• Mycobiology
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• v.49 no.6
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• pp.599-603
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• 2021

CRISPR/Cas9 genome editing systems have been established in a broad range of eukaryotic species. Herein, we report the first method for genetic engineering in pyogo (shiitake) mushrooms (Lentinula edodes) using CRISPR/Cas9. For in vivo expression of guide RNAs (gRNAs) targeting the mating-type gene HD1 (LeA1), we identified an endogenous LeU6 promoter in the L. edodes genome. We constructed a plasmid containing the LeU6 and glyceraldehyde-3-phosphate dehydrogenase (LeGPD) promoters to express the Cas9 protein. Among the eight gRNAs we tested, three successfully disrupted the LeA1 locus. Although the CRISPR-Cas9-induced alleles did not affect mating with compatible monokaryotic strains, disruption of the transcription levels of the downstream genes of LeHD1 and LeHD2 was detected. Based on this result, we present the first report of a simple and powerful genetic manipulation tool using the CRISPR/Cas9 toolbox for the scientifically and industrially important edible mushroom, L. edodes.

• Korean Journal of Agricultural Science
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• v.49 no.2
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• pp.285-296
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• 2022

Food consumption trends in Korea are becoming more health-oriented, and consumers have become interested in high-quality foods that are also beneficial to health. The goals of this study are to examine consumers' impressions of black garlic and to investigate the preferred optimal attribute combinations related to black garlic. To do this, a survey of 352 consumers was used to analyze the current status of consumer purchases of black garlic. A conjoint analysis was conducted to analyze certain aspects of consumer preferences, such as price, health functional food certification status, processing type, and taste. Consumers' purchase experiences with black garlic were relatively few, but those with purchase experience reported that they consumed it mainly for their health. The results show that consumers are not satisfied with the taste and flavor of black garlic despite their perceiving it as a beneficial food for health. Despite the fact that consumers' purchase intentions were found to be low, it can greatly increase if improvements in price, flavor, and taste can be realized and if offering substantial benefits. The results of the conjoint analysis of the preference attributes of black garlic products are as follows. Consumers considered price among the four attributes as most important. The taste of black garlic and the processing style were similar in terms of importance, and health functional food certification was less important. Finally, the results suggest that continuous improvements in price and flavor are needed to spread the consumption of black garlic.

• Natural Product Sciences
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• v.28 no.4
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• pp.181-186
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• 2022

A germacrane-type sesquiterpenoid was isolated and purified from a methanol extract of the roots of Valeriana fauriei (RVF) through open column chromatography using silica gel. This compound was verified to be heishuixiecaoline A by spectroscopic analysis. This compound was isolated for the first time from RVF. Quantitative analysis of heishuixiecaoline A from RVF cultivated from three different regions (Eumseong, Jinbu, and Jinan regions) was performed by combining high-performance liquid chromatography with a photodiode array detector. The extract of RVF cultivated in the Jinbu region showed the highest content (9.23 mg/g). In addition, a significant amount of the compound was detected in all RVF samples, which could be expected since it is a characteristic compound of RVF. The sesquiterpenoid group heishuixiecaoline A was isolated from RVF, a resource for various pharmacological substances, and quantitative analysis of RVF cultivated from three different regions was performed. As a result of these experiments, basic data on RVF that can be used in the development and application of pharmaceuticals and health functional foods in the future were obtained.

• The Korean Journal of Mycology
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• v.50 no.3
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• pp.235-241
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• 2022

Anthracnose crown rot (ACR) has been observed in greenhouses during the nursery and harvest seasons in Gangwon Province, Korea. Infected plants showed black leaf spot, dark sunken pink conidial masses on petioles, wilting, and eventually death. Five isolates were obtained from the lesions of strawberry plants and were identified as a Colletotrichum gloeosporioides species complex based on their cultural and morphological characteristics. Multilocus sequence analysis of actin, calmodulin, chitin synthase, glyceraldehyde-3-phophate dehydrogenase genes, and internal transcribed spacer rDNA regions showed that the isolates formed a monophyletic group with the type strain of C. siamense. Pathogenicity tests were performed on the isolate, and Koch's postulates were performed to verify the relationship between Colletotrichum sp. and the strawberry plant variety Seolhyang. The isolate was pathogenic to strawberry plants, which exhibited typical ACR symptoms. Based on morphological characteristics, pathogenicity, and DNA sequence analyses, the fungus isolated in Korea was identified as C. siamense. This is first time C. siamense has been confirmed in ever-bearing strawberry varieties in Korea.

