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Effects of Glucose on the Cleavage and Further Development of Early Bovine Embryos (Glucose가 소 초기배의 분할 및 발육에 미치는 영향)

  • 노상호;이병천;황우석
    • Journal of Embryo Transfer
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    • v.12 no.2
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    • pp.161-169
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    • 1997
  • This study was conducted to compare the insemination time of bovine oocytes and determine the effects of glucose(1.5 mM) on the development of bovine embryos at early cleavage stage. Oocytes were matured for 24 h, followed by exposure to sperm and cultured in modified Tyrode's media drops or with bovine oviduct epithelial cell monolayer prepared in TCM199(BOECM). Insemination time and culture system were varied in each experiment. In experiment 1, to investigate the developmental capacity of bovine embryos after different time of exposure to sperm, bovine ova and sperm were co-incubated for 18, 30 or 54 h, respectively. The development to blastocysts of 30 and 54 h insemination groups were significantly higher(P<0.05) than 18 h group, and in case of blastocysts of cleaved embryos, 30 h group were significantly higher(P<0.05) than other groups. In experiment 2, we investigated the effect of glucose on early bovine embryos. After 18 h insemination, in vitro fertilized oocytes were separated following 3 groups ; G+0, C+24 and C+48. Oocytes of G+0 group were cultured in glucose added Tyrode's medium after fertilization, oocytes in C+24 and C+48 groups were cultured in glucose free Tyrode's medium after fertilization. After 24 h culture, G+24 group was moved to glucose added medium. All oocytes of 3 groups were moved to BOECM after 48 h culture. The rates of cleavage and development to blastocysts in G+0 group were significantly lower than other groups. In experiment 3, we determined the effects of glucose exposure from 8 to 20 h after insemination on the cleavage and development of oocytes. The oocytes in glucose added group had high capacity of cleavage and further development. This study shows that in bovine oocytes, the optimal exposure to sperm is 30 h and glucose exposure to bovine one-cell embryos is detrimental to their first cleavage and further development in vitro but there has no evidence of detrimental effect of glucose(1.5 mM) exposure to bovine embryos over the two-cell stage in vitro.

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Nematodes Associated with Forest Trees in Korea IV. Distribution and Species of Nematodes Associated with Forest Trees (한국에 있어서 림목에 기생하는 선충조사 IV. 수목 기생선충의 종류 및 분포)

  • 최영열;추호열;김영진;문일성;백현실
    • Korean journal of applied entomology
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    • v.31 no.4
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    • pp.427-451
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    • 1992
  • The nematodes associated with forest trees were surveyed in the National Parks and Mountains in Korea. A total of 73 species on 28 genera were identified from rhizosphere of 154 tree s species. Platycarya strobilacea, Ulmus davidiana and Acer ginnala were known to new host p plants of Meloinema kerongens. Bursaphelenchus xylophilus was detected only in Pusan on Pi$\pi$us t thunbergii, B. mucronatus was detected in Cheju, Masan, and Chinhae. Insect parasitic nemat todes, Prothallonema intermedium, on P. rigida in Daegu, P. thunbergii in Cheju city, Namwonu up, Gujoa-up and Aewol-up. The number of nematode species according to forest trees was t the highest on P. thunbergii to 32 species, followed by 24 species on Abies holophylla, 20 species on Zelkova serrata, 19 species on Diospyros kaki, 18 species on Quercus acutissima, respectively. L Localities of distribution according to nematode species were as follows; Crico$\pi$emella i$\pi$formis i in 81, X. americanum in 74, Tylenchorhynchus claytoni in 51, C. macrodora in 35, H Hemicriconemoides varionodus and Ogma serratum in 31, Pararotylenchus pini in 25, H Hemicycliophora koreana in 24, Aphelenchus avenae and C. pseudohercyniensis in 24, respectively.

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'카존에어24' 차량용 살균탈취자판기

  • 한국자동판매기공업협회
    • Vending industry
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    • v.5 no.2 s.14
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    • pp.29-31
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    • 2005
  • 최근 유통분야의 웰빙 트랜드는 거부 할 수 없는 흐름이다. 정신적, 육체적으로 고급화된 소비를 지향하는 경향은 많은 산업분야에서 유행처럼 번지고 있다. 이런 마당에 자판기 역시 웰빙을 지향하는 트랜드에 관심을 안 가질 수없다. 최근 DK벤딩에서 전개 중인 '카존에어 24' 차량용 살균탈취자판기는 이러한 동향에 부합하는 제품으로 손색이 없다. 이 제품은 차량내의 나쁜 균들을 살균할 뿐만 아니라 냄새까지 없애줘 쾌적한 차량 내 환경을 제공해 준다. 출시되자마자 좋은 반응을 얻고 있어 올 하반기 시장의 히트상품으로의 부상이 예상되는 '카존에어 24'차량용 살균탈취자판기를 소개한다.

