• Title/Summary/Keyword: S/R

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The usefulness of soluble transferrin receptor in the diagnosis and treatment of iron deficiency anemia in children

  • Yoon, Se Hoon;Kim, Dong Sup;Yu, Seung Taek;Shin, Sae Ron;Choi, Du Young
    • Clinical and Experimental Pediatrics
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    • v.58 no.1
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    • pp.15-19
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    • 2015
  • Purpose: Soluble transferrin receptor (sTfR) is a truncated extracellular form of the membrane transferrin receptor produced by proteolysis. Concentrations of serum sTfR are related to iron status and erythropoiesis in the body. We investigated whether serum sTfR levels can aid in diagnosis and treatment of iron deficiency anemia (IDA) in children. Methods: Ninety-eight patients with IDA were enrolled and were classified according to age at diagnosis. Group 1 comprised 78 children, aged 6-59 months, and group 2 comprised 20 adolescents, aged 12-16 years. Results: In group 1, patients' serum sTfR levels correlated negatively with mean corpuscular volume; hemoglobin (Hb), ferritin, and serum iron levels; and transferrin saturation and positively with total iron binding capacity (TIBC) and red cell distribution width. In group 2, patients' serum sTfR levels did not correlate with ferritin levels and TIBC, but had a significant relationship with other iron indices. Hb and serum sTfR levels had a significant inverse relationship in both groups; however, in group 1, there was no correlation between Hb and serum ferritin levels. In 30 patients of group 1, serum sTfR levels were significantly decreased with an increase in Hb levels after iron supplementation for 1 month. Conclusion: Serum sTfR levels significantly correlated with other diagnostic iron parameters of IDA and inversely correlated with an increase in Hb levels following iron supplementation. Therefore, serum sTfR levels can be a useful marker for the diagnosis and treatment of IDA in children.

Karyotype Analysis and Physical Mapping of rDNAs in Bupleurum longeradiatum (개시호 (Bupleurum longeradiatum)의 핵형분석과 rDNAs의 Physical Mapping)

  • Koo, Dal-Hoe;Seong, Nak-Sul;Seong, Jong-Suk;Bang, Kyong-Hwan;Bang, Jae-Wook
    • Korean Journal of Medicinal Crop Science
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    • v.11 no.5
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    • pp.402-407
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    • 2003
  • Karyotype analysis and chromosomal localization of 5S and 45S rDNAs using multi-color fluorescence in situ hybridization (McFISH) technique were carried out in Bupleurum longeradiatum. Somatic metaphase chromosome number was 2n=12. Karyotype was composed of three pairs of metacentrics (No.3, 4 and 6) and three pairs of submetacentrics (No. 1, 2 and 5). The length of somatic prometaphase chromosomes ranges from 2.55 to $5.05{\mu}m$ with total length of $18.15\;{\mu}m$. In FISH experiment, one pair of 5S rDNA signals was detected on the pericentromeric region of chromosome 4 and one pair of 45S rDNA signals was detected on the telomeric region of chromosome 2.

A Study on Forest Changes for A/R CDM in North Korea (A/R CDM을 위한 북한지역의 산림변화 연구)

  • Lee, Dong-Kun;Oh, Young-Chool;Kim, Jae-Uk
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.10 no.2
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    • pp.97-104
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    • 2007
  • A/R CDM(Afforestation/Reforestation Clean Development Mechanism) in Kyoto Mechanism means, either afforestation in the area used for other purposes more than 50 years or reforestation in the area used for other purposes on December 31st in 1989. South Korea has few sites due to the successful forestation in the past, but North Korea has not reforested the deforested lands since the mid-1970's. So these areas need to apply A/R CDM Project for restoration. The purposes of this study are to make a time series analysis in deforested areas and to estimate a feasibility of A/R CDM. To find the site satisfying A/R CDM business definition, land cover classification was applied using satellite images of the mid-1970's with good forestation, late 1980's including A/R CDM base year, and recent 2000's, and the chronological change was analyzed to categorize the possible sites. The North Korean topographical map of 1977 was used to verify land cover classification degree of 1970's, the land cover classification results made by the Ministry of Environment in 2000 were compared to verify the accuracy of 1980's results, and the land cover classification results in 2000's were verified by 2 site visits. The results of this study can be summarized as follows. The eligible A/R CDM sites are 605,156ha on the basis of the forestation change analysis in North Korea. Since the mid-1970's, 30.8% of the decreased forestation area of 1,966,306ha was classified into A/R CDM eligible sites. While other countries have the limited eligible sites, which has not been used for forestation since 1989 or which is being scattered, North Korea has large scale sites. Deforested sites are mainly around road and residential area, consequently give better accessibility for forestation than other countries. In conclusion, it is found that North Korea can provide efficient site for applying A/R COM Project to forestation restoring deforested land because of easy accessibility and existence of many possible sites due to artificial deforestation. Also, it is meaningful that the study suggests the application possibility of A/R COM Project to restore deforested land in North Korea and the related basic information through the chronological classification of the mid-1970's with good forestation, the late-1980's including A/R COM base year, and recent 2000's. It is expected that the study contributes to revitalization of A/R CDM Project and related research on North Korea forestation.

