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Effects of Fowl Dropping, Saw Dust and Rice Hull on Soil Microflora in vitro (실내배양에서 생계분, 톱밥 및 왕겨 첨가가 토양미생물상에 미치는 효과)

  • Yang, Chang-Sool;Kong, Hye-Suk
    • Korean Journal of Soil Science and Fertilizer
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    • v.29 no.1
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    • pp.53-59
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    • 1996
  • This study was conducted to evaluate the effects of fowl dzopping. saw dust and rice hall on the soil microflora in vitro. The experiment was designed in seven treatments with the various organic materials and they were only soil (control). soil + fowl dropping (S+F), soil+fowl dropping+rice hull (S+F+R) soil+fowl dropping*saw dust (S+F+S). soil+chemical fertilizer (S+C.F), fowl dropping+rice hull (F+R) and fowl dropping+saw dust (F+S). All the samples of treatment were incubated in $28{\pm}2^{\circ}C$ condition and tested the activity of soil microflora for 84 days The activity of fungi, total bacteria, gram-negative bacteria and actinomycetes showed the highest values at, twenty-first day and the spore-forming bacteria was at forty-second day after incubation. The number of fungi and gram-negative bacteria showed the highest values in the treatment of F+S, the spore-forming bacteria and the actinomycetes were in the S+F+S. and the number of total bacteria was in the F+C.F., but in the treatment of F+R. all the microorganism except fungi showed the lowest values in their numbers. The composition ratio of dead bacteria was higher in the treatments of S+F+R and F+R than in those of others as 70% and 40% respectively. Actinomycetes isolated from the treatments of S+F and S+F+S were identified as Streptomyces sp.. Nocardia sp., Micromonospora sp. Actinomadura sp. and Saccharomonospora sp.

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Prion Protein Genotypes in Pakistani Goats

  • Babar, M.E.;Nawaz, M.;Nasim, A.;Abdullah, M.;Imran, M.;Jabeen, R.;Chatha, S.A.;Haq, A.U.;Nawaz, A.;Mustafa, H.;Nadeem, A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.7
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    • pp.936-940
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    • 2008
  • The PCR-amplified prion protein (PrP) gene was sequenced to determine the frequency of scrapie-associated as well as novel PrP genotypes in 72 healthy goats representing five breeds. A total of six genotypes were detected, resulting from the three reported 143 (H/R), 154 (R/H) and 240 (S/P) and the two novel 39 (S/R) and 185 (I/F) amino acid polymorphisms. Of the four silent mutations 42 (a$\rightarrow$g), 138 (c$\rightarrow$t), 231 (c$\rightarrow$a) and 237 (g$\rightarrow$c) detected in this study, 237 (g$\rightarrow$c) is novel. A genotype (SIP/RFP) harboring three amino acid polymorphisms 39 (S/R), 185 (I/F) and 240 (S/P) was found in few goats. Although both scrapie-associated genotypes with 143 (H/R) and 154 (R/H) polymorphisms and others with 39 (S/R), 185 (I/F) and 240 (S/P) polymorphisms were present in the studied Pakistani goats, their frequency was lower than that of the wild-type genotype SHRIS/SHRIS (34.7%). These results emphasize the need for further sequencing of the PrP gene in a large number of goats representing the five studied breeds, so that overall PrP variability can be assessed in these breeds in research addressing future concerns about scrapie.

