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Karyotype Analysis and Physical Mapping of rDNAs in Bupleurum longeradiatum  

Koo, Dal-Hoe (Department of Biology, Chungnam Natl. Univ.)
Seong, Nak-Sul (National Crop Experimental Station, RDA)
Seong, Jong-Suk (National Crop Experimental Station, RDA)
Bang, Kyong-Hwan (National Crop Experimental Station, RDA)
Bang, Jae-Wook (Department of Biology, Chungnam Natl. Univ.)
Publication Information
Korean Journal of Medicinal Crop Science / v.11, no.5, 2003 , pp. 402-407 More about this Journal
Abstract
Karyotype analysis and chromosomal localization of 5S and 45S rDNAs using multi-color fluorescence in situ hybridization (McFISH) technique were carried out in Bupleurum longeradiatum. Somatic metaphase chromosome number was 2n=12. Karyotype was composed of three pairs of metacentrics (No.3, 4 and 6) and three pairs of submetacentrics (No. 1, 2 and 5). The length of somatic prometaphase chromosomes ranges from 2.55 to $5.05{\mu}m$ with total length of $18.15\;{\mu}m$. In FISH experiment, one pair of 5S rDNA signals was detected on the pericentromeric region of chromosome 4 and one pair of 45S rDNA signals was detected on the telomeric region of chromosome 2.
Keywords
Bulpleurum longeradiatum; karyotype; multi-color fluorescence in situ hybridization; rDNA;
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