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The Changes in Korean Children's Clothing Brands - From 1990 to 2019 - (론칭 시기에 따른 국내 아동복브랜드의 변화 - 1990년부터 2019년까지 -)

  • Kim, Kyungok;Chun, Jongsuk
    • Fashion & Textile Research Journal
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    • v.24 no.1
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    • pp.108-116
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    • 2022
  • The children's clothing market continues to grow in Korea, despite the declining total fertility rate. This study aims to investigate the changes in Korean children's clothing market according to the launching time of brands. To this end, it analyzed 90 children's clothing brands in 2019. The results of this study are as follows. These children's clothing brands had been actively launched since 2000. Although the total fertility rate in Korea has dropped to approximately one percent, the number of children's clothing brands has increased significantly each year since 2005. Sixteen children's clothing brands were launched between 2005 and 2009, twenty-three were launched between 2010 and 2014, and thirty-five were launched between 2015 and 2019. Various brands are competing in Korean children's clothing market. Overseas brands have continued to expand their market share while the market share of domestic brands has decreased continuously. Clothing brands, including adult clothing brands other than children's clothing brands, are actively entering Korean children's clothing market. Many overseas, luxury, and sportswear brands have entered Korean children's clothing market. Many sportswear brands have launched children's clothing brands, with a significant increase since 2013. The results of this study show that Korean children's clothing market has changed from a local market to a global fashion market.

Antioxidant Responses in Brackish Water Flea Diaphanosoma celebensis - Exposed to Mercury (수은 노출에 대한 기수산 물벼룩 Diaphnosoma celebensis의 항산화 반응)

  • Bae, Chulhee;Lee, Young-Mi
    • Journal of Marine Life Science
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    • v.3 no.2
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    • pp.74-80
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    • 2018
  • Mercury (Hg) poses a threat to marine ecosystem due to continuous inflow from various industries and bioaccumulation to higher trophic level via food web. Mercury can adversely affect growth, development, reproduction and metabolism to aquatic organisms. In the present study, acute toxicity and oxidative stress markers (total glutathione content, and activities of GST, GR and GPx) were investigated in brackish water flea Disphanosoma celebensis exposed to HgCl2 for 24 h. As results, Hg showed negative effect in survival of D. celebensis. 24 h-LC50 value was determined as 0.589 mg/l (95% C.I. 0.521~0.655 mg/l). After exposure to Hg (0.08 and 0.4 mg/l) for 24 h, total glutathione content was significantly decreased, whereas GST, GPx and GR activities were enhanced. These findings indicate that Hg induced oxidative stress in D. celebensis, and oxidative stress markers may be involved in cellular defense against Hg - mediated toxicity. This study provides a better understanding of molecular mode of action of Hg toxicity in this specie and potent of molecular markers for heavy metal monitoring in marine ecosystem.

A study of Literature Review on the acupuncture and moxibution treatments for stomatopathy (구중질환(口中疾患)의 침구치료(鍼灸治療)에 관한 고찰(考察))

  • Youn, Hyoun-Min;Ahn, Chang-Bum;Kim, Cheol-Hong
    • Korean Journal of Acupuncture
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    • v.21 no.1
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    • pp.175-199
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    • 2004
  • Objectives : To study acupuncture and moxibution treatments for stomatopathy( aphthae, oral ulceration, mycolic stomatitis, halitosis, thirst, bitter) the ancient and the present literatures were reviewed. Methods : We've got compared and analyzed 55 kinds of literatures. Results and Conclusions : 1. The acupuncture meridians used frequently for stomatopathy were $Su-yangmy\bar{o}ng-Taejang-ky\bar{o}ng(LI),\;Chok-yangmy\bar{o}ng-Wi-Ky\bar{o}ng(S),\;Immaek-Ky\bar{o}ng(CV)$. 2. The acupoints used frequently for aphthae were $Sungjang(CV_{24}),\;Yomchon(CV_{23}),\;Hapkok(LI_4),\;Nogung(P_8),\;Chok-samni(S_{36}),\;Hyopko(S_6),\;Sugu(GV_{26})$. 3. The acupoints used frequently for oral ulceration were $Hapkok(LI_4),\;Nogung(P_8),\;Chok-samni(S_{36}),\;Kokchi(LI_{11}),\;Sotaek(SI_1),\;Pisu(B_{20}),\;Wisu(B_{21}),\;Samgan(LI_3),\;Yomchon(CV_{23}),\;Chichang(S_4)$. 4. The acupoints used frequently for mycolic stomatitis were $Hapkok(L_4),\;Chichang(S_4),\;Hyopko\;(LI_4),\;Sungjang(CV_{24}),\;Samumgyo(SP_6)$. 5. The acupoints used frequently for halitosis were $Naejong(S_{44}),\;Chok-samni(S_{36}),\;Chung-wan\;(CV_{12}),\;Sang-wan(CV_{13}),\;Hawan(CV_{10}),\;Kongson(SP_4),\;Wisu(B_{21}),\;Nogung(P_8),\;Sugu(GV_{26}),\;Sungjang(CV_{24})$. 6. The acupoints used frequently for thirst were $Sosang(L_{11}),\;Sangyang(LI_1),\;Sotaek(SI_1),\;Kwanch'ung(TE_1),\;Ch'\bar{o}kt'aek(L_5),\;T'ae-gye(K_3),\;Kokt'aek(P_3),\;Sugu(GV_{26}),\;Samgan(LI_3),\;Igan(LI_2),\;T'aech'ung(Liv_3),\;Sojangsu(B_{27})$. 7. The acupoints used frequently for bitter were $Yangn\bar{u}ngch'\bar{o}n(G_{34}),\;Hy\bar{o}njong(G_{39}),\;Kwanch'ung(TE_1),\;Tamsu(B_{19}),\;Chokkyu\bar{u}m(G_{44}),\;Y\bar{o}n-gok(K_2),\;Shinmun(H_7),\;Chok-Samni(S_{36})$.