Roles of microRNA-206 in Osteosarcoma Pathogenesis and Progression

  • Bao, Yun-Ping;Yi, Yang;Peng, Li-Lin;Fang, Jing;Liu, Ke-Bin;Li, Wu-Zhou;Luo, Hua-Song
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.6
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    • pp.3751-3755
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    • 2013
  • Backgroud and Aims: MicroRNA-206 has proven to be down-regulated in many human malignancies in correlation with tumour progression. Our study aimed to characterize miR-206 contributions to initiation and malignant progression of human osteosarcoma. Methods: MiR-206 expression was detected in human osteosarcoma cell 1ine MG63, human normal osteoblastic cell line hFOB 1.19, and paired osteosarcoma and normal adjacent tissues from 65 patients using quantitative RT-PCR. Relationships of miR-206 levels to clinicopathological characteristics were also investigated. Moreover, miR-206 mimics and negative control siRNA were transfected into MG63 cells to observe effects on cell viability, apoptosis, invasion and migration. Results: We found that miR-206 was down-regulated in the osteosarcoma cell line MG63 and primary tumor samples, and decreased miR-206 expression was significantly associated with advanced clinical stage, T classification, metastasis and poor histological differentiation. Additionally, transfection of miR-206 mimics could reduce MG-63 cell viability, promote cell apoptosis, and inhibit cell invasion and migration. Conclusions: These findings indicate that miR-206 may have a key role in osteosarcoma pathogenesis and development. It could serve as a useful biomarker for prediction of osteosarcoma progression, and provide a potential target for gene therapy.

Hydration of Portland Cement Containing Blending Components by Impedance Spectroscopy (임피던스 측정에 의한 혼합재를 포함한 포틀랜드 시멘트의 수화)

  • 송종택;김훈상;배승훈
    • Journal of the Korean Ceramic Society
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    • v.39 no.6
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    • pp.540-549
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    • 2002
  • In this work, the hydration behaviour of portland cement pastes containing fly ash, blast-furnace slag and silica fume is investigated by Impedance Spectroscopy(IS). As fly ash or blast-furnace slag was added to portland cement, the values of R$_{t(s+1)}$ and R$_{t(s+1)}$ were decreased in the early hydration period. It showed that hydration of cement containing blending components was slower than it of the reference cement paste with the same W/C ratio. However, the cement paste containing silica fume had a large value of R$_{t(s+1)}$ and R$_{t(int)}$due to very rapid pozzolanic reactivity of silica fume in the hydration time. In OPC-fly ash system, a characteristic plateau region appeared between straight-line and semicircle. The plateau region continued to grow in range with the content of fly ash and the hydration time.

Mineralogy of Illite/smectite Mixed-Layer Clays from the Beaufort-Mackenzie Basin, Arctic Canada (카나다 보포트-맥켄지 분지의 일라이트/스멕타이트 혼합층 점토광물 연구)

  • Ko, Jaehong;Hesse, R.
    • Economic and Environmental Geology
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    • v.28 no.4
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    • pp.327-335
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    • 1995
  • Illite/smectite (I/S) in the Beaufort-Mackenzie Basin, Arctic Canada has been scrutinized on the basis of mineralogical analysis of 215 core and drill-cutting samples from 22 exploratory wells onshore and offshore. I/S in the Beaufort-Mackenzie Basin includes the following four types: random, a mixture of random and ordered, R1-ordered, and R>1-ordered I/S. A mixture of random and ordered I/S occurs in the transitional interval between random and R>1-ordered I/S, and may represent a metastable state in the ordering reaction. A widespread occurrence of the mixture in natural environments suggests that the ordering reaction may be a slow process that results in co-existence of reactants and products. K-saturation experiments show that layer charges of expandable layers in I/S are variable. High-charge expandable layers transform into illite-like layers upon simple K-saturation. K-saturation alters the composition and/or the degree of ordering in I/S, suggesting that illitization in nature can be transformational.

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Evaluation of SPECT Analysis in Patients with Transient Global Amnesia

