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Virus-like Particle (VLP) Mediated Antigen Delivery as a Sensitization Tool of Experimental Allergy Mouse Models

  • Juhyung Kim (Department of Biomedical Sciences, Seoul National University College of Medicine) ;
  • Jeein Oh (Department of Biomedical Sciences, Seoul National University College of Medicine) ;
  • Chon-Sik Kang (National Institute of Crop Science, Rural Development Administration (RDA)) ;
  • Youn Soo Choi (Department of Biomedical Sciences, Seoul National University College of Medicine)
  • Received : 2020.03.10
  • Accepted : 2020.07.08
  • Published : 2020.08.31
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Abstract

Antigen delivery systems play critical roles in determining the quality and quantity of Ab responses in vivo. Induction of protective antibodies by B cells is essential in the development of vaccines against infectious pathogens, whereas production of IgE antibodies is prerequisite for investigation of allergic responses, or type 1 hypersensitivity reactions. Virus-like particles (VLPs) are efficient platforms for expression of proteins of interest in highly repetitive manners, which grants strong Ab responses to target antigens. Here, we report that delivery of hen egg lysozyme (HEL), a model allergen, through VLP could provoke strong HEL specific IgE Ab responses in mice. Moreover, acute allergic responses were robustly induced in the mice sensitized with VLPs that express HEL, when challenged with recombinant HEL protein. Our data show that antigen delivery in the context of VLPs could function as a platform for sensitization of mice and for subsequent examination of allergic reactions to molecules of interest.

Keywords

Acknowledgement

This work was supported by the Cooperative Research Program for Agriculture Science and Technology Development funded to Choi YS (PJ012527022018) by Rural Development Administration.

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