• IMMUNE NETWORK
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• v.20 no.4
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• pp.35.1-35.13
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• 2020

Antigen delivery systems play critical roles in determining the quality and quantity of Ab responses in vivo. Induction of protective antibodies by B cells is essential in the development of vaccines against infectious pathogens, whereas production of IgE antibodies is prerequisite for investigation of allergic responses, or type 1 hypersensitivity reactions. Virus-like particles (VLPs) are efficient platforms for expression of proteins of interest in highly repetitive manners, which grants strong Ab responses to target antigens. Here, we report that delivery of hen egg lysozyme (HEL), a model allergen, through VLP could provoke strong HEL specific IgE Ab responses in mice. Moreover, acute allergic responses were robustly induced in the mice sensitized with VLPs that express HEL, when challenged with recombinant HEL protein. Our data show that antigen delivery in the context of VLPs could function as a platform for sensitization of mice and for subsequent examination of allergic reactions to molecules of interest.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.2
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• pp.266-271
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• 2012

This study was carried out to evaluate the land suitability for southern-type garlic cultivation associated with both temperature and soil constraints. The suitability analysis was conducted with hourly temperature data from 2001 to 2010 at all fifty seven meteorological stations and the soil-based suitability map of garlic provided by Rural Development Administration. Firstly the temperature data were processed by the growth stages (germinating, bulbing, and winter vegetation season), and then were adopted to limit the irrelevant lands. Next, as a result of overlaying each soil and temperature suitability map, the total 274,339 ha of area was mapped as highly suitable or suitable for southern-type garlic cultivation and the top four of the largest classified si-guns were identified as Naju, Jeongeup, Gochang, and Jinju. On the other hand, the statistical records of KOSIS (KOrea Statistical Information Service) showed lower amount of cultivation area than the analyzed results in the major production sites, Goheung, Sinan, Haenam, and Muan. However, it should not be regarded as exceptional because farmer's preference might not correspond to potential land usability.

• Korean Journal of Community Nutrition
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• v.12 no.1
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• pp.90-105
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• 2007

This study was conducted to assess macro- and micronutrient compositions in school lunch menus based on the 'Dietary Guideline' for School Lunches. Ninety-five dieticians in elementary school in Chungbuk were asked to complete a questionnaire on characteristics of the school lunch program(such as type of foodservice, food production system), the information about dietitians(such as age, education, and job history), and the extent of the use of processed foods and frozen foods. Dey were asked to return the questionnaire with the menus including the name and the quantity of every food ingredient offered during a week. A total of 554 lunch menus provided for a week of June 2004 were analyzed. Average nutrient content per meal was as follows; 660kcal, energy; 92.9g, carbohydrate; 26.7g, protein; 21.1g, fat; 287 ${\mu}gRE$, vitamin A: 0.5mg, thiamin; 0.5mg, riboflavin; 29.3mg, vitamin C: 338.2mg, calcium; 3.9mg, iron; and 97mg, cholesterol. Average percentages of energy from carbohydrate, protein and fat was 56.2%, 16.2%, and 29.0%, respectively. The mean nutrient content per meal was higher in rural-type than in urban-type schools. The weekly menu of 40% of the schools provided <55% of energy from carbohydrate, and 39% of the schools offered lunch that provided ${\geq}30%$ of energy from fat. The micronutrient content was generally high when the percent energy from fat was less than 25%. Our results showed that only 52.6% of the schools provided lunches with the energy composition as in the 'Dietary Guideline' of School Lunches. Whole Milk was the major contributor to fat, saturated fatty acid and cholesterol. We suggest that school foodservices start to provide low fat milk instead of whole milk to reduce fat, saturated fatty acid and cholesterol. If low fat milk is served instead of whole milk, percentage of energy from fat and saturated fat can be reduced from 29% to 25%, and ken 10.2% to 9.1%, respectively, and cholesterol could be reduced from 97mg to 79mg. Efforts to meet 'Dietary Guideline' for School Lunches should be made, especially to reduce fat intake, while maintaining essential nutrient intake at sufficient levels for childen.