  • Choe, Bo-Young;Kim, Euy-Neyng;Chung, Yong-An;Sohn, Hyung-Sun;Kim, Sung-Hoon;Chung, Soo-Kyo;Lee, Hyoung-Koo;Suh, Tae-Suk
    • Journal of the Korean Magnetic Resonance Society
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    • v.6 no.1
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    • pp.45-53
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    • 2002
  • Objectives: This study investigated alterations in regional cerebral blood flow (rCBF) in patients with transient global amnesia (TGA) using statistical parametric mapping 99 (SPM99). Methods: Noninvasive rCBF measurements using 99mTc-ethyl cysteinate dimer (ECD) SPECT were performed on 8 patients with TGA who have ongoing symptoms and 17 age matched controls. The relative rCBF maps in patients with TGA and controls were compared. Results: In patients with TGA, significant decreased rCBF was found along the L superior temporal extending to L parietal region of the brain and L thalamus. There were areas of increased rCBF in the R temporal, R frontal region and R thalamus. Conclusion: We could demonstrate decreased perfusion in left cerebral hemisphere and increased perfusion in right cerebral hemisphere in patients with TGA using SPM99. The imbalanced change of rCBF between bilateral cerebral hemisphere in patients with TGA might suggest that imbalanced neuronal activity between the bilateral hemispheres may have strong relationship to the pathogenesis of the TGA. For quantitative SPECT analysis in TGA patients, we recommend SPM99 rather than the ROI method because of its definitive advantages.

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ON ℤpp[u]/k>-CYCLIC CODES AND THEIR WEIGHT ENUMERATORS

  • Bhaintwal, Maheshanand;Biswas, Soumak
    • Journal of the Korean Mathematical Society
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    • v.58 no.3
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    • pp.571-595
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    • 2021
  • In this paper we study the algebraic structure of ℤpp[u]/k>-cyclic codes, where uk = 0 and p is a prime. A ℤpp[u]/k>-linear code of length (r + s) is an Rk-submodule of ℤrp × Rsk with respect to a suitable scalar multiplication, where Rk = ℤp[u]/k>. Such a code can also be viewed as an Rk-submodule of ℤp[x]/r - 1> × Rk[x]/s - 1>. A new Gray map has been defined on ℤp[u]/k>. We have considered two cases for studying the algebraic structure of ℤpp[u]/k>-cyclic codes, and determined the generator polynomials and minimal spanning sets of these codes in both the cases. In the first case, we have considered (r, p) = 1 and (s, p) ≠ 1, and in the second case we consider (r, p) = 1 and (s, p) = 1. We have established the MacWilliams identity for complete weight enumerators of ℤpp[u]/k>-linear codes. Examples have been given to construct ℤpp[u]/k>-cyclic codes, through which we get codes over ℤp using the Gray map. Some optimal p-ary codes have been obtained in this way. An example has also been given to illustrate the use of MacWilliams identity.

Significance of Follow-Up Ultrasonography 24 Hours Post-Reduction in Detecting Intussusception Recurrence

  • Kim, Sujin;Lim, HyeJi;Park, Sowon;Koh, Hong
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.25 no.1
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    • pp.21-29
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    • 2022
  • Purpose: The objective of this study was to identify the significance of 24-hour post-reduction ultrasonography (US) in pediatric patients with intussusception. Methods: A total of 229 patients with intussusception who were treated with saline reduction at Severance Children's Hospital between January 2014 and September 2020 were retrospectively reviewed. The 229 patients with successful saline reduction were divided into two groups: a recurrence at 24 hours group (R, n=41) and a non-recurrence group (NR, n=188). The full patient sample was divided into two groups: follow-up US (FU) or no follow-up US (NFU); the recurrence group was divided into follow-up (R-FU) and non-follow-up (R-NFU) subgroups, and stratified analyses were performed. Results: There were no significant differences in age, sex, laboratory findings, symptoms, and sonographic findings between the NR and R groups. In the R group, 24 patients underwent follow-up US, and 17 patients did not. Specific sonographic findings were statistically significant in the R-FU group compared to the R-NFU group (p=0.002). The R-FU group had fewer admissions (p=0.012) and longer mean hospitalization times (p<0.001) than the R-NFU group. The NFU group had a 12.2% recurrence rate, while the R-FU group recurrence rate was 25.8% (p=0.0099), suggesting that the omission of some recurrent events and follow-up US was a significant variable in the recurrence of intussusception. The median time to recurrence was 21 hours which supports the 24-hour follow-up protocol. Conclusion: Twenty-four-hour follow-up US was shown to be valuable for detecting early recurrence of intussusception.

