Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Screening and Identification of the Fibrinolytic Bacterial Strain from Jeot-Gal, Salt-fermented Fish

젓갈류로부터 혈전용해 균주의 분리 및 동정

  • Jang, Young-Ryeol (Department of Biotechnology, Lotte Group Institute of R&D) ;
  • Kim, Won-Keuk (Department of Biotechnology, Lotte Group Institute of R&D) ;
  • Kwon, Ik-Boo (Department of Biotechnology, Lotte Group Institute of R&D) ;
  • Lee, Hyun-Yong (Division of Food Science & Biotechnology, Kangwon National University)
  • Published : 1998.06.01
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Abstract

Bacterial strain showing the strong fibrinolytic activity (2.04 plasmin unit) was screened from Jeot-Gal, Korean salt-fermented fish collected from various region. For the identification, when the strain was characterized morphologically, culturally, and biochemically, it was identified to Bacillus pumilus. And, when the fatty acids composition of the strain was analyzed, it was identified to Bacillus atropheus. Finally, the 16S rRNA partial sequence (V3 region) showed that the fibrinolytic stain screened from Jeot-Gal was identified as Bacillus subtilis. So, we named it Bacillus subtilis KJ-48.

우리나라 각지에서 젓갈류를 수집하여 혈전용해능을 나타내는 균주들을 분리하였다. 분리된 균주들의 활성을 측정한 결과 2.04 plasmin unit의 가장 우수한 혈전용해활성을 나타내는 균주를 선별하여 동정하였다. 균주의 형태학적, 배양학적 및 생화학적 성질을 조사한 결과 Bacillus pumilus로 나타났으나, 균체의 지방산조성을 분석하여 동정한 결과 Bacillus atropheus로 나타났다. 최종적 결론을 얻기 위해, 16S rRNA partial sequence(V3 region)를 결정하여 분석한 결과 Bacillus subtilis의 16S rRNA partial sequence와 동일한 것으로 나타나, Bacillus subtilis KJ-48로 명명하였다.

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