• Title/Summary/Keyword: S-100 Standard

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Standard- and large-sized eggs of Trichuris trichiura in the feces of schoolchildren in the Yangon Region, Myanmar: Morphological and molecular analyses

  • Seungwan Ryoo;Bong-Kwang Jung;Sooji Hong;Hyejoo Shin;Hyemi Song;Hyun-Seung Kim;Jin-Youp Ryu;Woon-Mok Sohn;Sung-Jong Hong;Thi Thi Htoon;Htay Htay Tin;Jong-Yil Chai
    • Parasites, Hosts and Diseases
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    • v.61 no.3
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    • pp.317-324
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    • 2023
  • Standard- and large-sized eggs of Trichuris trichiura were found in the feces of schoolchildren in Yangon, Myanmar during epidemiological surveys and mass deworming with albendazole in 2017-2019. The standard-sized eggs were identified as those of T. trichiura, but it was necessary to exclude the possibility of the large-sized eggs belonging to Trichuris vulpis, a dog whipworm. We conducted morphological and molecular studies to determine the species of the 2 types of Trichuris eggs. Individual eggs of both sizes were isolated from Kato-Katz fecal smears (n=20) and mechanically destroyed using a 23G injection needle. Nuclear DNA was extracted, and the 18S rRNA region was sequenced in 15 standard-sized eggs and 15 large-sized eggs. The average size of standard-sized eggs (T. trichiura) was 55.2×26.1 ㎛ (range: 51.7-57.6×21.3-28.0 ㎛; n=97), whereas the size of large-sized eggs was 69.3×32.0 ㎛ (range: 65.1-76.4×30.1-34.5 ㎛; n=20), slightly smaller than the known size of T. vulpis. Regarding standard-sized eggs, the 18S rRNA nucleotide sequences exhibited 100% homology with T. trichiura deposited in GenBank and 88.6-90.5% homology with T. vulpis. Regarding large-sized eggs, the nucleotide sequences showed 99.8-100% homology with T. trichiura in GenBank and 89.6-90.7% homology with T. vulpis. Both standard- and large-sized eggs of Trichuris spp. found in Myanmar schoolchildren during 2017-2019 were morphologically and molecularly confirmed to belong to T. trichiura. The conversion of eggs from smaller to large sizes might be due to anthelmintic treatments with albendazole.

Quantifying Uncertainty of Calcium Determination in Infant Formula by AAS and ICP-AES (AAS 및 ICP-AES에 의한 조제분유 중 칼슘 함량 분석의 측정불확도 산정)

  • Jun, Jang-Young;Kwak, Byung-Man;Ahn, Jang-Hyuk;Kong, Un-Young
    • Korean Journal of Food Science and Technology
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    • v.36 no.5
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    • pp.701-710
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    • 2004
  • Uncertainty was quantified to evaluate calcium determination result in infant formula with AAS (Atomic Absorption Spectrometry) and ICP-AES (Inductively Coupled Plasma-Atomic Emission Spectrometry). Uncertainty sources in measurand, such as sample weight, final volume of sample, sample dilution and the instrumental result were identified and used as parameters for combined standard uncertainty based on the GUM (Guide to the expression of uncertainty in measurement) and Draft EURACHEM/CITAC Guide. Uncertainty components of each sources in measurand were identified as resolution, reproducibility and stability of chemical balance, standard material purity, standard material molecular weight, standard solution concentration, standard solution dilution factor, sample dilution factor, calibration curve, recovery, instrumental precision, reproducibility, and stability, Each uncertainty components were evaluated by uncertainty types and included to calculate combined uncertainty. The kinds of uncertainty sources and components in the analytical method by AAS and ICP-AES were same except sample dilution factor for AAS. The analytical results and combined standard uncertainties of calcium content were estimated within the certification range $(367{\pm}20\;mg/100g)$ of CRM (Certified Reference Material) and were not significantly different between method by AAS followed by ashing and method by ICP-AES followed by acid digestion as $359.52{\pm}23.61\;mg/100g\;and\;354.75{\pm}16.16\;mg/100g$, respectively. Identifying uncertainty sources related with precision, repeatability, stability, and maintaining proper instrumental conditions as well as personal proficiency was needed to reduce analytical error.