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EFFECT OF DIFFERENT LEVELS OF ROASTED FULL-FAT SOYBEAN ON THE APPARENT DIGESTIBILITY OF DIETARY COMPONENTS BY CARP (Cyprinus carpio) GROWER

  • Kim, J.D.;Kim, K.S.;Song, J.S.;Woo, Y.B.;Jeong, K.S.;Won, T.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.8 no.6
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    • pp.635-639
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    • 1995
  • Digestibility trial was performed with carps weighing 200 g/fish. Fecal collection was made over 7 days using a settling column. The water temperature was in the range of 27 to $30{^{\circ}C}$ during whole experimental period. Fishes were fed 5 diets (Control, $F_{24}S_{13}$, $F_{16}S_{27}$, $F_8S_{40}$ and $F_0S_{56}$) containing both 32%, 24%, 16%, 8% and 0% of fish meal (F) and 0%, 13%, 27%, 40% and 56% of roasted full-fat soybean (S), respectively. Water volume in each recirculated rearing tank was maintained at 130 l with flow rate of 10-12 l/min. Apparent digestibility coefficients (ADCs) of dry matter, protein, lipid, total carbohydrates and energy in diets showed a reduction (p < 0.05) with the increase of dietary full-fat soybean level, although there was no difference in the ADCs between Control and $F_{24}S_{13}$ (p > 0.05). The ADCs of ash, Ca and P in diets significantly increased with the increase of dietary full-fat soybean level, suggesting the high availability of phosphorus in monocalcium phosphate.

Study on Basic Wind Speed Suiteable for Wind Power Development (풍력발전에 적합한 기본풍속 연구)

  • Kim, JungHwan;Jeong, HoSeong;Kim, HyeongJun;Han, JungHun;Park, SunKyu;Choi, JinWoong
    • 한국신재생에너지학회:학술대회논문집
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    • 2010.11a
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    • pp.189.1-189.1
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    • 2010
  • The wind farm where the wind velocity condition is excellent and economical can be established to produce power with the multiple wind power turbine. The wind velocity which is suitable to Wind Power Development must be evaluated for searching the economical wind farm on planning the wind farm. In this paper, based on wind speed data at 24 locations in Korea from 1971 through 2009, the basic wind velocity which can be applied to designing wind power development is estimated using the statiscal process. The wind velocity which is measured from observation stations is revised according to wind gauge's height and Circumferential environment. The wind speeds for 200 year's return period in 24 locations are determined using the Gumbel's distribution.

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Dynamic Contrast-Enhanced MR Imaging of Tietze's Syndrome: a Case Report

  • Kim, Dong Chan;Kim, Sang Yoon;Kim, Bong Man
    • Investigative Magnetic Resonance Imaging
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    • v.24 no.1
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    • pp.55-60
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    • 2020
  • Tietze's syndrome is an inflammatory condition associated with painful swelling of the costochondral, costosternal, and sternoclavicular joints. Tietze's syndrome has been mostly attributed to microtrauma until now; however, this etiology is currently disputed. The diagnosis is based on clinical findings, although a few studies suggest the advantages of imaging. We report a case of Tietze's syndrome with a review of radiological findings, especially magnetic resonance imaging (MRI) with dynamic contrast enhancement.

A cautionary note on the use of Cook's distance

  • Kim, Myung Geun
    • Communications for Statistical Applications and Methods
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    • v.24 no.3
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    • pp.317-324
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    • 2017
  • An influence measure known as Cook's distance has been used for judging the influence of each observation on the least squares estimate of the parameter vector. The distance does not reflect the distributional property of the change in the least squares estimator of the regression coefficients due to case deletions: the distribution has a covariance matrix of rank one and thus it has a support set determined by a line in the multidimensional Euclidean space. As a result, the use of Cook's distance may fail to correctly provide information about influential observations, and we study some reasons for the failure. Three illustrative examples will be provided, in which the use of Cook's distance fails to give the right information about influential observations or it provides the right information about the most influential observation. We will seek some reasons for the wrong or right provision of information.