Isolation and Characterization of Bacteriolytic Wild Myxobacteria (용균성 야생 점액세균의 분리)

  • 박수연;이봉수;김지훈;이차율;장은혜;조경연
    • Microbiology and Biotechnology Letters
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    • v.32 no.3
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    • pp.218-223
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    • 2004
  • Myxobacteria are Gram-negative soil bacteria known to be a rich source of potentially useful secondary metabolites. We have isolated 204 strains of bacteriolytic myxobacteria from soil samples collected in Korea and determined their 16S rRNA sequences. Sequence analysis of the partially determined 16S rRNA sequences has suggested that 132 isolates (65% of total isolates) belong to the genus Myxococcus and 59 isolates (29% of total isolates) belong to the genus Corallococcus. Meanwhile, 4 isolates appear to be Archangium spp. and the other 4 isolates appear to be Stigmatella spp. Genera of the remained 5 isolates have not been identified because their 16S rRNA sequences are distantly related to those of known myxobacteria.

Development of a Novel Long-Range 16S rRNA Universal Primer Set for Metagenomic Analysis of Gastrointestinal Microbiota in Newborn Infants

  • Ku, Hye-Jin;Lee, Ju-Hoon
    • Journal of Microbiology and Biotechnology
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    • v.24 no.6
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    • pp.812-822
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    • 2014
  • Metagenomic analysis of the human intestinal microbiota has extended our understanding of the role of these bacteria in improving human intestinal health; however, a number of reports have shown that current total fecal DNA extraction methods and 16S rRNA universal primer sets could affect the species coverage and resolution of these analyses. Here, we improved the extraction method for total DNA from human fecal samples by optimization of the lysis buffer, boiling time (10 min), and bead-beating time (0 min). In addition, we developed a new long-range 16S rRNA universal PCR primer set targeting the V6 to V9 regions with a 580 bp DNA product length. This new 16S rRNA primer set was evaluated by comparison with two previously developed 16S rRNA universal primer sets and showed high species coverage and resolution. The optimized total fecal DNA extraction method and newly designed long-range 16S rRNA universal primer set will be useful for the highly accurate metagenomic analysis of adult and infant intestinal microbiota with minimization of any bias.

Enantio-Selective Inhibition of (1R,9S)- and (1S,9R)-$\beta$-Hydrastines on Dopamine Biosynthesis in PC12 Cells

  • Yin, Shou-Yu;Kim, Yu-Mi;Lee, Jae-Joon;Jin, Chun-Mei;Yang, You-Jong;Kang, Min-Hee;Lee, Myung-Koo
    • Proceedings of the PSK Conference
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    • 2003.04a
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    • pp.138.2-139
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    • 2003
  • The inhibitory effects of (1R, 9S)- and (1S, 9R)-enantiomers of $\beta$-hydrastine (BHS) on dopamine biosynthesis in PC12 cells were investigated. (1R, 9S)-BHS decreased the intracellular dopamine content with the $IC_{50}$ value of 14.3 $\mu\textrm{M}$ at 24 h, but (1S, 9R)-BHS did not. In these conditions, (1R, 9S)-BHS inhibited TH activity mainly in a concentration-dependent manner(33% inhibition at 20 $\mu\textrm{M}$) and decreased TH mRNA level. (omitted)

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Relationship of motor ability evaluation by using MAS and each items of MAS in stroke patient (Stroke 환자에 있어서 MAS(Motor Assessment Scale)를 이용한 운동 능력평가와 각각의 평가 항목과의 상관관계)