Uncertainty Analysis of BAG by GNSS Correction (해저지형 표면자료의 GNSS 보정방법에 따른 불확실도 연구)

  • OH, Che-Young;KIM, HO-Yong;LEE, Yun-Sik;CHOI, Chul-Uong
    • Journal of the Korean Association of Geographic Information Studies
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    • v.22 no.3
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    • pp.1-9
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    • 2019
  • In the recent marine sector, the development and standardization regarding S-100, which is the universal hydrographical data model standard for development of marine space information, was progressed, and for the effectiveness of marine chart production work and the multi-purpose use of water level data in S-100, S-102(Bathymetric Surface grid) standard development and various studies of BAG formats combined with water level and uncertainty, property information is being progressed. Since the water level information that is important in the operation of the ship is provided based on S-102, the calibration method of the location information when producing S-102 is an important factor in deciding the water level. In this study, the hydrographical surveying was conducted by piloting the standardized method for the production of S-102 in Korea, and have compared the accuracy of water level information according to the GNSS post treatment calibration method. As a result of comparing the water level in 2 places in the rocky terrain of the study area, the northern water level of Namu-do was shown as DL 0.79~0.83m, the eastern water level of Daeho-do was DL 12.63~12.91m, and the horizontal position errors of the intermittent sunshine water level were confirmed to be within 1m. As a result, the intermittent sunshine water level according to the location calibration method when producing the BAG was confirmed that it was in the available range for a ship's safe voyage. However, the accuracy verification for the location of the ship when conducting hydrographical surveying was judged that there is a need for a various additional study about regional characteristics and environment factor.

Antimicrobial Activity of the Essential Oils of Artemsia Princeps var. orientalis (쑥으로부터 추출한 정유의 항균효과)

  • 안병용
    • Journal of Food Hygiene and Safety
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    • v.7 no.4
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    • pp.157-160
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    • 1992
  • The essential oils of Aritemisia princeps var. orientalis (wonnwood) were tested against the standard cultures &cherichia coli, Bacillus subtilis, Pleurotus oststreatus. Fusarium soiani, Aspergillus niduians. Escherichia coli was not susceptible to the wonnwood essential oil but the growth of Bacillus subtilis, Aspergillus niduians, Fusarium solani and Pleurotus ostreatus was severely inhibited by essential oil. The growth of Bacillus subtilis in 1O~100 ppm was a tenth of the control. The wonnwood essential oil also exhibited strong inhibited of the growth of tested fungi. The growth of Pleurotus ostreatus was fully stopped at 1,000 ppm concentration.

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Comparison of LAL Test with Pyrogen Test (발열성 물질시험과 세균 내독소 시험의 비교 연구)

  • 이유경;강윤숙;백선영;김용관;신광훈;민홍기
    • YAKHAK HOEJI
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    • v.43 no.5
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    • pp.606-613
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    • 1999
  • To survey the possibility of replacing the pyrogen test with Limulus Amebocyte Lysate(LAL) test and to find out a standard methods suitable to our blood products made in Korea, 100 samples of 20% human serum albumin were tested by commercial LAL test kits and results of those were compared with rabbit pyrogen test. The LAL test is used both dinetic-chromogenically and kinetic-turbidimetrically. Both methods equally showed broad detection range (5.0~0.005 EU/ml), excellent sensitivity ($\geq$ 0.005 EU/ml) and predominant recovery rate within valid dilution range, but kinetic-turbidimetric method seemed to be more reproducible than kinetic-chromogenic method(kinetic-chromogenic method : S.D. = 15.88, kinetic-turbidimetric method : S.D. = 8.12). After heating the sample at 75$^{\circ}C$ for 15 min, the results showed a little elevated recovery rate with both methods. After performing the test on 100 albumin samples with both kits, the results were analysed using the USP standard (1.33 EU/ml). 7% of samples in kinetic-chromogenic methods and 1% of samples in kinetic-turbidimetric method exceeded the limit of endotoxin levels regulated for blood products in USA. Because this phenomenon was not observed in both methods at the same time and both methods have high sensitivity ($\geq$0.005 EU/ml), these results seemed to depend on nonspecific reaction. Considering its sensitivity and reproducibility, we could assure that LAL test is proper to detecting pyrogenic with good sensitivity.

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Simulation-based analysis of total ionizing dose effects on low noise amplifier for wireless communications

  • Gandha Satria Adi;Dong-Seok Kim;Inyong Kwon
    • Nuclear Engineering and Technology
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    • v.56 no.2
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    • pp.568-574
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    • 2024
  • The development of radiation-tolerant radio-frequency (RF) systems can be a solution for applications in extreme radiation environments, such as nuclear power plant monitoring and space exploration. Among the crucial components within an RF system, the low noise amplifier (LNA) stands out due to its vulnerability to TID effects, mainly relying on transistors as its main devices. In this study, the TID effects in the LNA using standard 0.18 ㎛ complementary metal oxide semiconductors (CMOS) technology are estimated and analyzed. The results show that the LNA can withstand absorbed radiation up to 100 kGy. The S21, S11, noise figure (NF), stability (K), and linearity of the third input intercept point (IIP3) slightly shifted from the initial values of 0.8312 dB, 0.793 dB, 0.00381 dB, 1.34406, and 2.36066 dBm, respectively which are still comparable to the typical performances. Moreover, the standard 0.18 ㎛ technology has demonstrated its radiation tolerance, as it exhibits negligible performance degradation in the conventional LNA even when exposed to radiation levels up to 100 kGy. In this context, simulation approach offers a means to predict the TID effects and estimate the radiation exposure limit for electronic devices, particularly when transistors are used as the primary RF components.

