• Title/Summary/Keyword: S&T community

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T-RFLP Analysis of Microbial Community Structure in Leachate from Landfill Sites (폐기물매립장 침출수내 미생물군집 구조 해석을 위한 T-RFLP의 활용)

  • Yu, Jae-Cheul;Ishigaki, Tomonori;Kamagata, Yoichi;Lee, Tae-Ho
    • Journal of Korean Society of Environmental Engineers
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    • v.32 no.4
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    • pp.369-378
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    • 2010
  • Microorganisms are key-role player for stabilization of landfill sites. In order to evaluate the availability of T-RFLP(Terminal Restriction Fragment Length Polymorphism) for monitoring microbial community variations during stabilization of landfill sites, the phylogenic diversity of microbial community in the leachate from 4 different full-scale landfills was characterized by T-RFLP based on bacterial 16S rDNA. Main population of microbial community analyzed by T-RFLP was significantly similar with that of microbial community analyzed by clone library analysis. The results of T-RFLP analysis for main population of microbial community in the leachate from landfills with different landfill structures, waste types and landfill ages showed apparently different microbial diversity and structures. Therefore, long-term monitoring of microbial community in leachate from landfill sites by using T-RFLP is expected to be available for evaluation of landfill stability.

Statistical Hierarchical Analysis of Children Emotional Intelligence's Effects on Mural Preference, Emotion Cultivation, and Community Connection

  • Lee, Kang Il;Ko, Young Chun
    • Journal of Integrative Natural Science
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    • v.7 no.1
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    • pp.50-56
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    • 2014
  • To explore effects on each the emotional awareness, emotional expression, emotional empathy, and emotional regulation, of the sub-factors of the child's emotional intelligence, to mural preference, emotion cultivation, and community connection, the hierarchical multiple regression analyses are performed(as in Table 1, 2, and 3). As the results, we found the following facts. Children's mural preference, emotion cultivation, and community connection were expressed by the following equations in order, respectively. Mural Preference = $.170{\times}$[Emotional Awareness](t=2.118, $p=.036^*$) - $.025{\times}$[Emotional Expression](t=-.275, p=.783) + $.088{\times}$[Emotional Empathy](t=.938, p=.350) + $.139{\times}$[Emotional Regulation] (t=1.529, p=.128). Mural Emotion Cultivation = $-.021{\times}$[Emotional Awareness](t=-.294, p=.769) - $.205{\times}$[Emotional Expression](t=-2.573, $p=.011^*$) + $.265{\times}$[Emotional Empathy](t=3.156, $p=.002^*$) + $.192{\times}$[Emotional Regulation](t=2.361, $p=.019^*$). Mural Community Connection = $-.001{\times}$[Emotional Awareness](t=-.007, p=.995) - $.132{\times}$[Emotional Expression](t=-1.478, p=.141) + $.172{\times}$[Emotional Empathy](t=1.732, $p=.027^*$) + $.098{\times}$[Emotional Regulation](t=1.072, p=.285).

Comparison of Terminal-restriction Fragment Length Polymorphism (T-RFLP) Analysis and Sequencing of 16S rDNA Clones in marine sediments

  • Lee Jung-Hyun
    • Proceedings of the Microbiological Society of Korea Conference
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    • 2002.10a
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    • pp.15-21
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    • 2002
  • Terminal-restriction fragment length polymorphism (T-RFLP) analysis has been optimized by using in vitro model community composed of genomic DNAs of known bacterial strains and has been applied to assess the bacterial community structure in marine sediments. The specific fluorescence-labeled terminal restriction fragments (T-RFs) between 39 and 839 base long specifying each strain were precisely measured for known bacterial strains. The addition of a co-solvent (dimethylsulfoxide or glycerol) into PCR reactions has reduced differential PCR amplification. Comparative bacterial community structure was investigated for pristine and polluted sediments. A complex T-RFLP pattern showing complex bacterial community structure was obtained in the pristine sediment, whereas simple T-RFLP pattern (low bacterial diversity) was shown in polluted sediments where caged aquaculture has been conducted for several years. The results of T-RFLP analysis were compared with that of cloning and sequencing 16S rDNA clones from the same sediments. Sequence analysis of 16S rDNA clones (72) of the pristine sediment revealed a diverse collection of lineages, largely of the class Proteobacteria ($6\%$ alpha subdivision, $46\%$ gamma subdivision, $13\%$ delta subdivision, and $3\%$ epsilon subdivision), Nitrospina $(8\%)$, high G+C gram positive $(8\%)$, Verrucomicrobia $(7\%)$, and Planctomycetes $(6\%)$. In the contaminated sediments, 17 $(59\%)$ of the 16S rDNA clones (29) were related to Campylobacter and symbiont of Rimicaris exoculata belonging to epsilon subdivision of Proteobacteria. The results obtained indicated that T-RFLP analysis is a rapid and precise technique for comparative bacterial community analysis.

