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http://dx.doi.org/10.5352/JLS.2009.19.12.1724

Isolation and Characterization of Mannanase Producing Bacillus amyloliquefaciens CS47 from Horse Feces  

Cho, Soo-Jeong (Department of Microbiological Engineering, Jinju National University)
Publication Information
Journal of Life Science / v.19, no.12, 2009 , pp. 1724-1730 More about this Journal
Abstract
The mannanase-producing bacteria, designated CS47, was isolated from the fresh feces of three horses (from a farm in Jinju National University). The isolate CS47 was facultatively anaerobic and grew at temperatures ranging from $20^{\circ}C$ to $50^{\circ}C$ with an optimal temperature of $38^{\circ}C$. The DNA G+C content of the isolate CS47 was 44 mlo%. The major fatty acids were anteiso-15:0 (39.6%), 17:0 (7.6%), and iso-15:0 (37.8%). The 16S rRNA gene sequence similarity between the isolate CS47 and other Bacillus strains varied from 93% to 98%. In the phylogenetic analysis based on these sequences, the isolate CS47 and Bacillus amyloliquefaciens clustered within a group and separated from other species of Bacillus. Based on the physiological and molecular properties, the isolate CS47 was classified within the genus Bacillus as Bacillus amyloliquefaciens CS47. The optimal pH and temperature for mannanase activity of B. amyloliquefaciens CS47 were pH 6.0 and $50^{\circ}C$, respectively. The thermal stability of mannanase from B. amyloliquefaciens CS47 is valuable when using this enzyme in industrial application.
Keywords
Horse feces; mannanase activity; locust bean gum; Bacillus amyloliquefaciens CS47;
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