  • Kim, Kwang-Soo
    • Journal of Korean Physical Therapy Science
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    • v.4 no.4
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    • pp.519-528
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    • 1997
  • The purpose of this research were to evaluate the overall capacity of activity in hemiplegic patients caused by stroke, to learn the relationship of the overall capacity of activity with 8 out of 9 subtest of the Motor Assessment Scale (MAS) excluding general tonus subtest, and to use in creation of more efficient rehabilitation program by using Motor Assessment Scale (MAS). Twenty-four stroke patients (14 men and 10 women) were the subjects in this study. Their average age was 59.5 and they received average of 17.88 month of therapy. Collected data analysis was completed by using Statistic Analysis System (SAS). The results were as follows: 1) There was no difference in capacity of activity between right hemiplegia and left hemiplegia. 2) There was no difference in capacity of activity compared therapeutic period and age. 3) In comparing the relationship of the each subtest with the overall capacity of activity, upper arm function showed the highest relation (pearson's r = 0.914), and balance sitting (pearson's r= 0.812) and supine to sitting overside of bed (pearson'sr = 0.746) also showed large relationship. 4) Hand movement (pearson's r = -0.45) and advanced hand activity (pearson's r = -0.401) revealed relationship of general tonus with each subtest. 5) Supine to sitting over side of bed (pearson's r = 0.74), balanced sitting(pearson's r = 0.523), and sitting to standing (pearson's r = 0.723) showed large relationship with walking.

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Screening and Identification of the Fibrinolytic Bacterial Strain from Jeot-Gal, Salt-fermented Fish (젓갈류로부터 혈전용해 균주의 분리 및 동정)

  • Jang, Young-Ryeol;Kim, Won-Keuk;Kwon, Ik-Boo;Lee, Hyun-Yong
    • Korean Journal of Food Science and Technology
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    • v.30 no.3
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    • pp.655-659
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    • 1998
  • Bacterial strain showing the strong fibrinolytic activity (2.04 plasmin unit) was screened from Jeot-Gal, Korean salt-fermented fish collected from various region. For the identification, when the strain was characterized morphologically, culturally, and biochemically, it was identified to Bacillus pumilus. And, when the fatty acids composition of the strain was analyzed, it was identified to Bacillus atropheus. Finally, the 16S rRNA partial sequence (V3 region) showed that the fibrinolytic stain screened from Jeot-Gal was identified as Bacillus subtilis. So, we named it Bacillus subtilis KJ-48.

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Measurement of Saw-Teeth Wear by TALYSURF (TALYSURF에 의한 톱니의 마모량측정)

  • Hyun, Jung-Ihn;Klamecki, Barney E.
    • Journal of the Korean Wood Science and Technology
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    • v.8 no.1
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    • pp.22-27
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    • 1980
  • Quantitative assessment of edge blunting of saw-teeth was carried out by TALYSURF. 1. Using the following equation, the real shape of a saw-tooth can be traced on the graph of TALYSURF. ${\frac{{\Delta}h}{h}}={\frac{V{\Delta}_x}{V_x}}$ {${\Delta}h$: vertical distance of stylus h: vertical distance in chart $V{\Delta}_x$: Velocity of stylus $V_x$: velocity of chart} 2. As shown on Fig 2, the error from stylus itself can be calculated by following equation. i) 13.8${\mu}{\leqq}$x<20.4${\mu}$ y=-0.2246x+4.59${\mu}$ ii) 0${\leqq}$x<13.8${\mu}$ y=${\sqrt{(-18{\mu})^2-x^2}}-1.42x+32.7{\mu}}$ 3. The relationship between profile of saw-tooth and error from stylus itself can be calculated by following equation. $E(%)=\frac{f(r){\times}{\frac{4}{18{\mu}}}}{f(R){\times}{\frac{R}{18.5{\mu}}}-f(r){\times}{\frac{r}{18{\mu}}}}{\times}100$ {E(%)${\frac{error\;of\;stylus}{dullness\;of\;saw\;tooth}}{\times}100$ r: radius of stylus tip R: radius of tip which is drawn in graph of talysurf f(r) : error of stylus f(R) : dullness of tip which is drawn in graph of talysurf} 4. The graph of maximum error and profile of saw-tooth was parabola.

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