• Horticultural Science & Technology
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• v.31 no.3
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• pp.388-392
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• 2013

A spray chrysanthemum cultivar 'Green Candy' was released by National Institute of Horticultural & Herbal Science, Rural Development Administration, in 2009. The cross between 'Yoko Ono', a spray chrysanthemum cultivar with light yellowish-green double type, and 'Green Bird', a spray chrysanthemum cultivar with vigorous and green double type, was made in Suwon in 2006. After the evaluation of the characteristics under shade culture in summer and retarding culture in spring and consecutive selection from 2007 to 2009, 'Green Candy' was selected finality. The natural flowering time of 'Green Candy' is October 28th, and it is possible to flower all year-round by shade and light culture. It has double flower type with green petals. The growth of plant is very vigorous and the color of the petals is stable when the variety is cultivated under high temperature conditions in summer season. The diameter of flower is 4.1 cm. The numbers of flower per stem and petals per flower are 12 and 217, respectively. The days for flowering under the short day treatment are about 58 and its vase life is 18.1 days in autumn season. 'Green Candy' was applied as No. 2010-212 on March 5, 2010 for variety protection and the plant variety protection rights have been registered as No. 3633 on August 2, 2011 at the Korea Seed and Variety Service.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.2
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• pp.511-520
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• 2020

Owing to global warming, heat waves have become stronger in the summer, and research on improving the thermal environment of green spaces, such as urban parks, is being conducted. On the other hand, studies on improving the urban thermal environment, which is changing due to the greening pattern and the intensity of the wind, are still insufficient. This study analyzed the temperature of the green spaces on campus to understand the factors affecting the temperature changes. After investigating the covering condition and planting form of the site, factors, such as temperature, humidity, wind direction, wind speed, and illuminance, were measured. The most influential factors on the temperature distribution are evapotranspiration and wind - induced heat transfer. The other major factors affecting the temperature change were the type of cover, wind velocity/wind direction, type of planting, shade / solar irradiance. In the type of cover, the plant was classified as low temperature, and the asphalt pavement was classified as high temperature. In wind speed, instantaneous temperature was reduced by 1.2 ℃ in southern wind, 0.7 ℃ in the westerly wind, 0.4 ℃ in the north wind and 0.5 ℃ in the east wind when a wind of 3.5m/s or more was blown.

• Journal of Sericultural and Entomological Science
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• v.47 no.2
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• pp.93-98
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• 2005

The antioxidant capacity of yield-type mulberry leaves and fruits (Morus alba L., M. bombycis Koidz, and M. Lhou (Ser). Koidz) collected from In-je, Won-ju and Yang-yang regions, Kang-won province, Korea, was investigated with minium L-100 device and ARAW-KIT (anti-radical ability of water-soluble substance), in comparison to the ascorbic acid. The results indicated that total antioxidant capacity of yield-type mulberry leaves was 2711.2 nmol (ascorbic acid equivalents). The highest stage of antioxidant capacity (3587.6 nmol) was opening stage of five leaves in spring. Especially, antioxidant capacity of mulberry leave collected from In-je region was high than that of other regions. No. 7 mulberry leave showed the strongest antioxidant capacity (6184.9 nmol) at opening stage of five leaves in spring. Also, fluit growing condition including fruit size and yield was good, too. Therefore, No. 7 mulberry three from In-je region, possessed not only high antioxidant capacity but also considerable fruit quality, was selected as potent resource for mulberry breeding and functional material development.