• BMB Reports
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• v.35 no.4
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• pp.432-436
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• 2002

Pseudomonas sp. S-47 is capable of degrading catechol and 4-chlorocatechol via the meta-cleavage pathway. XyITE products catalyze the dioxygenation of the aromatics. The sylT of the strain S-47 is located just upstream of the xylE gene. XylT of the strain S-47 is located just upstream of the xylE gene. XyIT is typical chloroplast-type ferredoxin, which is characterized by 4 cystein residues that are located at positions 41, 46, 49, and 81. The chloroplast-type ferredoxin of Pseudomonas sp. S-47 exhibited a 98% identity with that of P. putida mt-2(TOL plasmid) in the amino acid sequence, but only about a 40 to 60% identity with the corresponding enzymes from other organisms. We constructed two recombinant plasmids (pRES1 containing xylTE and pRES101 containing xylE without xylT) in order to examine the function of XyIT for the reactivation of the catechol 2,3-dioxygenase (XyIE) that is oxidized with hydrogen peroxide was recovered in the catechol 2,3-dioxygenase (C23O) activity about 4 mimutes after incubation, but the pRES101 showed no recovery. That means that the typical chloroplast-type ferredoxin (XyIT) of Pseudomonas sp. S-47 is involved in the reactivation of the oxidized C23O in the dioxygenolytic cleavage of aromatic compounds.

• The Bulletin of The Korean Astronomical Society
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• v.31 no.2
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• pp.47.2-47.2
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• 2006

• Transactions of the Korean Society of Mechanical Engineers B
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• v.37 no.10
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• pp.933-940
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• 2013

In this study, we evaluated the heat release performance of two extruded-type and two swaged-type heat sinks used in an inverter for solar power generation. The number of fins and heat transfer areas of the two swaged-type heat sinks, namely S-62 and S-98, are 62 and 98 and $2.8m^2$ and $5.3m^2$, respectively. Those for the two extruded-type heat sinks, namely, E-38 and E-47, are 38 and 47 and $1.8m^2$ and $1.9m^2$, respectively. The heat release fractions of S-62 and S-98 were measured as 82.7 % and 86.3 %, respectively. Those of E-38 and E47 were measured to be 79.6 % and 81.6 %, respectively. In this experiment, despite the mass flow rates of air entering the heat sinks being almost the same, the heat release fractions increased with heat transfer area. Furthermore, despite S-62's heat transfer area being 47.4 % higher than that of E-47, its heat release fraction was higher by only 1.3 %. We believe that this indicates the better heat transfer property of the extruded-type heat sink. S-98's heat release is only 4.4 % higher than that of S-62, but its heat transfer area is 89.3 % higher; this suggests that its heat transfer area need to be optimized.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.16 no.6
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• pp.533-539
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• 2023

This study aimed to analyze patients hospitalized for injuries who fell using the data from 2020 to 2021 at institution A located in Gangneung-si, Gangwon-do, using codes for causes of injury. After classifying 20 codes from W00 to W19, which are external cause codes for fall patients, the most frequently occurring W18, W01, W10, and W13 were analyzed. The external cause of injury code W18 was other falls on the same plane, with the highest frequency of S72 and Z47, S72 being a fracture of the femur, and Z47 being orthopedic follow-up treatment. The external injury code W01 was determined to be a fall on the same plane due to slipping, tripping, and tripping, and like W18, S72, a fracture of the femur, and Z47, orthopedic follow-up treatment, were frequently reported. In W10, intracranial injuries such as concussion and epidural hemorrhage due to a fall on the stairs, S06, were common. Lastly, in W13, 91% of cases occurred in people in their 40s to 70s due to falls from buildings or structures, confirming that they occur frequently in middle-aged people, Z47 had the most frequent orthopedic follow-up treatment, and S72 had a fracture of the femur. It was found to be the second most common. In this way, the frequency of falling patients was analyzed, and the age and main diagnosis code at which most falls occurred were analyzed.

