• Title/Summary/Keyword: S-47

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Cloning and Nucleotide Sequence Analysis of xylC Gene Encoding 5C-2HMS Dehydrogenase from Pseudomonas sp. S-47. (Pseudomonas sp. S-47로부터 5-Chloro-2-Hydroxymuconic Semialdehyde Dehydrogenase를 암호화하는 xylG 유전자의 클로닝 및 염기서열 분석)

  • Park, Song-Yi;Lee, Dong-Hoon;Kim, Young-Soo;Lee, Kyung;Kim, Chi-Kyung
    • Microbiology and Biotechnology Letters
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    • v.30 no.1
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    • pp.8-14
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    • 2002

  • Pseudomonas sp. S-47 is capable of degrading 4-chlorobenzoate to produce 5-chloro-2-hydroxymuconic semialdehyde (5C-2HMS) by the enzymes encoding by xylXYZLTE cluster. In this study, the resulting 5C-2HMS was confirmed to be transformed to 5-chloro-2-hydroxymuconic acid (5C-2HMA) by 5C-2HMS dehydrogenase. The xylG gene encoding 5C-2HMS dehydrogenase was cloned from the chromosomal DNA of strain S-47. The nucleotide sequence of xylG showed to be composed of 1,600 base pairs with ATG initiation and TGA termination codons. A deduced amino acid sequence of the 5C-2HMS dehydrogenase (XylG) exhibited 98％, 93％, and 89％ identity with those of the dehydrogenases from P. putida mt-2, P. putida G7, and Pseudomonas sp. CF600, respectively.

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Vending Culture

  • Korea Vending Machine Manufacturers Association
    • Vending industry
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    • v.6 no.3 s.18
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    • pp.47-47
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    • 2006

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