A Study on Parallax Registration for User Location on the Transparent Display using the Kinect Sensor (키넥트 센서를 활용한 투명 디스플레이에서의 사용자 위치에 대한 시계 정합 연구)

  • Nam, Byeong-Wook;Lee, Kyung-Ho;Lee, Jung-Min;Wu, Yuepeng
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.28 no.6
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    • pp.599-606
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    • 2015
  • International Hydrographic Organization(IHO) adopted standard S-100 as the international standard Geographic Information System(GIS) that can be generally used in the maritime sector. Accordingly, the next-generation system to support navigation information based on GIS standard technology has being developed. AR based navigation information system that supported navigation by overlapping navigation information on the CCTV image has currently being developed. In this study, we considered the application of a transparent display as a method to support efficiently this system. When a transparent display applied, the image distortion caused by using a wide-angle lens for parallax secure, and the disc s, and demonstrated the applicability of the technology by developing a prototype.

Effect of a polyherbal formulation on anxiety and behaviour mediated via monoamine neurotransmitters

  • Balaraman, R;Mohan, M;Aurangabadkar, VM;Jadhav, GB;Austin, Anoop;Thirugnanasampathan, Thirugnanasampathan
    • Advances in Traditional Medicine
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    • v.7 no.4
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    • pp.409-417
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    • 2007
  • We investigated the effect of Arogh, a polyherbal formulation (PHF) on animal models of anxiety based on exploratory behavior. The anxiolytic activity of polyherbal formulation (30, 100, 300 and 500 mg/kg) was studied using various behavioural paradigms such as elevated plus maze (EPM), light/dark apparatus (LDA), open field apparatus (OFA), hole board apparatus (HBA). Diazepam (1 mg/kg) was used as a standard anxiolytic drug. The effect of PHF (100 and 300 mg/kg) on serotonin, dopamine and noradrenaline mediated behaviour was studied by lithium induced head twitches in rats, haloperidol induced catalepsy in mice and clonidine induced hypothermia in rats respectively. In EPM, PHF (100, 300 and 500 mg/kg) significantly (P < 0.05) increased the time spent in open arms and the number of entries in open arms. In LDA, PHF (100, 300 and 500 mg/kg) significantly (P < 0.05) increased the time spent in lit zone. In OFA, PHF (100, 300 and 500 mg/kg) significantly (P < 0.05) increased the number of assisted rearing and the number of squares traversed. In HBA, PHF (100, 300 and 500 mg/kg) significantly (P < 0.05) increased the number of head poking. In lithium induced head twitches, PHF (100 and 300 mg/kg) significantly (P < 0.05) decreased the number of head twitches. In haloperidol induced catalepsy, PHF (300 mg/kg) decreased the duration of catalepsy significantly (P < 0.05) at 60 min. In clonidine-induced hypothermia, PHF (300 mg/kg) did not modify the effect. Drugs must be carefully assessed on EPM test and therefore in the present study EPM is supported by other tests. Present study indicates that Arogh, a polyherbal formulation possess anxiolytic activity. It diminished serotonergic transmission and decreased the duration of catalepsy indicating potentiation of dopaminergic transmission. Thus, Arogh a polyherbal formulation contains bioactive principles which possess anxiolytic activity and modified 5-HT and DA mediated behaviour.

Calibration and Uncertainty Measurement of Differential Mobility Analyzer Using 100 nm NIST SRM 1963 (100 nm NIST 표준입자를 이용한 미분형 전기 이동도 분석기의 교정 및 불확실도 측정)

  • Lee, Snag-Jin;Ahn, Jin-Hong;Ahn, Kang-Ho
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.27 no.12
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    • pp.1766-1771
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    • 2003
  • House made differential mobility analyzer(DMA) is calibrated with NIST SRM 1691(300 nm PSL). Then the particle size and uncertainty for differential mobility analyzer(DMA) using the NIST SRM 1963(100 nm PSL). In result, calibration of prototype DMA is measured using 300 nm NIST SRM 1691, then sheath air flow was corrected 126.67 ㎤/s. Corrected sheath air flow is used in uncertainty measurement of prototype DMA. Uncertainty analysis is performed using NIST SRM 1963(100 nm PSL). The experimental result shows that NIST SRM 1963 is measured as 102.17 nm with a type A uncertainty of 0.33 nm.