Variations in Seed Storage Protein among Different Colored Soybean Varieties

  • Kim, Sun-Lim;Yun, Hong-Tae;Moon, Jung-Kyung;Park, Keum-Yong;Lee, Yeong-Ho;Ryu, Yong-Hwan
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.2
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    • pp.141-147
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    • 2004
  • This study was carried out to know the variation of soybean seed proteins, 11S and 7S globulins, and their amino acid compositions among different colored soybean varieties, 'Danbaegkong' (yellow), 'Pureunkong' (green) 'Jinyulkong' (brown), and 'Geoumjeongkong l' (black). Soybean seed proteins showed a wide range in molecular size, but the electrophoresis patterns of total seed protein subunits showed a similarity among different colored soybean varieties. Amino acid compositions of total seed proteins were similar for all soybean varieties tested. However, soybean varieties showed low composition rates in sulfur containing amino acids. The composition rates of cysteine and methionine in the 11S globulins were higher than those of total seed proteins and 7S globulins. Glutamic acid and glycine were higher in the 11S and 7S globulins than those of total seed proteins. However, the levels of methionine and phenylalanine are high in the 11S globulins, but those of valine and lysin are slightly lower than the 7S globulins. By using HPLC, we tried to analyse the soybean seed proteins. The 11S globulin was composed of 10 major peaks whereas the 7S globulin was composed of 4 major peaks. The composition rates of 11S related proteins have a tendency to increasing during the maturing whereas those of 7S related proteins have a tendency to decreasing. Composition rates of each peaks among different colored soybean varieties suggested that soybean seed proteins are varied, although they showed similarity in the electrophoresis patterns, and understanding of this characteristics is important for the utilization of soybeans.

Effect of $Bio-Silverlite^{(R)}$ on Performance, Weight of Organ, Intestinal Villus and Intestinal Microbial in Broiler Chicks (브로일러 사료에 $Bio-Silverlite^{(R)}$의 첨가가 사양성적, 장기중량, 소장 Villus 및 장내 미생물 성상에 미치는 영향)

  • Kim C.H.;Lim K.C.;Hwang J.H.;Ra C.S.;Pak J.I.
    • Korean Journal of Poultry Science
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    • v.33 no.1
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    • pp.33-39
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    • 2006
  • This study was conducted to investigate the feeding effect of $bio-silverlite^{(R)}$ on growth performance, organ phenomenon and cecum microflora in broiler chicks. The $bio-silverlite^{(R)}$ was made by an ion exchange between illite and $silver(Ag^+)$. There were four treatment groups: negative control group(non-treatment), antibiotic supplement group (positive control), 0.5% $bio-silverlite^{(R)}$ supplment group and 1.5% $bio-silverlite^{(R)}$ supplement group. Total 200 birds was assigned for this five replication tests, allocating 10 birds into each treatment. Experimental diets were formulated on isocalories and isonitrogen for the whole experimental period. Body weight gain was higher in antibiotic supplementation (+C) and $bio-silverlite^{(R)}$ supplement groups(S 0.5% and 51.5%) than the negative control group(-C), and feed efficiency was significantly enhanced with increase of the level of $bio-silverlite^{(R)}$ supplement. The length of small intestine was longer in +C than in -C and $bio-silverlite^{(R)}$ supplement groups (P<0.05), and the weight of small intestine was proportional to the level of $bio-silverlite^{(R)}$ supplement. Crop weight was lower in $bio-silverlite^{(R)}$ supplement group than in -C and +C groups (P<0.05), and the cecum weight was heavier in $bio-silverlite^{(R)}$ supplementation group. Intestinal villi height was longer in 51.5% group at 3 weeks and 6 weeks of age than in -C and +C groups. With the respect of the formation of intestinal microflora, TBC and CBC was not affected by age and feed additive. However, the number of LAB was slightly higher in $bio-silverlite^{(R)}$ supplement group than in -C and +C groups.