell ratios were 9.0% for Nd-s jam, 2.8% for N $d^{H}$ jam and 23.9% for Baegok jam. Cocoon sizes were 30.6${\times}$15.8 mm for Nd-s jam, 24.7${\times}$14.9 mm for N $d^{H}$ jam and 35.8 ${\times}$ 20.5 mm(1${\times}$w) for Baegok jam.w) for Baegok jam.

Species-Specific Cleavage by RNase E-Like Enzymes in 5S rRNA Maturation

  • RYOU SANG-MI;KIM JONG-MYUNG;YEOM JI-HYUN;KIM HYUN-LI;GO HA-YOUNG;SHIN EUN-KYOUNG;LEE KANGSEOK
    • Journal of Microbiology and Biotechnology
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    • v.15 no.5
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    • pp.1100-1105
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    • 2005
  • Previous work has identified a Streptomyces coelicolor gene, rns, encoding a 140 kDa protein (RNase ES) that exhibits the endoribonucleolytic cleavage specificity characteristic of RNase E and confers viability on and allows the propagation of E. coli cells lacking RNase E. Here, we identify a putative S. coelicolor 9S rRNA sequence and sites cleaved by RNase ES. The cleavage of the S. coelicolor 9S rRNA transcript by RNase ES resulted in a 5S rRNA precursor (p5S) that had four and two additional nucleotides at the 5' end and 3' ends of the mature 5S rRNA, respectively. However, despite the similarities between RNase E and RNase ES, these enzymes could accurately process 9S rRNA from just their own bacteria, indicating that these ancient enzymes and the rRNA segments that they attack appear to have co-evolved.

Microbial Control of Fly Maggots with Entomopathogenic Nematodes and Fungus in Outhouses of Farmhouses (곤충병원선충과 곰팡이를 이용한 농가화장실 파리의 미생물적 방제)

  • 추호렬;김형환;이동운;박영도
    • Korean journal of applied entomology
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    • v.35 no.1
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    • pp.80-84
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    • 1996
  • Infectivity of entomopathogenic nematodes and fungus was evaluated against fly larvae in the laboratory and outhouses. Mortalities of Muscina stabulans larvae were 96.7f 2.8% in Steinernema glaseri Dongrae strain, 90.0+0.0% in S. carpocapsae All strain, 86.7f 2.7% in Heterorhabditis bacteriophora Hamyang strain, and 70.0+9.4% in S. carpocapsae Pocheon strain on the filter paper. When 260, 000 nem\ulcornertodes were sprayed into the outhouses, H. bacterwphora Hamyang strain killed 100%, S. glaseri Dongrae strain killed 76.9+3.9%, and S. carpocapsae Pocheon strain killed 58.5+6.1% of maggots. When 130, 000 nematodes and 7.0X lo9 cfu of entomopathogenic fungus, Beauveria brongniartii were sprayed alone or combined into outhouses, mortalities of maggots were 73.6+0.1% in B. brongniartii alone, 77.8+3.9% in S. carpocapsae Pocheon strain plus B. brongniartii, and 77.7f 5.1% in H. bacteriophora Hamyang strain plus B. brongniartii. Entomopathogenic nematodes and fungus were potential biological control agents in this study.

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