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Use of Terminal Restriction Length Polymorphism (T-RFLP) Analysis to Evaluate Uncultivable Microbial Community Structure of Soil

  • Chauhan, Puneet Singh;Shagol, Charlotte C.;Yim, Woo-Jong;Tipayno, Sherlyn C.;Kim, Chang-Gi;Sa, Tong-Min
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.1
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    • pp.127-145
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    • 2011
  • Various environmental ecosystems are valuable sources for microbial ecology studies, and their analyses using recently developed molecular ecological approaches have drawn significant attention within the scientific community. Changes in the microbial community structures due to various anthropogenic activities can be evaluated by various culture-independent methods e.g. ARISA, DGGE, SSCP, T-RFLP, clone library, pyrosequencing, etc. Direct amplification of total community DNA and amplification of most conserved region (16S rRNA) are common initial steps, followed by either fingerprinting or sequencing analysis. Fingerprinting methods are relatively quicker than sequencing analysis in evaluating the changes in the microbial community. Being an efficient, sensitive and time- and cost effective method, T-RFLP is regularly used by many researchers to access the microbial diversity. Among various fingerprinting methods T-RFLP became an important tool in studying the microbial community structure because of its sensitivity and reproducibility. In this present review, we will discuss the important developments in T-RFLP methodology to distinguish the total microbial diversity and community composition in the various ecosystems.

Social network analysis of keyword community network in IoT patent data (키워드 커뮤니티 네트워크의 소셜 네트워크 분석을 이용한 사물 인터넷 특허 분석)

  • Kim, Do Hyun;Kim, Hyon Hee;Kim, Donggeon;Jo, Jinnam
    • The Korean Journal of Applied Statistics
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    • v.29 no.4
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    • pp.719-728
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    • 2016
  • In this paper, we analyzed IoT patent data using the social network analysis of keyword community network in patents related to Internet of Things technology. To identify the difference of IoT patent trends between Korea and USA, 100 Korea patents and 100 USA patents were collected, respectively. First, we first extracted important keywords from IoT patent abstracts using the TF-IDF weight and their correlation and then constructed the keyword network based on the selected keywords. Second, we constructed a keyword community network based on the keyword community and performed social network analysis. Our experimental results showed while Korea patents focus on the core technologies of IoT (such as security, semiconductors and image process areas), USA patents focus on the applications of IoT (such as the smart home, interactive media and telecommunications).

Phytosociological Studies on the Beech(Fagus multinervis Nakai) Forest and the Pine (Pinus parviflora S. et Z.) Forest of Ulreung Island, Korea (한국 울릉도의 너도밤나무(Fagus multinervis Nakai)림 및 섬잣나무(Pinus parviflora S. et Z.)림의 식물사회학적 연구)

  • 김성덕
    • Journal of Plant Biology
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    • v.29 no.1
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    • pp.53-65
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    • 1986
  • The montane forests of Ulreung Island, Korea, were investigated by the ZM school method. By comparing the montane forests of this island with those of Korean Peninsula and of Japan, a new order, F a g e t a l i a m u l t i n e r v i s, a new alliance, F a l g i o n m u l t i n e r v i s, a new association, H e p a t i c o-F a g e t u m m u l t i n e r v i s and Rhododendron brachycarpum-Pinus parviflora community were recognized. The H e p a t i c o - F a g e t u m m u l t i n e r v i s was further subdivided into four subassociations; Subass. of Sasa kurilensis, Subass. of Rumohra standishii, Subass. of Rhododendron brachycarpum and Subass. of typicum. Each community was described in terms of floristic, structural and environmental features.

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The Effect of Public Health Center-Based Hypertension School on Hypertension-related Knowledge, Self-efficacy, Anthropometric Value and Blood Pressure

  • Chang, Koungoh;Kim, Sohee;Lee, Naeyoung
    • International journal of advanced smart convergence
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    • v.7 no.3
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    • pp.44-60
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    • 2018
  • This study is to identify the effects of hypertension management program at a community health center on the disease-related knowledge, self-efficacy, anthropometric measurements and blood pressure of a hypertensive patient in local community. This study is a quasi-experimental study using nonequivalence control group no-synchronized design in order to verify the effects of the hypertension management program at a community health center on the hypertension-related knowledge, self-efficacy, anthropometric measurements and blood pressure of a hypertensive patient in local community. The result indicated a significant difference between the experimental group and control group in the scores of hypertension-related knowledge (t=-4.25, p<.001), self-efficacy (t=-4.20, p<.001), systolic blood pressure (t=7.70, p<.001) and diastolic blood(t=5.91, p<.001), body weight(t=2.32, p=.026) and abdominal circumference(t=2.17, p=.036). The hypertensive patients' knowledge and self-efficacy were improved, and their weight and abdominal circumference as well as systolic blood pressure and diastolic blood pressure were reduced. Therefore, it was confirmed that multilateral approaches in terms of physical and psychosocial aspects only targeting hypertensive patients were required for managing hypertensive patients in local community.