• Korean Journal of Environmental Biology
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• v.36 no.3
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• pp.385-391
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• 2018

Toxic assessment of PBDEs (BDE-47, BDE-209) has carried out using the rates of population growth in marine phytoplankton, Skeletonema costatum. The population growth rate (r) in S. costatum was determined after 96 hours of exposure to BDE-47 (2,2'4,4'-Tetrabromodiphenyl ether) and BDE-209 (2,2', 4,4'-Decabromodiphenyl ether). It was observed that r-value in the controls (absence PBDEs) was greater than 0.04 and further a decrease was observed in a dose-dependent manner. BDE-47 reduced population growth rate in a dose-dependent manner and a significant reduction occurred at a concentration greater than $0.31mgL^{-1}$, but BDE-209 had no effect on population growth rate even at concentrations greater than $125mgL^{-1}$. The population growth rate in the presence of BDE-47 and BDE-209, $EC_{50}$ values were $0.55mgL^{-1}$ and >$125mgL^{-1}$, and NOEC values were $0.16mgL^{-1}$ and >$125mgL^{-1}$, respectively. Therefore, BDE-47 is considered to be about over 80 times more harmful than BDE-209. In this study, the ecotoxic assessment based on population growth rate in S. costatum can be used as a baseline data for establishment of environment standard quality of BDE-47 and BDE-209 in the marine environment.

• Journal of Life Science
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• v.19 no.12
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• pp.1724-1730
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• 2009

The mannanase-producing bacteria, designated CS47, was isolated from the fresh feces of three horses (from a farm in Jinju National University). The isolate CS47 was facultatively anaerobic and grew at temperatures ranging from $20^{\circ}C$ to $50^{\circ}C$ with an optimal temperature of $38^{\circ}C$. The DNA G+C content of the isolate CS47 was 44 mlo%. The major fatty acids were anteiso-15:0 (39.6%), 17:0 (7.6%), and iso-15:0 (37.8%). The 16S rRNA gene sequence similarity between the isolate CS47 and other Bacillus strains varied from 93% to 98%. In the phylogenetic analysis based on these sequences, the isolate CS47 and Bacillus amyloliquefaciens clustered within a group and separated from other species of Bacillus. Based on the physiological and molecular properties, the isolate CS47 was classified within the genus Bacillus as Bacillus amyloliquefaciens CS47. The optimal pH and temperature for mannanase activity of B. amyloliquefaciens CS47 were pH 6.0 and $50^{\circ}C$, respectively. The thermal stability of mannanase from B. amyloliquefaciens CS47 is valuable when using this enzyme in industrial application.

• Journal of Microbiology
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• v.35 no.4
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• pp.295-299
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• 1997

Pseudomonas sp. S-47 is capable of transforming 4-chlorobenzoate to 4-chlorocatechol which is subsequently oxidized bty meta-cleavage dioxygenase to prodyce 5-chloro-2-hydroxymuconic semialdehyde. Catechol 2,3-dioxygenase (C23O) produced by Pseudomonas sp. S-47 was purified and characterized in this study. The C23O enzyme was maximally produced in the late logarithmic growth phase, and the temperature and pH for maximunm enzyme activity were $30{\sim}35^{\circ}C$ and 7.0, respectively. The enzyme was purified and concentrated 5 fold from the crude cell extracts through Q Sepharose chromatography and Sephadex G-100 gel filtration after acetone precipitation. The enzyme was identified as consisting of 35 kDa subunits when analyzed by SDS-PAGE. The C23O produced by Pseudomonas sp. S-47 was similar to Xy1E of Pseudomonas putida with respect to substrate specificity for several catecholic compounds.

• 한국전자현미경학회:학술대회논문집
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• 1999.05a
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• pp.47-47
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• 1999

• Vending industry
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• v.10 no.2 s.31
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• pp.47-47
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• 2011

• Monthly Duck's Village
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• s.35
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• pp.47-47
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• 2006