Plant Community Structure from the Jilmoi Wetlands to the Donghae Observatory, Baekdudaegan Mountains

  • Choi, Jin-Woo;Kim, Kyung-Won;Yeum, Jung-Hun;Hwang, Won-Seok
    • Korean Journal of Environment and Ecology
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    • v.29 no.2
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    • pp.250-262
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    • 2015
  • This study aims to investigate the characteristics of the vegetation structure in the sectin stretching between the Jilmoi wetlands and the Donghae Observatory and to set the criteria for the basic data for a management plan including restoration afterwards. 12 plots($10m{\times}40m$, $20m{\times}20m$) were set up to analyse the vegetation structure. The analysis of the classification by TWINSPAN and ordination by DCA, importance percentage and property, distribution of diameter of breast height, growth increments of major woody species, species diversity and the physicochemical properties of soil were all analyzed. Vegetation classes were divided into 3 communities, which are community I (Pinus densiflora community), community II (Quercus mongolica community) and community III (Quercus mongolica-Tilia amurensis community). The P. densiflora community declined when competing with Q. mongolica and Fraxinus rhynchophylla and Q. mongolica competed with T. amurensis on an understory layer in Q. mongolica community. Q. mongolica competed with T. amurensis on both canopy and understory layers in Q. mongolica-T. amurensis community. P. densiflora declined and it was assumed to succeed to F. rhynchophylla or T. amurensis through Q. mongolica based on the importance percentage and distribution of the diameter of the breast height of small and middle sized trees. The age of P. densiflora was between 47 to 51 years old and Q. mongolica was 61years old. T. amurensis was 61 years old and the growth of Q. mongolica slowed a little. As the result of Shannon's index of species diversity, community I ranged from 0.9578 to 1.1862, community II ranged from 0.7904 to 1.2286 and community III ranged from 0.8701 to 1.0323. The contents of organic matter and cation were low compared to uncultivated mountain soil and it were analysed to be inappropriate for tree growth.

Differentiated Community of Scientists and Engineers in Korea (이공계 기피 논의를 통해 본 한국 과학기술자 사회의 특성)

  • Lee, Eun-Kyoung
    • Journal of Science and Technology Studies
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    • v.6 no.2 s.12
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    • pp.77-102
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    • 2006
  • This paper analyzes shaping of the discourse of "avoidance of S&T" throughout 2002 in Korea and main ideas on it and argues that the Korean S&T community is not homogeneous. The Korean S&T community consists of many subgroups with different interest mainly according to the institutions where they work as well as their specialties, qualifications, and tasks. The discourse of avoidance of S&T" was begun with the first notice of the rapid decrease in applications of Korean SAT for S&T majors in the middle of 2001. The decrease was interpreted as a warning sign of the avoidance of S&T among teenagers and developed into the avalanche of discussions on the causes of such rapid decrease around the end of the year. However, through 2002, the "avoidance of S&T' was developed into a general policy agenda covering most of problems in S&T, not just a teenagers' issue any more. Many scientists and engineers claimed that their own difficulties and problems be the main causes of the "avoidance of S&T". In the name of measures to overcome the avoidance, they requested the government to solve their problems and to accept their demands. Those claims varied from group to group. Some of them were shared among all scientists and engineers; some of them were criticized by other groups with different interest in it; some of them did not get any attention of other groups. Scientists and engineers seemed to have no idea on cooperative strategy to draw positive response to their requests from the government.

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A Study on Direction for Rural Community Building through the Case of Gurye-County (구례군의 사례를 통하여 본 농촌마을만들기의 방향)

  • Seo, Chung Ho
    • Journal of Korean Society of Rural Planning
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    • v.19 no.1
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    • pp.33-41
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    • 2013
  • Community building has been carried out from around the year 2000 by various statutes and government policies in Korea. This study suggested direction of the future for rural community building as surveyed cases of completed four villages in Gurye-county. In order to achieve this study's objectives, investigated previous studies and interviewed 75 person's residents in cases villagers. The target villages are Sangsa, Omi, Chamsaimigol and Artists village. As survey results, four villages utilized local resources, most of villages were focused perform hardware. On the other hand, Chamsaimigol was parallel software. In addition, communities didn't form and not agree with concept of community building as culture, education, welfare, etc., also didn't creation the case of village income. but active village as base development. Therefor, proposed the point to education, culture and welfare, base development and generating of income as directions for future rural community building. These directions are consistent with the concept of community building and the goal that improve the quality of life of residents ultimately through community building.